Wuhan house centipede virus 5
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KF14|A0A1L3KF14_9VIRU Capsid protein OS=Wuhan house centipede virus 5 OX=1923709 PE=3 SV=1
MM1 pKa = 7.1 QVFGVPWGFEE11 pKa = 3.86 RR12 pKa = 11.84 EE13 pKa = 3.78 RR14 pKa = 11.84 GYY16 pKa = 10.31 PRR18 pKa = 11.84 MPATTPPSFNTRR30 pKa = 11.84 VQEE33 pKa = 3.81 AFTRR37 pKa = 11.84 IKK39 pKa = 10.97 NEE41 pKa = 3.45 VPIISRR47 pKa = 11.84 YY48 pKa = 9.9 LEE50 pKa = 4.01 EE51 pKa = 4.0 STNYY55 pKa = 9.28 VWPSRR60 pKa = 11.84 QIASEE65 pKa = 4.04 LKK67 pKa = 9.06 SLQVHH72 pKa = 4.98 SEE74 pKa = 4.28 RR75 pKa = 11.84 YY76 pKa = 9.2 FKK78 pKa = 11.18 LSTNAMHH85 pKa = 7.52 PDD87 pKa = 3.66 KK88 pKa = 10.69 DD89 pKa = 3.91 LKK91 pKa = 11.13 SAILYY96 pKa = 9.35 HH97 pKa = 6.78 AEE99 pKa = 3.74 SSYY102 pKa = 11.53 GKK104 pKa = 10.4 ARR106 pKa = 11.84 WTLPEE111 pKa = 5.04 DD112 pKa = 2.89 WWSWRR117 pKa = 11.84 AFKK120 pKa = 10.58 EE121 pKa = 4.02 AVRR124 pKa = 11.84 NLDD127 pKa = 3.5 MTSSPGIPYY136 pKa = 9.59 MKK138 pKa = 10.05 EE139 pKa = 3.41 APTNGKK145 pKa = 6.64 WLKK148 pKa = 9.81 WDD150 pKa = 5.05 GILCDD155 pKa = 3.67 SLQLEE160 pKa = 4.68 RR161 pKa = 11.84 LWHH164 pKa = 5.98 DD165 pKa = 3.46 TQKK168 pKa = 11.01 VIEE171 pKa = 4.51 PEE173 pKa = 3.56 FDD175 pKa = 3.09 LLLRR179 pKa = 11.84 VFIKK183 pKa = 10.43 QEE185 pKa = 3.56 PHH187 pKa = 6.61 KK188 pKa = 10.45 KK189 pKa = 9.97 RR190 pKa = 11.84 KK191 pKa = 9.59 ADD193 pKa = 3.47 EE194 pKa = 4.32 DD195 pKa = 3.26 RR196 pKa = 11.84 WRR198 pKa = 11.84 LIMAAPLSVQVAWQMCFRR216 pKa = 11.84 YY217 pKa = 9.86 MNDD220 pKa = 3.3 LEE222 pKa = 4.26 IEE224 pKa = 4.14 KK225 pKa = 10.56 AYY227 pKa = 10.32 DD228 pKa = 3.47 LPSQQGIILVSGGWKK243 pKa = 10.06 VYY245 pKa = 8.55 SQQWKK250 pKa = 9.07 EE251 pKa = 3.84 QGQTCGMDD259 pKa = 3.17 KK260 pKa = 10.95 SAWDD264 pKa = 3.21 WTAPRR269 pKa = 11.84 WALALDD275 pKa = 3.48 LEE277 pKa = 4.7 FRR279 pKa = 11.84 KK280 pKa = 10.16 RR281 pKa = 11.84 LCNKK285 pKa = 9.26 GMNIAWEE292 pKa = 4.82 HH293 pKa = 5.61 ISEE296 pKa = 4.17 TLYY299 pKa = 11.08 RR300 pKa = 11.84 QMFDD304 pKa = 3.08 NPILVLSDD312 pKa = 3.03 GTAYY316 pKa = 10.31 RR317 pKa = 11.84 QVVPGIMKK325 pKa = 9.98 SGCVNTISTNSHH337 pKa = 5.2 CQVFIHH343 pKa = 6.58 IAVALTYY350 pKa = 9.88 KK351 pKa = 10.32 IPLKK355 pKa = 10.14 PLPRR359 pKa = 11.84 CVGDD363 pKa = 4.28 DD364 pKa = 3.7 TLCTIGQSPGGIISYY379 pKa = 9.92 YY380 pKa = 10.84 DD381 pKa = 3.29 SFGVILKK388 pKa = 10.32 SISDD392 pKa = 3.51 TLEE395 pKa = 3.62 FVGRR399 pKa = 11.84 EE400 pKa = 4.04 FTDD403 pKa = 3.31 EE404 pKa = 4.53 GPIPMYY410 pKa = 10.14 IGKK413 pKa = 8.81 HH414 pKa = 3.84 VVKK417 pKa = 9.79 AQHH420 pKa = 6.27 VRR422 pKa = 11.84 DD423 pKa = 4.39 EE424 pKa = 4.49 DD425 pKa = 3.67 LSMYY429 pKa = 9.43 LDD431 pKa = 3.5 SMARR435 pKa = 11.84 EE436 pKa = 4.39 YY437 pKa = 10.97 CHH439 pKa = 7.09 SPEE442 pKa = 4.01 YY443 pKa = 10.68 FEE445 pKa = 4.05 VWKK448 pKa = 10.79 RR449 pKa = 11.84 LADD452 pKa = 3.77 EE453 pKa = 5.19 LEE455 pKa = 4.4 CSLPLSRR462 pKa = 11.84 EE463 pKa = 4.13 AYY465 pKa = 10.11 LYY467 pKa = 10.08 WYY469 pKa = 8.87 DD470 pKa = 4.02 FPEE473 pKa = 4.48 DD474 pKa = 3.28
Molecular weight: 55.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.711
IPC2_protein 5.753
IPC_protein 5.792
Toseland 6.109
ProMoST 6.02
Dawson 6.033
Bjellqvist 6.059
Wikipedia 6.033
Rodwell 6.02
Grimsley 6.224
Solomon 6.046
Lehninger 6.033
Nozaki 6.3
DTASelect 6.478
Thurlkill 6.478
EMBOSS 6.44
Sillero 6.402
Patrickios 4.024
IPC_peptide 6.059
IPC2_peptide 6.364
IPC2.peptide.svr19 6.387
Protein with the highest isoelectric point:
>tr|A0A1L3KF82|A0A1L3KF82_9VIRU Uncharacterized protein OS=Wuhan house centipede virus 5 OX=1923709 PE=4 SV=1
MM1 pKa = 7.79 ASYY4 pKa = 10.6 CQFSLARR11 pKa = 11.84 VLDD14 pKa = 3.93 MLQAPVLAPEE24 pKa = 4.08 VTGNKK29 pKa = 8.86 WGTLFSGLGKK39 pKa = 9.34 GTLAFVNGTRR49 pKa = 11.84 FGMTCARR56 pKa = 11.84 NTYY59 pKa = 9.35 EE60 pKa = 5.78 ALPDD64 pKa = 3.34 WARR67 pKa = 11.84 QIILCGGVSAASIYY81 pKa = 10.3 AYY83 pKa = 8.63 QRR85 pKa = 11.84 RR86 pKa = 11.84 EE87 pKa = 3.92 RR88 pKa = 11.84 IKK90 pKa = 11.08 DD91 pKa = 3.8 RR92 pKa = 11.84 IALLRR97 pKa = 11.84 TMCKK101 pKa = 9.93 RR102 pKa = 11.84 KK103 pKa = 9.6 IRR105 pKa = 11.84 TIVPTPSSKK114 pKa = 10.96 VYY116 pKa = 9.54 MEE118 pKa = 4.21 SVRR121 pKa = 11.84 KK122 pKa = 9.92 GSEE125 pKa = 3.89 EE126 pKa = 4.42 RR127 pKa = 11.84 PMTSCAAQCTIGRR140 pKa = 11.84 IDD142 pKa = 3.34 VAGDD146 pKa = 3.51 FIVIGNALRR155 pKa = 11.84 FDD157 pKa = 4.45 EE158 pKa = 4.92 DD159 pKa = 3.77 WLVGPDD165 pKa = 3.3 HH166 pKa = 6.91 VLAEE170 pKa = 4.24 DD171 pKa = 3.23 AVYY174 pKa = 10.79 AKK176 pKa = 10.41 GRR178 pKa = 11.84 QSKK181 pKa = 10.25 VSLKK185 pKa = 10.14 GFEE188 pKa = 4.1 RR189 pKa = 11.84 VKK191 pKa = 10.84 LATDD195 pKa = 3.83 LVAIRR200 pKa = 11.84 LKK202 pKa = 10.72 SDD204 pKa = 2.53 DD205 pKa = 3.24 WARR208 pKa = 11.84 IGVAVLKK215 pKa = 10.28 LGHH218 pKa = 5.94 VEE220 pKa = 3.73 GSEE223 pKa = 4.28 FVSICGPAGTGTCGSMSLDD242 pKa = 3.16 HH243 pKa = 7.04 EE244 pKa = 4.51 RR245 pKa = 11.84 FGWTVYY251 pKa = 10.57 EE252 pKa = 4.43 GTTMPGYY259 pKa = 10.57 SGAAYY264 pKa = 10.02 QKK266 pKa = 11.03 GPMAVSMHH274 pKa = 4.52 VHH276 pKa = 6.02 GGVVNAGYY284 pKa = 10.11 AAGYY288 pKa = 9.37 IWVLLKK294 pKa = 10.3 IHH296 pKa = 6.23 MKK298 pKa = 9.91 MYY300 pKa = 10.95 DD301 pKa = 4.0 EE302 pKa = 4.73 DD303 pKa = 4.21 TEE305 pKa = 4.76 SYY307 pKa = 10.72 LRR309 pKa = 11.84 EE310 pKa = 3.6 QHH312 pKa = 6.42 RR313 pKa = 11.84 RR314 pKa = 11.84 GTITRR319 pKa = 11.84 FRR321 pKa = 11.84 SSPWGGDD328 pKa = 3.18 EE329 pKa = 5.62 VIFEE333 pKa = 4.13 INGRR337 pKa = 11.84 FEE339 pKa = 3.74 FHH341 pKa = 7.54 RR342 pKa = 11.84 EE343 pKa = 3.52 RR344 pKa = 11.84 NLAAHH349 pKa = 6.91 FGEE352 pKa = 4.22 NWEE355 pKa = 4.17 QVLRR359 pKa = 11.84 PRR361 pKa = 11.84 GGRR364 pKa = 11.84 QYY366 pKa = 10.88 SRR368 pKa = 11.84 HH369 pKa = 5.46 YY370 pKa = 10.03 RR371 pKa = 11.84 DD372 pKa = 4.17 EE373 pKa = 4.25 VPEE376 pKa = 4.14 GANLVKK382 pKa = 10.7 AKK384 pKa = 10.35 EE385 pKa = 4.12 EE386 pKa = 4.13 PEE388 pKa = 3.77 EE389 pKa = 4.36 SGEE392 pKa = 4.46 CKK394 pKa = 10.37 CSEE397 pKa = 4.33 CLGASNANVVTQGCQQQRR415 pKa = 11.84 LLALTQEE422 pKa = 4.1 YY423 pKa = 10.01 RR424 pKa = 11.84 KK425 pKa = 10.12 LSRR428 pKa = 11.84 ASRR431 pKa = 11.84 TKK433 pKa = 9.93 FRR435 pKa = 11.84 SSLGILKK442 pKa = 8.72 NQPITSGLQDD452 pKa = 3.9 KK453 pKa = 11.02 SPASS457 pKa = 3.53
Molecular weight: 50.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.256
IPC2_protein 8.258
IPC_protein 8.126
Toseland 8.112
ProMoST 8.668
Dawson 8.902
Bjellqvist 9.092
Wikipedia 8.931
Rodwell 8.975
Grimsley 8.097
Solomon 8.99
Lehninger 8.975
Nozaki 9.253
DTASelect 8.873
Thurlkill 8.946
EMBOSS 9.077
Sillero 9.209
Patrickios 4.533
IPC_peptide 8.975
IPC2_peptide 8.053
IPC2.peptide.svr19 8.002
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1425
457
494
475.0
53.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.579 ± 0.865
2.035 ± 0.293
5.123 ± 0.494
6.737 ± 0.242
3.789 ± 0.301
7.158 ± 1.181
1.965 ± 0.255
5.193 ± 0.339
5.193 ± 0.347
7.649 ± 0.531
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.386 ± 0.476
3.298 ± 0.62
5.053 ± 0.627
3.86 ± 0.168
7.018 ± 0.594
7.088 ± 0.194
5.474 ± 0.609
6.807 ± 0.659
2.667 ± 0.619
3.86 ± 0.361
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here