Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio; Vibrio cholerae; Vibrio cholerae O1; Vibrio cholerae O1 biovar El Tor

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3782 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9KRA3|FABV1_VIBCH Enoyl-[acyl-carrier-protein] reductase [NADH] 1 OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=fabV PE=1 SV=2
MM1 pKa = 6.75QTLQEE6 pKa = 4.25ILQQPEE12 pKa = 4.61LEE14 pKa = 4.2NKK16 pKa = 9.92LLNQAKK22 pKa = 8.09TVGFVTAMACCPNVLPPEE40 pKa = 4.04EE41 pKa = 4.37WLPFLWGGEE50 pKa = 4.1EE51 pKa = 3.76IAPFSDD57 pKa = 5.12PIQLEE62 pKa = 4.09NYY64 pKa = 8.93FEE66 pKa = 5.21HH67 pKa = 7.91IIALWNEE74 pKa = 3.91TRR76 pKa = 11.84SQLLEE81 pKa = 5.08GNWQWPTGYY90 pKa = 10.65LLDD93 pKa = 3.87EE94 pKa = 4.63AEE96 pKa = 4.32IVNQEE101 pKa = 3.65VRR103 pKa = 11.84DD104 pKa = 4.07FCEE107 pKa = 4.71GMLQGWQLARR117 pKa = 11.84DD118 pKa = 3.88DD119 pKa = 4.58WEE121 pKa = 4.11VLMPEE126 pKa = 4.0NSSDD130 pKa = 3.42NTLLGGVLLSLSMLYY145 pKa = 10.61DD146 pKa = 3.89PEE148 pKa = 4.25TSIATLYY155 pKa = 9.63EE156 pKa = 3.79QGFTGLEE163 pKa = 3.89QFAEE167 pKa = 4.62IYY169 pKa = 10.21HH170 pKa = 7.3AIPVMLCGITQRR182 pKa = 11.84GVTLAEE188 pKa = 4.1AQQ190 pKa = 3.39

Molecular weight: 21.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9KVY2|RNPA_VIBCH Ribonuclease P protein component OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=rnpA PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 8.98RR4 pKa = 11.84TFQPSVLKK12 pKa = 10.49RR13 pKa = 11.84KK14 pKa = 7.97RR15 pKa = 11.84THH17 pKa = 5.89GFRR20 pKa = 11.84ARR22 pKa = 11.84MATANGRR29 pKa = 11.84KK30 pKa = 9.15VLNARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.78GRR40 pKa = 11.84KK41 pKa = 8.91RR42 pKa = 11.84LSKK45 pKa = 10.84

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3782

0

3782

1153695

26

4558

305.0

33.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.167 ± 0.045

1.048 ± 0.015

5.023 ± 0.035

6.206 ± 0.041

4.071 ± 0.028

6.698 ± 0.042

2.397 ± 0.024

6.045 ± 0.031

4.919 ± 0.035

10.853 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.72 ± 0.02

3.898 ± 0.028

4.021 ± 0.026

5.183 ± 0.038

4.934 ± 0.037

6.319 ± 0.031

5.189 ± 0.026

7.033 ± 0.038

1.318 ± 0.016

2.959 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski