Amino acid dipepetide frequency for Novosphingobium sp. GV010

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.121AlaAla: 18.121 ± 0.143
1.178AlaCys: 1.178 ± 0.026
7.401AlaAsp: 7.401 ± 0.07
5.755AlaGlu: 5.755 ± 0.071
4.25AlaPhe: 4.25 ± 0.054
12.017AlaGly: 12.017 ± 0.139
2.928AlaHis: 2.928 ± 0.052
6.436AlaIle: 6.436 ± 0.068
3.35AlaLys: 3.35 ± 0.057
14.69AlaLeu: 14.69 ± 0.129
4.371AlaMet: 4.371 ± 0.059
3.206AlaAsn: 3.206 ± 0.056
7.045AlaPro: 7.045 ± 0.08
5.749AlaGln: 5.749 ± 0.079
10.014AlaArg: 10.014 ± 0.105
6.886AlaSer: 6.886 ± 0.07
7.204AlaThr: 7.204 ± 0.086
8.685AlaVal: 8.685 ± 0.092
1.763AlaTrp: 1.763 ± 0.036
2.749AlaTyr: 2.749 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
1.064CysAla: 1.064 ± 0.025
0.103CysCys: 0.103 ± 0.008
0.507CysAsp: 0.507 ± 0.017
0.336CysGlu: 0.336 ± 0.017
0.292CysPhe: 0.292 ± 0.013
0.828CysGly: 0.828 ± 0.021
0.244CysHis: 0.244 ± 0.012
0.326CysIle: 0.326 ± 0.014
0.13CysLys: 0.13 ± 0.009
0.758CysLeu: 0.758 ± 0.02
0.191CysMet: 0.191 ± 0.011
0.2CysAsn: 0.2 ± 0.01
0.444CysPro: 0.444 ± 0.016
0.216CysGln: 0.216 ± 0.009
0.559CysArg: 0.559 ± 0.02
0.448CysSer: 0.448 ± 0.017
0.422CysThr: 0.422 ± 0.017
0.628CysVal: 0.628 ± 0.021
0.128CysTrp: 0.128 ± 0.01
0.197CysTyr: 0.197 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
7.225AspAla: 7.225 ± 0.073
0.428AspCys: 0.428 ± 0.016
2.98AspAsp: 2.98 ± 0.05
2.7AspGlu: 2.7 ± 0.045
2.094AspPhe: 2.094 ± 0.036
5.448AspGly: 5.448 ± 0.073
1.623AspHis: 1.623 ± 0.037
2.917AspIle: 2.917 ± 0.044
1.614AspLys: 1.614 ± 0.038
6.137AspLeu: 6.137 ± 0.062
1.448AspMet: 1.448 ± 0.029
1.384AspAsn: 1.384 ± 0.032
3.595AspPro: 3.595 ± 0.047
1.85AspGln: 1.85 ± 0.037
4.318AspArg: 4.318 ± 0.055
2.174AspSer: 2.174 ± 0.044
3.059AspThr: 3.059 ± 0.048
4.037AspVal: 4.037 ± 0.048
1.131AspTrp: 1.131 ± 0.028
1.638AspTyr: 1.638 ± 0.035
0.0AspXaa: 0.0 ± 0.0
Glu
6.555GluAla: 6.555 ± 0.085
0.278GluCys: 0.278 ± 0.014
2.334GluAsp: 2.334 ± 0.041
2.361GluGlu: 2.361 ± 0.044
1.241GluPhe: 1.241 ± 0.032
3.928GluGly: 3.928 ± 0.05
1.062GluHis: 1.062 ± 0.025
2.355GluIle: 2.355 ± 0.04
1.476GluLys: 1.476 ± 0.034
3.806GluLeu: 3.806 ± 0.06
1.199GluMet: 1.199 ± 0.026
1.104GluAsn: 1.104 ± 0.025
2.111GluPro: 2.111 ± 0.04
1.813GluGln: 1.813 ± 0.038
3.837GluArg: 3.837 ± 0.054
1.846GluSer: 1.846 ± 0.036
2.683GluThr: 2.683 ± 0.041
3.074GluVal: 3.074 ± 0.043
0.626GluTrp: 0.626 ± 0.021
0.747GluTyr: 0.747 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
4.816PheAla: 4.816 ± 0.069
0.337PheCys: 0.337 ± 0.013
2.517PheAsp: 2.517 ± 0.041
1.645PheGlu: 1.645 ± 0.032
1.194PhePhe: 1.194 ± 0.032
3.503PheGly: 3.503 ± 0.05
0.811PheHis: 0.811 ± 0.023
1.411PheIle: 1.411 ± 0.027
0.787PheLys: 0.787 ± 0.022
2.926PheLeu: 2.926 ± 0.053
0.796PheMet: 0.796 ± 0.024
1.158PheAsn: 1.158 ± 0.028
1.542PhePro: 1.542 ± 0.029
0.894PheGln: 0.894 ± 0.026
1.997PheArg: 1.997 ± 0.034
2.092PheSer: 2.092 ± 0.037
2.118PheThr: 2.118 ± 0.038
2.503PheVal: 2.503 ± 0.043
0.484PheTrp: 0.484 ± 0.018
0.927PheTyr: 0.927 ± 0.023
0.0PheXaa: 0.0 ± 0.0
Gly
10.856GlyAla: 10.856 ± 0.172
0.85GlyCys: 0.85 ± 0.024
4.96GlyAsp: 4.96 ± 0.076
4.177GlyGlu: 4.177 ± 0.048
3.802GlyPhe: 3.802 ± 0.06
8.994GlyGly: 8.994 ± 0.508
2.289GlyHis: 2.289 ± 0.038
4.346GlyIle: 4.346 ± 0.048
3.132GlyLys: 3.132 ± 0.045
9.296GlyLeu: 9.296 ± 0.068
2.569GlyMet: 2.569 ± 0.04
2.36GlyAsn: 2.36 ± 0.073
3.954GlyPro: 3.954 ± 0.05
3.501GlyGln: 3.501 ± 0.048
6.01GlyArg: 6.01 ± 0.071
4.972GlySer: 4.972 ± 0.118
5.584GlyThr: 5.584 ± 0.119
6.827GlyVal: 6.827 ± 0.076
1.778GlyTrp: 1.778 ± 0.035
2.404GlyTyr: 2.404 ± 0.046
0.0GlyXaa: 0.0 ± 0.0
His
2.952HisAla: 2.952 ± 0.042
0.224HisCys: 0.224 ± 0.011
1.474HisAsp: 1.474 ± 0.034
0.998HisGlu: 0.998 ± 0.028
0.918HisPhe: 0.918 ± 0.02
2.346HisGly: 2.346 ± 0.043
0.671HisHis: 0.671 ± 0.022
1.033HisIle: 1.033 ± 0.027
0.461HisLys: 0.461 ± 0.015
2.228HisLeu: 2.228 ± 0.037
0.504HisMet: 0.504 ± 0.018
0.519HisAsn: 0.519 ± 0.018
1.498HisPro: 1.498 ± 0.029
0.662HisGln: 0.662 ± 0.019
1.642HisArg: 1.642 ± 0.034
0.985HisSer: 0.985 ± 0.028
1.007HisThr: 1.007 ± 0.022
1.718HisVal: 1.718 ± 0.034
0.426HisTrp: 0.426 ± 0.015
0.669HisTyr: 0.669 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
7.584IleAla: 7.584 ± 0.066
0.43IleCys: 0.43 ± 0.016
3.653IleAsp: 3.653 ± 0.053
2.898IleGlu: 2.898 ± 0.044
1.447IlePhe: 1.447 ± 0.033
4.63IleGly: 4.63 ± 0.061
0.963IleHis: 0.963 ± 0.024
1.858IleIle: 1.858 ± 0.038
1.15IleLys: 1.15 ± 0.029
3.713IleLeu: 3.713 ± 0.058
0.94IleMet: 0.94 ± 0.024
1.373IleAsn: 1.373 ± 0.028
2.244IlePro: 2.244 ± 0.038
1.148IleGln: 1.148 ± 0.025
2.928IleArg: 2.928 ± 0.046
2.475IleSer: 2.475 ± 0.037
2.956IleThr: 2.956 ± 0.045
3.871IleVal: 3.871 ± 0.051
0.611IleTrp: 0.611 ± 0.022
1.012IleTyr: 1.012 ± 0.023
0.0IleXaa: 0.0 ± 0.0
Lys
3.743LysAla: 3.743 ± 0.055
0.107LysCys: 0.107 ± 0.008
1.435LysAsp: 1.435 ± 0.034
0.996LysGlu: 0.996 ± 0.026
0.76LysPhe: 0.76 ± 0.021
2.617LysGly: 2.617 ± 0.042
0.481LysHis: 0.481 ± 0.017
1.26LysIle: 1.26 ± 0.032
0.784LysLys: 0.784 ± 0.026
2.581LysLeu: 2.581 ± 0.048
0.615LysMet: 0.615 ± 0.02
0.66LysAsn: 0.66 ± 0.02
1.82LysPro: 1.82 ± 0.043
0.8LysGln: 0.8 ± 0.024
1.776LysArg: 1.776 ± 0.033
1.352LysSer: 1.352 ± 0.033
1.586LysThr: 1.586 ± 0.034
2.09LysVal: 2.09 ± 0.036
0.302LysTrp: 0.302 ± 0.012
0.473LysTyr: 0.473 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
14.846LeuAla: 14.846 ± 0.128
0.894LeuCys: 0.894 ± 0.023
5.937LeuAsp: 5.937 ± 0.067
4.025LeuGlu: 4.025 ± 0.062
3.3LeuPhe: 3.3 ± 0.05
8.953LeuGly: 8.953 ± 0.083
2.084LeuHis: 2.084 ± 0.036
4.706LeuIle: 4.706 ± 0.054
2.674LeuLys: 2.674 ± 0.044
9.086LeuLeu: 9.086 ± 0.112
2.401LeuMet: 2.401 ± 0.041
2.523LeuAsn: 2.523 ± 0.054
5.921LeuPro: 5.921 ± 0.061
2.422LeuGln: 2.422 ± 0.037
6.937LeuArg: 6.937 ± 0.072
6.423LeuSer: 6.423 ± 0.069
6.291LeuThr: 6.291 ± 0.085
7.014LeuVal: 7.014 ± 0.078
1.267LeuTrp: 1.267 ± 0.029
2.096LeuTyr: 2.096 ± 0.038
0.0LeuXaa: 0.0 ± 0.0
Met
3.796MetAla: 3.796 ± 0.051
0.138MetCys: 0.138 ± 0.01
1.151MetAsp: 1.151 ± 0.027
0.979MetGlu: 0.979 ± 0.027
0.678MetPhe: 0.678 ± 0.023
2.168MetGly: 2.168 ± 0.038
0.468MetHis: 0.468 ± 0.016
1.326MetIle: 1.326 ± 0.028
0.825MetLys: 0.825 ± 0.021
2.725MetLeu: 2.725 ± 0.051
0.754MetMet: 0.754 ± 0.024
0.674MetAsn: 0.674 ± 0.02
1.625MetPro: 1.625 ± 0.03
0.819MetGln: 0.819 ± 0.021
1.842MetArg: 1.842 ± 0.036
1.497MetSer: 1.497 ± 0.031
1.897MetThr: 1.897 ± 0.035
1.947MetVal: 1.947 ± 0.037
0.221MetTrp: 0.221 ± 0.011
0.281MetTyr: 0.281 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
3.467AsnAla: 3.467 ± 0.055
0.205AsnCys: 0.205 ± 0.012
1.365AsnAsp: 1.365 ± 0.031
0.932AsnGlu: 0.932 ± 0.028
0.927AsnPhe: 0.927 ± 0.029
2.668AsnGly: 2.668 ± 0.079
0.552AsnHis: 0.552 ± 0.018
1.237AsnIle: 1.237 ± 0.027
0.595AsnLys: 0.595 ± 0.02
2.62AsnLeu: 2.62 ± 0.049
0.491AsnMet: 0.491 ± 0.018
0.78AsnAsn: 0.78 ± 0.028
1.793AsnPro: 1.793 ± 0.038
0.811AsnGln: 0.811 ± 0.022
1.768AsnArg: 1.768 ± 0.035
1.316AsnSer: 1.316 ± 0.033
1.422AsnThr: 1.422 ± 0.034
1.965AsnVal: 1.965 ± 0.04
0.437AsnTrp: 0.437 ± 0.018
0.784AsnTyr: 0.784 ± 0.031
0.0AsnXaa: 0.0 ± 0.0
Pro
7.671ProAla: 7.671 ± 0.078
0.379ProCys: 0.379 ± 0.014
3.927ProAsp: 3.927 ± 0.052
2.844ProGlu: 2.844 ± 0.046
1.958ProPhe: 1.958 ± 0.033
5.239ProGly: 5.239 ± 0.057
1.224ProHis: 1.224 ± 0.027
2.264ProIle: 2.264 ± 0.041
1.236ProLys: 1.236 ± 0.027
5.222ProLeu: 5.222 ± 0.067
1.414ProMet: 1.414 ± 0.03
1.192ProAsn: 1.192 ± 0.031
2.774ProPro: 2.774 ± 0.055
1.95ProGln: 1.95 ± 0.031
3.264ProArg: 3.264 ± 0.049
2.756ProSer: 2.756 ± 0.05
2.785ProThr: 2.785 ± 0.046
4.711ProVal: 4.711 ± 0.066
0.777ProTrp: 0.777 ± 0.024
1.209ProTyr: 1.209 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
4.881GlnAla: 4.881 ± 0.068
0.204GlnCys: 0.204 ± 0.012
1.758GlnAsp: 1.758 ± 0.035
1.243GlnGlu: 1.243 ± 0.026
1.071GlnPhe: 1.071 ± 0.029
3.162GlnGly: 3.162 ± 0.05
0.73GlnHis: 0.73 ± 0.021
1.88GlnIle: 1.88 ± 0.035
0.831GlnLys: 0.831 ± 0.024
2.909GlnLeu: 2.909 ± 0.05
0.918GlnMet: 0.918 ± 0.024
0.881GlnAsn: 0.881 ± 0.025
2.012GlnPro: 2.012 ± 0.034
1.342GlnGln: 1.342 ± 0.033
2.409GlnArg: 2.409 ± 0.037
1.99GlnSer: 1.99 ± 0.061
2.052GlnThr: 2.052 ± 0.04
2.649GlnVal: 2.649 ± 0.042
0.521GlnTrp: 0.521 ± 0.017
0.671GlnTyr: 0.671 ± 0.02
0.0GlnXaa: 0.0 ± 0.0
Arg
8.336ArgAla: 8.336 ± 0.087
0.493ArgCys: 0.493 ± 0.018
4.175ArgAsp: 4.175 ± 0.051
3.193ArgGlu: 3.193 ± 0.049
2.794ArgPhe: 2.794 ± 0.044
5.013ArgGly: 5.013 ± 0.06
1.944ArgHis: 1.944 ± 0.039
3.803ArgIle: 3.803 ± 0.05
1.863ArgLys: 1.863 ± 0.033
8.027ArgLeu: 8.027 ± 0.083
1.947ArgMet: 1.947 ± 0.03
1.811ArgAsn: 1.811 ± 0.031
3.624ArgPro: 3.624 ± 0.058
2.671ArgGln: 2.671 ± 0.038
5.319ArgArg: 5.319 ± 0.071
3.415ArgSer: 3.415 ± 0.051
3.12ArgThr: 3.12 ± 0.043
4.698ArgVal: 4.698 ± 0.054
1.219ArgTrp: 1.219 ± 0.029
1.815ArgTyr: 1.815 ± 0.037
0.0ArgXaa: 0.0 ± 0.0
Ser
6.716SerAla: 6.716 ± 0.074
0.413SerCys: 0.413 ± 0.015
2.913SerAsp: 2.913 ± 0.043
2.049SerGlu: 2.049 ± 0.037
2.193SerPhe: 2.193 ± 0.037
5.687SerGly: 5.687 ± 0.111
1.176SerHis: 1.176 ± 0.029
2.667SerIle: 2.667 ± 0.055
1.171SerLys: 1.171 ± 0.031
5.352SerLeu: 5.352 ± 0.071
1.249SerMet: 1.249 ± 0.024
1.404SerAsn: 1.404 ± 0.034
3.016SerPro: 3.016 ± 0.042
1.69SerGln: 1.69 ± 0.032
3.326SerArg: 3.326 ± 0.044
3.075SerSer: 3.075 ± 0.071
3.035SerThr: 3.035 ± 0.067
3.98SerVal: 3.98 ± 0.084
0.784SerTrp: 0.784 ± 0.023
1.481SerTyr: 1.481 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
6.858ThrAla: 6.858 ± 0.075
0.427ThrCys: 0.427 ± 0.016
2.825ThrAsp: 2.825 ± 0.04
1.835ThrGlu: 1.835 ± 0.037
1.929ThrPhe: 1.929 ± 0.036
6.091ThrGly: 6.091 ± 0.114
1.237ThrHis: 1.237 ± 0.03
3.003ThrIle: 3.003 ± 0.044
1.195ThrLys: 1.195 ± 0.031
6.549ThrLeu: 6.549 ± 0.08
1.345ThrMet: 1.345 ± 0.03
1.535ThrAsn: 1.535 ± 0.043
3.896ThrPro: 3.896 ± 0.054
2.016ThrGln: 2.016 ± 0.034
3.57ThrArg: 3.57 ± 0.047
3.206ThrSer: 3.206 ± 0.075
3.361ThrThr: 3.361 ± 0.07
4.501ThrVal: 4.501 ± 0.077
0.723ThrTrp: 0.723 ± 0.019
1.506ThrTyr: 1.506 ± 0.037
0.0ThrXaa: 0.0 ± 0.0
Val
9.951ValAla: 9.951 ± 0.086
0.601ValCys: 0.601 ± 0.02
4.17ValAsp: 4.17 ± 0.047
3.952ValGlu: 3.952 ± 0.054
2.202ValPhe: 2.202 ± 0.037
6.001ValGly: 6.001 ± 0.103
1.432ValHis: 1.432 ± 0.032
3.576ValIle: 3.576 ± 0.051
1.978ValLys: 1.978 ± 0.042
7.273ValLeu: 7.273 ± 0.079
1.863ValMet: 1.863 ± 0.035
2.153ValAsn: 2.153 ± 0.041
3.974ValPro: 3.974 ± 0.053
2.218ValGln: 2.218 ± 0.039
4.537ValArg: 4.537 ± 0.053
4.351ValSer: 4.351 ± 0.061
4.771ValThr: 4.771 ± 0.079
5.636ValVal: 5.636 ± 0.061
0.992ValTrp: 0.992 ± 0.029
1.378ValTyr: 1.378 ± 0.031
0.0ValXaa: 0.0 ± 0.0
Trp
1.532TrpAla: 1.532 ± 0.029
0.137TrpCys: 0.137 ± 0.01
0.695TrpAsp: 0.695 ± 0.023
0.511TrpGlu: 0.511 ± 0.017
0.557TrpPhe: 0.557 ± 0.018
1.056TrpGly: 1.056 ± 0.025
0.44TrpHis: 0.44 ± 0.015
0.64TrpIle: 0.64 ± 0.022
0.416TrpLys: 0.416 ± 0.016
1.815TrpLeu: 1.815 ± 0.043
0.375TrpMet: 0.375 ± 0.015
0.472TrpAsn: 0.472 ± 0.018
0.796TrpPro: 0.796 ± 0.023
0.731TrpGln: 0.731 ± 0.022
1.367TrpArg: 1.367 ± 0.031
0.926TrpSer: 0.926 ± 0.022
0.871TrpThr: 0.871 ± 0.026
0.898TrpVal: 0.898 ± 0.023
0.264TrpTrp: 0.264 ± 0.012
0.317TrpTyr: 0.317 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.824TyrAla: 2.824 ± 0.042
0.193TyrCys: 0.193 ± 0.01
1.625TyrAsp: 1.625 ± 0.031
1.005TyrGlu: 1.005 ± 0.028
0.855TyrPhe: 0.855 ± 0.025
2.314TyrGly: 2.314 ± 0.041
0.576TyrHis: 0.576 ± 0.018
0.838TyrIle: 0.838 ± 0.024
0.57TyrLys: 0.57 ± 0.022
2.086TyrLeu: 2.086 ± 0.034
0.421TyrMet: 0.421 ± 0.016
0.748TyrAsn: 0.748 ± 0.027
1.114TyrPro: 1.114 ± 0.028
0.808TyrGln: 0.808 ± 0.025
1.858TyrArg: 1.858 ± 0.034
1.195TyrSer: 1.195 ± 0.03
1.383TyrThr: 1.383 ± 0.033
1.579TyrVal: 1.579 ± 0.033
0.362TyrTrp: 0.362 ± 0.014
0.672TyrTyr: 0.672 ± 0.023
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5048 proteins (1713247 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski