Amino acid dipepetide frequency for Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.877AlaAla: 12.877 ± 0.167
0.699AlaCys: 0.699 ± 0.029
6.237AlaAsp: 6.237 ± 0.092
7.302AlaGlu: 7.302 ± 0.103
3.568AlaPhe: 3.568 ± 0.066
8.767AlaGly: 8.767 ± 0.12
2.118AlaHis: 2.118 ± 0.049
5.858AlaIle: 5.858 ± 0.096
3.616AlaLys: 3.616 ± 0.07
11.085AlaLeu: 11.085 ± 0.127
2.629AlaMet: 2.629 ± 0.061
2.952AlaAsn: 2.952 ± 0.056
4.593AlaPro: 4.593 ± 0.081
4.173AlaGln: 4.173 ± 0.077
5.823AlaArg: 5.823 ± 0.096
6.235AlaSer: 6.235 ± 0.097
6.62AlaThr: 6.62 ± 0.081
8.619AlaVal: 8.619 ± 0.127
1.382AlaTrp: 1.382 ± 0.041
2.076AlaTyr: 2.076 ± 0.049
0.0AlaXaa: 0.0 ± 0.0
Cys
0.756CysAla: 0.756 ± 0.031
0.078CysCys: 0.078 ± 0.01
0.418CysAsp: 0.418 ± 0.021
0.382CysGlu: 0.382 ± 0.022
0.244CysPhe: 0.244 ± 0.016
0.676CysGly: 0.676 ± 0.025
0.159CysHis: 0.159 ± 0.015
0.318CysIle: 0.318 ± 0.018
0.139CysLys: 0.139 ± 0.012
0.585CysLeu: 0.585 ± 0.029
0.13CysMet: 0.13 ± 0.012
0.19CysAsn: 0.19 ± 0.015
0.342CysPro: 0.342 ± 0.021
0.191CysGln: 0.191 ± 0.015
0.327CysArg: 0.327 ± 0.019
0.558CysSer: 0.558 ± 0.026
0.458CysThr: 0.458 ± 0.021
0.512CysVal: 0.512 ± 0.023
0.093CysTrp: 0.093 ± 0.011
0.138CysTyr: 0.138 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
6.56AspAla: 6.56 ± 0.099
0.357AspCys: 0.357 ± 0.02
3.542AspAsp: 3.542 ± 0.078
4.002AspGlu: 4.002 ± 0.066
2.16AspPhe: 2.16 ± 0.049
4.799AspGly: 4.799 ± 0.112
1.285AspHis: 1.285 ± 0.04
3.314AspIle: 3.314 ± 0.062
1.922AspLys: 1.922 ± 0.05
6.019AspLeu: 6.019 ± 0.077
1.256AspMet: 1.256 ± 0.038
1.919AspAsn: 1.919 ± 0.052
3.461AspPro: 3.461 ± 0.063
1.931AspGln: 1.931 ± 0.04
3.281AspArg: 3.281 ± 0.07
3.536AspSer: 3.536 ± 0.07
3.174AspThr: 3.174 ± 0.054
4.694AspVal: 4.694 ± 0.084
0.734AspTrp: 0.734 ± 0.029
1.502AspTyr: 1.502 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
6.567GluAla: 6.567 ± 0.091
0.313GluCys: 0.313 ± 0.018
3.557GluAsp: 3.557 ± 0.074
4.503GluGlu: 4.503 ± 0.092
2.398GluPhe: 2.398 ± 0.055
4.33GluGly: 4.33 ± 0.072
1.552GluHis: 1.552 ± 0.043
3.973GluIle: 3.973 ± 0.074
2.764GluLys: 2.764 ± 0.057
6.844GluLeu: 6.844 ± 0.093
1.301GluMet: 1.301 ± 0.037
2.246GluAsn: 2.246 ± 0.053
2.683GluPro: 2.683 ± 0.064
2.716GluGln: 2.716 ± 0.06
3.632GluArg: 3.632 ± 0.075
3.578GluSer: 3.578 ± 0.068
3.563GluThr: 3.563 ± 0.066
4.799GluVal: 4.799 ± 0.084
0.842GluTrp: 0.842 ± 0.032
1.378GluTyr: 1.378 ± 0.038
0.0GluXaa: 0.0 ± 0.0
Phe
3.685PheAla: 3.685 ± 0.073
0.256PheCys: 0.256 ± 0.018
2.596PheAsp: 2.596 ± 0.052
2.203PheGlu: 2.203 ± 0.051
1.393PhePhe: 1.393 ± 0.047
3.61PheGly: 3.61 ± 0.069
0.795PheHis: 0.795 ± 0.032
1.993PheIle: 1.993 ± 0.051
1.019PheLys: 1.019 ± 0.032
3.299PheLeu: 3.299 ± 0.069
0.847PheMet: 0.847 ± 0.033
1.304PheAsn: 1.304 ± 0.042
1.629PhePro: 1.629 ± 0.045
1.049PheGln: 1.049 ± 0.037
1.7PheArg: 1.7 ± 0.04
2.56PheSer: 2.56 ± 0.051
2.279PheThr: 2.279 ± 0.056
2.679PheVal: 2.679 ± 0.058
0.532PheTrp: 0.532 ± 0.025
0.919PheTyr: 0.919 ± 0.037
0.0PheXaa: 0.0 ± 0.0
Gly
7.953GlyAla: 7.953 ± 0.11
0.621GlyCys: 0.621 ± 0.022
4.208GlyAsp: 4.208 ± 0.072
4.948GlyGlu: 4.948 ± 0.083
3.455GlyPhe: 3.455 ± 0.062
6.31GlyGly: 6.31 ± 0.113
1.786GlyHis: 1.786 ± 0.049
5.035GlyIle: 5.035 ± 0.084
3.029GlyLys: 3.029 ± 0.062
7.839GlyLeu: 7.839 ± 0.11
2.042GlyMet: 2.042 ± 0.049
2.451GlyAsn: 2.451 ± 0.055
2.954GlyPro: 2.954 ± 0.065
2.802GlyGln: 2.802 ± 0.061
4.298GlyArg: 4.298 ± 0.081
5.211GlySer: 5.211 ± 0.077
5.094GlyThr: 5.094 ± 0.074
6.828GlyVal: 6.828 ± 0.097
1.359GlyTrp: 1.359 ± 0.036
2.113GlyTyr: 2.113 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
1.965HisAla: 1.965 ± 0.05
0.174HisCys: 0.174 ± 0.015
1.298HisAsp: 1.298 ± 0.039
1.19HisGlu: 1.19 ± 0.041
0.758HisPhe: 0.758 ± 0.031
1.734HisGly: 1.734 ± 0.048
0.612HisHis: 0.612 ± 0.036
1.179HisIle: 1.179 ± 0.035
0.577HisLys: 0.577 ± 0.027
2.069HisLeu: 2.069 ± 0.051
0.525HisMet: 0.525 ± 0.022
0.738HisAsn: 0.738 ± 0.028
1.369HisPro: 1.369 ± 0.041
0.729HisGln: 0.729 ± 0.03
1.343HisArg: 1.343 ± 0.039
1.31HisSer: 1.31 ± 0.034
1.234HisThr: 1.234 ± 0.034
1.567HisVal: 1.567 ± 0.046
0.284HisTrp: 0.284 ± 0.017
0.546HisTyr: 0.546 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
6.994IleAla: 6.994 ± 0.099
0.406IleCys: 0.406 ± 0.023
4.007IleAsp: 4.007 ± 0.069
3.429IleGlu: 3.429 ± 0.071
1.916IlePhe: 1.916 ± 0.053
4.934IleGly: 4.934 ± 0.083
1.095IleHis: 1.095 ± 0.032
3.302IleIle: 3.302 ± 0.071
1.836IleLys: 1.836 ± 0.037
4.8IleLeu: 4.8 ± 0.077
1.217IleMet: 1.217 ± 0.036
2.147IleAsn: 2.147 ± 0.048
3.096IlePro: 3.096 ± 0.049
1.467IleGln: 1.467 ± 0.044
2.86IleArg: 2.86 ± 0.05
3.892IleSer: 3.892 ± 0.074
3.765IleThr: 3.765 ± 0.078
4.768IleVal: 4.768 ± 0.073
0.584IleTrp: 0.584 ± 0.029
1.231IleTyr: 1.231 ± 0.038
0.0IleXaa: 0.0 ± 0.0
Lys
3.638LysAla: 3.638 ± 0.066
0.152LysCys: 0.152 ± 0.013
2.03LysAsp: 2.03 ± 0.052
2.21LysGlu: 2.21 ± 0.055
1.055LysPhe: 1.055 ± 0.036
2.313LysGly: 2.313 ± 0.056
0.786LysHis: 0.786 ± 0.025
2.007LysIle: 2.007 ± 0.051
1.86LysLys: 1.86 ± 0.059
3.362LysLeu: 3.362 ± 0.062
0.848LysMet: 0.848 ± 0.029
1.416LysAsn: 1.416 ± 0.041
1.765LysPro: 1.765 ± 0.048
1.336LysGln: 1.336 ± 0.038
2.152LysArg: 2.152 ± 0.05
1.999LysSer: 1.999 ± 0.049
2.063LysThr: 2.063 ± 0.045
2.658LysVal: 2.658 ± 0.05
0.462LysTrp: 0.462 ± 0.022
0.754LysTyr: 0.754 ± 0.029
0.0LysXaa: 0.0 ± 0.0
Leu
10.74LeuAla: 10.74 ± 0.115
0.744LeuCys: 0.744 ± 0.03
6.148LeuAsp: 6.148 ± 0.094
5.553LeuGlu: 5.553 ± 0.09
3.221LeuPhe: 3.221 ± 0.067
8.299LeuGly: 8.299 ± 0.122
1.954LeuHis: 1.954 ± 0.048
5.85LeuIle: 5.85 ± 0.097
3.404LeuLys: 3.404 ± 0.063
8.983LeuLeu: 8.983 ± 0.128
2.115LeuMet: 2.115 ± 0.052
3.283LeuAsn: 3.283 ± 0.062
5.026LeuPro: 5.026 ± 0.069
2.685LeuGln: 2.685 ± 0.066
5.617LeuArg: 5.617 ± 0.08
6.77LeuSer: 6.77 ± 0.091
5.763LeuThr: 5.763 ± 0.078
7.579LeuVal: 7.579 ± 0.092
1.198LeuTrp: 1.198 ± 0.039
1.796LeuTyr: 1.796 ± 0.046
0.0LeuXaa: 0.0 ± 0.0
Met
2.601MetAla: 2.601 ± 0.057
0.189MetCys: 0.189 ± 0.017
1.155MetAsp: 1.155 ± 0.032
1.141MetGlu: 1.141 ± 0.038
0.784MetPhe: 0.784 ± 0.032
1.716MetGly: 1.716 ± 0.053
0.453MetHis: 0.453 ± 0.024
1.317MetIle: 1.317 ± 0.04
0.878MetLys: 0.878 ± 0.029
2.326MetLeu: 2.326 ± 0.053
0.563MetMet: 0.563 ± 0.028
0.837MetAsn: 0.837 ± 0.026
1.176MetPro: 1.176 ± 0.035
0.688MetGln: 0.688 ± 0.029
1.354MetArg: 1.354 ± 0.037
1.881MetSer: 1.881 ± 0.047
1.686MetThr: 1.686 ± 0.049
1.724MetVal: 1.724 ± 0.038
0.351MetTrp: 0.351 ± 0.02
0.413MetTyr: 0.413 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.343AsnAla: 3.343 ± 0.058
0.215AsnCys: 0.215 ± 0.014
1.813AsnAsp: 1.813 ± 0.047
1.816AsnGlu: 1.816 ± 0.051
1.195AsnPhe: 1.195 ± 0.037
2.588AsnGly: 2.588 ± 0.058
0.763AsnHis: 0.763 ± 0.032
1.761AsnIle: 1.761 ± 0.041
1.182AsnLys: 1.182 ± 0.039
3.103AsnLeu: 3.103 ± 0.062
0.727AsnMet: 0.727 ± 0.028
1.306AsnAsn: 1.306 ± 0.043
2.312AsnPro: 2.312 ± 0.047
1.189AsnGln: 1.189 ± 0.038
1.903AsnArg: 1.903 ± 0.05
2.106AsnSer: 2.106 ± 0.043
1.929AsnThr: 1.929 ± 0.05
2.312AsnVal: 2.312 ± 0.051
0.512AsnTrp: 0.512 ± 0.022
0.873AsnTyr: 0.873 ± 0.032
0.0AsnXaa: 0.0 ± 0.0
Pro
5.044ProAla: 5.044 ± 0.089
0.231ProCys: 0.231 ± 0.016
3.082ProAsp: 3.082 ± 0.059
4.331ProGlu: 4.331 ± 0.079
1.685ProPhe: 1.685 ± 0.04
4.04ProGly: 4.04 ± 0.074
1.063ProHis: 1.063 ± 0.033
2.437ProIle: 2.437 ± 0.05
1.659ProLys: 1.659 ± 0.04
4.244ProLeu: 4.244 ± 0.077
1.094ProMet: 1.094 ± 0.033
1.587ProAsn: 1.587 ± 0.049
1.629ProPro: 1.629 ± 0.051
1.84ProGln: 1.84 ± 0.051
2.477ProArg: 2.477 ± 0.053
2.957ProSer: 2.957 ± 0.055
3.256ProThr: 3.256 ± 0.068
4.033ProVal: 4.033 ± 0.071
0.683ProTrp: 0.683 ± 0.028
1.05ProTyr: 1.05 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
3.765GlnAla: 3.765 ± 0.082
0.209GlnCys: 0.209 ± 0.016
1.683GlnAsp: 1.683 ± 0.043
2.129GlnGlu: 2.129 ± 0.053
1.122GlnPhe: 1.122 ± 0.035
2.34GlnGly: 2.34 ± 0.052
0.738GlnHis: 0.738 ± 0.028
2.065GlnIle: 2.065 ± 0.047
1.199GlnLys: 1.199 ± 0.039
3.613GlnLeu: 3.613 ± 0.068
0.846GlnMet: 0.846 ± 0.024
1.042GlnAsn: 1.042 ± 0.035
1.611GlnPro: 1.611 ± 0.05
1.6GlnGln: 1.6 ± 0.048
2.294GlnArg: 2.294 ± 0.059
1.801GlnSer: 1.801 ± 0.046
1.682GlnThr: 1.682 ± 0.048
2.767GlnVal: 2.767 ± 0.058
0.597GlnTrp: 0.597 ± 0.025
0.724GlnTyr: 0.724 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
5.399ArgAla: 5.399 ± 0.094
0.335ArgCys: 0.335 ± 0.018
3.275ArgAsp: 3.275 ± 0.073
3.772ArgGlu: 3.772 ± 0.072
2.221ArgPhe: 2.221 ± 0.054
4.013ArgGly: 4.013 ± 0.074
1.187ArgHis: 1.187 ± 0.039
3.42ArgIle: 3.42 ± 0.055
2.305ArgLys: 2.305 ± 0.055
5.073ArgLeu: 5.073 ± 0.091
1.542ArgMet: 1.542 ± 0.044
1.889ArgAsn: 1.889 ± 0.05
2.489ArgPro: 2.489 ± 0.06
1.967ArgGln: 1.967 ± 0.048
3.979ArgArg: 3.979 ± 0.083
3.382ArgSer: 3.382 ± 0.071
3.396ArgThr: 3.396 ± 0.057
4.043ArgVal: 4.043 ± 0.078
0.847ArgTrp: 0.847 ± 0.03
1.428ArgTyr: 1.428 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
6.561SerAla: 6.561 ± 0.084
0.403SerCys: 0.403 ± 0.021
3.618SerAsp: 3.618 ± 0.055
3.778SerGlu: 3.778 ± 0.068
2.41SerPhe: 2.41 ± 0.047
5.627SerGly: 5.627 ± 0.084
1.261SerHis: 1.261 ± 0.038
3.639SerIle: 3.639 ± 0.071
2.113SerLys: 2.113 ± 0.05
5.932SerLeu: 5.932 ± 0.079
1.692SerMet: 1.692 ± 0.044
2.077SerAsn: 2.077 ± 0.05
3.018SerPro: 3.018 ± 0.059
2.075SerGln: 2.075 ± 0.051
3.513SerArg: 3.513 ± 0.065
4.485SerSer: 4.485 ± 0.086
4.416SerThr: 4.416 ± 0.079
4.826SerVal: 4.826 ± 0.077
1.014SerTrp: 1.014 ± 0.034
1.469SerTyr: 1.469 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
6.403ThrAla: 6.403 ± 0.09
0.416ThrCys: 0.416 ± 0.023
3.43ThrAsp: 3.43 ± 0.065
3.453ThrGlu: 3.453 ± 0.064
2.244ThrPhe: 2.244 ± 0.053
5.181ThrGly: 5.181 ± 0.083
1.344ThrHis: 1.344 ± 0.041
3.542ThrIle: 3.542 ± 0.065
1.847ThrLys: 1.847 ± 0.046
5.809ThrLeu: 5.809 ± 0.089
1.281ThrMet: 1.281 ± 0.038
1.88ThrAsn: 1.88 ± 0.049
3.849ThrPro: 3.849 ± 0.069
1.886ThrGln: 1.886 ± 0.048
3.074ThrArg: 3.074 ± 0.059
4.057ThrSer: 4.057 ± 0.065
4.258ThrThr: 4.258 ± 0.077
5.347ThrVal: 5.347 ± 0.085
0.987ThrTrp: 0.987 ± 0.035
1.435ThrTyr: 1.435 ± 0.042
0.0ThrXaa: 0.0 ± 0.0
Val
8.847ValAla: 8.847 ± 0.104
0.58ValCys: 0.58 ± 0.026
5.279ValAsp: 5.279 ± 0.091
5.336ValGlu: 5.336 ± 0.084
2.957ValPhe: 2.957 ± 0.062
6.115ValGly: 6.115 ± 0.092
1.547ValHis: 1.547 ± 0.038
4.768ValIle: 4.768 ± 0.081
2.391ValLys: 2.391 ± 0.051
7.877ValLeu: 7.877 ± 0.11
1.776ValMet: 1.776 ± 0.05
2.453ValAsn: 2.453 ± 0.05
3.647ValPro: 3.647 ± 0.056
2.139ValGln: 2.139 ± 0.046
4.077ValArg: 4.077 ± 0.079
5.2ValSer: 5.2 ± 0.08
4.957ValThr: 4.957 ± 0.072
7.057ValVal: 7.057 ± 0.093
0.967ValTrp: 0.967 ± 0.032
1.542ValTyr: 1.542 ± 0.04
0.0ValXaa: 0.0 ± 0.0
Trp
1.379TrpAla: 1.379 ± 0.04
0.128TrpCys: 0.128 ± 0.012
0.739TrpAsp: 0.739 ± 0.029
0.771TrpGlu: 0.771 ± 0.027
0.594TrpPhe: 0.594 ± 0.025
1.007TrpGly: 1.007 ± 0.038
0.297TrpHis: 0.297 ± 0.016
0.897TrpIle: 0.897 ± 0.031
0.477TrpLys: 0.477 ± 0.024
1.541TrpLeu: 1.541 ± 0.048
0.382TrpMet: 0.382 ± 0.02
0.501TrpAsn: 0.501 ± 0.023
0.596TrpPro: 0.596 ± 0.027
0.52TrpGln: 0.52 ± 0.023
0.837TrpArg: 0.837 ± 0.028
0.874TrpSer: 0.874 ± 0.03
0.818TrpThr: 0.818 ± 0.035
1.137TrpVal: 1.137 ± 0.039
0.323TrpTrp: 0.323 ± 0.02
0.288TrpTyr: 0.288 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.15TyrAla: 2.15 ± 0.048
0.19TyrCys: 0.19 ± 0.014
1.332TyrAsp: 1.332 ± 0.037
1.287TyrGlu: 1.287 ± 0.043
0.968TyrPhe: 0.968 ± 0.029
1.941TyrGly: 1.941 ± 0.047
0.405TyrHis: 0.405 ± 0.022
1.071TyrIle: 1.071 ± 0.033
0.651TyrLys: 0.651 ± 0.029
2.323TyrLeu: 2.323 ± 0.053
0.416TyrMet: 0.416 ± 0.02
0.732TyrAsn: 0.732 ± 0.028
1.179TyrPro: 1.179 ± 0.036
0.842TyrGln: 0.842 ± 0.029
1.423TyrArg: 1.423 ± 0.044
1.536TyrSer: 1.536 ± 0.039
1.312TyrThr: 1.312 ± 0.038
1.562TyrVal: 1.562 ± 0.043
0.353TyrTrp: 0.353 ± 0.02
0.528TyrTyr: 0.528 ± 0.023
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3094 proteins (958486 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski