Thermodesulfobacterium geofontis (strain OPF15)

Taxonomy: cellular organisms; Bacteria; Thermodesulfobacteria; Thermodesulfobacteria; Thermodesulfobacteriales; Thermodesulfobacteriaceae; Thermodesulfobacterium; Thermodesulfobacterium geofontis

Average proteome isoelectric point is 7.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1594 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8C5M8|F8C5M8_THEGP Uncharacterized protein OS=Thermodesulfobacterium geofontis (strain OPF15) OX=795359 GN=TOPB45_0911 PE=4 SV=1
MM1 pKa = 7.47LKK3 pKa = 10.31KK4 pKa = 10.4LAVGLGCLGLLLGATTMSYY23 pKa = 10.99AHH25 pKa = 7.0GGNSGNGGGCSGDD38 pKa = 3.27ACQVSHH44 pKa = 6.84AVDD47 pKa = 3.36VEE49 pKa = 4.2LGVNVKK55 pKa = 10.54LDD57 pKa = 3.46VDD59 pKa = 3.97VCIDD63 pKa = 3.71AGNMNDD69 pKa = 5.45DD70 pKa = 3.81FWSTQASIGQYY81 pKa = 10.29GDD83 pKa = 3.98EE84 pKa = 4.2NTASITQTSTSQLAGILQVGDD105 pKa = 3.48QNKK108 pKa = 10.61AGISQTGNNEE118 pKa = 3.54YY119 pKa = 10.81AAIGQYY125 pKa = 11.26GDD127 pKa = 4.59LNSAYY132 pKa = 9.69INQTLQNAAAITIQWGNSNIASISQQ157 pKa = 3.1

Molecular weight: 16.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8C4N9|F8C4N9_THEGP RNA 2' 3'-cyclic phosphodiesterase OS=Thermodesulfobacterium geofontis (strain OPF15) OX=795359 GN=TOPB45_0605 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.85QPSRR9 pKa = 11.84IKK11 pKa = 9.73MKK13 pKa = 10.02RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 5.06GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.32TRR25 pKa = 11.84SGRR28 pKa = 11.84RR29 pKa = 11.84ILRR32 pKa = 11.84NRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.63GRR39 pKa = 11.84WRR41 pKa = 11.84LTVV44 pKa = 3.0

Molecular weight: 5.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1594

0

1594

506494

30

2281

317.8

36.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.484 ± 0.057

1.025 ± 0.024

4.127 ± 0.031

8.947 ± 0.081

5.664 ± 0.07

6.123 ± 0.061

1.391 ± 0.025

9.525 ± 0.06

10.28 ± 0.081

11.118 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.625 ± 0.022

4.221 ± 0.062

4.236 ± 0.042

2.21 ± 0.025

3.775 ± 0.044

5.225 ± 0.047

4.201 ± 0.044

5.727 ± 0.051

0.965 ± 0.02

4.132 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski