Schaedlerella arabinosiphila
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5980 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|N2A3L7|N2A3L7_9FIRM Ethanolamine utilization protein EutN OS=Schaedlerella arabinosiphila OX=2044587 GN=C824_05627 PE=4 SV=1
MM1 pKa = 7.5 KK2 pKa = 10.51 KK3 pKa = 10.05 FVALFLALTLTCGLAACGGGGNSGGGDD30 pKa = 3.74 SNDD33 pKa = 3.71 TGGSGASTEE42 pKa = 4.07 EE43 pKa = 4.17 STDD46 pKa = 3.29 GDD48 pKa = 3.89 EE49 pKa = 5.73 KK50 pKa = 11.07 EE51 pKa = 4.76 DD52 pKa = 3.58 SGSGEE57 pKa = 3.69 KK58 pKa = 10.46 VAINVIAAQYY68 pKa = 8.87 GQNTTKK74 pKa = 9.08 WWSDD78 pKa = 3.22 FVKK81 pKa = 10.58 DD82 pKa = 4.04 FTTEE86 pKa = 3.87 YY87 pKa = 10.42 PNIDD91 pKa = 3.3 LTVEE95 pKa = 3.93 VEE97 pKa = 4.12 SWNDD101 pKa = 2.46 IYY103 pKa = 11.3 TVVNTRR109 pKa = 11.84 ISNNDD114 pKa = 3.06 APDD117 pKa = 3.41 ILNIDD122 pKa = 3.48 VFANYY127 pKa = 9.66 QADD130 pKa = 4.2 DD131 pKa = 4.26 LLLPAEE137 pKa = 4.98 DD138 pKa = 3.96 FVSDD142 pKa = 3.4 EE143 pKa = 4.92 TYY145 pKa = 11.32 KK146 pKa = 11.09 KK147 pKa = 9.54 MYY149 pKa = 9.48 PAFLEE154 pKa = 3.91 QSNVDD159 pKa = 3.41 GTIWAIPDD167 pKa = 3.61 LASARR172 pKa = 11.84 ALYY175 pKa = 10.02 VNKK178 pKa = 10.43 DD179 pKa = 3.03 ILDD182 pKa = 3.93 AAKK185 pKa = 10.86 VEE187 pKa = 5.25 IPTTWDD193 pKa = 3.31 EE194 pKa = 4.22 LKK196 pKa = 10.56 KK197 pKa = 10.76 ACEE200 pKa = 4.18 AIKK203 pKa = 10.5 AYY205 pKa = 10.15 DD206 pKa = 3.53 SKK208 pKa = 10.89 IYY210 pKa = 9.79 PWGIDD215 pKa = 3.14 MTTDD219 pKa = 2.85 EE220 pKa = 4.57 GQAAFAYY227 pKa = 7.2 YY228 pKa = 8.91 TWNNGGGFVDD238 pKa = 5.26 EE239 pKa = 4.97 DD240 pKa = 4.21 NNWALNSDD248 pKa = 4.09 EE249 pKa = 4.56 NVEE252 pKa = 4.03 AVEE255 pKa = 4.26 FAISLVKK262 pKa = 10.53 DD263 pKa = 4.56 GYY265 pKa = 9.47 TNSDD269 pKa = 3.39 PSNDD273 pKa = 3.13 TRR275 pKa = 11.84 YY276 pKa = 10.71 DD277 pKa = 3.61 LQDD280 pKa = 3.21 MFGAGQVAMMIGPNSIPTYY299 pKa = 9.99 IADD302 pKa = 3.56 GGYY305 pKa = 9.63 SVNYY309 pKa = 8.79 EE310 pKa = 4.03 VAPIPTNGDD319 pKa = 3.48 NEE321 pKa = 4.65 SVSAGVMDD329 pKa = 5.53 RR330 pKa = 11.84 FMCFDD335 pKa = 3.57 NGYY338 pKa = 10.62 SDD340 pKa = 5.76 AEE342 pKa = 4.16 LEE344 pKa = 4.39 AIKK347 pKa = 9.97 TFFDD351 pKa = 3.63 YY352 pKa = 10.25 FYY354 pKa = 10.87 EE355 pKa = 4.14 DD356 pKa = 2.68 TRR358 pKa = 11.84 YY359 pKa = 10.59 SDD361 pKa = 3.26 WVLMEE366 pKa = 4.43 GFLPATSTGGEE377 pKa = 3.91 ILAEE381 pKa = 4.46 ADD383 pKa = 3.07 EE384 pKa = 5.56 SMASWVDD391 pKa = 3.24 IVGSCNFYY399 pKa = 9.29 PTAKK403 pKa = 10.06 TEE405 pKa = 3.99 WDD407 pKa = 3.59 DD408 pKa = 3.73 VKK410 pKa = 11.15 QGVINVEE417 pKa = 3.95 QQALLDD423 pKa = 4.22 GNVKK427 pKa = 10.19 EE428 pKa = 5.75 LLDD431 pKa = 3.83 EE432 pKa = 4.48 LQAEE436 pKa = 4.41 IAEE439 pKa = 4.32
Molecular weight: 48.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.695
IPC2_protein 3.668
IPC_protein 3.706
Toseland 3.478
ProMoST 3.846
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.617
Rodwell 3.528
Grimsley 3.389
Solomon 3.681
Lehninger 3.643
Nozaki 3.795
DTASelect 4.037
Thurlkill 3.528
EMBOSS 3.63
Sillero 3.821
Patrickios 1.354
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.738
Protein with the highest isoelectric point:
>tr|N1ZVG7|N1ZVG7_9FIRM HTH cro/C1-type domain-containing protein OS=Schaedlerella arabinosiphila OX=2044587 GN=C824_05465 PE=4 SV=1
MM1 pKa = 7.17 FVCNNRR7 pKa = 11.84 GLSFGIAFYY16 pKa = 10.47 LYY18 pKa = 9.6 HH19 pKa = 6.79 FCLIHH24 pKa = 6.89 SDD26 pKa = 3.3 VRR28 pKa = 11.84 ISRR31 pKa = 11.84 RR32 pKa = 11.84 IGKK35 pKa = 8.94 SRR37 pKa = 11.84 NGVLILMWLWIRR49 pKa = 11.84 FPLLKK54 pKa = 10.51 AVFQGLRR61 pKa = 11.84 AYY63 pKa = 10.25 LRR65 pKa = 11.84 QYY67 pKa = 9.37 QTDD70 pKa = 3.34 QDD72 pKa = 3.39 KK73 pKa = 11.21 KK74 pKa = 10.76 MRR76 pKa = 11.84 DD77 pKa = 2.97 RR78 pKa = 11.84 WTDD81 pKa = 2.65 II82 pKa = 3.42
Molecular weight: 9.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.809
IPC_protein 10.643
Toseland 10.511
ProMoST 10.292
Dawson 10.672
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.847
Grimsley 10.745
Solomon 10.774
Lehninger 10.73
Nozaki 10.526
DTASelect 10.409
Thurlkill 10.555
EMBOSS 10.921
Sillero 10.599
Patrickios 10.599
IPC_peptide 10.76
IPC2_peptide 9.575
IPC2.peptide.svr19 8.353
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5980
0
5980
1817401
21
3066
303.9
34.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.538 ± 0.04
1.553 ± 0.014
5.486 ± 0.025
8.026 ± 0.037
4.165 ± 0.025
7.152 ± 0.038
1.804 ± 0.014
6.978 ± 0.034
6.477 ± 0.031
9.15 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.126 ± 0.018
4.089 ± 0.022
3.37 ± 0.017
3.454 ± 0.017
5.127 ± 0.029
5.818 ± 0.032
5.063 ± 0.022
6.429 ± 0.026
0.998 ± 0.012
4.197 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here