Mycobacterium phage Velveteen

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5Y9I6|S5Y9I6_9CAUD Uncharacterized protein OS=Mycobacterium phage Velveteen OX=1340821 GN=38 PE=4 SV=1
MM1 pKa = 7.66GKK3 pKa = 9.8NDD5 pKa = 2.8WTTWRR10 pKa = 11.84NLIEE14 pKa = 4.55TEE16 pKa = 4.11LSQYY20 pKa = 10.4PGEE23 pKa = 4.27TLIHH27 pKa = 4.94VTPDD31 pKa = 3.09EE32 pKa = 4.39SVLDD36 pKa = 3.62VTFYY40 pKa = 11.21AGYY43 pKa = 10.26GSPEE47 pKa = 4.2GPGFTAWTQNRR58 pKa = 11.84VLFSVCYY65 pKa = 10.53DD66 pKa = 3.09GAEE69 pKa = 4.27WVGSAPRR76 pKa = 11.84NPCEE80 pKa = 4.46EE81 pKa = 3.46ISGPVGGCC89 pKa = 3.18

Molecular weight: 9.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5Z476|S5Z476_9CAUD Uncharacterized protein OS=Mycobacterium phage Velveteen OX=1340821 GN=59 PE=4 SV=1
MM1 pKa = 7.57RR2 pKa = 11.84RR3 pKa = 11.84GAKK6 pKa = 9.71LPEE9 pKa = 4.09WVVEE13 pKa = 4.49RR14 pKa = 11.84IVALSWNGWTTGDD27 pKa = 3.08IAKK30 pKa = 10.25EE31 pKa = 4.2VGCSDD36 pKa = 3.33RR37 pKa = 11.84TVSRR41 pKa = 11.84VRR43 pKa = 11.84VRR45 pKa = 11.84HH46 pKa = 5.55GISRR50 pKa = 11.84GEE52 pKa = 3.86AHH54 pKa = 7.28DD55 pKa = 5.3PIPEE59 pKa = 4.15HH60 pKa = 6.98VLAQAARR67 pKa = 11.84LLDD70 pKa = 4.61DD71 pKa = 4.18GASYY75 pKa = 10.87TDD77 pKa = 3.35AAATVGCSRR86 pKa = 11.84TALRR90 pKa = 11.84RR91 pKa = 11.84KK92 pKa = 9.78FPGRR96 pKa = 11.84GWDD99 pKa = 3.23RR100 pKa = 11.84RR101 pKa = 11.84QCAEE105 pKa = 3.42WRR107 pKa = 11.84VIARR111 pKa = 11.84MEE113 pKa = 3.81RR114 pKa = 11.84AIAA117 pKa = 3.76

Molecular weight: 13.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

17351

30

1176

173.5

19.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.668 ± 0.593

1.308 ± 0.192

6.726 ± 0.211

6.08 ± 0.365

2.864 ± 0.197

8.184 ± 0.503

2.15 ± 0.207

4.346 ± 0.179

3.308 ± 0.208

7.671 ± 0.198

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.236 ± 0.12

3.308 ± 0.195

6.011 ± 0.213

3.787 ± 0.189

7.141 ± 0.382

5.769 ± 0.204

6.478 ± 0.318

7.158 ± 0.278

2.184 ± 0.126

2.622 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski