Phaeodactylibacter luteus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Saprospiria; Saprospirales; Haliscomenobacteraceae; Phaeodactylibacter

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4366 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C6RW63|A0A5C6RW63_9BACT T9SS type B sorting domain-containing protein OS=Phaeodactylibacter luteus OX=1564516 GN=FRY97_05585 PE=4 SV=1
MM1 pKa = 8.01DD2 pKa = 4.93KK3 pKa = 11.16ALATGMLLLHH13 pKa = 6.77SIFLSAQFAPPFDD26 pKa = 4.65LFDD29 pKa = 3.79EE30 pKa = 4.69VGDD33 pKa = 3.8VQFSIGSDD41 pKa = 3.06VDD43 pKa = 3.38GDD45 pKa = 3.55GRR47 pKa = 11.84TDD49 pKa = 3.84VICATAKK56 pKa = 10.38RR57 pKa = 11.84LFWLPSQADD66 pKa = 3.73GTFGHH71 pKa = 5.99QRR73 pKa = 11.84PISFADD79 pKa = 3.07QLYY82 pKa = 10.3RR83 pKa = 11.84SLTAFDD89 pKa = 3.96IDD91 pKa = 4.2ADD93 pKa = 3.92GDD95 pKa = 3.95EE96 pKa = 4.91DD97 pKa = 5.88LLLASYY103 pKa = 11.27NEE105 pKa = 4.76GIGIHH110 pKa = 6.17FNNGDD115 pKa = 3.62GTFGAVVIIYY125 pKa = 10.47SPGPVLDD132 pKa = 5.69LYY134 pKa = 11.45LADD137 pKa = 5.06LNGDD141 pKa = 4.13SLPDD145 pKa = 3.53ALVGQGQGVAWLPGNGDD162 pKa = 3.66GTFGAPSLIAAGHH175 pKa = 6.48DD176 pKa = 3.41AANSVQAEE184 pKa = 4.23DD185 pKa = 4.27LNGDD189 pKa = 3.56GKK191 pKa = 11.31LDD193 pKa = 3.64VVVAAHH199 pKa = 6.46SDD201 pKa = 3.45NRR203 pKa = 11.84LSWHH207 pKa = 6.53EE208 pKa = 3.96NLGDD212 pKa = 3.76GTFADD217 pKa = 3.85YY218 pKa = 11.05QIINEE223 pKa = 4.25THH225 pKa = 7.11LGPNSLDD232 pKa = 3.21IGDD235 pKa = 4.4FDD237 pKa = 5.66GDD239 pKa = 3.49GWPDD243 pKa = 3.32IVCSSRR249 pKa = 11.84NEE251 pKa = 4.1DD252 pKa = 3.81DD253 pKa = 4.28GGAGLALFRR262 pKa = 11.84NLGHH266 pKa = 6.97GSFAAPDD273 pKa = 3.99TLLPAYY279 pKa = 10.27GNYY282 pKa = 9.07FVQAGDD288 pKa = 3.45MDD290 pKa = 4.77GDD292 pKa = 3.98GDD294 pKa = 4.71LDD296 pKa = 3.72LVSCHH301 pKa = 6.99RR302 pKa = 11.84YY303 pKa = 10.57GEE305 pKa = 4.42VGWFQNDD312 pKa = 3.53GTGHH316 pKa = 7.13FGPDD320 pKa = 3.46IKK322 pKa = 11.09LQDD325 pKa = 3.72YY326 pKa = 9.37AAHH329 pKa = 6.23QRR331 pKa = 11.84FLDD334 pKa = 4.15LPDD337 pKa = 4.43LNGDD341 pKa = 3.92GLPDD345 pKa = 3.19ILYY348 pKa = 10.43GINRR352 pKa = 11.84PNPPKK357 pKa = 10.42EE358 pKa = 3.79ALLWLASEE366 pKa = 4.8GGGTFSPPALLEE378 pKa = 4.38LGVSQVSIPVVADD391 pKa = 3.2MDD393 pKa = 3.81QDD395 pKa = 3.52GRR397 pKa = 11.84PDD399 pKa = 3.42VVLGSSGSDD408 pKa = 2.92ALIWVRR414 pKa = 11.84NEE416 pKa = 3.28GHH418 pKa = 6.83GYY420 pKa = 8.39SQPRR424 pKa = 11.84LIARR428 pKa = 11.84RR429 pKa = 11.84HH430 pKa = 5.22NYY432 pKa = 9.06EE433 pKa = 3.85PASIAVFDD441 pKa = 4.64FNNDD445 pKa = 2.5GWPDD449 pKa = 3.48VLAGSVADD457 pKa = 4.24DD458 pKa = 3.83ATNQSMFCVYY468 pKa = 10.67LNNADD473 pKa = 3.57GTFAATPPFEE483 pKa = 4.85SGYY486 pKa = 10.19FYY488 pKa = 11.16KK489 pKa = 10.24RR490 pKa = 11.84DD491 pKa = 3.39LQMADD496 pKa = 3.0MDD498 pKa = 4.66GDD500 pKa = 4.16GDD502 pKa = 6.54LDD504 pKa = 4.08MLWASPGFNGQPTGYY519 pKa = 10.49VGWARR524 pKa = 11.84NNGDD528 pKa = 2.55SWGNFVYY535 pKa = 10.75LLEE538 pKa = 4.43EE539 pKa = 4.12AVGVLDD545 pKa = 5.47VLAADD550 pKa = 4.87LNGDD554 pKa = 3.96GLNDD558 pKa = 3.67LVFCQGEE565 pKa = 4.6SPAIDD570 pKa = 3.22YY571 pKa = 10.34RR572 pKa = 11.84LNQGNGNFVSLQTIANDD589 pKa = 3.33FTTALYY595 pKa = 10.33AQAADD600 pKa = 4.06LNGDD604 pKa = 3.55GLMDD608 pKa = 5.67IIATADD614 pKa = 3.26NSFGGNDD621 pKa = 3.88QIAWWPGTGGGFFGPQQPIYY641 pKa = 10.12IGGPAMEE648 pKa = 5.07KK649 pKa = 10.35FALTDD654 pKa = 3.56YY655 pKa = 11.51NNDD658 pKa = 3.44GEE660 pKa = 4.8LDD662 pKa = 4.03IITSGYY668 pKa = 10.36QDD670 pKa = 5.73FPMQWLEE677 pKa = 3.98GQGGGSFLPPLPIPNAAIAVRR698 pKa = 11.84GFEE701 pKa = 5.58LADD704 pKa = 4.05LDD706 pKa = 4.52NDD708 pKa = 3.77GDD710 pKa = 4.24LDD712 pKa = 3.68IVGNAEE718 pKa = 3.99SPPNGGIDD726 pKa = 3.58RR727 pKa = 11.84AIISYY732 pKa = 10.74NLANDD737 pKa = 3.55VGVSGAVFFDD747 pKa = 3.91EE748 pKa = 4.46NANGLRR754 pKa = 11.84DD755 pKa = 3.62TSEE758 pKa = 4.02YY759 pKa = 11.08LLDD762 pKa = 3.97RR763 pKa = 11.84FPITVAPEE771 pKa = 3.67ALAVFSDD778 pKa = 3.69SEE780 pKa = 4.53GRR782 pKa = 11.84FSVYY786 pKa = 10.1GAEE789 pKa = 4.32GTYY792 pKa = 10.39SISPQLDD799 pKa = 3.77DD800 pKa = 4.18CWSLTTMPGSYY811 pKa = 10.2EE812 pKa = 3.84IVFDD816 pKa = 3.78GTPVDD821 pKa = 3.55TLLFGVSPNTEE832 pKa = 4.01TPEE835 pKa = 4.26GSVTLASAPTRR846 pKa = 11.84CGFTVPFWLNFNNDD860 pKa = 3.37GCWPFDD866 pKa = 3.47GQAFLALNEE875 pKa = 3.97LATYY879 pKa = 10.23IDD881 pKa = 4.59ASPAPSSVSGDD892 pKa = 3.39TLFWSFSALQPGEE905 pKa = 4.13SRR907 pKa = 11.84SINLNMEE914 pKa = 3.78IAGVEE919 pKa = 4.65FIGSALDD926 pKa = 3.76LPSGLVPYY934 pKa = 9.56NAQGEE939 pKa = 4.32ALPAEE944 pKa = 4.56VFAFTSIINCAYY956 pKa = 10.57DD957 pKa = 3.87PNDD960 pKa = 3.82KK961 pKa = 10.25LAHH964 pKa = 6.51PARR967 pKa = 11.84AEE969 pKa = 3.87QPPFSQNYY977 pKa = 7.41TLFDD981 pKa = 3.43EE982 pKa = 4.65PLRR985 pKa = 11.84YY986 pKa = 8.31TIRR989 pKa = 11.84FQNTGTDD996 pKa = 3.12TAFNVVLRR1004 pKa = 11.84DD1005 pKa = 3.67QLSEE1009 pKa = 3.96EE1010 pKa = 4.62LNWEE1014 pKa = 4.05TFEE1017 pKa = 5.12PGASSHH1023 pKa = 6.68PYY1025 pKa = 9.79EE1026 pKa = 4.32ATLYY1030 pKa = 10.93DD1031 pKa = 4.26DD1032 pKa = 4.39GQAAFHH1038 pKa = 6.37FRR1040 pKa = 11.84NILLPDD1046 pKa = 3.41STTNEE1051 pKa = 4.26PGSHH1055 pKa = 6.11GFVQFEE1061 pKa = 4.2IEE1063 pKa = 4.39ALPGLDD1069 pKa = 3.28EE1070 pKa = 4.28NTPIEE1075 pKa = 4.38NTAGIYY1081 pKa = 10.26FDD1083 pKa = 4.95FNPPIITNTVTNLMVSALPDD1103 pKa = 3.74STPVAAFGYY1112 pKa = 9.65EE1113 pKa = 3.99AFSLLVQFNDD1123 pKa = 4.02ASGLSPDD1130 pKa = 2.73SWFWDD1135 pKa = 4.1FGDD1138 pKa = 4.68GGSSTLQHH1146 pKa = 6.97PEE1148 pKa = 3.39HH1149 pKa = 6.89TYY1151 pKa = 11.26ASPGSYY1157 pKa = 9.17EE1158 pKa = 4.14VCLTAANAWGTAQTCQEE1175 pKa = 4.16VTLSSTGTVSVAPVALPEE1193 pKa = 4.23VFPNPARR1200 pKa = 11.84DD1201 pKa = 4.01KK1202 pKa = 11.23VWVKK1206 pKa = 8.67YY1207 pKa = 10.14QPLPSTAPLKK1217 pKa = 10.51IYY1219 pKa = 10.14NAYY1222 pKa = 8.85GQLVLTLPLPSGRR1235 pKa = 11.84SSWPTAGWPAGQYY1248 pKa = 8.51WLQTPSGEE1256 pKa = 3.73RR1257 pKa = 11.84VPFIILKK1264 pKa = 10.22

Molecular weight: 135.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C6RL56|A0A5C6RL56_9BACT Uncharacterized protein OS=Phaeodactylibacter luteus OX=1564516 GN=FRY97_14410 PE=4 SV=1
MM1 pKa = 7.36MGPAPAARR9 pKa = 11.84AGAAMLRR16 pKa = 11.84GSLPARR22 pKa = 11.84PLALQASGLAFGHH35 pKa = 6.72PCAALGQCQDD45 pKa = 4.03IKK47 pKa = 10.12PQKK50 pKa = 9.5HH51 pKa = 3.54KK52 pKa = 10.13HH53 pKa = 4.73LRR55 pKa = 11.84YY56 pKa = 9.47FGSRR60 pKa = 11.84SGKK63 pKa = 9.88ALCC66 pKa = 4.94

Molecular weight: 6.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4366

0

4366

1681301

28

4203

385.1

42.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.532 ± 0.041

0.967 ± 0.023

5.25 ± 0.037

6.55 ± 0.043

4.649 ± 0.026

8.09 ± 0.049

1.902 ± 0.021

5.438 ± 0.03

4.223 ± 0.051

10.261 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.122 ± 0.02

4.026 ± 0.034

4.847 ± 0.034

4.509 ± 0.03

5.114 ± 0.045

6.06 ± 0.033

5.147 ± 0.046

6.238 ± 0.036

1.365 ± 0.015

3.711 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski