Pseudomonas virus phiCTX

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Citexvirus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q9ZXK6|Q9ZXK6_9CAUD Uncharacterized protein OS=Pseudomonas virus phiCTX OX=35343 PE=4 SV=1
MM1 pKa = 7.63FFYY4 pKa = 10.72CPKK7 pKa = 9.9TGGFYY12 pKa = 10.57SPEE15 pKa = 3.76VHH17 pKa = 6.37GEE19 pKa = 3.84QMPAEE24 pKa = 4.33SEE26 pKa = 4.01LWPLTDD32 pKa = 3.82EE33 pKa = 4.55EE34 pKa = 4.99YY35 pKa = 10.62EE36 pKa = 4.23ALLDD40 pKa = 3.94AQGQGLLIVAGEE52 pKa = 4.34DD53 pKa = 3.71GQPVATPPPPLGDD66 pKa = 3.3EE67 pKa = 4.09ALATIEE73 pKa = 4.18RR74 pKa = 11.84DD75 pKa = 2.97WRR77 pKa = 11.84DD78 pKa = 3.28RR79 pKa = 11.84QLDD82 pKa = 3.71DD83 pKa = 3.45TDD85 pKa = 4.38ALVARR90 pKa = 11.84HH91 pKa = 6.18RR92 pKa = 11.84DD93 pKa = 3.51EE94 pKa = 6.39LEE96 pKa = 3.89VGTTTLSTEE105 pKa = 4.12QYY107 pKa = 10.18QALQAYY113 pKa = 8.69RR114 pKa = 11.84RR115 pKa = 11.84QLRR118 pKa = 11.84DD119 pKa = 3.06WPEE122 pKa = 3.95SGEE125 pKa = 4.22FPLAEE130 pKa = 4.61HH131 pKa = 7.0RR132 pKa = 11.84PAAPDD137 pKa = 3.0WLEE140 pKa = 3.87ALFADD145 pKa = 5.47GVLL148 pKa = 3.74

Molecular weight:
16.61 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q9ZXL3|Q9ZXL3_9CAUD Uncharacterized protein OS=Pseudomonas virus phiCTX OX=35343 PE=4 SV=1
MM1 pKa = 7.78ADD3 pKa = 3.3SLEE6 pKa = 4.28ALEE9 pKa = 5.45DD10 pKa = 3.17WAGPILRR17 pKa = 11.84ALEE20 pKa = 4.34PGPRR24 pKa = 11.84AALARR29 pKa = 11.84SLARR33 pKa = 11.84DD34 pKa = 3.62LRR36 pKa = 11.84RR37 pKa = 11.84SQQKK41 pKa = 9.64RR42 pKa = 11.84VMAQRR47 pKa = 11.84NPDD50 pKa = 3.01GSAYY54 pKa = 9.74EE55 pKa = 3.92PRR57 pKa = 11.84KK58 pKa = 9.92KK59 pKa = 10.35RR60 pKa = 11.84EE61 pKa = 3.81LRR63 pKa = 11.84GKK65 pKa = 9.41QGRR68 pKa = 11.84IRR70 pKa = 11.84RR71 pKa = 11.84KK72 pKa = 9.14IKK74 pKa = 9.58MFQKK78 pKa = 10.42LRR80 pKa = 11.84TVRR83 pKa = 11.84YY84 pKa = 9.46LRR86 pKa = 11.84AKK88 pKa = 10.49GDD90 pKa = 3.49AQAITVSFAGRR101 pKa = 11.84IARR104 pKa = 11.84IARR107 pKa = 11.84VHH109 pKa = 5.85QYY111 pKa = 10.63GLRR114 pKa = 11.84DD115 pKa = 3.44RR116 pKa = 11.84AEE118 pKa = 4.16PGAPEE123 pKa = 3.63VSYY126 pKa = 10.87AQRR129 pKa = 11.84LLLGFDD135 pKa = 3.6SSDD138 pKa = 3.19MEE140 pKa = 4.62TIQNGILAHH149 pKa = 6.84IDD151 pKa = 3.35ANSPII156 pKa = 3.85

Molecular weight:
17.59 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

10928

35

904

232.5

25.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.365 ± 0.621

1.052 ± 0.145

6.058 ± 0.315

5.344 ± 0.411

3.066 ± 0.301

7.824 ± 0.504

2.004 ± 0.152

4.804 ± 0.257

4.036 ± 0.348

10.505 ± 0.468

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.077 ± 0.209

3.386 ± 0.257

4.621 ± 0.339

4.758 ± 0.362

6.982 ± 0.425

5.756 ± 0.333

5.728 ± 0.32

6.067 ± 0.347

1.757 ± 0.172

2.809 ± 0.176

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski