Amino acid dipepetide frequency for Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.542AlaAla: 8.542 ± 0.133	0.989AlaCys: 0.989 ± 0.03	4.503AlaAsp: 4.503 ± 0.067	5.819AlaGlu: 5.819 ± 0.087	3.47AlaPhe: 3.47 ± 0.061	6.093AlaGly: 6.093 ± 0.092	1.907AlaHis: 1.907 ± 0.041	5.858AlaIle: 5.858 ± 0.083	4.966AlaLys: 4.966 ± 0.065	10.918AlaLeu: 10.918 ± 0.119	2.991AlaMet: 2.991 ± 0.051	3.535AlaAsn: 3.535 ± 0.06	3.061AlaPro: 3.061 ± 0.054	5.329AlaGln: 5.329 ± 0.078	4.183AlaArg: 4.183 ± 0.067	5.328AlaSer: 5.328 ± 0.065	4.561AlaThr: 4.561 ± 0.063	6.051AlaVal: 6.051 ± 0.083	1.12AlaTrp: 1.12 ± 0.035	2.45AlaTyr: 2.45 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	0.899CysAla: 0.899 ± 0.028	0.168CysCys: 0.168 ± 0.011	0.608CysAsp: 0.608 ± 0.026	0.614CysGlu: 0.614 ± 0.027	0.479CysPhe: 0.479 ± 0.02	0.94CysGly: 0.94 ± 0.033	0.357CysHis: 0.357 ± 0.019	0.543CysIle: 0.543 ± 0.02	0.4CysLys: 0.4 ± 0.019	1.031CysLeu: 1.031 ± 0.037	0.241CysMet: 0.241 ± 0.015	0.344CysAsn: 0.344 ± 0.02	0.455CysPro: 0.455 ± 0.022	0.561CysGln: 0.561 ± 0.024	0.502CysArg: 0.502 ± 0.021	0.68CysSer: 0.68 ± 0.028	0.466CysThr: 0.466 ± 0.02	0.71CysVal: 0.71 ± 0.027	0.148CysTrp: 0.148 ± 0.011	0.329CysTyr: 0.329 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	4.369AspAla: 4.369 ± 0.067	0.518AspCys: 0.518 ± 0.023	2.485AspAsp: 2.485 ± 0.055	3.593AspGlu: 3.593 ± 0.066	2.316AspPhe: 2.316 ± 0.049	3.331AspGly: 3.331 ± 0.063	1.117AspHis: 1.117 ± 0.032	3.35AspIle: 3.35 ± 0.061	2.826AspLys: 2.826 ± 0.051	5.1AspLeu: 5.1 ± 0.073	1.314AspMet: 1.314 ± 0.036	2.036AspAsn: 2.036 ± 0.047	2.068AspPro: 2.068 ± 0.045	1.952AspGln: 1.952 ± 0.043	2.281AspArg: 2.281 ± 0.043	2.85AspSer: 2.85 ± 0.053	2.278AspThr: 2.278 ± 0.049	3.705AspVal: 3.705 ± 0.055	0.878AspTrp: 0.878 ± 0.029	1.864AspTyr: 1.864 ± 0.046	0.001AspXaa: 0.001 ± 0.001
Glu	5.338GluAla: 5.338 ± 0.071	0.552GluCys: 0.552 ± 0.021	2.264GluAsp: 2.264 ± 0.052	3.69GluGlu: 3.69 ± 0.074	2.463GluPhe: 2.463 ± 0.048	3.448GluGly: 3.448 ± 0.057	1.635GluHis: 1.635 ± 0.044	3.772GluIle: 3.772 ± 0.053	3.569GluLys: 3.569 ± 0.062	7.297GluLeu: 7.297 ± 0.103	1.823GluMet: 1.823 ± 0.044	2.366GluAsn: 2.366 ± 0.048	2.176GluPro: 2.176 ± 0.051	4.597GluGln: 4.597 ± 0.077	3.766GluArg: 3.766 ± 0.067	3.531GluSer: 3.531 ± 0.061	2.941GluThr: 2.941 ± 0.043	4.264GluVal: 4.264 ± 0.067	0.901GluTrp: 0.901 ± 0.026	1.66GluTyr: 1.66 ± 0.042	0.001GluXaa: 0.001 ± 0.001
Phe	4.037PheAla: 4.037 ± 0.063	0.53PheCys: 0.53 ± 0.023	2.518PheAsp: 2.518 ± 0.051	2.398PheGlu: 2.398 ± 0.046	1.669PhePhe: 1.669 ± 0.048	3.168PheGly: 3.168 ± 0.058	0.914PheHis: 0.914 ± 0.027	2.633PheIle: 2.633 ± 0.057	1.802PheLys: 1.802 ± 0.038	3.51PheLeu: 3.51 ± 0.057	1.073PheMet: 1.073 ± 0.031	1.83PheAsn: 1.83 ± 0.043	1.449PhePro: 1.449 ± 0.034	1.359PheGln: 1.359 ± 0.033	1.581PheArg: 1.581 ± 0.04	3.217PheSer: 3.217 ± 0.056	2.371PheThr: 2.371 ± 0.045	2.758PheVal: 2.758 ± 0.051	0.573PheTrp: 0.573 ± 0.027	1.318PheTyr: 1.318 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	5.228GlyAla: 5.228 ± 0.089	0.868GlyCys: 0.868 ± 0.03	3.327GlyAsp: 3.327 ± 0.076	4.384GlyGlu: 4.384 ± 0.064	3.291GlyPhe: 3.291 ± 0.058	4.546GlyGly: 4.546 ± 0.079	1.591GlyHis: 1.591 ± 0.039	4.693GlyIle: 4.693 ± 0.069	3.855GlyLys: 3.855 ± 0.063	7.036GlyLeu: 7.036 ± 0.094	2.062GlyMet: 2.062 ± 0.041	2.381GlyAsn: 2.381 ± 0.067	1.527GlyPro: 1.527 ± 0.037	3.05GlyGln: 3.05 ± 0.057	3.244GlyArg: 3.244 ± 0.055	3.99GlySer: 3.99 ± 0.064	3.207GlyThr: 3.207 ± 0.054	5.189GlyVal: 5.189 ± 0.082	0.992GlyTrp: 0.992 ± 0.029	2.515GlyTyr: 2.515 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	1.89HisAla: 1.89 ± 0.04	0.366HisCys: 0.366 ± 0.017	1.141HisAsp: 1.141 ± 0.03	1.192HisGlu: 1.192 ± 0.034	1.236HisPhe: 1.236 ± 0.035	1.558HisGly: 1.558 ± 0.04	0.8HisHis: 0.8 ± 0.025	1.447HisIle: 1.447 ± 0.036	1.096HisLys: 1.096 ± 0.025	2.503HisLeu: 2.503 ± 0.046	0.535HisMet: 0.535 ± 0.023	0.911HisAsn: 0.911 ± 0.029	1.299HisPro: 1.299 ± 0.031	1.414HisGln: 1.414 ± 0.038	1.19HisArg: 1.19 ± 0.035	1.527HisSer: 1.527 ± 0.034	1.19HisThr: 1.19 ± 0.029	1.312HisVal: 1.312 ± 0.038	0.416HisTrp: 0.416 ± 0.022	0.947HisTyr: 0.947 ± 0.031	0.001HisXaa: 0.001 ± 0.001
Ile	6.542IleAla: 6.542 ± 0.083	0.652IleCys: 0.652 ± 0.023	3.735IleAsp: 3.735 ± 0.059	4.63IleGlu: 4.63 ± 0.059	2.09IlePhe: 2.09 ± 0.053	4.588IleGly: 4.588 ± 0.066	1.382IleHis: 1.382 ± 0.035	3.182IleIle: 3.182 ± 0.069	2.77IleLys: 2.77 ± 0.057	5.183IleLeu: 5.183 ± 0.075	1.268IleMet: 1.268 ± 0.036	2.61IleAsn: 2.61 ± 0.046	2.582IlePro: 2.582 ± 0.046	2.411IleGln: 2.411 ± 0.04	3.038IleArg: 3.038 ± 0.058	4.063IleSer: 4.063 ± 0.059	3.466IleThr: 3.466 ± 0.055	3.976IleVal: 3.976 ± 0.064	0.615IleTrp: 0.615 ± 0.025	1.661IleTyr: 1.661 ± 0.042	0.001IleXaa: 0.001 ± 0.001
