Methanofollis sp. FWC-SCC2

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia; Methanomicrobiales; Methanomicrobiaceae; Methanofollis; unclassified Methanofollis

Average proteome isoelectric point is 5.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2257 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A483CQC9|A0A483CQC9_9EURY Uncharacterized protein OS=Methanofollis sp. FWC-SCC2 OX=2052832 GN=CUJ86_06325 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 9.44MHH4 pKa = 6.59SVLAAFVLVLFFCQAATASDD24 pKa = 4.01SPEE27 pKa = 3.89VTISADD33 pKa = 3.77PLWSVAGDD41 pKa = 3.53GGSVSLHH48 pKa = 5.42VLVADD53 pKa = 4.83GGAGVNAAAVDD64 pKa = 4.4LSCDD68 pKa = 3.9PSCGTITPAHH78 pKa = 6.78PVTNAHH84 pKa = 5.65GTATATFHH92 pKa = 7.07PSTVAGPVEE101 pKa = 4.27ITASAIWDD109 pKa = 3.97GQDD112 pKa = 3.3VPVTGSVVVHH122 pKa = 6.97IDD124 pKa = 3.06HH125 pKa = 7.95DD126 pKa = 4.16DD127 pKa = 3.98PYY129 pKa = 11.48AITLLEE135 pKa = 4.38FPEE138 pKa = 4.89GATVGSDD145 pKa = 3.08VTITVGIEE153 pKa = 4.0DD154 pKa = 3.94CWGNPIDD161 pKa = 5.28DD162 pKa = 4.63ALDD165 pKa = 3.66VEE167 pKa = 4.96TVNLSVGSPGNAAAFVTDD185 pKa = 4.55GGTGDD190 pKa = 5.87AITLPLDD197 pKa = 3.66SAGQAAATLRR207 pKa = 11.84LDD209 pKa = 3.42TTAGEE214 pKa = 4.42NIVFLDD220 pKa = 3.88APGTVADD227 pKa = 3.92VYY229 pKa = 8.99MTIEE233 pKa = 4.36GVANGTPVSITSAYY247 pKa = 10.5ASASCPANGEE257 pKa = 4.13DD258 pKa = 3.96TVLIAYY264 pKa = 9.56SLLDD268 pKa = 3.58TYY270 pKa = 11.73GNPCSGADD278 pKa = 3.46VRR280 pKa = 11.84IASSLGEE287 pKa = 4.2TATVATNRR295 pKa = 11.84FGSALVRR302 pKa = 11.84YY303 pKa = 8.35GPKK306 pKa = 8.8VTPVDD311 pKa = 3.79VVLTATAVGNGSVTTSDD328 pKa = 3.29TVSFTSTAPVNMALTASLLNMKK350 pKa = 10.18SLDD353 pKa = 3.57VDD355 pKa = 4.01SSSTAAVTARR365 pKa = 11.84ITTEE369 pKa = 3.79GGTPPDD375 pKa = 3.73EE376 pKa = 4.15EE377 pKa = 4.82VEE379 pKa = 4.58VTFSITDD386 pKa = 3.41EE387 pKa = 4.05EE388 pKa = 4.87RR389 pKa = 11.84GGGTAPSLNLATVTTSGGVATATFIPGEE417 pKa = 4.21FSSSDD422 pKa = 3.64PSTGTCTLTATATLNGTPVTRR443 pKa = 11.84SLDD446 pKa = 3.89LAWRR450 pKa = 11.84NYY452 pKa = 9.1PSVSVSVSAEE462 pKa = 3.83PEE464 pKa = 4.05TLVVNNTADD473 pKa = 3.37VTISIRR479 pKa = 11.84GDD481 pKa = 2.85GWEE484 pKa = 4.37FAPPPADD491 pKa = 2.96VMMCISRR498 pKa = 11.84TPTMLRR504 pKa = 11.84GYY506 pKa = 8.77PDD508 pKa = 3.47PMVIAMNASMYY519 pKa = 10.42FAGLMDD525 pKa = 4.81EE526 pKa = 5.64DD527 pKa = 4.32VDD529 pKa = 4.44SLGLVSYY536 pKa = 11.19GEE538 pKa = 4.09EE539 pKa = 4.17TPAGLMADD547 pKa = 3.78LRR549 pKa = 11.84EE550 pKa = 4.23MNSGNTKK557 pKa = 10.07SVYY560 pKa = 10.59NEE562 pKa = 3.39IGYY565 pKa = 10.22DD566 pKa = 3.43PGGGSGAEE574 pKa = 4.09AYY576 pKa = 7.73ITTHH580 pKa = 4.68YY581 pKa = 8.2TKK583 pKa = 10.58SGTVRR588 pKa = 11.84FDD590 pKa = 3.62EE591 pKa = 4.82EE592 pKa = 4.4VTLDD596 pKa = 3.87LPLSTSYY603 pKa = 11.66GSFNDD608 pKa = 3.84SVQCLIPYY616 pKa = 10.11SSLSKK621 pKa = 10.92GQGKK625 pKa = 9.78HH626 pKa = 6.39SGDD629 pKa = 3.82EE630 pKa = 4.03LRR632 pKa = 11.84LALYY636 pKa = 10.38KK637 pKa = 10.73SITEE641 pKa = 3.97IVDD644 pKa = 3.02NGSADD649 pKa = 3.56DD650 pKa = 3.78ARR652 pKa = 11.84AVVVLLDD659 pKa = 4.42DD660 pKa = 4.55FWNNGGGGKK669 pKa = 10.36FGTLYY674 pKa = 10.92GNPLGPDD681 pKa = 3.18GTYY684 pKa = 10.56EE685 pKa = 4.23KK686 pKa = 10.15EE687 pKa = 4.01DD688 pKa = 3.99YY689 pKa = 11.14YY690 pKa = 10.58IFPDD694 pKa = 3.66DD695 pKa = 3.69GRR697 pKa = 11.84GNGHH701 pKa = 5.48MWGSGGRR708 pKa = 11.84WQNLANYY715 pKa = 10.27ASDD718 pKa = 3.56NDD720 pKa = 3.38VRR722 pKa = 11.84IYY724 pKa = 11.22VLLIDD729 pKa = 3.73THH731 pKa = 6.77NGNVNGKK738 pKa = 9.31IGRR741 pKa = 11.84DD742 pKa = 3.38LSTLADD748 pKa = 3.64QTDD751 pKa = 3.48GEE753 pKa = 4.54YY754 pKa = 10.65CYY756 pKa = 11.15VEE758 pKa = 4.94NEE760 pKa = 4.29DD761 pKa = 4.79FEE763 pKa = 6.72SDD765 pKa = 3.86LEE767 pKa = 4.63GFLEE771 pKa = 5.62DD772 pKa = 4.04IKK774 pKa = 11.55DD775 pKa = 3.93DD776 pKa = 3.86LRR778 pKa = 11.84VASVFNTALDD788 pKa = 3.55ASLADD793 pKa = 3.38VTVDD797 pKa = 3.58GEE799 pKa = 4.87SVSSAGVLSYY809 pKa = 10.77EE810 pKa = 4.19YY811 pKa = 11.2VEE813 pKa = 6.11GVSTWITNTSATGDD827 pKa = 3.8DD828 pKa = 4.72LYY830 pKa = 11.48SGTVDD835 pKa = 5.18DD836 pKa = 4.61TGDD839 pKa = 3.37WADD842 pKa = 4.16DD843 pKa = 3.5RR844 pKa = 11.84VLSFGIGTIEE854 pKa = 4.05YY855 pKa = 8.34NQVWTATVRR864 pKa = 11.84LNFSSAGSIGLFTDD878 pKa = 3.56EE879 pKa = 4.88NSVISLSDD887 pKa = 3.52GEE889 pKa = 4.53GNDD892 pKa = 3.81YY893 pKa = 11.1EE894 pKa = 4.76QVLPPLSISVVDD906 pKa = 4.34PDD908 pKa = 5.23DD909 pKa = 3.98VGAFNTTAFDD919 pKa = 3.63VTYY922 pKa = 8.66LTPTGNNEE930 pKa = 3.85TGSIEE935 pKa = 4.3VEE937 pKa = 3.31WGLAYY942 pKa = 10.21EE943 pKa = 4.61GANPEE948 pKa = 4.55SVTQHH953 pKa = 6.52LSYY956 pKa = 10.59QYY958 pKa = 11.27SPDD961 pKa = 3.63NIVWDD966 pKa = 4.15NVWIPVPDD974 pKa = 3.54IPAVYY979 pKa = 9.74EE980 pKa = 4.23GVDD983 pKa = 3.65GVYY986 pKa = 10.59SADD989 pKa = 3.9FSTSGNNGWYY999 pKa = 9.95KK1000 pKa = 9.71IRR1002 pKa = 11.84VRR1004 pKa = 11.84ARR1006 pKa = 11.84EE1007 pKa = 4.28AIDD1010 pKa = 3.64GGAEE1014 pKa = 3.6AWRR1017 pKa = 11.84TADD1020 pKa = 3.62YY1021 pKa = 11.0LVLVNSGNKK1030 pKa = 9.73LVMRR1034 pKa = 11.84ILL1036 pKa = 3.73

Molecular weight: 108.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A483CPG0|A0A483CPG0_9EURY NMD protein affecting ribosome stability and mRNA decay OS=Methanofollis sp. FWC-SCC2 OX=2052832 GN=CUJ86_04500 PE=4 SV=1
MM1 pKa = 7.65SKK3 pKa = 8.86LTKK6 pKa = 9.79GRR8 pKa = 11.84KK9 pKa = 8.52IRR11 pKa = 11.84LAKK14 pKa = 10.47ACMQNRR20 pKa = 11.84RR21 pKa = 11.84VPAWVMIRR29 pKa = 11.84TKK31 pKa = 10.5RR32 pKa = 11.84KK33 pKa = 8.78VASHH37 pKa = 6.72PKK39 pKa = 8.27RR40 pKa = 11.84RR41 pKa = 11.84MWRR44 pKa = 11.84RR45 pKa = 11.84STLKK49 pKa = 10.48VV50 pKa = 3.07

Molecular weight: 6.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2257

0

2257

690178

18

3487

305.8

33.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.824 ± 0.075

1.436 ± 0.03

5.811 ± 0.043

6.977 ± 0.06

3.648 ± 0.035

8.793 ± 0.06

2.016 ± 0.023

6.748 ± 0.063

3.094 ± 0.044

9.091 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.575 ± 0.028

2.785 ± 0.049

4.822 ± 0.035

2.429 ± 0.027

6.649 ± 0.081

5.605 ± 0.046

5.816 ± 0.084

7.938 ± 0.05

1.023 ± 0.022

2.922 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski