Novosphingobium sp. GV010

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; unclassified Novosphingobium

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5048 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V5C8F3|A0A2V5C8F3_9SPHN DNA-binding transcriptional LysR family regulator OS=Novosphingobium sp. GV010 OX=2135477 GN=C7493_108153 PE=3 SV=1
MM1 pKa = 7.17ATIFRR6 pKa = 11.84SGYY9 pKa = 10.55GDD11 pKa = 3.48WSDD14 pKa = 3.24MADD17 pKa = 3.24WYY19 pKa = 11.15GHH21 pKa = 6.33AVPTAADD28 pKa = 3.41DD29 pKa = 5.14AVFQGNALYY38 pKa = 10.73AGITGVAQAAGLSLADD54 pKa = 3.65GSGIGVGQGGTLTVGDD70 pKa = 4.35GGIALASSAWLQIVGTGTTATTTGTLTLDD99 pKa = 4.03DD100 pKa = 4.46ASVAVMLGEE109 pKa = 4.53EE110 pKa = 5.0GSASDD115 pKa = 3.73VTLSASALDD124 pKa = 3.7SRR126 pKa = 11.84GTIQLHH132 pKa = 5.77AAGGSHH138 pKa = 6.9ARR140 pKa = 11.84LLVSGAAGFGTAGVLTGSVLLDD162 pKa = 3.67GASTIAFGSGQITAIAGDD180 pKa = 3.97LTLSGAQATITSGEE194 pKa = 4.14VAGNSALTGLTSLAGTLTLTGLALTLTGDD223 pKa = 3.96LDD225 pKa = 3.84VDD227 pKa = 3.99GALQLGSSDD236 pKa = 3.51PVASMRR242 pKa = 11.84ATTDD246 pKa = 3.05LTVAGTLTGNGIVQILGTAANGAATLTVGGLAHH279 pKa = 6.67TGFVAITGTANAAAMLVDD297 pKa = 4.23TGAAPATLTGSLLLTDD313 pKa = 4.23HH314 pKa = 7.08ASVHH318 pKa = 5.81LASGTIAQIAQGSTVTLSDD337 pKa = 2.94ATSFVSSEE345 pKa = 4.16ADD347 pKa = 3.18TASNSGLAGLSVVAGALTLNGGGLAITGDD376 pKa = 4.11LGNSGTIRR384 pKa = 11.84LNNGSDD390 pKa = 3.87PDD392 pKa = 3.9TGAATALAATIAGTLTNSGILSLMADD418 pKa = 4.01GSYY421 pKa = 11.41GSGSVGLAVAGIDD434 pKa = 3.5NTPDD438 pKa = 3.35GNIMIAGNADD448 pKa = 3.48TGDD451 pKa = 3.64TAILAVASAAPAALDD466 pKa = 3.74GLWQVGANGSVVFASGGIADD486 pKa = 4.5IVASGQLVLSSASAVFANAADD507 pKa = 3.96TASNSALSSLASIEE521 pKa = 4.03GVLAIASGSVVLGGALNNSGGIYY544 pKa = 10.3LATGSSGPGTAPDD557 pKa = 3.73TASLSIAGVLTNSGTITIGGDD578 pKa = 2.93NSAATLTTGGLINTGSLSVTANVAMAINGLLVNDD612 pKa = 4.31GVMDD616 pKa = 3.96LTLAQRR622 pKa = 11.84LDD624 pKa = 3.78LTGVTALTLGAQASTMLGLAQDD646 pKa = 4.39GPLVSDD652 pKa = 4.06TLAIDD657 pKa = 4.3GGTLTVDD664 pKa = 3.13AGGVPLNAGDD674 pKa = 3.98RR675 pKa = 11.84FTIFSFGAGSANGLFDD691 pKa = 3.82TFSLSSIGAGIGGYY705 pKa = 7.38VQHH708 pKa = 7.19GDD710 pKa = 3.68LLIGLVYY717 pKa = 9.92EE718 pKa = 4.32DD719 pKa = 3.2HH720 pKa = 7.14AGRR723 pKa = 11.84IEE725 pKa = 4.28MVVAEE730 pKa = 5.16APASTADD737 pKa = 3.36SFATSGGAWEE747 pKa = 6.18DD748 pKa = 3.27IAKK751 pKa = 7.08WTQGAPAFYY760 pKa = 9.47AQASIAHH767 pKa = 6.42AAVTLSSDD775 pKa = 3.33ATVSTLALTDD785 pKa = 3.69GATLGVGVLSDD796 pKa = 4.03LSVTGALTVGEE807 pKa = 4.58GTRR810 pKa = 11.84LTVTGGALASAGTQQVDD827 pKa = 2.94GHH829 pKa = 6.89LILTGGAQAFLHH841 pKa = 5.45GTLYY845 pKa = 11.04GGGTIALAGSAVLHH859 pKa = 5.89VEE861 pKa = 4.18GSVAAGLTIDD871 pKa = 4.57FGAAGVGDD879 pKa = 4.41EE880 pKa = 5.28MILTTPGAFDD890 pKa = 3.27GTIAGFAPGDD900 pKa = 3.8TLDD903 pKa = 3.76LAGIAGAHH911 pKa = 5.85LVLGAGTQGGTQLLVEE927 pKa = 5.24DD928 pKa = 4.39GAGTVLVTLAFEE940 pKa = 4.47GAYY943 pKa = 10.04GLSSFTLTQDD953 pKa = 3.21GAGGSLLSLAGNQPSPLTIGAVSNTVVEE981 pKa = 4.63SGAGVAGIATGTALATATDD1000 pKa = 3.6ASFVLTGFTHH1010 pKa = 6.88IGGTVWSEE1018 pKa = 3.38QGTYY1022 pKa = 8.19GTLMLDD1028 pKa = 3.64TATGGLTYY1036 pKa = 10.5TLDD1039 pKa = 3.64NASAATNALSGGAITADD1056 pKa = 3.2RR1057 pKa = 11.84FTIAVTDD1064 pKa = 3.52THH1066 pKa = 6.86GLTTSQAVSFAITGTNDD1083 pKa = 2.94APVVADD1089 pKa = 3.29KK1090 pKa = 9.72TQAVAYY1096 pKa = 9.05NGSAAMTAAALLVGASDD1113 pKa = 4.31AEE1115 pKa = 4.29GDD1117 pKa = 3.67ALSVVGASGAQHH1129 pKa = 6.08GTLSMDD1135 pKa = 3.7ADD1137 pKa = 3.99GTVHH1141 pKa = 6.48YY1142 pKa = 10.33SAFQAYY1148 pKa = 9.19VGADD1152 pKa = 3.16SYY1154 pKa = 11.51QVVVSDD1160 pKa = 3.35SHH1162 pKa = 6.76GATQVEE1168 pKa = 4.87TVKK1171 pKa = 9.58VTVSGATTAAATTTPAYY1188 pKa = 9.55VWAGASTTGQVFDD1201 pKa = 4.02VSGDD1205 pKa = 3.7GLGHH1209 pKa = 7.05RR1210 pKa = 11.84LYY1212 pKa = 10.59ATGGDD1217 pKa = 3.92DD1218 pKa = 4.58TIHH1221 pKa = 6.64GGNGGDD1227 pKa = 4.1ALNGGAGNDD1236 pKa = 3.65IIYY1239 pKa = 10.77GGDD1242 pKa = 3.57GKK1244 pKa = 9.95DD1245 pKa = 3.36TLTGGAGADD1254 pKa = 3.91RR1255 pKa = 11.84VWGGAGADD1263 pKa = 3.18TFVFRR1268 pKa = 11.84PGDD1271 pKa = 3.59MADD1274 pKa = 3.48PALTGGLYY1282 pKa = 10.63DD1283 pKa = 5.02EE1284 pKa = 5.57IMDD1287 pKa = 4.58FSGAGNGYY1295 pKa = 10.06RR1296 pKa = 11.84PGEE1299 pKa = 4.21DD1300 pKa = 2.6MLVFSGFSSAATLTYY1315 pKa = 10.79DD1316 pKa = 4.21VAATQAASDD1325 pKa = 3.96ATAHH1329 pKa = 6.36YY1330 pKa = 8.35YY1331 pKa = 9.66TVTDD1335 pKa = 4.49GAWHH1339 pKa = 6.75GDD1341 pKa = 3.5IIVHH1345 pKa = 4.44YY1346 pKa = 8.81TGNAVLQLGDD1356 pKa = 3.66YY1357 pKa = 11.07GFLL1360 pKa = 3.71

Molecular weight: 131.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V5B5Y2|A0A2V5B5Y2_9SPHN Uncharacterized protein OS=Novosphingobium sp. GV010 OX=2135477 GN=C7493_107132 PE=4 SV=1
MM1 pKa = 8.47AEE3 pKa = 3.94MARR6 pKa = 11.84NGMTSLPAIGFMLATVAMVVLATVMWFVALVTLLAVVGLRR46 pKa = 11.84RR47 pKa = 11.84PLRR50 pKa = 11.84SRR52 pKa = 11.84QGRR55 pKa = 11.84MIRR58 pKa = 11.84MARR61 pKa = 11.84RR62 pKa = 11.84AGSARR67 pKa = 11.84PP68 pKa = 3.43

Molecular weight: 7.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5048

0

5048

1713247

26

6640

339.4

36.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.38 ± 0.052

0.802 ± 0.01

5.78 ± 0.028

4.443 ± 0.034

3.446 ± 0.021

9.266 ± 0.099

2.293 ± 0.018

4.788 ± 0.022

2.624 ± 0.027

9.98 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.581 ± 0.018

2.614 ± 0.028

5.48 ± 0.038

3.411 ± 0.021

6.928 ± 0.038

5.338 ± 0.038

5.761 ± 0.038

7.301 ± 0.029

1.482 ± 0.018

2.303 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski