Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex; Mycobacterium tuberculosis

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3997 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P9WIF9|PG20_MYCTU Uncharacterized PE-PGRS family protein PE_PGRS20 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=PE_PGRS20 PE=3 SV=1
MM1 pKa = 7.22SFVVAAPEE9 pKa = 4.23VVVAAASDD17 pKa = 3.68LAGIGSAIGAANAAAAVPTMGVLAAGADD45 pKa = 3.78EE46 pKa = 4.59VSAAVADD53 pKa = 4.08LFGAHH58 pKa = 5.81AQAYY62 pKa = 7.57QALSAQAALFHH73 pKa = 6.11EE74 pKa = 4.73QFVHH78 pKa = 7.0AMTAGAGAYY87 pKa = 9.74AGAEE91 pKa = 4.1AADD94 pKa = 3.75AAALDD99 pKa = 4.06VLNGPFQALFGRR111 pKa = 11.84PLIGDD116 pKa = 4.56GANGAPGQPGGPGGLLYY133 pKa = 11.12GNGGNGGNGGIGQPGGAGGDD153 pKa = 3.19AGLIGNGGNGGIGGPGATGLAGGAGGVGGLLFGDD187 pKa = 4.81GGNGGAGGLGTGPVGATGGIGGPGGAAVGLFGHH220 pKa = 6.93GGAGGAGGLGKK231 pKa = 10.5AGFAGGAGGTGGTGGLLYY249 pKa = 11.04GNGGNGGNVPSGAADD264 pKa = 3.84GGAGGDD270 pKa = 3.59ARR272 pKa = 11.84LIGNGGDD279 pKa = 3.64GGSVGAAPTGIGNGGNGGNGGWLYY303 pKa = 11.55GDD305 pKa = 4.35GGSGGSTLQGFSDD318 pKa = 4.35GGTGGNAGMFGDD330 pKa = 4.68GGNGGFSFFDD340 pKa = 3.8GNGGDD345 pKa = 3.87GGTGGTLIGNGGDD358 pKa = 3.67GGNSVQTDD366 pKa = 3.6GFLRR370 pKa = 11.84GHH372 pKa = 6.57GGDD375 pKa = 3.45GGNAVGLIGNGGAGGAGSAGTGVFAPGGGSGGNGGNGALLVGNGGAGGSGGPTQIPSVAVPVTGAGGTGGNGGTAGLIGNGGNGGAAGVSGDD467 pKa = 3.84GTPGTGGNGGYY478 pKa = 10.11AQLIGDD484 pKa = 4.79GGDD487 pKa = 3.6GGPGDD492 pKa = 3.64SGGPGGSGGTGGTLAGQNGSPGGG515 pKa = 3.61

Molecular weight: 43.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P9WHG3|RF1_MYCTU Peptide chain release factor 1 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=prfA PE=1 SV=1
MM1 pKa = 7.46TKK3 pKa = 10.27GKK5 pKa = 8.73RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84SIVSSRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.56GRR42 pKa = 11.84RR43 pKa = 11.84TLSAA47 pKa = 3.76

Molecular weight: 5.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3993

4

3997

1332295

27

4151

333.3

35.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.251 ± 0.067

0.887 ± 0.014

5.811 ± 0.036

4.675 ± 0.044

2.958 ± 0.033

9.982 ± 0.163

2.231 ± 0.018

4.265 ± 0.031

2.036 ± 0.027

9.752 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.966 ± 0.019

2.534 ± 0.065

5.82 ± 0.042

3.091 ± 0.023

7.312 ± 0.059

5.49 ± 0.031

5.925 ± 0.033

8.476 ± 0.045

1.465 ± 0.015

2.074 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski