Amino acid dipepetide frequency for Mycobacterium phage Velveteen

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.833AlaAla: 13.833 ± 1.767
1.095AlaCys: 1.095 ± 0.281
7.378AlaAsp: 7.378 ± 0.741
7.493AlaGlu: 7.493 ± 0.864
2.997AlaPhe: 2.997 ± 0.373
8.184AlaGly: 8.184 ± 1.209
2.478AlaHis: 2.478 ± 0.468
4.726AlaIle: 4.726 ± 0.522
4.15AlaLys: 4.15 ± 0.47
8.646AlaLeu: 8.646 ± 0.833
2.594AlaMet: 2.594 ± 0.469
3.516AlaAsn: 3.516 ± 0.64
5.014AlaPro: 5.014 ± 0.598
3.977AlaGln: 3.977 ± 0.549
7.089AlaArg: 7.089 ± 0.786
5.591AlaSer: 5.591 ± 0.672
6.11AlaThr: 6.11 ± 0.657
6.686AlaVal: 6.686 ± 0.694
2.709AlaTrp: 2.709 ± 0.444
2.421AlaTyr: 2.421 ± 0.375
0.0AlaXaa: 0.0 ± 0.0
Cys
0.634CysAla: 0.634 ± 0.234
0.231CysCys: 0.231 ± 0.112
1.21CysAsp: 1.21 ± 0.298
0.98CysGlu: 0.98 ± 0.234
0.288CysPhe: 0.288 ± 0.138
1.729CysGly: 1.729 ± 0.432
0.403CysHis: 0.403 ± 0.153
0.346CysIle: 0.346 ± 0.145
0.519CysLys: 0.519 ± 0.247
0.807CysLeu: 0.807 ± 0.26
0.288CysMet: 0.288 ± 0.13
0.576CysAsn: 0.576 ± 0.178
1.037CysPro: 1.037 ± 0.29
0.461CysGln: 0.461 ± 0.194
1.21CysArg: 1.21 ± 0.425
0.692CysSer: 0.692 ± 0.31
0.749CysThr: 0.749 ± 0.215
0.634CysVal: 0.634 ± 0.193
0.115CysTrp: 0.115 ± 0.081
0.173CysTyr: 0.173 ± 0.096
0.0CysXaa: 0.0 ± 0.0
Asp
7.32AspAla: 7.32 ± 0.657
0.922AspCys: 0.922 ± 0.216
5.187AspAsp: 5.187 ± 0.701
4.438AspGlu: 4.438 ± 0.528
1.441AspPhe: 1.441 ± 0.275
5.821AspGly: 5.821 ± 0.496
1.268AspHis: 1.268 ± 0.247
2.709AspIle: 2.709 ± 0.427
1.441AspLys: 1.441 ± 0.294
6.34AspLeu: 6.34 ± 0.69
0.807AspMet: 0.807 ± 0.218
1.556AspAsn: 1.556 ± 0.272
4.15AspPro: 4.15 ± 0.538
2.478AspGln: 2.478 ± 0.369
5.937AspArg: 5.937 ± 0.538
3.573AspSer: 3.573 ± 0.51
3.862AspThr: 3.862 ± 0.464
4.841AspVal: 4.841 ± 0.535
1.268AspTrp: 1.268 ± 0.263
1.902AspTyr: 1.902 ± 0.329
0.0AspXaa: 0.0 ± 0.0
Glu
6.571GluAla: 6.571 ± 0.67
0.98GluCys: 0.98 ± 0.282
3.228GluAsp: 3.228 ± 0.389
3.055GluGlu: 3.055 ± 0.461
2.19GluPhe: 2.19 ± 0.362
2.767GluGly: 2.767 ± 0.396
1.037GluHis: 1.037 ± 0.275
3.055GluIle: 3.055 ± 0.446
2.363GluLys: 2.363 ± 0.398
5.591GluLeu: 5.591 ± 0.725
1.844GluMet: 1.844 ± 0.315
1.556GluAsn: 1.556 ± 0.293
2.651GluPro: 2.651 ± 0.469
3.17GluGln: 3.17 ± 0.4
5.648GluArg: 5.648 ± 0.649
3.573GluSer: 3.573 ± 0.516
4.265GluThr: 4.265 ± 0.54
4.323GluVal: 4.323 ± 0.602
1.268GluTrp: 1.268 ± 0.261
1.671GluTyr: 1.671 ± 0.335
0.0GluXaa: 0.0 ± 0.0
Phe
2.882PheAla: 2.882 ± 0.466
0.231PheCys: 0.231 ± 0.114
3.112PheAsp: 3.112 ± 0.514
1.153PheGlu: 1.153 ± 0.282
0.519PhePhe: 0.519 ± 0.156
3.285PheGly: 3.285 ± 0.591
0.634PheHis: 0.634 ± 0.218
1.21PheIle: 1.21 ± 0.344
1.037PheLys: 1.037 ± 0.266
2.075PheLeu: 2.075 ± 0.301
1.095PheMet: 1.095 ± 0.281
0.98PheAsn: 0.98 ± 0.263
1.499PhePro: 1.499 ± 0.346
0.865PheGln: 0.865 ± 0.331
1.441PheArg: 1.441 ± 0.302
1.268PheSer: 1.268 ± 0.289
2.19PheThr: 2.19 ± 0.334
1.787PheVal: 1.787 ± 0.296
0.692PheTrp: 0.692 ± 0.213
0.692PheTyr: 0.692 ± 0.244
0.0PheXaa: 0.0 ± 0.0
Gly
8.646GlyAla: 8.646 ± 1.11
1.153GlyCys: 1.153 ± 0.264
5.476GlyAsp: 5.476 ± 0.561
4.323GlyGlu: 4.323 ± 0.54
2.478GlyPhe: 2.478 ± 0.324
8.184GlyGly: 8.184 ± 1.335
1.729GlyHis: 1.729 ± 0.287
4.035GlyIle: 4.035 ± 0.558
2.594GlyLys: 2.594 ± 0.341
5.533GlyLeu: 5.533 ± 0.611
1.614GlyMet: 1.614 ± 0.396
2.651GlyAsn: 2.651 ± 0.397
4.092GlyPro: 4.092 ± 0.613
2.709GlyGln: 2.709 ± 0.594
5.072GlyArg: 5.072 ± 0.69
5.36GlySer: 5.36 ± 0.749
5.706GlyThr: 5.706 ± 0.659
5.764GlyVal: 5.764 ± 0.569
2.248GlyTrp: 2.248 ± 0.352
2.478GlyTyr: 2.478 ± 0.438
0.0GlyXaa: 0.0 ± 0.0
His
1.787HisAla: 1.787 ± 0.29
0.288HisCys: 0.288 ± 0.122
1.21HisAsp: 1.21 ± 0.238
1.21HisGlu: 1.21 ± 0.319
0.519HisPhe: 0.519 ± 0.171
1.671HisGly: 1.671 ± 0.305
0.865HisHis: 0.865 ± 0.212
1.095HisIle: 1.095 ± 0.216
0.922HisLys: 0.922 ± 0.233
1.556HisLeu: 1.556 ± 0.254
0.403HisMet: 0.403 ± 0.161
0.922HisAsn: 0.922 ± 0.219
1.21HisPro: 1.21 ± 0.251
0.807HisGln: 0.807 ± 0.212
1.844HisArg: 1.844 ± 0.337
0.576HisSer: 0.576 ± 0.158
1.499HisThr: 1.499 ± 0.307
1.844HisVal: 1.844 ± 0.399
0.461HisTrp: 0.461 ± 0.156
0.807HisTyr: 0.807 ± 0.221
0.0HisXaa: 0.0 ± 0.0
Ile
4.899IleAla: 4.899 ± 0.691
0.807IleCys: 0.807 ± 0.265
3.516IleAsp: 3.516 ± 0.457
3.862IleGlu: 3.862 ± 0.493
1.037IlePhe: 1.037 ± 0.254
3.516IleGly: 3.516 ± 0.498
1.383IleHis: 1.383 ± 0.289
1.383IleIle: 1.383 ± 0.267
1.037IleLys: 1.037 ± 0.259
2.363IleLeu: 2.363 ± 0.403
0.519IleMet: 0.519 ± 0.144
1.499IleAsn: 1.499 ± 0.307
2.824IlePro: 2.824 ± 0.37
1.614IleGln: 1.614 ± 0.275
2.824IleArg: 2.824 ± 0.459
2.075IleSer: 2.075 ± 0.458
3.516IleThr: 3.516 ± 0.531
3.285IleVal: 3.285 ± 0.476
0.749IleTrp: 0.749 ± 0.198
0.749IleTyr: 0.749 ± 0.187
0.0IleXaa: 0.0 ± 0.0
Lys
3.631LysAla: 3.631 ± 0.547
0.346LysCys: 0.346 ± 0.142
1.96LysAsp: 1.96 ± 0.296
1.153LysGlu: 1.153 ± 0.237
1.21LysPhe: 1.21 ± 0.212
2.709LysGly: 2.709 ± 0.431
0.98LysHis: 0.98 ± 0.227
0.807LysIle: 0.807 ± 0.235
1.326LysLys: 1.326 ± 0.279
2.939LysLeu: 2.939 ± 0.448
0.692LysMet: 0.692 ± 0.173
1.21LysAsn: 1.21 ± 0.206
2.709LysPro: 2.709 ± 0.474
1.326LysGln: 1.326 ± 0.285
1.787LysArg: 1.787 ± 0.332
1.729LysSer: 1.729 ± 0.31
2.651LysThr: 2.651 ± 0.436
2.017LysVal: 2.017 ± 0.354
0.922LysTrp: 0.922 ± 0.315
0.98LysTyr: 0.98 ± 0.243
0.0LysXaa: 0.0 ± 0.0
Leu
8.069LeuAla: 8.069 ± 0.846
0.692LeuCys: 0.692 ± 0.189
5.072LeuAsp: 5.072 ± 0.655
4.38LeuGlu: 4.38 ± 0.563
1.96LeuPhe: 1.96 ± 0.352
6.744LeuGly: 6.744 ± 0.703
0.634LeuHis: 0.634 ± 0.174
3.228LeuIle: 3.228 ± 0.462
2.594LeuLys: 2.594 ± 0.49
4.957LeuLeu: 4.957 ± 0.73
1.671LeuMet: 1.671 ± 0.34
3.055LeuAsn: 3.055 ± 0.456
5.476LeuPro: 5.476 ± 0.681
3.228LeuGln: 3.228 ± 0.472
5.418LeuArg: 5.418 ± 0.655
4.899LeuSer: 4.899 ± 0.548
5.764LeuThr: 5.764 ± 0.658
5.648LeuVal: 5.648 ± 0.61
1.095LeuTrp: 1.095 ± 0.283
2.133LeuTyr: 2.133 ± 0.447
0.0LeuXaa: 0.0 ± 0.0
Met
2.017MetAla: 2.017 ± 0.363
0.461MetCys: 0.461 ± 0.252
1.268MetAsp: 1.268 ± 0.242
1.153MetGlu: 1.153 ± 0.257
0.634MetPhe: 0.634 ± 0.217
1.556MetGly: 1.556 ± 0.272
0.288MetHis: 0.288 ± 0.112
1.037MetIle: 1.037 ± 0.239
0.749MetLys: 0.749 ± 0.245
1.556MetLeu: 1.556 ± 0.223
0.519MetMet: 0.519 ± 0.201
1.095MetAsn: 1.095 ± 0.202
1.326MetPro: 1.326 ± 0.265
0.403MetGln: 0.403 ± 0.135
1.499MetArg: 1.499 ± 0.294
2.651MetSer: 2.651 ± 0.376
2.075MetThr: 2.075 ± 0.294
1.383MetVal: 1.383 ± 0.326
0.288MetTrp: 0.288 ± 0.141
0.403MetTyr: 0.403 ± 0.167
0.0MetXaa: 0.0 ± 0.0
Asn
3.228AsnAla: 3.228 ± 0.416
0.346AsnCys: 0.346 ± 0.145
1.556AsnAsp: 1.556 ± 0.254
1.787AsnGlu: 1.787 ± 0.391
0.519AsnPhe: 0.519 ± 0.225
3.631AsnGly: 3.631 ± 0.534
0.749AsnHis: 0.749 ± 0.161
1.095AsnIle: 1.095 ± 0.434
0.807AsnLys: 0.807 ± 0.211
3.516AsnLeu: 3.516 ± 0.48
0.865AsnMet: 0.865 ± 0.18
2.075AsnAsn: 2.075 ± 0.358
2.536AsnPro: 2.536 ± 0.351
1.21AsnGln: 1.21 ± 0.348
2.305AsnArg: 2.305 ± 0.359
1.441AsnSer: 1.441 ± 0.316
2.248AsnThr: 2.248 ± 0.399
1.844AsnVal: 1.844 ± 0.361
0.634AsnTrp: 0.634 ± 0.198
0.692AsnTyr: 0.692 ± 0.178
0.0AsnXaa: 0.0 ± 0.0
Pro
5.821ProAla: 5.821 ± 0.57
0.922ProCys: 0.922 ± 0.26
4.323ProAsp: 4.323 ± 0.495
4.496ProGlu: 4.496 ± 0.504
2.017ProPhe: 2.017 ± 0.322
6.282ProGly: 6.282 ± 0.658
1.729ProHis: 1.729 ± 0.299
2.133ProIle: 2.133 ± 0.28
2.017ProLys: 2.017 ± 0.411
4.38ProLeu: 4.38 ± 0.591
1.383ProMet: 1.383 ± 0.35
1.614ProAsn: 1.614 ± 0.302
4.092ProPro: 4.092 ± 0.608
2.997ProGln: 2.997 ± 0.418
3.401ProArg: 3.401 ± 0.584
2.536ProSer: 2.536 ± 0.31
2.997ProThr: 2.997 ± 0.397
4.38ProVal: 4.38 ± 0.55
0.922ProTrp: 0.922 ± 0.191
1.671ProTyr: 1.671 ± 0.343
0.0ProXaa: 0.0 ± 0.0
Gln
4.899GlnAla: 4.899 ± 0.622
0.346GlnCys: 0.346 ± 0.171
1.499GlnAsp: 1.499 ± 0.313
1.787GlnGlu: 1.787 ± 0.384
1.095GlnPhe: 1.095 ± 0.203
2.709GlnGly: 2.709 ± 0.483
0.922GlnHis: 0.922 ± 0.25
1.96GlnIle: 1.96 ± 0.342
1.268GlnLys: 1.268 ± 0.22
3.573GlnLeu: 3.573 ± 0.499
0.807GlnMet: 0.807 ± 0.211
1.153GlnAsn: 1.153 ± 0.291
2.536GlnPro: 2.536 ± 0.386
1.614GlnGln: 1.614 ± 0.445
2.882GlnArg: 2.882 ± 0.375
2.421GlnSer: 2.421 ± 0.361
1.441GlnThr: 1.441 ± 0.256
2.709GlnVal: 2.709 ± 0.381
1.095GlnTrp: 1.095 ± 0.206
1.153GlnTyr: 1.153 ± 0.35
0.0GlnXaa: 0.0 ± 0.0
Arg
6.744ArgAla: 6.744 ± 0.619
1.326ArgCys: 1.326 ± 0.378
4.899ArgAsp: 4.899 ± 0.619
5.418ArgGlu: 5.418 ± 0.804
2.709ArgPhe: 2.709 ± 0.421
4.323ArgGly: 4.323 ± 0.541
1.153ArgHis: 1.153 ± 0.259
4.15ArgIle: 4.15 ± 0.523
2.421ArgLys: 2.421 ± 0.423
5.014ArgLeu: 5.014 ± 0.63
2.133ArgMet: 2.133 ± 0.397
2.075ArgAsn: 2.075 ± 0.408
3.746ArgPro: 3.746 ± 0.505
2.363ArgGln: 2.363 ± 0.412
5.591ArgArg: 5.591 ± 0.666
4.553ArgSer: 4.553 ± 0.583
3.746ArgThr: 3.746 ± 0.555
5.13ArgVal: 5.13 ± 0.635
1.671ArgTrp: 1.671 ± 0.376
2.19ArgTyr: 2.19 ± 0.297
0.0ArgXaa: 0.0 ± 0.0
Ser
5.648SerAla: 5.648 ± 1.004
0.576SerCys: 0.576 ± 0.207
3.919SerAsp: 3.919 ± 0.419
2.824SerGlu: 2.824 ± 0.467
2.305SerPhe: 2.305 ± 0.447
4.784SerGly: 4.784 ± 0.527
1.037SerHis: 1.037 ± 0.231
2.709SerIle: 2.709 ± 0.367
2.017SerLys: 2.017 ± 0.396
4.035SerLeu: 4.035 ± 0.526
1.499SerMet: 1.499 ± 0.271
1.787SerAsn: 1.787 ± 0.344
3.862SerPro: 3.862 ± 0.498
2.017SerGln: 2.017 ± 0.364
4.207SerArg: 4.207 ± 0.506
3.804SerSer: 3.804 ± 0.702
3.112SerThr: 3.112 ± 0.393
4.265SerVal: 4.265 ± 0.414
1.556SerTrp: 1.556 ± 0.261
1.729SerTyr: 1.729 ± 0.261
0.0SerXaa: 0.0 ± 0.0
Thr
6.859ThrAla: 6.859 ± 0.757
0.576ThrCys: 0.576 ± 0.186
3.862ThrAsp: 3.862 ± 0.568
3.804ThrGlu: 3.804 ± 0.408
1.268ThrPhe: 1.268 ± 0.24
6.11ThrGly: 6.11 ± 0.593
1.729ThrHis: 1.729 ± 0.262
2.939ThrIle: 2.939 ± 0.443
2.133ThrLys: 2.133 ± 0.316
4.726ThrLeu: 4.726 ± 0.583
1.037ThrMet: 1.037 ± 0.246
2.133ThrAsn: 2.133 ± 0.405
4.553ThrPro: 4.553 ± 0.503
2.133ThrGln: 2.133 ± 0.272
4.035ThrArg: 4.035 ± 0.558
3.573ThrSer: 3.573 ± 0.475
4.438ThrThr: 4.438 ± 0.653
5.764ThrVal: 5.764 ± 0.587
1.153ThrTrp: 1.153 ± 0.265
1.96ThrTyr: 1.96 ± 0.285
0.0ThrXaa: 0.0 ± 0.0
Val
8.242ValAla: 8.242 ± 0.73
1.037ValCys: 1.037 ± 0.297
4.611ValAsp: 4.611 ± 0.496
4.669ValGlu: 4.669 ± 0.429
1.729ValPhe: 1.729 ± 0.312
4.899ValGly: 4.899 ± 0.544
1.671ValHis: 1.671 ± 0.355
2.939ValIle: 2.939 ± 0.412
2.421ValLys: 2.421 ± 0.393
5.418ValLeu: 5.418 ± 0.723
1.268ValMet: 1.268 ± 0.251
2.248ValAsn: 2.248 ± 0.366
4.438ValPro: 4.438 ± 0.41
2.882ValGln: 2.882 ± 0.344
4.841ValArg: 4.841 ± 0.728
4.726ValSer: 4.726 ± 0.613
4.899ValThr: 4.899 ± 0.478
5.706ValVal: 5.706 ± 0.646
1.614ValTrp: 1.614 ± 0.287
1.326ValTyr: 1.326 ± 0.293
0.0ValXaa: 0.0 ± 0.0
Trp
2.421TrpAla: 2.421 ± 0.356
0.288TrpCys: 0.288 ± 0.127
1.499TrpAsp: 1.499 ± 0.275
0.98TrpGlu: 0.98 ± 0.25
0.922TrpPhe: 0.922 ± 0.266
1.095TrpGly: 1.095 ± 0.245
0.461TrpHis: 0.461 ± 0.156
0.807TrpIle: 0.807 ± 0.196
0.692TrpLys: 0.692 ± 0.188
1.729TrpLeu: 1.729 ± 0.412
0.807TrpMet: 0.807 ± 0.229
0.403TrpAsn: 0.403 ± 0.143
0.749TrpPro: 0.749 ± 0.203
0.865TrpGln: 0.865 ± 0.234
2.017TrpArg: 2.017 ± 0.334
1.614TrpSer: 1.614 ± 0.35
1.499TrpThr: 1.499 ± 0.337
1.441TrpVal: 1.441 ± 0.354
0.865TrpTrp: 0.865 ± 0.224
0.692TrpTyr: 0.692 ± 0.21
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.536TyrAla: 2.536 ± 0.384
0.461TyrCys: 0.461 ± 0.183
2.478TyrAsp: 2.478 ± 0.485
1.844TyrGlu: 1.844 ± 0.285
0.807TyrPhe: 0.807 ± 0.241
1.844TyrGly: 1.844 ± 0.323
0.346TyrHis: 0.346 ± 0.148
1.095TyrIle: 1.095 ± 0.272
0.576TyrLys: 0.576 ± 0.207
1.96TyrLeu: 1.96 ± 0.322
0.461TyrMet: 0.461 ± 0.151
0.98TyrAsn: 0.98 ± 0.273
1.614TyrPro: 1.614 ± 0.284
0.749TyrGln: 0.749 ± 0.204
2.363TyrArg: 2.363 ± 0.371
1.037TyrSer: 1.037 ± 0.233
2.017TyrThr: 2.017 ± 0.317
2.133TyrVal: 2.133 ± 0.273
0.519TyrTrp: 0.519 ± 0.157
0.403TyrTyr: 0.403 ± 0.134
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 100 proteins (17351 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski