Amino acid dipepetide frequency for Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.195AlaAla: 21.195 ± 0.242	1.07AlaCys: 1.07 ± 0.025	7.873AlaAsp: 7.873 ± 0.077	7.09AlaGlu: 7.09 ± 0.099	3.332AlaPhe: 3.332 ± 0.057	14.154AlaGly: 14.154 ± 0.311	2.674AlaHis: 2.674 ± 0.048	5.394AlaIle: 5.394 ± 0.063	2.922AlaLys: 2.922 ± 0.055	12.62AlaLeu: 12.62 ± 0.122	2.857AlaMet: 2.857 ± 0.044	3.116AlaAsn: 3.116 ± 0.081	6.176AlaPro: 6.176 ± 0.092	4.262AlaGln: 4.262 ± 0.066	8.761AlaArg: 8.761 ± 0.098	6.312AlaSer: 6.312 ± 0.075	7.384AlaThr: 7.384 ± 0.074	11.351AlaVal: 11.351 ± 0.117	1.645AlaTrp: 1.645 ± 0.037	2.319AlaTyr: 2.319 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	1.161CysAla: 1.161 ± 0.033	0.131CysCys: 0.131 ± 0.01	0.599CysAsp: 0.599 ± 0.023	0.425CysGlu: 0.425 ± 0.019	0.226CysPhe: 0.226 ± 0.011	1.031CysGly: 1.031 ± 0.029	0.223CysHis: 0.223 ± 0.014	0.261CysIle: 0.261 ± 0.014	0.146CysLys: 0.146 ± 0.01	0.647CysLeu: 0.647 ± 0.024	0.155CysMet: 0.155 ± 0.011	0.184CysAsn: 0.184 ± 0.012	0.567CysPro: 0.567 ± 0.02	0.257CysGln: 0.257 ± 0.014	0.758CysArg: 0.758 ± 0.027	0.551CysSer: 0.551 ± 0.019	0.479CysThr: 0.479 ± 0.021	0.696CysVal: 0.696 ± 0.024	0.158CysTrp: 0.158 ± 0.012	0.215CysTyr: 0.215 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	7.111AspAla: 7.111 ± 0.077	0.48AspCys: 0.48 ± 0.021	3.629AspAsp: 3.629 ± 0.061	3.534AspGlu: 3.534 ± 0.065	1.663AspPhe: 1.663 ± 0.036	5.815AspGly: 5.815 ± 0.101	1.383AspHis: 1.383 ± 0.033	2.548AspIle: 2.548 ± 0.048	1.201AspLys: 1.201 ± 0.034	5.62AspLeu: 5.62 ± 0.075	0.908AspMet: 0.908 ± 0.027	1.211AspAsn: 1.211 ± 0.032	4.117AspPro: 4.117 ± 0.055	1.747AspGln: 1.747 ± 0.042	4.338AspArg: 4.338 ± 0.06	2.628AspSer: 2.628 ± 0.05	2.951AspThr: 2.951 ± 0.054	4.972AspVal: 4.972 ± 0.081	0.88AspTrp: 0.88 ± 0.026	1.377AspTyr: 1.377 ± 0.031	0.0AspXaa: 0.0 ± 0.0
Glu	5.53GluAla: 5.53 ± 0.075	0.402GluCys: 0.402 ± 0.02	1.94GluAsp: 1.94 ± 0.044	2.081GluGlu: 2.081 ± 0.047	1.689GluPhe: 1.689 ± 0.036	2.895GluGly: 2.895 ± 0.056	1.479GluHis: 1.479 ± 0.037	2.415GluIle: 2.415 ± 0.047	1.079GluLys: 1.079 ± 0.031	6.298GluLeu: 6.298 ± 0.091	0.946GluMet: 0.946 ± 0.031	0.953GluAsn: 0.953 ± 0.025	2.735GluPro: 2.735 ± 0.053	2.033GluGln: 2.033 ± 0.05	3.964GluArg: 3.964 ± 0.069	2.444GluSer: 2.444 ± 0.049	2.259GluThr: 2.259 ± 0.046	4.002GluVal: 4.002 ± 0.062	0.604GluTrp: 0.604 ± 0.021	1.0GluTyr: 1.0 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	3.903PheAla: 3.903 ± 0.054	0.311PheCys: 0.311 ± 0.016	2.248PheAsp: 2.248 ± 0.039	1.423PheGlu: 1.423 ± 0.035	1.007PhePhe: 1.007 ± 0.038	3.802PheGly: 3.802 ± 0.085	0.616PheHis: 0.616 ± 0.019	1.04PheIle: 1.04 ± 0.026	0.473PheLys: 0.473 ± 0.02	2.333PheLeu: 2.333 ± 0.048	0.446PheMet: 0.446 ± 0.021	1.037PheAsn: 1.037 ± 0.101	1.404PhePro: 1.404 ± 0.044	0.679PheGln: 0.679 ± 0.021	1.654PheArg: 1.654 ± 0.033	1.679PheSer: 1.679 ± 0.04	1.927PheThr: 1.927 ± 0.036	2.532PheVal: 2.532 ± 0.047	0.41PheTrp: 0.41 ± 0.019	0.654PheTyr: 0.654 ± 0.027	0.0PheXaa: 0.0 ± 0.0
Gly	12.036GlyAla: 12.036 ± 0.301	0.906GlyCys: 0.906 ± 0.031	5.325GlyAsp: 5.325 ± 0.092	4.034GlyGlu: 4.034 ± 0.064	3.369GlyPhe: 3.369 ± 0.123	12.534GlyGly: 12.534 ± 0.728	2.115GlyHis: 2.115 ± 0.042	4.331GlyIle: 4.331 ± 0.08	2.334GlyLys: 2.334 ± 0.055	9.147GlyLeu: 9.147 ± 0.123	2.173GlyMet: 2.173 ± 0.04	3.732GlyAsn: 3.732 ± 0.226	4.751GlyPro: 4.751 ± 0.065	3.075GlyGln: 3.075 ± 0.057	6.258GlyArg: 6.258 ± 0.074	6.16GlySer: 6.16 ± 0.108	5.678GlyThr: 5.678 ± 0.143	7.801GlyVal: 7.801 ± 0.096	1.714GlyTrp: 1.714 ± 0.04	2.341GlyTyr: 2.341 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	2.569HisAla: 2.569 ± 0.052	0.215HisCys: 0.215 ± 0.012	1.356HisAsp: 1.356 ± 0.033	1.018HisGlu: 1.018 ± 0.032	0.636HisPhe: 0.636 ± 0.019	2.212HisGly: 2.212 ± 0.044	0.712HisHis: 0.712 ± 0.028	0.862HisIle: 0.862 ± 0.024	0.417HisLys: 0.417 ± 0.017	2.069HisLeu: 2.069 ± 0.034	0.338HisMet: 0.338 ± 0.015	0.535HisAsn: 0.535 ± 0.019	1.689HisPro: 1.689 ± 0.042	0.703HisGln: 0.703 ± 0.024	2.012HisArg: 2.012 ± 0.041	1.102HisSer: 1.102 ± 0.025	1.287HisThr: 1.287 ± 0.031	1.678HisVal: 1.678 ± 0.037	0.373HisTrp: 0.373 ± 0.017	0.529HisTyr: 0.529 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	6.282IleAla: 6.282 ± 0.085	0.42IleCys: 0.42 ± 0.017	3.327IleAsp: 3.327 ± 0.053	2.449IleGlu: 2.449 ± 0.054	1.02IlePhe: 1.02 ± 0.03	5.037IleGly: 5.037 ± 0.117	0.796IleHis: 0.796 ± 0.028	1.386IleIle: 1.386 ± 0.038	0.877IleLys: 0.877 ± 0.032	2.913IleLeu: 2.913 ± 0.048	0.621IleMet: 0.621 ± 0.021	1.272IleAsn: 1.272 ± 0.032	2.559IlePro: 2.559 ± 0.077	0.938IleGln: 0.938 ± 0.03	2.649IleArg: 2.649 ± 0.046	2.391IleSer: 2.391 ± 0.048	2.812IleThr: 2.812 ± 0.052	3.611IleVal: 3.611 ± 0.059	0.487IleTrp: 0.487 ± 0.019	0.802IleTyr: 0.802 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	2.462LysAla: 2.462 ± 0.054	0.118LysCys: 0.118 ± 0.01	1.015LysAsp: 1.015 ± 0.027	0.835LysGlu: 0.835 ± 0.029	0.545LysPhe: 0.545 ± 0.02	1.493LysGly: 1.493 ± 0.043	0.476LysHis: 0.476 ± 0.018	0.957LysIle: 0.957 ± 0.027	0.571LysLys: 0.571 ± 0.024	2.193LysLeu: 2.193 ± 0.047	0.428LysMet: 0.428 ± 0.021	0.477LysAsn: 0.477 ± 0.019	1.42LysPro: 1.42 ± 0.043	0.679LysGln: 0.679 ± 0.023	1.59LysArg: 1.59 ± 0.038	1.205LysSer: 1.205 ± 0.033	1.347LysThr: 1.347 ± 0.033	1.819LysVal: 1.819 ± 0.047	0.292LysTrp: 0.292 ± 0.014	0.438LysTyr: 0.438 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	14.379LeuAla: 14.379 ± 0.137	0.887LeuCys: 0.887 ± 0.025	6.056LeuAsp: 6.056 ± 0.084	3.827LeuGlu: 3.827 ± 0.058	2.752LeuPhe: 2.752 ± 0.063	8.795LeuGly: 8.795 ± 0.107	2.067LeuHis: 2.067 ± 0.042	4.1LeuIle: 4.1 ± 0.061	1.699LeuLys: 1.699 ± 0.036	9.676LeuLeu: 9.676 ± 0.118	1.729LeuMet: 1.729 ± 0.041	2.188LeuAsn: 2.188 ± 0.051	5.945LeuPro: 5.945 ± 0.08	2.682LeuGln: 2.682 ± 0.047	7.768LeuArg: 7.768 ± 0.095	5.572LeuSer: 5.572 ± 0.067	6.186LeuThr: 6.186 ± 0.076	8.345LeuVal: 8.345 ± 0.101	1.234LeuTrp: 1.234 ± 0.035	1.636LeuTyr: 1.636 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	2.584MetAla: 2.584 ± 0.045	0.197MetCys: 0.197 ± 0.011	0.773MetAsp: 0.773 ± 0.024	0.608MetGlu: 0.608 ± 0.024	0.626MetPhe: 0.626 ± 0.022	1.48MetGly: 1.48 ± 0.033	0.423MetHis: 0.423 ± 0.019	0.85MetIle: 0.85 ± 0.028	0.411MetLys: 0.411 ± 0.017	2.069MetLeu: 2.069 ± 0.041	0.403MetMet: 0.403 ± 0.021	0.489MetAsn: 0.489 ± 0.019	1.215MetPro: 1.215 ± 0.032	0.534MetGln: 0.534 ± 0.022	1.505MetArg: 1.505 ± 0.038	1.539MetSer: 1.539 ± 0.031	1.676MetThr: 1.676 ± 0.034	1.664MetVal: 1.664 ± 0.037	0.283MetTrp: 0.283 ± 0.014	0.329MetTyr: 0.329 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.095AsnAla: 3.095 ± 0.066	0.189AsnCys: 0.189 ± 0.014	1.187AsnAsp: 1.187 ± 0.031	0.928AsnGlu: 0.928 ± 0.028	0.808AsnPhe: 0.808 ± 0.038	3.157AsnGly: 3.157 ± 0.161	0.478AsnHis: 0.478 ± 0.018	1.227AsnIle: 1.227 ± 0.091	0.427AsnLys: 0.427 ± 0.018	2.334AsnLeu: 2.334 ± 0.074	0.42AsnMet: 0.42 ± 0.018	0.724AsnAsn: 0.724 ± 0.042	1.822AsnPro: 1.822 ± 0.04	0.744AsnGln: 0.744 ± 0.024	1.633AsnArg: 1.633 ± 0.039	1.503AsnSer: 1.503 ± 0.08	1.706AsnThr: 1.706 ± 0.123	1.973AsnVal: 1.973 ± 0.076	0.381AsnTrp: 0.381 ± 0.019	0.603AsnTyr: 0.603 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	7.489ProAla: 7.489 ± 0.108	0.366ProCys: 0.366 ± 0.019	4.012ProAsp: 4.012 ± 0.055	3.203ProGlu: 3.203 ± 0.054	1.581ProPhe: 1.581 ± 0.038	6.098ProGly: 6.098 ± 0.088	1.205ProHis: 1.205 ± 0.031	2.304ProIle: 2.304 ± 0.055	1.261ProLys: 1.261 ± 0.038	4.943ProLeu: 4.943 ± 0.061	1.196ProMet: 1.196 ± 0.029	1.365ProAsn: 1.365 ± 0.038	4.046ProPro: 4.046 ± 0.107	1.903ProGln: 1.903 ± 0.041	3.554ProArg: 3.554 ± 0.061	3.148ProSer: 3.148 ± 0.063	3.563ProThr: 3.563 ± 0.058	4.97ProVal: 4.97 ± 0.072	0.853ProTrp: 0.853 ± 0.024	1.138ProTyr: 1.138 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	4.009GlnAla: 4.009 ± 0.064	0.24GlnCys: 0.24 ± 0.013	1.195GlnAsp: 1.195 ± 0.032	1.043GlnGlu: 1.043 ± 0.029	0.955GlnPhe: 0.955 ± 0.03	2.102GlnGly: 2.102 ± 0.05	0.787GlnHis: 0.787 ± 0.025	1.536GlnIle: 1.536 ± 0.035	0.549GlnLys: 0.549 ± 0.023	3.804GlnLeu: 3.804 ± 0.066	0.672GlnMet: 0.672 ± 0.024	0.646GlnAsn: 0.646 ± 0.025	2.039GlnPro: 2.039 ± 0.048	1.427GlnGln: 1.427 ± 0.04	2.981GlnArg: 2.981 ± 0.055	1.445GlnSer: 1.445 ± 0.032	1.727GlnThr: 1.727 ± 0.038	2.651GlnVal: 2.651 ± 0.047	0.498GlnTrp: 0.498 ± 0.019	0.608GlnTyr: 0.608 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	8.452ArgAla: 8.452 ± 0.103	0.801ArgCys: 0.801 ± 0.028	3.992ArgAsp: 3.992 ± 0.063	3.465ArgGlu: 3.465 ± 0.052	2.421ArgPhe: 2.421 ± 0.044	5.316ArgGly: 5.316 ± 0.072	1.899ArgHis: 1.899 ± 0.04	3.445ArgIle: 3.445 ± 0.049	1.582ArgLys: 1.582 ± 0.036	7.695ArgLeu: 7.695 ± 0.084	1.785ArgMet: 1.785 ± 0.034	1.67ArgAsn: 1.67 ± 0.039	4.166ArgPro: 4.166 ± 0.063	2.43ArgGln: 2.43 ± 0.043	7.15ArgArg: 7.15 ± 0.101	4.064ArgSer: 4.064 ± 0.063	3.816ArgThr: 3.816 ± 0.057	5.612ArgVal: 5.612 ± 0.082	1.431ArgTrp: 1.431 ± 0.037	1.931ArgTyr: 1.931 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	7.325SerAla: 7.325 ± 0.081	0.453SerCys: 0.453 ± 0.022	2.954SerAsp: 2.954 ± 0.041	2.327SerGlu: 2.327 ± 0.042	1.585SerPhe: 1.585 ± 0.041	6.563SerGly: 6.563 ± 0.152	1.097SerHis: 1.097 ± 0.028	2.244SerIle: 2.244 ± 0.043	1.072SerLys: 1.072 ± 0.03	4.807SerLeu: 4.807 ± 0.068	1.28SerMet: 1.28 ± 0.033	1.245SerAsn: 1.245 ± 0.035	3.182SerPro: 3.182 ± 0.062	1.595SerGln: 1.595 ± 0.037	3.835SerArg: 3.835 ± 0.055	3.185SerSer: 3.185 ± 0.055	3.353SerThr: 3.353 ± 0.055	4.514SerVal: 4.514 ± 0.064	0.961SerTrp: 0.961 ± 0.029	1.32SerTyr: 1.32 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	7.653ThrAla: 7.653 ± 0.083	0.459ThrCys: 0.459 ± 0.02	3.321ThrAsp: 3.321 ± 0.052	2.815ThrGlu: 2.815 ± 0.055	1.663ThrPhe: 1.663 ± 0.04	6.825ThrGly: 6.825 ± 0.193	1.2ThrHis: 1.2 ± 0.03	2.573ThrIle: 2.573 ± 0.054	1.192ThrLys: 1.192 ± 0.031	5.462ThrLeu: 5.462 ± 0.062	1.243ThrMet: 1.243 ± 0.03	1.411ThrAsn: 1.411 ± 0.04	3.783ThrPro: 3.783 ± 0.069	1.593ThrGln: 1.593 ± 0.036	3.553ThrArg: 3.553 ± 0.053	3.139ThrSer: 3.139 ± 0.052	3.903ThrThr: 3.903 ± 0.064	5.515ThrVal: 5.515 ± 0.069	0.762ThrTrp: 0.762 ± 0.029	1.179ThrTyr: 1.179 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	11.373ValAla: 11.373 ± 0.093	0.82ValCys: 0.82 ± 0.025	5.331ValAsp: 5.331 ± 0.077	4.091ValGlu: 4.091 ± 0.071	2.463ValPhe: 2.463 ± 0.042	7.531ValGly: 7.531 ± 0.105	1.813ValHis: 1.813 ± 0.04	3.953ValIle: 3.953 ± 0.06	1.572ValLys: 1.572 ± 0.043	8.68ValLeu: 8.68 ± 0.088	1.492ValMet: 1.492 ± 0.034	2.128ValAsn: 2.128 ± 0.047	4.476ValPro: 4.476 ± 0.06	2.154ValGln: 2.154 ± 0.036	5.912ValArg: 5.912 ± 0.077	4.805ValSer: 4.805 ± 0.061	5.286ValThr: 5.286 ± 0.072	8.362ValVal: 8.362 ± 0.101	1.003ValTrp: 1.003 ± 0.026	1.512ValTyr: 1.512 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	1.519TrpAla: 1.519 ± 0.038	0.173TrpCys: 0.173 ± 0.012	0.76TrpAsp: 0.76 ± 0.027	0.591TrpGlu: 0.591 ± 0.021	0.507TrpPhe: 0.507 ± 0.018	0.963TrpGly: 0.963 ± 0.03	0.35TrpHis: 0.35 ± 0.016	0.6TrpIle: 0.6 ± 0.02	0.266TrpLys: 0.266 ± 0.013	1.873TrpLeu: 1.873 ± 0.04	0.313TrpMet: 0.313 ± 0.015	0.398TrpAsn: 0.398 ± 0.02	0.853TrpPro: 0.853 ± 0.029	0.652TrpGln: 0.652 ± 0.022	1.342TrpArg: 1.342 ± 0.039	0.928TrpSer: 0.928 ± 0.03	0.795TrpThr: 0.795 ± 0.025	1.089TrpVal: 1.089 ± 0.031	0.343TrpTrp: 0.343 ± 0.018	0.339TrpTyr: 0.339 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.379TyrAla: 2.379 ± 0.04	0.231TyrCys: 0.231 ± 0.013	1.22TyrAsp: 1.22 ± 0.034	0.961TyrGlu: 0.961 ± 0.03	0.728TyrPhe: 0.728 ± 0.026	2.013TyrGly: 2.013 ± 0.04	0.516TyrHis: 0.516 ± 0.019	0.621TyrIle: 0.621 ± 0.023	0.31TyrLys: 0.31 ± 0.014	2.34TyrLeu: 2.34 ± 0.047	0.254TyrMet: 0.254 ± 0.014	0.556TyrAsn: 0.556 ± 0.047	1.254TyrPro: 1.254 ± 0.037	0.826TyrGln: 0.826 ± 0.026	1.907TyrArg: 1.907 ± 0.042	1.1TyrSer: 1.1 ± 0.029	1.111TyrThr: 1.111 ± 0.031	1.599TyrVal: 1.599 ± 0.035	0.342TyrTrp: 0.342 ± 0.016	0.468TyrTyr: 0.468 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3997 proteins (1332295 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---