Sugiyamaella lignohabitans
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5131 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A161HJP2|A0A161HJP2_9ASCO ATP-dependent RNA helicase NAM7 OS=Sugiyamaella lignohabitans OX=796027 GN=NAM7 PE=3 SV=1
MM1 pKa = 7.08 VLAEE5 pKa = 4.17 EE6 pKa = 4.69 VVHH9 pKa = 6.97 FRR11 pKa = 11.84 YY12 pKa = 9.66 HH13 pKa = 4.87 YY14 pKa = 10.34 HH15 pKa = 6.01 LQLPLSHH22 pKa = 7.12 LYY24 pKa = 9.39 IHH26 pKa = 7.02 IFDD29 pKa = 3.66 RR30 pKa = 11.84 HH31 pKa = 6.76 IPDD34 pKa = 3.83 SEE36 pKa = 4.5 TVALDD41 pKa = 3.34 ASVPAEE47 pKa = 3.7 AVVVGIYY54 pKa = 7.39 QTHH57 pKa = 6.74 LAVDD61 pKa = 4.22 TDD63 pKa = 3.96 TLADD67 pKa = 3.82 TLADD71 pKa = 3.88 TLLDD75 pKa = 3.99 TPVDD79 pKa = 3.91 TAADD83 pKa = 4.08 TPVDD87 pKa = 3.8 TSVDD91 pKa = 3.9 TPVDD95 pKa = 3.88 TPVDD99 pKa = 4.09 TPAVDD104 pKa = 3.87 APAVDD109 pKa = 3.84 AAANAAVRR117 pKa = 11.84 TWW119 pKa = 2.82
Molecular weight: 12.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.772
IPC2_protein 3.973
IPC_protein 3.961
Toseland 3.745
ProMoST 4.164
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.961
Rodwell 3.795
Grimsley 3.656
Solomon 3.973
Lehninger 3.935
Nozaki 4.101
DTASelect 4.406
Thurlkill 3.821
EMBOSS 3.961
Sillero 4.101
Patrickios 1.964
IPC_peptide 3.961
IPC2_peptide 4.075
IPC2.peptide.svr19 3.965
Protein with the highest isoelectric point:
>tr|A0A167CHK7|A0A167CHK7_9ASCO Slg1p OS=Sugiyamaella lignohabitans OX=796027 GN=SLG1 PE=4 SV=1
MM1 pKa = 7.7 SYY3 pKa = 11.04 NGIGLKK9 pKa = 7.51 TARR12 pKa = 11.84 GSGTNGYY19 pKa = 7.74 IQKK22 pKa = 10.49 SSATEE27 pKa = 3.83 RR28 pKa = 11.84 FSEE31 pKa = 4.36 GVYY34 pKa = 10.55 AHH36 pKa = 7.0 RR37 pKa = 11.84 QRR39 pKa = 11.84 QQARR43 pKa = 11.84 YY44 pKa = 9.55 EE45 pKa = 3.76 RR46 pKa = 11.84 DD47 pKa = 3.07 QRR49 pKa = 11.84 IWASTNIADD58 pKa = 4.49 RR59 pKa = 11.84 YY60 pKa = 8.23 VDD62 pKa = 3.3 QGIIEE67 pKa = 4.67 HH68 pKa = 6.25 DD69 pKa = 3.62 RR70 pKa = 11.84 KK71 pKa = 10.49 RR72 pKa = 11.84 QVEE75 pKa = 4.49 VKK77 pKa = 10.37 CCEE80 pKa = 4.03 LRR82 pKa = 11.84 DD83 pKa = 3.97 KK84 pKa = 11.41 LEE86 pKa = 4.08 EE87 pKa = 5.03 AGDD90 pKa = 3.6 IAEE93 pKa = 5.36 DD94 pKa = 4.1 EE95 pKa = 4.41 IEE97 pKa = 4.3 KK98 pKa = 10.5 QVSDD102 pKa = 3.8 LRR104 pKa = 11.84 KK105 pKa = 9.51 EE106 pKa = 3.89 LHH108 pKa = 6.63 EE109 pKa = 4.38 KK110 pKa = 10.25 LKK112 pKa = 10.87 KK113 pKa = 10.33 DD114 pKa = 3.47 GQLDD118 pKa = 3.95 GSKK121 pKa = 10.19 LASTHH126 pKa = 5.29 EE127 pKa = 4.14 VAEE130 pKa = 4.48 AKK132 pKa = 10.39 AALNEE137 pKa = 4.0 RR138 pKa = 11.84 LQTAINQASTKK149 pKa = 9.75 KK150 pKa = 10.65 VYY152 pKa = 10.37 GSSSRR157 pKa = 11.84 VRR159 pKa = 11.84 RR160 pKa = 11.84 GEE162 pKa = 3.9 RR163 pKa = 11.84 GDD165 pKa = 4.45 DD166 pKa = 3.19 RR167 pKa = 11.84 SEE169 pKa = 4.11 PAGHH173 pKa = 6.82 RR174 pKa = 11.84 RR175 pKa = 11.84 DD176 pKa = 3.81 GRR178 pKa = 11.84 NGPDD182 pKa = 3.61 DD183 pKa = 4.25 RR184 pKa = 11.84 GHH186 pKa = 6.31 SKK188 pKa = 10.72 RR189 pKa = 11.84 SDD191 pKa = 3.25 RR192 pKa = 11.84 DD193 pKa = 3.19 DD194 pKa = 3.53 DD195 pKa = 3.69 RR196 pKa = 11.84 RR197 pKa = 11.84 AGHH200 pKa = 6.19 SRR202 pKa = 11.84 SQRR205 pKa = 11.84 GGEE208 pKa = 3.41 RR209 pKa = 11.84 WGRR212 pKa = 11.84 EE213 pKa = 3.72 GRR215 pKa = 11.84 EE216 pKa = 4.22 KK217 pKa = 10.37 EE218 pKa = 4.33 WKK220 pKa = 10.23 ADD222 pKa = 3.72 DD223 pKa = 3.74 YY224 pKa = 11.98 DD225 pKa = 4.15 DD226 pKa = 4.19 YY227 pKa = 11.75 KK228 pKa = 11.02 EE229 pKa = 4.09 SRR231 pKa = 11.84 RR232 pKa = 11.84 SYY234 pKa = 10.78 DD235 pKa = 2.69 RR236 pKa = 11.84 RR237 pKa = 11.84 GRR239 pKa = 11.84 SRR241 pKa = 11.84 SQSRR245 pKa = 11.84 SRR247 pKa = 11.84 SPSRR251 pKa = 11.84 SPPASSGRR259 pKa = 11.84 GHH261 pKa = 6.19 SSRR264 pKa = 11.84 RR265 pKa = 11.84 ARR267 pKa = 11.84 SRR269 pKa = 11.84 SPEE272 pKa = 3.38 RR273 pKa = 11.84 RR274 pKa = 11.84 RR275 pKa = 11.84 WSRR278 pKa = 11.84 SPQRR282 pKa = 11.84 RR283 pKa = 11.84 SRR285 pKa = 11.84 RR286 pKa = 11.84 YY287 pKa = 8.13 EE288 pKa = 3.93 SVSRR292 pKa = 11.84 SPPPRR297 pKa = 11.84 SRR299 pKa = 11.84 RR300 pKa = 11.84 RR301 pKa = 11.84 SNSPSKK307 pKa = 8.88 EE308 pKa = 4.19 TRR310 pKa = 11.84 SKK312 pKa = 11.08 SPQNN316 pKa = 3.39
Molecular weight: 36.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.34
IPC2_protein 9.619
IPC_protein 10.584
Toseland 10.76
ProMoST 10.555
Dawson 10.847
Bjellqvist 10.584
Wikipedia 11.096
Rodwell 10.95
Grimsley 10.891
Solomon 11.008
Lehninger 10.965
Nozaki 10.745
DTASelect 10.584
Thurlkill 10.76
EMBOSS 11.184
Sillero 10.774
Patrickios 10.628
IPC_peptide 11.023
IPC2_peptide 9.545
IPC2.peptide.svr19 8.747
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5131
0
5131
2635212
66
5536
513.6
56.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.545 ± 0.046
0.959 ± 0.011
5.877 ± 0.027
6.301 ± 0.047
3.734 ± 0.022
6.425 ± 0.041
2.188 ± 0.018
5.614 ± 0.024
5.609 ± 0.039
8.866 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.809 ± 0.013
4.6 ± 0.02
5.253 ± 0.035
3.97 ± 0.033
5.013 ± 0.03
9.808 ± 0.072
6.011 ± 0.047
6.318 ± 0.029
1.082 ± 0.01
3.018 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here