Powellomyces hirtus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Chytridiomycota; Chytridiomycota incertae sedis; Chytridiomycetes; Spizellomycetales; Powellomycetaceae; Powellomyces

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6533 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A507DR57|A0A507DR57_9FUNG Uncharacterized protein OS=Powellomyces hirtus OX=109895 GN=PhCBS80983_g06311 PE=4 SV=1
MM1 pKa = 7.27NAVSSQTYY9 pKa = 9.64NVTYY13 pKa = 10.72DD14 pKa = 3.54NLSGKK19 pKa = 8.26ITVDD23 pKa = 3.25SSTTPFKK30 pKa = 10.79FFQNGSTSQKK40 pKa = 10.41ILGLTGDD47 pKa = 4.24TPSSSSVTFQNPIDD61 pKa = 3.81LTGVKK66 pKa = 8.78MVLVTSQNVCSNNVVVAGQEE86 pKa = 4.09SLNILACIPINQEE99 pKa = 3.9TQTVLCSQNYY109 pKa = 9.16FDD111 pKa = 6.18SDD113 pKa = 4.19FIDD116 pKa = 3.86TEE118 pKa = 4.34SGLVSTVDD126 pKa = 3.11IQLLDD131 pKa = 3.87SEE133 pKa = 4.93TLLPLDD139 pKa = 4.41LKK141 pKa = 11.23GKK143 pKa = 10.46AFTLVIDD150 pKa = 3.99CHH152 pKa = 8.11SSGFTDD158 pKa = 3.38

Molecular weight: 16.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A507E096|A0A507E096_9FUNG Uncharacterized protein OS=Powellomyces hirtus OX=109895 GN=PhCBS80983_g04517 PE=4 SV=1
MM1 pKa = 7.6SNLLRR6 pKa = 11.84DD7 pKa = 3.62HH8 pKa = 6.87FKK10 pKa = 11.24EE11 pKa = 5.15KK12 pKa = 9.62GTQHH16 pKa = 5.58QRR18 pKa = 11.84SSVNTPQQNGVAEE31 pKa = 4.24RR32 pKa = 11.84ANRR35 pKa = 11.84TLVEE39 pKa = 4.08MGRR42 pKa = 11.84CLLKK46 pKa = 10.79QSGLDD51 pKa = 3.22KK52 pKa = 10.86RR53 pKa = 11.84FWGEE57 pKa = 3.91AIMTAVYY64 pKa = 10.2LRR66 pKa = 11.84NRR68 pKa = 11.84SPTVACPKK76 pKa = 9.43MPYY79 pKa = 10.65EE80 pKa = 4.35MFHH83 pKa = 7.08GIPPSLGRR91 pKa = 11.84LDD93 pKa = 4.71ALPMPTFNPTSARR106 pKa = 11.84SWMMLPSSASCWDD119 pKa = 3.29IAPTARR125 pKa = 11.84NTGLWTYY132 pKa = 10.35RR133 pKa = 11.84LRR135 pKa = 11.84RR136 pKa = 11.84SCSPGVSNHH145 pKa = 6.22PSSLLRR151 pKa = 11.84CAKK154 pKa = 9.83GLRR157 pKa = 11.84RR158 pKa = 11.84APKK161 pKa = 9.48WEE163 pKa = 3.85LAA165 pKa = 3.63

Molecular weight: 18.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6533

0

6533

3660536

9

12122

560.3

61.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.201 ± 0.031

1.199 ± 0.009

5.742 ± 0.02

6.109 ± 0.029

3.584 ± 0.016

6.394 ± 0.023

2.542 ± 0.014

4.8 ± 0.018

5.243 ± 0.03

9.038 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.317 ± 0.009

3.665 ± 0.014

5.879 ± 0.029

4.089 ± 0.024

5.888 ± 0.021

7.65 ± 0.032

6.264 ± 0.02

6.557 ± 0.025

1.177 ± 0.009

2.661 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski