Capybara microvirus Cap3_SP_379
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W8G3|A0A4P8W8G3_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_379 OX=2585438 PE=4 SV=1
MM1 pKa = 7.71 KK2 pKa = 10.63 YY3 pKa = 10.17 LVTSATIAHH12 pKa = 6.7 YY13 pKa = 10.22 DD14 pKa = 3.44 SNMFEE19 pKa = 4.79 SDD21 pKa = 3.11 NEE23 pKa = 4.24 EE24 pKa = 3.95 NFKK27 pKa = 11.39 AEE29 pKa = 3.79 TDD31 pKa = 4.25 DD32 pKa = 3.76 IVTARR37 pKa = 11.84 IIAHH41 pKa = 6.1 TFAKK45 pKa = 10.15 ILCDD49 pKa = 3.69 YY50 pKa = 9.36 EE51 pKa = 5.09 NNIAGYY57 pKa = 10.06 GEE59 pKa = 3.48 IWEE62 pKa = 4.3 EE63 pKa = 4.0 NKK65 pKa = 10.49 NKK67 pKa = 9.62 QLEE70 pKa = 4.44 SIEE73 pKa = 3.88 EE74 pKa = 4.12 KK75 pKa = 10.46 IYY77 pKa = 10.32 FKK79 pKa = 11.06 PNN81 pKa = 2.84
Molecular weight: 9.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.532
IPC2_protein 4.622
IPC_protein 4.469
Toseland 4.342
ProMoST 4.571
Dawson 4.406
Bjellqvist 4.558
Wikipedia 4.266
Rodwell 4.317
Grimsley 4.253
Solomon 4.406
Lehninger 4.355
Nozaki 4.52
DTASelect 4.622
Thurlkill 4.342
EMBOSS 4.279
Sillero 4.584
Patrickios 3.528
IPC_peptide 4.418
IPC2_peptide 4.584
IPC2.peptide.svr19 4.512
Protein with the highest isoelectric point:
>tr|A0A4P8W4V1|A0A4P8W4V1_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_379 OX=2585438 PE=4 SV=1
MM1 pKa = 7.81 NYY3 pKa = 8.78 MCLYY7 pKa = 8.77 PRR9 pKa = 11.84 KK10 pKa = 9.49 IRR12 pKa = 11.84 NPRR15 pKa = 11.84 YY16 pKa = 9.51 AAGNKK21 pKa = 9.32 NGYY24 pKa = 7.52 SQEE27 pKa = 3.98 DD28 pKa = 3.9 CKK30 pKa = 11.18 DD31 pKa = 3.07 KK32 pKa = 11.02 RR33 pKa = 11.84 KK34 pKa = 10.37 LYY36 pKa = 10.21 IQIDD40 pKa = 3.72 CGNCEE45 pKa = 3.55 EE46 pKa = 4.68 CKK48 pKa = 9.97 RR49 pKa = 11.84 KK50 pKa = 9.64 KK51 pKa = 10.36 GKK53 pKa = 8.24 WWTIRR58 pKa = 11.84 IMEE61 pKa = 4.35 EE62 pKa = 3.57 LKK64 pKa = 10.51 INKK67 pKa = 7.93 GTFVTLTFSANSLIEE82 pKa = 3.87 LTKK85 pKa = 10.68 GEE87 pKa = 4.31 KK88 pKa = 10.15 VKK90 pKa = 10.64 LANEE94 pKa = 4.23 TATKK98 pKa = 9.97 AVRR101 pKa = 11.84 RR102 pKa = 11.84 FLEE105 pKa = 4.37 RR106 pKa = 11.84 YY107 pKa = 8.17 RR108 pKa = 11.84 KK109 pKa = 9.55 KK110 pKa = 9.76 HH111 pKa = 4.03 GHH113 pKa = 4.76 SVKK116 pKa = 10.5 HH117 pKa = 5.81 ILITEE122 pKa = 4.66 LGDD125 pKa = 3.49 EE126 pKa = 4.6 EE127 pKa = 5.06 NEE129 pKa = 4.18 HH130 pKa = 6.23 TGRR133 pKa = 11.84 LHH135 pKa = 6.07 LHH137 pKa = 6.97 GILFDD142 pKa = 4.49 TITKK146 pKa = 10.57 EE147 pKa = 4.17 EE148 pKa = 4.36 LNEE151 pKa = 3.57 LWKK154 pKa = 11.19 YY155 pKa = 11.04 GFVDD159 pKa = 3.43 TGNYY163 pKa = 9.34 CNEE166 pKa = 3.61 RR167 pKa = 11.84 TANYY171 pKa = 5.56 ITKK174 pKa = 10.58 YY175 pKa = 9.97 ILKK178 pKa = 9.92 LDD180 pKa = 4.1 KK181 pKa = 10.94 NHH183 pKa = 7.12 PNYY186 pKa = 9.15 KK187 pKa = 9.98 QKK189 pKa = 10.64 IFVSAGLGKK198 pKa = 10.32 NYY200 pKa = 9.82 TEE202 pKa = 3.83 QAQRR206 pKa = 11.84 QKK208 pKa = 10.79 NKK210 pKa = 10.27 SYY212 pKa = 11.01 RR213 pKa = 11.84 FTNGCQFPLPTYY225 pKa = 9.62 YY226 pKa = 10.48 KK227 pKa = 9.83 NKK229 pKa = 9.42 VFSEE233 pKa = 4.37 EE234 pKa = 3.83 EE235 pKa = 4.09 RR236 pKa = 11.84 EE237 pKa = 4.32 QMWTEE242 pKa = 3.66 QLDD245 pKa = 4.0 KK246 pKa = 11.38 NEE248 pKa = 4.75 HH249 pKa = 5.82 YY250 pKa = 10.95 VSGMTFRR257 pKa = 11.84 FNNTKK262 pKa = 10.59 RR263 pKa = 11.84 NLKK266 pKa = 10.21 SKK268 pKa = 10.83 AEE270 pKa = 3.78 KK271 pKa = 8.74 TQFIKK276 pKa = 10.61 EE277 pKa = 4.12 SQARR281 pKa = 11.84 KK282 pKa = 8.93 AAQTKK287 pKa = 10.26 NEE289 pKa = 3.73 NLGYY293 pKa = 11.09 GNTKK297 pKa = 8.23 KK298 pKa = 9.4 TNDD301 pKa = 3.35 VLITNKK307 pKa = 9.16 MLQITQKK314 pKa = 10.76 EE315 pKa = 4.25 EE316 pKa = 3.95 QNNKK320 pKa = 9.32
Molecular weight: 37.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.95
IPC2_protein 8.96
IPC_protein 8.814
Toseland 9.663
ProMoST 9.385
Dawson 9.897
Bjellqvist 9.575
Wikipedia 10.043
Rodwell 10.35
Grimsley 9.955
Solomon 9.911
Lehninger 9.882
Nozaki 9.736
DTASelect 9.545
Thurlkill 9.75
EMBOSS 10.072
Sillero 9.823
Patrickios 5.779
IPC_peptide 9.911
IPC2_peptide 8.141
IPC2.peptide.svr19 8.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1715
67
695
245.0
28.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.706 ± 1.157
0.933 ± 0.346
4.606 ± 0.542
7.522 ± 1.25
3.499 ± 0.424
7.172 ± 1.267
1.574 ± 0.33
7.055 ± 1.141
8.047 ± 1.643
6.589 ± 0.453
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.507 ± 0.284
8.921 ± 0.491
2.507 ± 0.59
5.539 ± 0.533
3.965 ± 0.515
4.257 ± 0.371
8.222 ± 0.331
3.499 ± 0.35
1.691 ± 0.127
5.19 ± 0.743
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here