Prevotella oralis ATCC 33269
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2479 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7RQ92|E7RQ92_9BACT Uncharacterized protein OS=Prevotella oralis ATCC 33269 OX=873533 GN=HMPREF0663_11343 PE=4 SV=1
MM1 pKa = 7.19 YY2 pKa = 7.77 WTLEE6 pKa = 4.11 LASKK10 pKa = 11.0 LEE12 pKa = 4.24 DD13 pKa = 4.06 APWPATKK20 pKa = 10.54 EE21 pKa = 3.89 EE22 pKa = 4.86 LIDD25 pKa = 3.48 YY26 pKa = 8.58 AIRR29 pKa = 11.84 SGAPLEE35 pKa = 4.08 VLEE38 pKa = 4.32 NLQEE42 pKa = 4.71 IEE44 pKa = 4.95 DD45 pKa = 4.04 EE46 pKa = 4.42 GDD48 pKa = 3.53 VYY50 pKa = 11.64 DD51 pKa = 5.01 SIEE54 pKa = 5.25 DD55 pKa = 3.27 IWPDD59 pKa = 3.64 YY60 pKa = 7.51 PTKK63 pKa = 10.83 EE64 pKa = 3.75 DD65 pKa = 3.64 FLFNEE70 pKa = 4.91 DD71 pKa = 3.49 EE72 pKa = 4.45 YY73 pKa = 12.0
Molecular weight: 8.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.856
IPC2_protein 3.579
IPC_protein 3.516
Toseland 3.325
ProMoST 3.681
Dawson 3.503
Bjellqvist 3.668
Wikipedia 3.427
Rodwell 3.35
Grimsley 3.249
Solomon 3.465
Lehninger 3.427
Nozaki 3.63
DTASelect 3.77
Thurlkill 3.389
EMBOSS 3.439
Sillero 3.63
Patrickios 1.761
IPC_peptide 3.465
IPC2_peptide 3.605
IPC2.peptide.svr19 3.665
Protein with the highest isoelectric point:
>tr|E7RS71|E7RS71_9BACT X-Pro dipeptidyl-peptidase (S15 family) OS=Prevotella oralis ATCC 33269 OX=873533 GN=HMPREF0663_12139 PE=4 SV=1
MM1 pKa = 7.56 NEE3 pKa = 3.8 TAYY6 pKa = 8.45 DD7 pKa = 3.59 TPLAGKK13 pKa = 9.88 RR14 pKa = 11.84 SEE16 pKa = 3.92 EE17 pKa = 3.69 AAIQKK22 pKa = 8.93 EE23 pKa = 4.1 AAEE26 pKa = 4.46 ALLDD30 pKa = 3.57 IGVSVLLKK38 pKa = 9.85 QIRR41 pKa = 11.84 IPFSRR46 pKa = 11.84 KK47 pKa = 8.07 RR48 pKa = 11.84 LQWRR52 pKa = 11.84 LTMRR56 pKa = 11.84 RR57 pKa = 11.84 PFLSTQIRR65 pKa = 11.84 IARR68 pKa = 11.84 LYY70 pKa = 10.88 LEE72 pKa = 5.67 LGTTCEE78 pKa = 4.32 QLQSMTKK85 pKa = 10.12 DD86 pKa = 3.05 EE87 pKa = 4.45 EE88 pKa = 4.28 MLFLAEE94 pKa = 4.31 HH95 pKa = 6.44 GRR97 pKa = 11.84 DD98 pKa = 2.94 ISRR101 pKa = 11.84 IVALTVCRR109 pKa = 11.84 SPLRR113 pKa = 11.84 AKK115 pKa = 10.75 LFGGITAWVLRR126 pKa = 11.84 NMVEE130 pKa = 3.94 DD131 pKa = 4.29 RR132 pKa = 11.84 YY133 pKa = 10.25 MRR135 pKa = 11.84 AAMTQFVLLLGTEE148 pKa = 4.82 GFTSIIRR155 pKa = 11.84 SAGMTNPMRR164 pKa = 11.84 LRR166 pKa = 11.84 LSRR169 pKa = 11.84 SRR171 pKa = 11.84 KK172 pKa = 9.3 GSS174 pKa = 3.23
Molecular weight: 19.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.516
IPC_protein 10.35
Toseland 10.657
ProMoST 10.379
Dawson 10.745
Bjellqvist 10.467
Wikipedia 10.965
Rodwell 10.877
Grimsley 10.789
Solomon 10.891
Lehninger 10.862
Nozaki 10.657
DTASelect 10.467
Thurlkill 10.657
EMBOSS 11.067
Sillero 10.687
Patrickios 10.613
IPC_peptide 10.891
IPC2_peptide 9.502
IPC2.peptide.svr19 8.697
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2479
0
2479
841153
38
2526
339.3
38.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.284 ± 0.042
1.279 ± 0.021
5.72 ± 0.036
5.841 ± 0.052
4.491 ± 0.036
6.874 ± 0.044
2.105 ± 0.021
7.03 ± 0.052
6.591 ± 0.047
8.732 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.885 ± 0.022
5.203 ± 0.049
3.547 ± 0.026
3.426 ± 0.024
5.013 ± 0.037
5.964 ± 0.039
5.801 ± 0.042
6.479 ± 0.034
1.225 ± 0.02
4.509 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here