Lys	4.818LysAla: 4.818 ± 0.076	0.29LysCys: 0.29 ± 0.017	2.182LysAsp: 2.182 ± 0.046	3.124LysGlu: 3.124 ± 0.06	1.468LysPhe: 1.468 ± 0.042	3.143LysGly: 3.143 ± 0.063	1.149LysHis: 1.149 ± 0.033	2.791LysIle: 2.791 ± 0.051	2.631LysLys: 2.631 ± 0.056	5.164LysLeu: 5.164 ± 0.07	1.414LysMet: 1.414 ± 0.038	1.96LysAsn: 1.96 ± 0.043	2.42LysPro: 2.42 ± 0.047	2.902LysGln: 2.902 ± 0.051	2.69LysArg: 2.69 ± 0.048	2.83LysSer: 2.83 ± 0.048	2.641LysThr: 2.641 ± 0.053	3.81LysVal: 3.81 ± 0.061	0.539LysTrp: 0.539 ± 0.019	1.225LysTyr: 1.225 ± 0.035	0.0LysXaa: 0.0 ± 0.0
Leu	11.048LeuAla: 11.048 ± 0.112	1.25LeuCys: 1.25 ± 0.038	5.649LeuAsp: 5.649 ± 0.075	6.273LeuGlu: 6.273 ± 0.087	4.389LeuPhe: 4.389 ± 0.073	7.163LeuGly: 7.163 ± 0.088	2.363LeuHis: 2.363 ± 0.047	6.093LeuIle: 6.093 ± 0.078	5.168LeuLys: 5.168 ± 0.074	11.708LeuLeu: 11.708 ± 0.161	2.914LeuMet: 2.914 ± 0.048	4.689LeuAsn: 4.689 ± 0.068	4.976LeuPro: 4.976 ± 0.08	4.312LeuGln: 4.312 ± 0.072	4.986LeuArg: 4.986 ± 0.069	7.98LeuSer: 7.98 ± 0.096	6.288LeuThr: 6.288 ± 0.078	7.34LeuVal: 7.34 ± 0.092	1.228LeuTrp: 1.228 ± 0.041	2.713LeuTyr: 2.713 ± 0.058	0.002LeuXaa: 0.002 ± 0.001
Met	2.761MetAla: 2.761 ± 0.051	0.208MetCys: 0.208 ± 0.014	1.266MetAsp: 1.266 ± 0.035	1.344MetGlu: 1.344 ± 0.032	0.936MetPhe: 0.936 ± 0.031	1.851MetGly: 1.851 ± 0.045	0.544MetHis: 0.544 ± 0.025	1.521MetIle: 1.521 ± 0.036	1.46MetLys: 1.46 ± 0.035	2.969MetLeu: 2.969 ± 0.059	0.892MetMet: 0.892 ± 0.028	1.128MetAsn: 1.128 ± 0.037	1.305MetPro: 1.305 ± 0.038	1.258MetGln: 1.258 ± 0.032	1.384MetArg: 1.384 ± 0.039	2.018MetSer: 2.018 ± 0.039	1.604MetThr: 1.604 ± 0.035	1.989MetVal: 1.989 ± 0.046	0.234MetTrp: 0.234 ± 0.015	0.53MetTyr: 0.53 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	3.37AsnAla: 3.37 ± 0.057	0.375AsnCys: 0.375 ± 0.016	1.99AsnAsp: 1.99 ± 0.049	2.209AsnGlu: 2.209 ± 0.052	1.382AsnPhe: 1.382 ± 0.038	2.759AsnGly: 2.759 ± 0.055	1.027AsnHis: 1.027 ± 0.028	2.377AsnIle: 2.377 ± 0.052	1.894AsnLys: 1.894 ± 0.047	3.977AsnLeu: 3.977 ± 0.064	0.968AsnMet: 0.968 ± 0.03	1.594AsnAsn: 1.594 ± 0.05	2.273AsnPro: 2.273 ± 0.046	2.36AsnGln: 2.36 ± 0.053	2.034AsnArg: 2.034 ± 0.043	2.223AsnSer: 2.223 ± 0.051	1.988AsnThr: 1.988 ± 0.04	2.48AsnVal: 2.48 ± 0.056	0.515AsnTrp: 0.515 ± 0.021	1.185AsnTyr: 1.185 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	3.357ProAla: 3.357 ± 0.062	0.36ProCys: 0.36 ± 0.018	2.182ProAsp: 2.182 ± 0.049	3.186ProGlu: 3.186 ± 0.064	1.762ProPhe: 1.762 ± 0.046	2.121ProGly: 2.121 ± 0.044	0.974ProHis: 0.974 ± 0.033	2.537ProIle: 2.537 ± 0.046	2.03ProLys: 2.03 ± 0.043	4.401ProLeu: 4.401 ± 0.065	1.11ProMet: 1.11 ± 0.031	1.732ProAsn: 1.732 ± 0.042	1.158ProPro: 1.158 ± 0.033	2.026ProGln: 2.026 ± 0.045	1.51ProArg: 1.51 ± 0.042	2.554ProSer: 2.554 ± 0.043	2.26ProThr: 2.26 ± 0.048	3.041ProVal: 3.041 ± 0.057	0.561ProTrp: 0.561 ± 0.023	1.344ProTyr: 1.344 ± 0.034	0.001ProXaa: 0.001 ± 0.001
Gln	5.345GlnAla: 5.345 ± 0.085	0.439GlnCys: 0.439 ± 0.02	2.12GlnAsp: 2.12 ± 0.041	2.841GlnGlu: 2.841 ± 0.05	1.923GlnPhe: 1.923 ± 0.044	3.628GlnGly: 3.628 ± 0.058	1.423GlnHis: 1.423 ± 0.039	2.778GlnIle: 2.778 ± 0.054	2.156GlnLys: 2.156 ± 0.042	5.804GlnLeu: 5.804 ± 0.089	1.292GlnMet: 1.292 ± 0.038	1.673GlnAsn: 1.673 ± 0.04	2.051GlnPro: 2.051 ± 0.043	3.854GlnGln: 3.854 ± 0.096	2.976GlnArg: 2.976 ± 0.055	2.981GlnSer: 2.981 ± 0.061	2.6GlnThr: 2.6 ± 0.046	3.647GlnVal: 3.647 ± 0.058	0.78GlnTrp: 0.78 ± 0.024	1.518GlnTyr: 1.518 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	3.806ArgAla: 3.806 ± 0.066	0.576ArgCys: 0.576 ± 0.023	2.634ArgAsp: 2.634 ± 0.05	3.328ArgGlu: 3.328 ± 0.062	2.473ArgPhe: 2.473 ± 0.044	2.808ArgGly: 2.808 ± 0.054	1.346ArgHis: 1.346 ± 0.037	3.19ArgIle: 3.19 ± 0.056	2.395ArgLys: 2.395 ± 0.054	5.514ArgLeu: 5.514 ± 0.066	1.329ArgMet: 1.329 ± 0.034	1.897ArgAsn: 1.897 ± 0.04	1.72ArgPro: 1.72 ± 0.044	2.546ArgGln: 2.546 ± 0.051	2.674ArgArg: 2.674 ± 0.054	2.821ArgSer: 2.821 ± 0.055	2.269ArgThr: 2.269 ± 0.044	3.373ArgVal: 3.373 ± 0.048	0.719ArgTrp: 0.719 ± 0.026	1.92ArgTyr: 1.92 ± 0.046	0.002ArgXaa: 0.002 ± 0.001
Ser	5.714SerAla: 5.714 ± 0.073	0.562SerCys: 0.562 ± 0.02	3.315SerAsp: 3.315 ± 0.053	3.912SerGlu: 3.912 ± 0.061	2.628SerPhe: 2.628 ± 0.051	4.662SerGly: 4.662 ± 0.079	1.581SerHis: 1.581 ± 0.04	3.867SerIle: 3.867 ± 0.06	2.891SerLys: 2.891 ± 0.048	7.033SerLeu: 7.033 ± 0.08	1.714SerMet: 1.714 ± 0.037	2.279SerAsn: 2.279 ± 0.049	2.462SerPro: 2.462 ± 0.049	3.029SerGln: 3.029 ± 0.054	2.998SerArg: 2.998 ± 0.05	4.21SerSer: 4.21 ± 0.074	3.089SerThr: 3.089 ± 0.051	4.513SerVal: 4.513 ± 0.07	0.813SerTrp: 0.813 ± 0.023	1.917SerTyr: 1.917 ± 0.042	0.001SerXaa: 0.001 ± 0.001
Thr	4.499ThrAla: 4.499 ± 0.065	0.457ThrCys: 0.457 ± 0.022	2.478ThrAsp: 2.478 ± 0.051	3.003ThrGlu: 3.003 ± 0.064	2.015ThrPhe: 2.015 ± 0.039	3.72ThrGly: 3.72 ± 0.069	1.328ThrHis: 1.328 ± 0.037	3.066ThrIle: 3.066 ± 0.055	2.134ThrLys: 2.134 ± 0.049	6.72ThrLeu: 6.72 ± 0.071	1.213ThrMet: 1.213 ± 0.033	1.767ThrAsn: 1.767 ± 0.042	2.691ThrPro: 2.691 ± 0.05	2.942ThrGln: 2.942 ± 0.057	2.352ThrArg: 2.352 ± 0.045	3.061ThrSer: 3.061 ± 0.052	2.735ThrThr: 2.735 ± 0.061	3.71ThrVal: 3.71 ± 0.061	0.576ThrTrp: 0.576 ± 0.024	1.416ThrTyr: 1.416 ± 0.035	0.002ThrXaa: 0.002 ± 0.001
Val	6.81ValAla: 6.81 ± 0.082	0.768ValCys: 0.768 ± 0.026	3.747ValAsp: 3.747 ± 0.065	4.617ValGlu: 4.617 ± 0.063	2.707ValPhe: 2.707 ± 0.053	4.69ValGly: 4.69 ± 0.078	1.318ValHis: 1.318 ± 0.039	4.614ValIle: 4.614 ± 0.07	3.51ValLys: 3.51 ± 0.053	7.21ValLeu: 7.21 ± 0.094	2.098ValMet: 2.098 ± 0.047	2.831ValAsn: 2.831 ± 0.053	2.586ValPro: 2.586 ± 0.049	2.6ValGln: 2.6 ± 0.049	3.238ValArg: 3.238 ± 0.057	4.689ValSer: 4.689 ± 0.063	4.022ValThr: 4.022 ± 0.07	5.641ValVal: 5.641 ± 0.071	0.83ValTrp: 0.83 ± 0.027	1.797ValTyr: 1.797 ± 0.039	0.001ValXaa: 0.001 ± 0.001
Trp	0.879TrpAla: 0.879 ± 0.029	0.169TrpCys: 0.169 ± 0.012	0.612TrpAsp: 0.612 ± 0.028	0.537TrpGlu: 0.537 ± 0.024	0.661TrpPhe: 0.661 ± 0.023	0.774TrpGly: 0.774 ± 0.029	0.387TrpHis: 0.387 ± 0.021	0.72TrpIle: 0.72 ± 0.026	0.504TrpLys: 0.504 ± 0.02	1.946TrpLeu: 1.946 ± 0.045	0.364TrpMet: 0.364 ± 0.018	0.453TrpAsn: 0.453 ± 0.019	0.537TrpPro: 0.537 ± 0.02	1.109TrpGln: 1.109 ± 0.034	0.795TrpArg: 0.795 ± 0.027	0.724TrpSer: 0.724 ± 0.025	0.46TrpThr: 0.46 ± 0.02	0.977TrpVal: 0.977 ± 0.032	0.222TrpTrp: 0.222 ± 0.015	0.351TrpTyr: 0.351 ± 0.017	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.424TyrAla: 2.424 ± 0.053	0.379TyrCys: 0.379 ± 0.016	1.475TyrAsp: 1.475 ± 0.043	1.362TyrGlu: 1.362 ± 0.035	1.35TyrPhe: 1.35 ± 0.031	1.983TyrGly: 1.983 ± 0.047	0.824TyrHis: 0.824 ± 0.028	1.415TyrIle: 1.415 ± 0.035	1.135TyrLys: 1.135 ± 0.036	3.505TyrLeu: 3.505 ± 0.063	0.584TyrMet: 0.584 ± 0.023	0.965TyrAsn: 0.965 ± 0.031	1.408TyrPro: 1.408 ± 0.039	2.217TyrGln: 2.217 ± 0.052	1.916TyrArg: 1.916 ± 0.042	1.91TyrSer: 1.91 ± 0.052	1.448TyrThr: 1.448 ± 0.039	1.84TyrVal: 1.84 ± 0.045	0.52TyrTrp: 0.52 ± 0.019	0.929TyrTyr: 0.929 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.002XaaGlu: 0.002 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 3782 proteins (1153695 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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