Microbacterium phage TimoTea

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481VUJ3|A0A481VUJ3_9CAUD Tape measure protein OS=Microbacterium phage TimoTea OX=2530119 GN=12 PE=4 SV=1
MM1 pKa = 6.87EE2 pKa = 4.73TSTKK6 pKa = 10.52KK7 pKa = 10.49FDD9 pKa = 4.67LDD11 pKa = 3.48QLTLGEE17 pKa = 4.17VAAIEE22 pKa = 4.46DD23 pKa = 3.97LSGVAIGSVSEE34 pKa = 4.27STPQGKK40 pKa = 8.64FLAALYY46 pKa = 8.8MVAKK50 pKa = 10.31RR51 pKa = 11.84RR52 pKa = 11.84DD53 pKa = 3.68GQPTFTFNAALQASMAEE70 pKa = 4.08AQSFLGFDD78 pKa = 4.01APDD81 pKa = 3.73AADD84 pKa = 4.36DD85 pKa = 4.04ADD87 pKa = 3.92AAEE90 pKa = 5.11SSAEE94 pKa = 4.06GNGDD98 pKa = 3.59SSPEE102 pKa = 3.75NAPAA106 pKa = 4.34

Molecular weight: 10.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481VTV4|A0A481VTV4_9CAUD Uncharacterized protein OS=Microbacterium phage TimoTea OX=2530119 GN=16 PE=4 SV=1
MM1 pKa = 7.94AEE3 pKa = 3.9YY4 pKa = 10.13QNRR7 pKa = 11.84GIRR10 pKa = 11.84IDD12 pKa = 3.73GLRR15 pKa = 11.84EE16 pKa = 3.64LNAKK20 pKa = 9.43LRR22 pKa = 11.84AAGDD26 pKa = 3.72EE27 pKa = 4.39SADD30 pKa = 4.17LPDD33 pKa = 3.73LMFRR37 pKa = 11.84LGSIVIANARR47 pKa = 11.84VPAKK51 pKa = 10.34SGEE54 pKa = 4.09LAGTLRR60 pKa = 11.84AGRR63 pKa = 11.84GRR65 pKa = 11.84TKK67 pKa = 10.34AVVRR71 pKa = 11.84AGYY74 pKa = 10.43AKK76 pKa = 10.18RR77 pKa = 11.84GAHH80 pKa = 6.33AGVVHH85 pKa = 6.46YY86 pKa = 9.46GNPHH90 pKa = 5.42TGSRR94 pKa = 11.84AQPFLVDD101 pKa = 3.69ALRR104 pKa = 11.84RR105 pKa = 11.84AQSQLVSEE113 pKa = 4.67LQTGIDD119 pKa = 3.26QLMRR123 pKa = 11.84KK124 pKa = 9.03HH125 pKa = 6.36KK126 pKa = 10.6LL127 pKa = 3.21

Molecular weight: 13.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25

0

25

5502

70

701

220.1

23.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.959 ± 0.555

0.345 ± 0.115

5.507 ± 0.553

5.398 ± 0.523

3.017 ± 0.284

8.997 ± 0.599

1.509 ± 0.27

4.489 ± 0.353

1.981 ± 0.278

9.542 ± 0.837

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.999 ± 0.236

2.963 ± 0.308

5.107 ± 0.313

4.162 ± 0.318

7.397 ± 0.7

5.852 ± 0.391

6.598 ± 0.522

7.561 ± 0.496

1.418 ± 0.171

2.199 ± 0.26
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski