Changjiang tombus-like virus 21

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFQ5|A0A1L3KFQ5_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 21 OX=1922815 PE=4 SV=1
MM1 pKa = 7.52EE2 pKa = 4.69NKK4 pKa = 9.92IYY6 pKa = 10.13QLPEE10 pKa = 4.32FIKK13 pKa = 10.44HH14 pKa = 4.74VPVANRR20 pKa = 11.84ADD22 pKa = 3.8YY23 pKa = 10.43IYY25 pKa = 11.13DD26 pKa = 3.56KK27 pKa = 10.87LYY29 pKa = 9.54KK30 pKa = 9.34TGSRR34 pKa = 11.84YY35 pKa = 10.2SVTDD39 pKa = 3.39HH40 pKa = 6.45SQFEE44 pKa = 4.11AHH46 pKa = 5.72FTRR49 pKa = 11.84NIMEE53 pKa = 4.9HH54 pKa = 7.33IEE56 pKa = 4.19FVVYY60 pKa = 10.16RR61 pKa = 11.84YY62 pKa = 10.23LIQNLPNKK70 pKa = 9.76EE71 pKa = 4.45DD72 pKa = 3.55YY73 pKa = 9.94EE74 pKa = 4.27QLFKK78 pKa = 11.22VLQGKK83 pKa = 7.96NKK85 pKa = 9.11CCFKK89 pKa = 10.32KK90 pKa = 10.48GHH92 pKa = 6.45YY93 pKa = 9.07IVNATRR99 pKa = 11.84MSGEE103 pKa = 4.2MNTSLGNGLTNLLLMLYY120 pKa = 10.08VLNQNGNTNVSGVVEE135 pKa = 4.31GDD137 pKa = 3.66DD138 pKa = 3.91GLFRR142 pKa = 11.84YY143 pKa = 9.67DD144 pKa = 4.92GPDD147 pKa = 3.15PTKK150 pKa = 11.51DD151 pKa = 3.03MFAKK155 pKa = 10.02MGFTVKK161 pKa = 10.9LEE163 pKa = 3.86IFDD166 pKa = 4.43NLNEE170 pKa = 4.06ASFCGLVFDD179 pKa = 4.75IDD181 pKa = 3.38ARR183 pKa = 11.84QIIINPIKK191 pKa = 10.67VLLNFGWLHH200 pKa = 5.0YY201 pKa = 9.67QYY203 pKa = 11.32KK204 pKa = 9.76DD205 pKa = 3.19AKK207 pKa = 10.16FEE209 pKa = 4.62TIMGLLRR216 pKa = 11.84MKK218 pKa = 10.39SLSLLYY224 pKa = 9.85QYY226 pKa = 9.11PSCPIVSEE234 pKa = 3.88LALYY238 pKa = 9.45GLRR241 pKa = 11.84VTSKK245 pKa = 8.4YY246 pKa = 8.93TPVLIRR252 pKa = 11.84EE253 pKa = 4.17NNYY256 pKa = 9.12KK257 pKa = 10.36RR258 pKa = 11.84KK259 pKa = 9.04FHH261 pKa = 6.63EE262 pKa = 4.38EE263 pKa = 3.68VLRR266 pKa = 11.84TPRR269 pKa = 11.84LVADD273 pKa = 4.47MINFKK278 pKa = 9.79TRR280 pKa = 11.84LVCEE284 pKa = 4.16KK285 pKa = 10.28VFKK288 pKa = 10.07ITIDD292 pKa = 3.56DD293 pKa = 3.67QLSIEE298 pKa = 4.69SYY300 pKa = 10.64LKK302 pKa = 10.39QLDD305 pKa = 4.04EE306 pKa = 5.47IKK308 pKa = 10.44PLWHH312 pKa = 6.79LSFHH316 pKa = 6.68TYY318 pKa = 8.58MNQYY322 pKa = 9.57QDD324 pKa = 4.19VYY326 pKa = 9.27LQYY329 pKa = 10.65VGEE332 pKa = 4.27YY333 pKa = 8.98AAGYY337 pKa = 7.46HH338 pKa = 4.91TVVPHH343 pKa = 6.38KK344 pKa = 9.63MVRR347 pKa = 11.84RR348 pKa = 11.84NRR350 pKa = 11.84TKK352 pKa = 9.35QQKK355 pKa = 9.22KK356 pKa = 10.23RR357 pKa = 11.84NNSNNNNNNNNNSKK371 pKa = 10.23KK372 pKa = 10.35KK373 pKa = 9.13EE374 pKa = 3.99IVVVRR379 pKa = 11.84QNNPKK384 pKa = 6.94QTKK387 pKa = 10.39SIGQQIGSALGGLIGHH403 pKa = 6.89GAHH406 pKa = 6.22MLFKK410 pKa = 10.61NITGFGDD417 pKa = 3.62YY418 pKa = 10.75NINNNTLLQGGMTPPEE434 pKa = 4.43VINTVRR440 pKa = 11.84EE441 pKa = 3.96GGFVVRR447 pKa = 11.84HH448 pKa = 5.67RR449 pKa = 11.84EE450 pKa = 4.04YY451 pKa = 10.56IGDD454 pKa = 3.9ISPSQQFSSTVYY466 pKa = 10.63SINPGNANTFPWLSQVADD484 pKa = 3.45SFEE487 pKa = 4.19QYY489 pKa = 9.7EE490 pKa = 4.08FRR492 pKa = 11.84GLVFEE497 pKa = 5.1FKK499 pKa = 9.7STSSDD504 pKa = 3.17AVLSSAASSSLGTVIMATQYY524 pKa = 11.46NSLSNPFTDD533 pKa = 3.12KK534 pKa = 10.62RR535 pKa = 11.84TMEE538 pKa = 4.08NYY540 pKa = 10.06EE541 pKa = 4.04YY542 pKa = 11.24ANSDD546 pKa = 3.6KK547 pKa = 11.03PSMSFYY553 pKa = 11.07HH554 pKa = 6.54PVEE557 pKa = 4.42CKK559 pKa = 10.36KK560 pKa = 10.76SQTSIDD566 pKa = 3.61LLYY569 pKa = 11.04VRR571 pKa = 11.84NTDD574 pKa = 3.54AVNGDD579 pKa = 3.42LRR581 pKa = 11.84LYY583 pKa = 10.94DD584 pKa = 4.34LGQFQISTTGMQNTGGVIGEE604 pKa = 4.28LWCTFEE610 pKa = 4.22IAFYY614 pKa = 10.4KK615 pKa = 10.55PKK617 pKa = 10.27LLPSGTTCLTDD628 pKa = 3.69HH629 pKa = 6.53YY630 pKa = 10.23RR631 pKa = 11.84LDD633 pKa = 5.44AITTDD638 pKa = 2.78VWFGDD643 pKa = 3.98TQTLMAGSNIGTNITTGGTRR663 pKa = 11.84IQFPATLSTGKK674 pKa = 10.32FSVVYY679 pKa = 9.73SISSITGFNTPPTISGSGLNLLPLYY704 pKa = 10.86NGGTTSTVIAAQSTLSTAIIGVTVQITDD732 pKa = 3.59PNAYY736 pKa = 10.33LDD738 pKa = 4.08FVGGAIGAAAEE749 pKa = 4.22GDD751 pKa = 3.88LFISQIALEE760 pKa = 4.26ITT762 pKa = 3.52

Molecular weight: 85.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFQ5|A0A1L3KFQ5_9VIRU Uncharacterized protein OS=Changjiang tombus-like virus 21 OX=1922815 PE=4 SV=1
MM1 pKa = 7.29HH2 pKa = 7.86ASPLYY7 pKa = 10.19MSMSVSKK14 pKa = 9.66TPVYY18 pKa = 10.8GSGYY22 pKa = 9.99KK23 pKa = 10.29NGIVADD29 pKa = 4.26FFFPAGYY36 pKa = 7.42TATGDD41 pKa = 3.59TTTQQCFVSYY51 pKa = 10.2EE52 pKa = 4.15LLMQLYY58 pKa = 8.26NQNYY62 pKa = 8.54LNHH65 pKa = 5.99IFEE68 pKa = 4.64RR69 pKa = 11.84SMVYY73 pKa = 10.3KK74 pKa = 10.65KK75 pKa = 10.76LINVASNCGTMNIDD89 pKa = 2.97RR90 pKa = 11.84SYY92 pKa = 11.3DD93 pKa = 3.47LKK95 pKa = 11.32YY96 pKa = 11.15DD97 pKa = 4.07LVNNTINAAIMKK109 pKa = 9.7YY110 pKa = 10.47DD111 pKa = 3.49SLVFNYY117 pKa = 10.79AEE119 pKa = 4.61TDD121 pKa = 4.2FQSHH125 pKa = 6.84PPNTEE130 pKa = 3.57SSSVGTEE137 pKa = 3.31SRR139 pKa = 11.84MPLCQNSLTQKK150 pKa = 9.48MMSSVEE156 pKa = 4.02STARR160 pKa = 11.84NCVHH164 pKa = 5.85STRR167 pKa = 11.84ALSAFLPDD175 pKa = 4.19VILKK179 pKa = 8.19GTASLYY185 pKa = 10.53QIASGSPMYY194 pKa = 10.6LVAWRR199 pKa = 11.84NASLLKK205 pKa = 10.93LPTFLMNPYY214 pKa = 9.64WILPDD219 pKa = 3.86LLITGVVQHH228 pKa = 6.77LNHH231 pKa = 6.65YY232 pKa = 9.35YY233 pKa = 7.72PTKK236 pKa = 10.36FYY238 pKa = 10.4QLKK241 pKa = 10.19SGYY244 pKa = 9.07SNQIIAVQDD253 pKa = 3.39KK254 pKa = 10.87QNFLGAIITTKK265 pKa = 10.93NLVLNSATVILALRR279 pKa = 11.84LLLNTKK285 pKa = 9.12PIWNTNGRR293 pKa = 11.84VV294 pKa = 3.12

Molecular weight: 33.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1056

294

762

528.0

59.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.114 ± 0.718

1.136 ± 0.119

4.261 ± 0.458

3.788 ± 0.93

4.64 ± 0.479

6.629 ± 1.175

2.083 ± 0.023

6.345 ± 0.481

5.398 ± 0.339

9.564 ± 0.884

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.936 ± 0.61

7.765 ± 0.212

3.883 ± 0.287

4.545 ± 0.066

3.409 ± 0.366

7.86 ± 0.886

7.86 ± 0.02

6.439 ± 0.012

0.758 ± 0.14

5.587 ± 0.466
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski