Streptomyces sp. A7024

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8226 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G4TU32|A0A6G4TU32_9ACTN PHB depolymerase family esterase OS=Streptomyces sp. A7024 OX=1128680 GN=G5C51_01640 PE=4 SV=1
MM1 pKa = 7.81GGPLIAVPVSALSQWHH17 pKa = 6.63GCTQSGMVVGSDD29 pKa = 5.33DD30 pKa = 5.87DD31 pKa = 5.43LDD33 pKa = 5.65DD34 pKa = 4.05YY35 pKa = 12.03DD36 pKa = 4.21RR37 pKa = 11.84ACEE40 pKa = 4.06VEE42 pKa = 4.37GLAGVIAVGADD53 pKa = 3.48GAQGLVLADD62 pKa = 4.2EE63 pKa = 4.95PASSFYY69 pKa = 11.0LPEE72 pKa = 4.34HH73 pKa = 6.26RR74 pKa = 11.84AFVRR78 pKa = 11.84WLGANSEE85 pKa = 4.38DD86 pKa = 5.19DD87 pKa = 3.97LLAAARR93 pKa = 11.84AVLADD98 pKa = 3.64STAMWEE104 pKa = 4.23EE105 pKa = 4.07CGIWQTDD112 pKa = 3.93GPAVLMDD119 pKa = 4.08SVTAGAEE126 pKa = 4.08VSVGYY131 pKa = 9.73PNGAGMPEE139 pKa = 4.09QAPVALPPGSWMVRR153 pKa = 11.84AVHH156 pKa = 6.7ASPIEE161 pKa = 4.1DD162 pKa = 3.23TSVGVIQLLPQPSSS176 pKa = 3.12

Molecular weight: 18.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G4TW08|A0A6G4TW08_9ACTN Dihydrodipicolinate synthase family protein OS=Streptomyces sp. A7024 OX=1128680 GN=G5C51_09160 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 11.06GRR40 pKa = 11.84GRR42 pKa = 11.84LSAA45 pKa = 4.4

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8226

0

8226

2696956

23

3069

327.9

35.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.018 ± 0.043

0.747 ± 0.007

6.026 ± 0.022

5.761 ± 0.03

2.774 ± 0.017

9.638 ± 0.026

2.187 ± 0.012

3.37 ± 0.018

2.478 ± 0.03

10.352 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.784 ± 0.011

1.784 ± 0.014

5.991 ± 0.023

2.802 ± 0.013

7.77 ± 0.03

4.818 ± 0.018

5.886 ± 0.019

8.05 ± 0.021

1.546 ± 0.012

2.219 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski