Streptococcus phage Javan78

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BEV9|A0A4D6BEV9_9CAUD Minor capsid protein OS=Streptococcus phage Javan78 OX=2548306 GN=Javan78_0011 PE=4 SV=1
MM1 pKa = 7.51NKK3 pKa = 9.68NDD5 pKa = 4.12LFVDD9 pKa = 4.34VASHH13 pKa = 5.34QGYY16 pKa = 10.07NITGILGQMGTTNTIIKK33 pKa = 9.7ISEE36 pKa = 3.99STSYY40 pKa = 11.02INPCLSAQVEE50 pKa = 4.11QSNPIGFYY58 pKa = 9.88HH59 pKa = 5.76YY60 pKa = 10.64AWFGGDD66 pKa = 2.96VAEE69 pKa = 4.77AEE71 pKa = 3.99RR72 pKa = 11.84EE73 pKa = 3.78ARR75 pKa = 11.84YY76 pKa = 9.49FIDD79 pKa = 4.55NVPQKK84 pKa = 11.01VKK86 pKa = 10.38YY87 pKa = 10.42LCLDD91 pKa = 3.96YY92 pKa = 10.92EE93 pKa = 4.44DD94 pKa = 5.0HH95 pKa = 7.98ASGDD99 pKa = 3.59KK100 pKa = 10.11QANTDD105 pKa = 2.83ACLRR109 pKa = 11.84FMEE112 pKa = 4.24ILKK115 pKa = 10.54EE116 pKa = 3.95NGYY119 pKa = 10.25EE120 pKa = 4.01PIYY123 pKa = 10.7YY124 pKa = 9.76SYY126 pKa = 11.64KK127 pKa = 10.54PFTLNNIYY135 pKa = 10.45YY136 pKa = 8.43EE137 pKa = 4.23QILARR142 pKa = 11.84FPNSLWIAGYY152 pKa = 10.38GLNDD156 pKa = 3.33GNADD160 pKa = 3.61FNYY163 pKa = 8.87FPSMDD168 pKa = 4.52GIRR171 pKa = 11.84WWQYY175 pKa = 10.03SSNPFDD181 pKa = 4.42KK182 pKa = 11.23NIVLLDD188 pKa = 3.94DD189 pKa = 4.18EE190 pKa = 5.68EE191 pKa = 4.64EE192 pKa = 4.62DD193 pKa = 4.11NVISKK198 pKa = 10.66DD199 pKa = 3.49AVKK202 pKa = 10.71SLNTVADD209 pKa = 3.93EE210 pKa = 4.71VIQGLWGNGQEE221 pKa = 5.08RR222 pKa = 11.84FDD224 pKa = 3.84NLTNAGYY231 pKa = 9.79NAQAVQDD238 pKa = 4.13KK239 pKa = 11.44VNDD242 pKa = 3.94LLNAEE247 pKa = 4.45NTSKK251 pKa = 11.19DD252 pKa = 3.75LDD254 pKa = 3.81TLANEE259 pKa = 4.39VLQGLWGNGQEE270 pKa = 5.14RR271 pKa = 11.84FDD273 pKa = 3.71KK274 pKa = 11.2LEE276 pKa = 3.98NAGYY280 pKa = 9.79DD281 pKa = 3.43AQAVQDD287 pKa = 4.1RR288 pKa = 11.84VNSLLSGGNTADD300 pKa = 4.36LDD302 pKa = 4.12TVANEE307 pKa = 4.18VVQGLWGNGQEE318 pKa = 5.08RR319 pKa = 11.84FDD321 pKa = 3.84NLTNAGYY328 pKa = 9.89DD329 pKa = 3.29AQAVQNRR336 pKa = 11.84VNEE339 pKa = 4.16LLSS342 pKa = 3.58

Molecular weight: 38.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BEH9|A0A4D6BEH9_9CAUD Terminase small subunit OS=Streptococcus phage Javan78 OX=2548306 GN=Javan78_0022 PE=4 SV=1
MM1 pKa = 7.72ADD3 pKa = 3.16VRR5 pKa = 11.84VSIDD9 pKa = 3.26LAGVEE14 pKa = 4.86KK15 pKa = 10.52KK16 pKa = 10.72VSPQAMQRR24 pKa = 11.84GKK26 pKa = 9.98IAAGSEE32 pKa = 3.87ALLIMDD38 pKa = 4.07SSVPLRR44 pKa = 11.84AGGGALRR51 pKa = 11.84ASGRR55 pKa = 11.84VEE57 pKa = 3.73PNGNVSYY64 pKa = 9.14NTVYY68 pKa = 10.96ARR70 pKa = 11.84AQFHH74 pKa = 5.45GTNGIVVFRR83 pKa = 11.84KK84 pKa = 8.49YY85 pKa = 6.16TTSGTGSRR93 pKa = 11.84WDD95 pKa = 3.86KK96 pKa = 10.38PLKK99 pKa = 10.93ANIDD103 pKa = 3.85KK104 pKa = 10.78LKK106 pKa = 10.34KK107 pKa = 10.11AAIKK111 pKa = 10.82GMGIRR116 pKa = 4.21

Molecular weight: 12.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

11218

48

1293

211.7

23.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.247 ± 1.036

0.526 ± 0.106

6.276 ± 0.423

6.864 ± 0.537

4.172 ± 0.218

6.605 ± 0.585

1.373 ± 0.14

7.31 ± 0.232

8.121 ± 0.457

8.388 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.469 ± 0.261

6.106 ± 0.345

2.549 ± 0.182

4.511 ± 0.236

3.601 ± 0.382

6.4 ± 0.465

5.999 ± 0.237

6.463 ± 0.167

1.007 ± 0.133

4.011 ± 0.414
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski