Amino acid dipepetide frequency for Microbacterium phage TimoTea

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.178AlaAla: 22.178 ± 2.337
0.182AlaCys: 0.182 ± 0.139
8.726AlaAsp: 8.726 ± 1.436
8.362AlaGlu: 8.362 ± 1.215
5.09AlaPhe: 5.09 ± 0.742
16.906AlaGly: 16.906 ± 1.793
1.454AlaHis: 1.454 ± 0.692
5.272AlaIle: 5.272 ± 1.041
4.545AlaLys: 4.545 ± 0.85
13.452AlaLeu: 13.452 ± 1.511
3.272AlaMet: 3.272 ± 0.913
2.545AlaAsn: 2.545 ± 0.824
8.726AlaPro: 8.726 ± 1.186
4.726AlaGln: 4.726 ± 1.003
8.362AlaArg: 8.362 ± 1.256
5.454AlaSer: 5.454 ± 1.014
7.453AlaThr: 7.453 ± 1.548
7.999AlaVal: 7.999 ± 1.683
2.0AlaTrp: 2.0 ± 0.49
2.727AlaTyr: 2.727 ± 0.834
0.0AlaXaa: 0.0 ± 0.0
Cys
0.182CysAla: 0.182 ± 0.235
0.0CysCys: 0.0 ± 0.0
0.182CysAsp: 0.182 ± 0.18
0.364CysGlu: 0.364 ± 0.217
0.182CysPhe: 0.182 ± 0.18
0.182CysGly: 0.182 ± 0.176
0.0CysHis: 0.0 ± 0.0
0.0CysIle: 0.0 ± 0.0
0.182CysLys: 0.182 ± 0.186
0.364CysLeu: 0.364 ± 0.248
0.0CysMet: 0.0 ± 0.0
0.364CysAsn: 0.364 ± 0.225
0.0CysPro: 0.0 ± 0.0
0.182CysGln: 0.182 ± 0.176
0.364CysArg: 0.364 ± 0.244
0.0CysSer: 0.0 ± 0.0
0.545CysThr: 0.545 ± 0.374
0.0CysVal: 0.0 ± 0.0
0.0CysTrp: 0.0 ± 0.0
0.364CysTyr: 0.364 ± 0.21
0.0CysXaa: 0.0 ± 0.0
Asp
10.18AspAla: 10.18 ± 1.8
0.182AspCys: 0.182 ± 0.175
3.272AspAsp: 3.272 ± 1.091
3.636AspGlu: 3.636 ± 1.429
0.727AspPhe: 0.727 ± 0.498
5.454AspGly: 5.454 ± 1.051
0.727AspHis: 0.727 ± 0.386
0.545AspIle: 0.545 ± 0.256
0.182AspLys: 0.182 ± 0.193
7.999AspLeu: 7.999 ± 1.496
0.727AspMet: 0.727 ± 0.465
1.091AspAsn: 1.091 ± 0.421
2.727AspPro: 2.727 ± 0.624
1.454AspGln: 1.454 ± 0.677
3.09AspArg: 3.09 ± 0.765
2.363AspSer: 2.363 ± 0.609
3.272AspThr: 3.272 ± 0.954
5.272AspVal: 5.272 ± 1.056
0.727AspTrp: 0.727 ± 0.397
1.454AspTyr: 1.454 ± 0.42
0.0AspXaa: 0.0 ± 0.0
Glu
6.544GluAla: 6.544 ± 1.027
0.182GluCys: 0.182 ± 0.176
3.09GluAsp: 3.09 ± 0.646
0.727GluGlu: 0.727 ± 0.412
0.727GluPhe: 0.727 ± 0.33
3.999GluGly: 3.999 ± 0.925
0.545GluHis: 0.545 ± 0.282
3.999GluIle: 3.999 ± 0.684
0.364GluLys: 0.364 ± 0.239
8.544GluLeu: 8.544 ± 1.338
0.364GluMet: 0.364 ± 0.215
3.09GluAsn: 3.09 ± 0.996
1.636GluPro: 1.636 ± 0.621
3.454GluGln: 3.454 ± 0.871
4.908GluArg: 4.908 ± 1.536
3.454GluSer: 3.454 ± 0.761
3.454GluThr: 3.454 ± 0.753
2.181GluVal: 2.181 ± 0.419
1.272GluTrp: 1.272 ± 0.617
1.454GluTyr: 1.454 ± 0.452
0.0GluXaa: 0.0 ± 0.0
Phe
4.363PheAla: 4.363 ± 0.949
0.0PheCys: 0.0 ± 0.0
2.363PheAsp: 2.363 ± 0.588
1.454PheGlu: 1.454 ± 0.611
0.182PhePhe: 0.182 ± 0.139
3.817PheGly: 3.817 ± 0.996
0.182PheHis: 0.182 ± 0.235
1.091PheIle: 1.091 ± 0.279
0.545PheLys: 0.545 ± 0.24
2.727PheLeu: 2.727 ± 0.648
0.909PheMet: 0.909 ± 0.276
1.454PheAsn: 1.454 ± 0.454
0.727PhePro: 0.727 ± 0.289
1.091PheGln: 1.091 ± 0.589
1.454PheArg: 1.454 ± 0.534
0.909PheSer: 0.909 ± 0.45
3.272PheThr: 3.272 ± 0.898
2.727PheVal: 2.727 ± 0.588
0.182PheTrp: 0.182 ± 0.169
0.727PheTyr: 0.727 ± 0.453
0.0PheXaa: 0.0 ± 0.0
Gly
12.907GlyAla: 12.907 ± 2.574
0.182GlyCys: 0.182 ± 0.186
4.545GlyAsp: 4.545 ± 1.087
3.636GlyGlu: 3.636 ± 0.848
2.727GlyPhe: 2.727 ± 0.487
6.726GlyGly: 6.726 ± 1.437
1.272GlyHis: 1.272 ± 0.451
5.454GlyIle: 5.454 ± 1.016
2.727GlyLys: 2.727 ± 0.658
7.635GlyLeu: 7.635 ± 2.298
1.636GlyMet: 1.636 ± 0.355
2.545GlyAsn: 2.545 ± 0.625
2.545GlyPro: 2.545 ± 0.927
3.636GlyGln: 3.636 ± 0.738
6.181GlyArg: 6.181 ± 0.843
7.271GlySer: 7.271 ± 1.079
4.363GlyThr: 4.363 ± 0.767
9.635GlyVal: 9.635 ± 1.185
2.363GlyTrp: 2.363 ± 0.603
2.0GlyTyr: 2.0 ± 0.486
0.0GlyXaa: 0.0 ± 0.0
His
1.272HisAla: 1.272 ± 0.407
0.0HisCys: 0.0 ± 0.0
1.272HisAsp: 1.272 ± 0.578
0.364HisGlu: 0.364 ± 0.274
0.545HisPhe: 0.545 ± 0.307
1.454HisGly: 1.454 ± 0.589
0.182HisHis: 0.182 ± 0.191
0.364HisIle: 0.364 ± 0.222
0.364HisLys: 0.364 ± 0.304
1.091HisLeu: 1.091 ± 0.36
0.182HisMet: 0.182 ± 0.139
0.364HisAsn: 0.364 ± 0.235
0.909HisPro: 0.909 ± 0.367
0.182HisGln: 0.182 ± 0.18
1.454HisArg: 1.454 ± 0.57
0.727HisSer: 0.727 ± 0.32
1.091HisThr: 1.091 ± 0.513
2.181HisVal: 2.181 ± 0.597
0.364HisTrp: 0.364 ± 0.186
0.727HisTyr: 0.727 ± 0.299
0.0HisXaa: 0.0 ± 0.0
Ile
4.363IleAla: 4.363 ± 0.777
0.182IleCys: 0.182 ± 0.173
3.454IleAsp: 3.454 ± 0.769
5.999IleGlu: 5.999 ± 0.997
0.727IlePhe: 0.727 ± 0.294
3.454IleGly: 3.454 ± 0.757
1.454IleHis: 1.454 ± 0.438
0.909IleIle: 0.909 ± 0.501
0.545IleLys: 0.545 ± 0.286
3.454IleLeu: 3.454 ± 1.276
0.182IleMet: 0.182 ± 0.193
0.909IleAsn: 0.909 ± 0.453
2.909IlePro: 2.909 ± 0.984
1.091IleGln: 1.091 ± 0.438
3.636IleArg: 3.636 ± 0.619
3.999IleSer: 3.999 ± 0.886
2.909IleThr: 2.909 ± 0.661
3.999IleVal: 3.999 ± 0.888
0.364IleTrp: 0.364 ± 0.222
0.364IleTyr: 0.364 ± 0.246
0.0IleXaa: 0.0 ± 0.0
Lys
1.636LysAla: 1.636 ± 0.424
0.0LysCys: 0.0 ± 0.0
0.909LysAsp: 0.909 ± 0.372
0.364LysGlu: 0.364 ± 0.247
0.909LysPhe: 0.909 ± 0.574
1.091LysGly: 1.091 ± 0.459
0.364LysHis: 0.364 ± 0.234
1.454LysIle: 1.454 ± 0.549
0.545LysLys: 0.545 ± 0.292
2.0LysLeu: 2.0 ± 0.621
0.545LysMet: 0.545 ± 0.279
0.909LysAsn: 0.909 ± 0.526
0.545LysPro: 0.545 ± 0.35
0.364LysGln: 0.364 ± 0.247
2.181LysArg: 2.181 ± 0.924
2.363LysSer: 2.363 ± 0.449
2.181LysThr: 2.181 ± 0.602
0.545LysVal: 0.545 ± 0.453
0.545LysTrp: 0.545 ± 0.264
0.364LysTyr: 0.364 ± 0.186
0.0LysXaa: 0.0 ± 0.0
Leu
9.998LeuAla: 9.998 ± 1.259
0.545LeuCys: 0.545 ± 0.272
6.908LeuAsp: 6.908 ± 1.409
2.181LeuGlu: 2.181 ± 0.476
3.817LeuPhe: 3.817 ± 0.88
8.362LeuGly: 8.362 ± 1.115
1.636LeuHis: 1.636 ± 0.428
4.908LeuIle: 4.908 ± 1.206
0.909LeuLys: 0.909 ± 0.342
7.271LeuLeu: 7.271 ± 2.076
2.363LeuMet: 2.363 ± 0.615
3.817LeuAsn: 3.817 ± 0.95
7.999LeuPro: 7.999 ± 1.467
5.817LeuGln: 5.817 ± 0.981
6.544LeuArg: 6.544 ± 0.935
5.454LeuSer: 5.454 ± 0.897
8.544LeuThr: 8.544 ± 1.014
5.999LeuVal: 5.999 ± 1.291
0.545LeuTrp: 0.545 ± 0.316
1.818LeuTyr: 1.818 ± 0.595
0.0LeuXaa: 0.0 ± 0.0
Met
2.909MetAla: 2.909 ± 0.67
0.0MetCys: 0.0 ± 0.0
1.272MetAsp: 1.272 ± 0.376
0.727MetGlu: 0.727 ± 0.331
0.545MetPhe: 0.545 ± 0.255
2.181MetGly: 2.181 ± 0.658
0.364MetHis: 0.364 ± 0.226
1.454MetIle: 1.454 ± 0.785
0.182MetLys: 0.182 ± 0.18
0.545MetLeu: 0.545 ± 0.356
0.0MetMet: 0.0 ± 0.0
1.091MetAsn: 1.091 ± 0.406
1.091MetPro: 1.091 ± 0.367
0.364MetGln: 0.364 ± 0.318
1.454MetArg: 1.454 ± 0.64
2.363MetSer: 2.363 ± 1.071
1.818MetThr: 1.818 ± 0.62
1.272MetVal: 1.272 ± 0.473
0.182MetTrp: 0.182 ± 0.18
0.182MetTyr: 0.182 ± 0.175
0.0MetXaa: 0.0 ± 0.0
Asn
6.908AsnAla: 6.908 ± 0.893
0.182AsnCys: 0.182 ± 0.18
0.364AsnAsp: 0.364 ± 0.221
0.909AsnGlu: 0.909 ± 0.341
0.545AsnPhe: 0.545 ± 0.3
3.817AsnGly: 3.817 ± 0.636
0.727AsnHis: 0.727 ± 0.35
0.909AsnIle: 0.909 ± 0.402
0.909AsnLys: 0.909 ± 0.339
2.727AsnLeu: 2.727 ± 0.859
0.545AsnMet: 0.545 ± 0.264
0.909AsnAsn: 0.909 ± 0.491
1.454AsnPro: 1.454 ± 0.381
0.0AsnGln: 0.0 ± 0.0
2.181AsnArg: 2.181 ± 0.598
2.363AsnSer: 2.363 ± 0.528
1.272AsnThr: 1.272 ± 0.369
2.363AsnVal: 2.363 ± 0.62
0.0AsnTrp: 0.0 ± 0.0
0.545AsnTyr: 0.545 ± 0.294
0.0AsnXaa: 0.0 ± 0.0
Pro
9.453ProAla: 9.453 ± 1.451
0.364ProCys: 0.364 ± 0.263
2.363ProAsp: 2.363 ± 0.789
2.909ProGlu: 2.909 ± 0.891
1.091ProPhe: 1.091 ± 0.403
3.999ProGly: 3.999 ± 0.777
0.727ProHis: 0.727 ± 0.393
2.0ProIle: 2.0 ± 0.459
1.818ProLys: 1.818 ± 0.616
4.181ProLeu: 4.181 ± 0.892
1.091ProMet: 1.091 ± 0.578
1.454ProAsn: 1.454 ± 0.554
1.636ProPro: 1.636 ± 0.77
2.181ProGln: 2.181 ± 0.617
3.09ProArg: 3.09 ± 0.786
3.272ProSer: 3.272 ± 0.468
3.817ProThr: 3.817 ± 0.959
3.817ProVal: 3.817 ± 0.841
0.364ProTrp: 0.364 ± 0.245
1.454ProTyr: 1.454 ± 0.467
0.0ProXaa: 0.0 ± 0.0
Gln
4.545GlnAla: 4.545 ± 0.949
0.0GlnCys: 0.0 ± 0.0
1.454GlnAsp: 1.454 ± 0.439
0.909GlnGlu: 0.909 ± 0.262
1.818GlnPhe: 1.818 ± 0.466
2.545GlnGly: 2.545 ± 0.659
1.091GlnHis: 1.091 ± 0.461
1.818GlnIle: 1.818 ± 0.441
0.545GlnLys: 0.545 ± 0.304
9.998GlnLeu: 9.998 ± 1.455
0.727GlnMet: 0.727 ± 0.498
1.454GlnAsn: 1.454 ± 0.463
3.09GlnPro: 3.09 ± 1.002
2.727GlnGln: 2.727 ± 0.596
3.454GlnArg: 3.454 ± 0.766
1.636GlnSer: 1.636 ± 0.481
1.272GlnThr: 1.272 ± 0.492
1.636GlnVal: 1.636 ± 0.465
0.182GlnTrp: 0.182 ± 0.159
0.727GlnTyr: 0.727 ± 0.372
0.0GlnXaa: 0.0 ± 0.0
Arg
9.089ArgAla: 9.089 ± 1.606
0.727ArgCys: 0.727 ± 0.369
5.454ArgAsp: 5.454 ± 0.882
6.362ArgGlu: 6.362 ± 1.48
2.363ArgPhe: 2.363 ± 0.617
4.726ArgGly: 4.726 ± 0.812
0.909ArgHis: 0.909 ± 0.433
3.09ArgIle: 3.09 ± 0.801
1.272ArgLys: 1.272 ± 0.43
6.908ArgLeu: 6.908 ± 1.143
1.272ArgMet: 1.272 ± 0.469
1.636ArgAsn: 1.636 ± 0.481
2.545ArgPro: 2.545 ± 0.869
3.999ArgGln: 3.999 ± 0.993
8.544ArgArg: 8.544 ± 2.048
2.545ArgSer: 2.545 ± 0.615
2.545ArgThr: 2.545 ± 0.617
5.999ArgVal: 5.999 ± 1.104
2.0ArgTrp: 2.0 ± 0.569
2.0ArgTyr: 2.0 ± 0.516
0.0ArgXaa: 0.0 ± 0.0
Ser
9.453SerAla: 9.453 ± 0.991
0.364SerCys: 0.364 ± 0.346
2.363SerAsp: 2.363 ± 0.589
3.636SerGlu: 3.636 ± 0.89
1.091SerPhe: 1.091 ± 0.339
3.999SerGly: 3.999 ± 0.771
1.272SerHis: 1.272 ± 0.448
3.272SerIle: 3.272 ± 0.807
1.091SerLys: 1.091 ± 0.413
3.999SerLeu: 3.999 ± 1.001
2.181SerMet: 2.181 ± 0.679
1.636SerAsn: 1.636 ± 0.588
2.0SerPro: 2.0 ± 0.457
2.727SerGln: 2.727 ± 0.575
3.817SerArg: 3.817 ± 0.775
4.545SerSer: 4.545 ± 0.909
4.181SerThr: 4.181 ± 0.824
4.363SerVal: 4.363 ± 0.611
1.091SerTrp: 1.091 ± 0.427
1.454SerTyr: 1.454 ± 0.562
0.0SerXaa: 0.0 ± 0.0
Thr
8.726ThrAla: 8.726 ± 1.609
0.0ThrCys: 0.0 ± 0.0
2.545ThrAsp: 2.545 ± 0.755
3.817ThrGlu: 3.817 ± 1.078
4.181ThrPhe: 4.181 ± 0.878
7.09ThrGly: 7.09 ± 1.65
0.909ThrHis: 0.909 ± 0.461
3.272ThrIle: 3.272 ± 1.318
1.272ThrLys: 1.272 ± 0.465
4.908ThrLeu: 4.908 ± 1.029
1.818ThrMet: 1.818 ± 0.581
1.454ThrAsn: 1.454 ± 0.437
4.363ThrPro: 4.363 ± 0.802
1.091ThrGln: 1.091 ± 0.496
4.363ThrArg: 4.363 ± 0.926
2.545ThrSer: 2.545 ± 0.682
5.635ThrThr: 5.635 ± 1.132
5.817ThrVal: 5.817 ± 1.091
1.091ThrTrp: 1.091 ± 0.442
1.091ThrTyr: 1.091 ± 0.5
0.0ThrXaa: 0.0 ± 0.0
Val
11.089ValAla: 11.089 ± 1.436
0.0ValCys: 0.0 ± 0.0
3.09ValAsp: 3.09 ± 0.639
5.272ValGlu: 5.272 ± 1.242
1.272ValPhe: 1.272 ± 0.578
6.181ValGly: 6.181 ± 1.239
0.545ValHis: 0.545 ± 0.293
3.454ValIle: 3.454 ± 0.989
1.272ValLys: 1.272 ± 0.5
4.545ValLeu: 4.545 ± 1.036
1.091ValMet: 1.091 ± 0.646
2.181ValAsn: 2.181 ± 0.918
3.999ValPro: 3.999 ± 0.788
4.726ValGln: 4.726 ± 1.205
6.181ValArg: 6.181 ± 1.225
5.817ValSer: 5.817 ± 1.006
6.362ValThr: 6.362 ± 1.109
6.544ValVal: 6.544 ± 1.439
0.545ValTrp: 0.545 ± 0.326
1.454ValTyr: 1.454 ± 0.38
0.0ValXaa: 0.0 ± 0.0
Trp
1.636TrpAla: 1.636 ± 0.481
0.0TrpCys: 0.0 ± 0.0
0.545TrpAsp: 0.545 ± 0.266
0.727TrpGlu: 0.727 ± 0.451
1.091TrpPhe: 1.091 ± 0.343
0.727TrpGly: 0.727 ± 0.26
0.727TrpHis: 0.727 ± 0.374
1.272TrpIle: 1.272 ± 0.424
0.0TrpLys: 0.0 ± 0.0
2.545TrpLeu: 2.545 ± 0.795
0.182TrpMet: 0.182 ± 0.175
0.364TrpAsn: 0.364 ± 0.186
0.364TrpPro: 0.364 ± 0.359
0.545TrpGln: 0.545 ± 0.301
0.545TrpArg: 0.545 ± 0.291
0.727TrpSer: 0.727 ± 0.302
1.454TrpThr: 1.454 ± 0.538
0.364TrpVal: 0.364 ± 0.233
0.0TrpTrp: 0.0 ± 0.0
0.364TrpTyr: 0.364 ± 0.208
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.181TyrAla: 2.181 ± 0.593
0.364TyrCys: 0.364 ± 0.217
0.909TyrAsp: 0.909 ± 0.367
1.636TyrGlu: 1.636 ± 0.41
0.545TyrPhe: 0.545 ± 0.285
3.272TyrGly: 3.272 ± 0.708
0.0TyrHis: 0.0 ± 0.0
0.364TyrIle: 0.364 ± 0.279
0.545TyrLys: 0.545 ± 0.238
0.545TyrLeu: 0.545 ± 0.283
0.727TyrMet: 0.727 ± 0.415
0.364TyrAsn: 0.364 ± 0.241
1.818TyrPro: 1.818 ± 0.531
1.272TyrGln: 1.272 ± 0.381
2.181TyrArg: 2.181 ± 0.719
0.727TyrSer: 0.727 ± 0.34
0.545TyrThr: 0.545 ± 0.291
2.909TyrVal: 2.909 ± 0.75
0.364TyrTrp: 0.364 ± 0.279
0.727TyrTyr: 0.727 ± 0.393
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 25 proteins (5502 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski