Pseudomonas virus phiCTX
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9ZXK6|Q9ZXK6_9CAUD Uncharacterized protein OS=Pseudomonas virus phiCTX OX=35343 PE=4 SV=1
MM1 pKa = 7.63 FFYY4 pKa = 10.72 CPKK7 pKa = 9.9 TGGFYY12 pKa = 10.57 SPEE15 pKa = 3.76 VHH17 pKa = 6.37 GEE19 pKa = 3.84 QMPAEE24 pKa = 4.33 SEE26 pKa = 4.01 LWPLTDD32 pKa = 3.82 EE33 pKa = 4.55 EE34 pKa = 4.99 YY35 pKa = 10.62 EE36 pKa = 4.23 ALLDD40 pKa = 3.94 AQGQGLLIVAGEE52 pKa = 4.34 DD53 pKa = 3.71 GQPVATPPPPLGDD66 pKa = 3.3 EE67 pKa = 4.09 ALATIEE73 pKa = 4.18 RR74 pKa = 11.84 DD75 pKa = 2.97 WRR77 pKa = 11.84 DD78 pKa = 3.28 RR79 pKa = 11.84 QLDD82 pKa = 3.71 DD83 pKa = 3.45 TDD85 pKa = 4.38 ALVARR90 pKa = 11.84 HH91 pKa = 6.18 RR92 pKa = 11.84 DD93 pKa = 3.51 EE94 pKa = 6.39 LEE96 pKa = 3.89 VGTTTLSTEE105 pKa = 4.12 QYY107 pKa = 10.18 QALQAYY113 pKa = 8.69 RR114 pKa = 11.84 RR115 pKa = 11.84 QLRR118 pKa = 11.84 DD119 pKa = 3.06 WPEE122 pKa = 3.95 SGEE125 pKa = 4.22 FPLAEE130 pKa = 4.61 HH131 pKa = 7.0 RR132 pKa = 11.84 PAAPDD137 pKa = 3.0 WLEE140 pKa = 3.87 ALFADD145 pKa = 5.47 GVLL148 pKa = 3.74
Molecular weight: 16.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.232
IPC2_protein 4.113
IPC_protein 4.05
Toseland 3.872
ProMoST 4.177
Dawson 4.012
Bjellqvist 4.164
Wikipedia 3.897
Rodwell 3.884
Grimsley 3.783
Solomon 3.999
Lehninger 3.961
Nozaki 4.126
DTASelect 4.291
Thurlkill 3.897
EMBOSS 3.91
Sillero 4.164
Patrickios 3.49
IPC_peptide 4.012
IPC2_peptide 4.151
IPC2.peptide.svr19 4.083
Protein with the highest isoelectric point:
>tr|Q9ZXL3|Q9ZXL3_9CAUD Uncharacterized protein OS=Pseudomonas virus phiCTX OX=35343 PE=4 SV=1
MM1 pKa = 7.78 ADD3 pKa = 3.3 SLEE6 pKa = 4.28 ALEE9 pKa = 5.45 DD10 pKa = 3.17 WAGPILRR17 pKa = 11.84 ALEE20 pKa = 4.34 PGPRR24 pKa = 11.84 AALARR29 pKa = 11.84 SLARR33 pKa = 11.84 DD34 pKa = 3.62 LRR36 pKa = 11.84 RR37 pKa = 11.84 SQQKK41 pKa = 9.64 RR42 pKa = 11.84 VMAQRR47 pKa = 11.84 NPDD50 pKa = 3.01 GSAYY54 pKa = 9.74 EE55 pKa = 3.92 PRR57 pKa = 11.84 KK58 pKa = 9.92 KK59 pKa = 10.35 RR60 pKa = 11.84 EE61 pKa = 3.81 LRR63 pKa = 11.84 GKK65 pKa = 9.41 QGRR68 pKa = 11.84 IRR70 pKa = 11.84 RR71 pKa = 11.84 KK72 pKa = 9.14 IKK74 pKa = 9.58 MFQKK78 pKa = 10.42 LRR80 pKa = 11.84 TVRR83 pKa = 11.84 YY84 pKa = 9.46 LRR86 pKa = 11.84 AKK88 pKa = 10.49 GDD90 pKa = 3.49 AQAITVSFAGRR101 pKa = 11.84 IARR104 pKa = 11.84 IARR107 pKa = 11.84 VHH109 pKa = 5.85 QYY111 pKa = 10.63 GLRR114 pKa = 11.84 DD115 pKa = 3.44 RR116 pKa = 11.84 AEE118 pKa = 4.16 PGAPEE123 pKa = 3.63 VSYY126 pKa = 10.87 AQRR129 pKa = 11.84 LLLGFDD135 pKa = 3.6 SSDD138 pKa = 3.19 MEE140 pKa = 4.62 TIQNGILAHH149 pKa = 6.84 IDD151 pKa = 3.35 ANSPII156 pKa = 3.85
Molecular weight: 17.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.291
IPC2_protein 9.823
IPC_protein 10.979
Toseland 11.096
ProMoST 11.301
Dawson 11.14
Bjellqvist 10.994
Wikipedia 11.491
Rodwell 11.14
Grimsley 11.184
Solomon 11.462
Lehninger 11.403
Nozaki 11.082
DTASelect 10.994
Thurlkill 11.096
EMBOSS 11.55
Sillero 11.111
Patrickios 10.862
IPC_peptide 11.477
IPC2_peptide 10.292
IPC2.peptide.svr19 8.902
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
47
0
47
10928
35
904
232.5
25.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.365 ± 0.621
1.052 ± 0.145
6.058 ± 0.315
5.344 ± 0.411
3.066 ± 0.301
7.824 ± 0.504
2.004 ± 0.152
4.804 ± 0.257
4.036 ± 0.348
10.505 ± 0.468
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.077 ± 0.209
3.386 ± 0.257
4.621 ± 0.339
4.758 ± 0.362
6.982 ± 0.425
5.756 ± 0.333
5.728 ± 0.32
6.067 ± 0.347
1.757 ± 0.172
2.809 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here