Amino acid dipepetide frequency for Methanofollis sp. FWC-SCC2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.135AlaAla: 12.135 ± 0.238
1.297AlaCys: 1.297 ± 0.048
5.948AlaAsp: 5.948 ± 0.096
6.848AlaGlu: 6.848 ± 0.127
3.534AlaPhe: 3.534 ± 0.078
9.722AlaGly: 9.722 ± 0.176
1.773AlaHis: 1.773 ± 0.054
6.443AlaIle: 6.443 ± 0.125
2.26AlaLys: 2.26 ± 0.067
10.213AlaLeu: 10.213 ± 0.185
2.517AlaMet: 2.517 ± 0.065
2.049AlaAsn: 2.049 ± 0.055
3.892AlaPro: 3.892 ± 0.094
1.846AlaGln: 1.846 ± 0.05
5.515AlaArg: 5.515 ± 0.101
4.745AlaSer: 4.745 ± 0.096
4.877AlaThr: 4.877 ± 0.159
9.137AlaVal: 9.137 ± 0.16
1.075AlaTrp: 1.075 ± 0.046
2.42AlaTyr: 2.42 ± 0.059
0.0AlaXaa: 0.0 ± 0.0
Cys
1.447CysAla: 1.447 ± 0.059
0.254CysCys: 0.254 ± 0.02
0.774CysAsp: 0.774 ± 0.031
0.698CysGlu: 0.698 ± 0.03
0.429CysPhe: 0.429 ± 0.027
1.688CysGly: 1.688 ± 0.058
0.34CysHis: 0.34 ± 0.022
0.879CysIle: 0.879 ± 0.036
0.251CysLys: 0.251 ± 0.021
1.036CysLeu: 1.036 ± 0.039
0.352CysMet: 0.352 ± 0.024
0.374CysAsn: 0.374 ± 0.028
0.929CysPro: 0.929 ± 0.044
0.277CysGln: 0.277 ± 0.019
1.136CysArg: 1.136 ± 0.045
0.949CysSer: 0.949 ± 0.04
1.088CysThr: 1.088 ± 0.04
0.869CysVal: 0.869 ± 0.036
0.142CysTrp: 0.142 ± 0.016
0.443CysTyr: 0.443 ± 0.038
0.0CysXaa: 0.0 ± 0.0
Asp
6.039AspAla: 6.039 ± 0.1
0.62AspCys: 0.62 ± 0.034
3.34AspAsp: 3.34 ± 0.078
4.334AspGlu: 4.334 ± 0.093
1.926AspPhe: 1.926 ± 0.067
5.509AspGly: 5.509 ± 0.128
1.217AspHis: 1.217 ± 0.044
3.834AspIle: 3.834 ± 0.07
1.05AspLys: 1.05 ± 0.061
7.124AspLeu: 7.124 ± 0.106
1.203AspMet: 1.203 ± 0.047
1.166AspAsn: 1.166 ± 0.049
3.682AspPro: 3.682 ± 0.079
1.261AspGln: 1.261 ± 0.042
4.086AspArg: 4.086 ± 0.085
2.266AspSer: 2.266 ± 0.071
2.996AspThr: 2.996 ± 0.071
4.158AspVal: 4.158 ± 0.079
0.549AspTrp: 0.549 ± 0.028
1.749AspTyr: 1.749 ± 0.061
0.0AspXaa: 0.0 ± 0.0
Glu
6.336GluAla: 6.336 ± 0.138
0.89GluCys: 0.89 ± 0.038
3.496GluAsp: 3.496 ± 0.079
6.249GluGlu: 6.249 ± 0.136
2.095GluPhe: 2.095 ± 0.066
6.2GluGly: 6.2 ± 0.106
1.53GluHis: 1.53 ± 0.05
5.559GluIle: 5.559 ± 0.102
2.941GluLys: 2.941 ± 0.078
4.186GluLeu: 4.186 ± 0.09
2.212GluMet: 2.212 ± 0.056
2.343GluAsn: 2.343 ± 0.064
2.375GluPro: 2.375 ± 0.06
2.027GluGln: 2.027 ± 0.059
5.939GluArg: 5.939 ± 0.116
3.288GluSer: 3.288 ± 0.078
3.803GluThr: 3.803 ± 0.088
5.504GluVal: 5.504 ± 0.09
0.762GluTrp: 0.762 ± 0.038
2.037GluTyr: 2.037 ± 0.055
0.0GluXaa: 0.0 ± 0.0
Phe
3.343PheAla: 3.343 ± 0.081
0.61PheCys: 0.61 ± 0.027
2.194PheAsp: 2.194 ± 0.064
2.212PheGlu: 2.212 ± 0.076
1.616PhePhe: 1.616 ± 0.054
3.561PheGly: 3.561 ± 0.081
0.693PheHis: 0.693 ± 0.036
2.201PheIle: 2.201 ± 0.075
0.769PheLys: 0.769 ± 0.035
3.34PheLeu: 3.34 ± 0.093
0.793PheMet: 0.793 ± 0.038
0.991PheAsn: 0.991 ± 0.042
1.617PhePro: 1.617 ± 0.052
0.764PheGln: 0.764 ± 0.028
2.202PheArg: 2.202 ± 0.058
2.991PheSer: 2.991 ± 0.08
2.391PheThr: 2.391 ± 0.096
2.766PheVal: 2.766 ± 0.065
0.409PheTrp: 0.409 ± 0.026
1.016PheTyr: 1.016 ± 0.045
0.0PheXaa: 0.0 ± 0.0
Gly
8.06GlyAla: 8.06 ± 0.131
1.517GlyCys: 1.517 ± 0.057
5.174GlyAsp: 5.174 ± 0.091
6.222GlyGlu: 6.222 ± 0.104
3.13GlyPhe: 3.13 ± 0.076
7.718GlyGly: 7.718 ± 0.159
1.507GlyHis: 1.507 ± 0.049
6.643GlyIle: 6.643 ± 0.109
3.196GlyLys: 3.196 ± 0.098
6.692GlyLeu: 6.692 ± 0.125
2.773GlyMet: 2.773 ± 0.071
2.43GlyAsn: 2.43 ± 0.087
2.849GlyPro: 2.849 ± 0.066
1.937GlyGln: 1.937 ± 0.064
6.045GlyArg: 6.045 ± 0.112
5.031GlySer: 5.031 ± 0.108
5.972GlyThr: 5.972 ± 0.186
6.982GlyVal: 6.982 ± 0.1
1.048GlyTrp: 1.048 ± 0.041
3.006GlyTyr: 3.006 ± 0.087
0.0GlyXaa: 0.0 ± 0.0
His
1.998HisAla: 1.998 ± 0.066
0.368HisCys: 0.368 ± 0.023
1.124HisAsp: 1.124 ± 0.039
1.192HisGlu: 1.192 ± 0.046
0.772HisPhe: 0.772 ± 0.043
1.529HisGly: 1.529 ± 0.051
0.559HisHis: 0.559 ± 0.033
1.353HisIle: 1.353 ± 0.051
0.369HisLys: 0.369 ± 0.022
2.339HisLeu: 2.339 ± 0.071
0.38HisMet: 0.38 ± 0.025
0.53HisAsn: 0.53 ± 0.028
1.465HisPro: 1.465 ± 0.046
0.488HisGln: 0.488 ± 0.028
1.413HisArg: 1.413 ± 0.046
0.926HisSer: 0.926 ± 0.043
1.116HisThr: 1.116 ± 0.044
1.361HisVal: 1.361 ± 0.045
0.206HisTrp: 0.206 ± 0.017
0.669HisTyr: 0.669 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
7.163IleAla: 7.163 ± 0.123
0.988IleCys: 0.988 ± 0.039
4.374IleAsp: 4.374 ± 0.083
4.773IleGlu: 4.773 ± 0.1
2.381IlePhe: 2.381 ± 0.072
6.339IleGly: 6.339 ± 0.135
1.274IleHis: 1.274 ± 0.046
3.856IleIle: 3.856 ± 0.101
1.613IleLys: 1.613 ± 0.058
5.785IleLeu: 5.785 ± 0.113
1.345IleMet: 1.345 ± 0.048
1.815IleAsn: 1.815 ± 0.071
3.519IlePro: 3.519 ± 0.072
1.558IleGln: 1.558 ± 0.053
4.839IleArg: 4.839 ± 0.092
3.976IleSer: 3.976 ± 0.077
4.392IleThr: 4.392 ± 0.101
5.128IleVal: 5.128 ± 0.098
0.606IleTrp: 0.606 ± 0.029
1.755IleTyr: 1.755 ± 0.058
0.0IleXaa: 0.0 ± 0.0
Lys
2.479LysAla: 2.479 ± 0.063
0.351LysCys: 0.351 ± 0.022
1.814LysAsp: 1.814 ± 0.07
2.515LysGlu: 2.515 ± 0.076
0.852LysPhe: 0.852 ± 0.048
2.55LysGly: 2.55 ± 0.074
0.59LysHis: 0.59 ± 0.032
2.059LysIle: 2.059 ± 0.069
2.115LysLys: 2.115 ± 0.09
1.846LysLeu: 1.846 ± 0.058
0.978LysMet: 0.978 ± 0.043
1.404LysAsn: 1.404 ± 0.052
1.345LysPro: 1.345 ± 0.055
0.898LysGln: 0.898 ± 0.039
2.202LysArg: 2.202 ± 0.059
1.694LysSer: 1.694 ± 0.056
1.897LysThr: 1.897 ± 0.059
2.104LysVal: 2.104 ± 0.067
0.301LysTrp: 0.301 ± 0.024
0.943LysTyr: 0.943 ± 0.048
0.0LysXaa: 0.0 ± 0.0
Leu
8.695LeuAla: 8.695 ± 0.15
1.339LeuCys: 1.339 ± 0.047
5.164LeuAsp: 5.164 ± 0.085
5.701LeuGlu: 5.701 ± 0.112
3.706LeuPhe: 3.706 ± 0.092
6.635LeuGly: 6.635 ± 0.109
1.844LeuHis: 1.844 ± 0.057
6.2LeuIle: 6.2 ± 0.115
3.28LeuLys: 3.28 ± 0.089
9.215LeuLeu: 9.215 ± 0.179
2.243LeuMet: 2.243 ± 0.067
2.762LeuAsn: 2.762 ± 0.085
4.658LeuPro: 4.658 ± 0.096
2.472LeuGln: 2.472 ± 0.063
5.82LeuArg: 5.82 ± 0.105
6.755LeuSer: 6.755 ± 0.103
4.784LeuThr: 4.784 ± 0.104
6.466LeuVal: 6.466 ± 0.117
0.843LeuTrp: 0.843 ± 0.035
2.327LeuTyr: 2.327 ± 0.062
0.0LeuXaa: 0.0 ± 0.0
Met
2.556MetAla: 2.556 ± 0.074
0.229MetCys: 0.229 ± 0.017
1.582MetAsp: 1.582 ± 0.049
1.892MetGlu: 1.892 ± 0.051
0.651MetPhe: 0.651 ± 0.034
2.108MetGly: 2.108 ± 0.054
0.553MetHis: 0.553 ± 0.031
1.821MetIle: 1.821 ± 0.054
1.321MetLys: 1.321 ± 0.049
2.189MetLeu: 2.189 ± 0.07
0.832MetMet: 0.832 ± 0.041
0.942MetAsn: 0.942 ± 0.042
1.265MetPro: 1.265 ± 0.045
0.777MetGln: 0.777 ± 0.034
1.679MetArg: 1.679 ± 0.053
1.146MetSer: 1.146 ± 0.046
1.584MetThr: 1.584 ± 0.051
1.862MetVal: 1.862 ± 0.051
0.19MetTrp: 0.19 ± 0.018
0.569MetTyr: 0.569 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
2.807AsnAla: 2.807 ± 0.082
0.432AsnCys: 0.432 ± 0.047
1.384AsnAsp: 1.384 ± 0.045
1.633AsnGlu: 1.633 ± 0.062
0.917AsnPhe: 0.917 ± 0.043
2.743AsnGly: 2.743 ± 0.086
0.535AsnHis: 0.535 ± 0.032
1.784AsnIle: 1.784 ± 0.053
0.616AsnLys: 0.616 ± 0.031
2.824AsnLeu: 2.824 ± 0.071
0.626AsnMet: 0.626 ± 0.027
0.917AsnAsn: 0.917 ± 0.05
1.885AsnPro: 1.885 ± 0.057
0.636AsnGln: 0.636 ± 0.037
2.001AsnArg: 2.001 ± 0.049
1.271AsnSer: 1.271 ± 0.07
1.727AsnThr: 1.727 ± 0.074
2.073AsnVal: 2.073 ± 0.08
0.274AsnTrp: 0.274 ± 0.024
0.766AsnTyr: 0.766 ± 0.038
0.0AsnXaa: 0.0 ± 0.0
Pro
5.213ProAla: 5.213 ± 0.113
0.622ProCys: 0.622 ± 0.032
3.554ProAsp: 3.554 ± 0.074
4.079ProGlu: 4.079 ± 0.084
1.949ProPhe: 1.949 ± 0.057
4.297ProGly: 4.297 ± 0.088
0.985ProHis: 0.985 ± 0.039
2.333ProIle: 2.333 ± 0.064
1.233ProLys: 1.233 ± 0.049
4.581ProLeu: 4.581 ± 0.091
1.108ProMet: 1.108 ± 0.04
0.923ProAsn: 0.923 ± 0.035
2.538ProPro: 2.538 ± 0.075
1.072ProGln: 1.072 ± 0.045
2.118ProArg: 2.118 ± 0.066
2.804ProSer: 2.804 ± 0.07
2.353ProThr: 2.353 ± 0.067
4.538ProVal: 4.538 ± 0.087
0.436ProTrp: 0.436 ± 0.026
1.478ProTyr: 1.478 ± 0.05
0.0ProXaa: 0.0 ± 0.0
Gln
2.075GlnAla: 2.075 ± 0.061
0.325GlnCys: 0.325 ± 0.023
1.249GlnAsp: 1.249 ± 0.046
1.934GlnGlu: 1.934 ± 0.059
0.855GlnPhe: 0.855 ± 0.04
1.789GlnGly: 1.789 ± 0.052
0.498GlnHis: 0.498 ± 0.027
1.991GlnIle: 1.991 ± 0.067
1.304GlnLys: 1.304 ± 0.052
1.323GlnLeu: 1.323 ± 0.059
0.851GlnMet: 0.851 ± 0.032
1.023GlnAsn: 1.023 ± 0.037
0.826GlnPro: 0.826 ± 0.031
0.885GlnGln: 0.885 ± 0.049
1.675GlnArg: 1.675 ± 0.055
1.278GlnSer: 1.278 ± 0.05
1.437GlnThr: 1.437 ± 0.051
2.079GlnVal: 2.079 ± 0.059
0.216GlnTrp: 0.216 ± 0.019
0.672GlnTyr: 0.672 ± 0.029
0.0GlnXaa: 0.0 ± 0.0
Arg
5.675ArgAla: 5.675 ± 0.121
1.129ArgCys: 1.129 ± 0.046
3.674ArgAsp: 3.674 ± 0.079
5.319ArgGlu: 5.319 ± 0.112
2.76ArgPhe: 2.76 ± 0.063
4.56ArgGly: 4.56 ± 0.093
1.587ArgHis: 1.587 ± 0.053
5.055ArgIle: 5.055 ± 0.094
2.396ArgLys: 2.396 ± 0.071
5.613ArgLeu: 5.613 ± 0.093
1.913ArgMet: 1.913 ± 0.051
1.794ArgAsn: 1.794 ± 0.051
2.856ArgPro: 2.856 ± 0.067
1.979ArgGln: 1.979 ± 0.061
5.215ArgArg: 5.215 ± 0.106
3.744ArgSer: 3.744 ± 0.077
3.645ArgThr: 3.645 ± 0.077
4.56ArgVal: 4.56 ± 0.098
0.755ArgTrp: 0.755 ± 0.031
2.26ArgTyr: 2.26 ± 0.068
0.0ArgXaa: 0.0 ± 0.0
Ser
5.264SerAla: 5.264 ± 0.102
0.765SerCys: 0.765 ± 0.035
3.234SerAsp: 3.234 ± 0.077
3.179SerGlu: 3.179 ± 0.075
2.198SerPhe: 2.198 ± 0.069
6.161SerGly: 6.161 ± 0.115
1.069SerHis: 1.069 ± 0.037
3.729SerIle: 3.729 ± 0.079
1.417SerLys: 1.417 ± 0.066
5.317SerLeu: 5.317 ± 0.103
1.407SerMet: 1.407 ± 0.048
1.398SerAsn: 1.398 ± 0.059
3.02SerPro: 3.02 ± 0.071
1.13SerGln: 1.13 ± 0.041
3.292SerArg: 3.292 ± 0.065
3.277SerSer: 3.277 ± 0.09
3.127SerThr: 3.127 ± 0.091
4.855SerVal: 4.855 ± 0.101
0.616SerTrp: 0.616 ± 0.033
1.595SerTyr: 1.595 ± 0.053
0.0SerXaa: 0.0 ± 0.0
Thr
6.151ThrAla: 6.151 ± 0.122
0.69ThrCys: 0.69 ± 0.034
3.557ThrAsp: 3.557 ± 0.091
3.431ThrGlu: 3.431 ± 0.109
2.215ThrPhe: 2.215 ± 0.078
5.728ThrGly: 5.728 ± 0.156
1.095ThrHis: 1.095 ± 0.047
3.822ThrIle: 3.822 ± 0.091
1.436ThrLys: 1.436 ± 0.05
5.551ThrLeu: 5.551 ± 0.176
1.226ThrMet: 1.226 ± 0.047
1.492ThrAsn: 1.492 ± 0.097
3.547ThrPro: 3.547 ± 0.09
1.017ThrGln: 1.017 ± 0.038
2.827ThrArg: 2.827 ± 0.07
2.963ThrSer: 2.963 ± 0.074
3.392ThrThr: 3.392 ± 0.099
5.57ThrVal: 5.57 ± 0.141
0.539ThrTrp: 0.539 ± 0.032
1.91ThrTyr: 1.91 ± 0.085
0.0ThrXaa: 0.0 ± 0.0
Val
7.178ValAla: 7.178 ± 0.126
1.298ValCys: 1.298 ± 0.049
4.083ValAsp: 4.083 ± 0.077
5.089ValGlu: 5.089 ± 0.095
3.006ValPhe: 3.006 ± 0.072
5.783ValGly: 5.783 ± 0.104
1.649ValHis: 1.649 ± 0.048
5.465ValIle: 5.465 ± 0.1
2.301ValLys: 2.301 ± 0.061
7.797ValLeu: 7.797 ± 0.143
2.127ValMet: 2.127 ± 0.065
2.115ValAsn: 2.115 ± 0.069
4.206ValPro: 4.206 ± 0.083
2.128ValGln: 2.128 ± 0.06
5.652ValArg: 5.652 ± 0.105
4.657ValSer: 4.657 ± 0.097
5.125ValThr: 5.125 ± 0.153
6.662ValVal: 6.662 ± 0.124
0.808ValTrp: 0.808 ± 0.039
2.253ValTyr: 2.253 ± 0.059
0.0ValXaa: 0.0 ± 0.0
Trp
0.764TrpAla: 0.764 ± 0.035
0.161TrpCys: 0.161 ± 0.016
0.609TrpAsp: 0.609 ± 0.034
0.704TrpGlu: 0.704 ± 0.031
0.39TrpPhe: 0.39 ± 0.027
0.84TrpGly: 0.84 ± 0.045
0.219TrpHis: 0.219 ± 0.018
0.711TrpIle: 0.711 ± 0.035
0.482TrpLys: 0.482 ± 0.031
0.858TrpLeu: 0.858 ± 0.033
0.293TrpMet: 0.293 ± 0.021
0.41TrpAsn: 0.41 ± 0.026
0.275TrpPro: 0.275 ± 0.019
0.368TrpGln: 0.368 ± 0.024
0.697TrpArg: 0.697 ± 0.037
0.606TrpSer: 0.606 ± 0.035
0.606TrpThr: 0.606 ± 0.037
0.707TrpVal: 0.707 ± 0.032
0.188TrpTrp: 0.188 ± 0.02
0.345TrpTyr: 0.345 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.867TyrAla: 2.867 ± 0.085
0.474TyrCys: 0.474 ± 0.025
1.781TyrAsp: 1.781 ± 0.065
1.769TyrGlu: 1.769 ± 0.047
1.095TyrPhe: 1.095 ± 0.046
2.475TyrGly: 2.475 ± 0.062
0.639TyrHis: 0.639 ± 0.031
1.737TyrIle: 1.737 ± 0.058
0.584TyrLys: 0.584 ± 0.034
3.08TyrLeu: 3.08 ± 0.081
0.571TyrMet: 0.571 ± 0.031
0.971TyrAsn: 0.971 ± 0.065
1.468TyrPro: 1.468 ± 0.045
0.765TyrGln: 0.765 ± 0.039
2.136TyrArg: 2.136 ± 0.059
1.687TyrSer: 1.687 ± 0.053
1.846TyrThr: 1.846 ± 0.076
2.001TyrVal: 2.001 ± 0.057
0.269TyrTrp: 0.269 ± 0.021
1.004TyrTyr: 1.004 ± 0.039
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2257 proteins (690178 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski