Sphingobacterium paucimobilis HER1398
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4220 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U2HWK2|U2HWK2_9SPHI Endo/exonuclease/phosphatase domain-containing protein OS=Sphingobacterium paucimobilis HER1398 OX=1346330 GN=M472_14225 PE=4 SV=1
MM1 pKa = 8.07 IYY3 pKa = 10.15 IFATSVEE10 pKa = 4.32 SDD12 pKa = 3.2 QDD14 pKa = 3.47 IQTLKK19 pKa = 10.63 PYY21 pKa = 10.76 LEE23 pKa = 4.36 EE24 pKa = 3.93 QLPDD28 pKa = 3.42 ARR30 pKa = 11.84 WNFDD34 pKa = 5.7 LEE36 pKa = 4.42 DD37 pKa = 4.43 CDD39 pKa = 4.75 NIFRR43 pKa = 11.84 VDD45 pKa = 3.57 TPTSKK50 pKa = 10.69 AAAIIEE56 pKa = 4.13 LLVEE60 pKa = 4.2 LGYY63 pKa = 10.95 NCEE66 pKa = 4.07 EE67 pKa = 4.21 LPYY70 pKa = 11.09
Molecular weight: 8.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.761
IPC2_protein 3.795
IPC_protein 3.706
Toseland 3.516
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.617
Rodwell 3.541
Grimsley 3.439
Solomon 3.656
Lehninger 3.617
Nozaki 3.821
DTASelect 3.973
Thurlkill 3.579
EMBOSS 3.63
Sillero 3.821
Patrickios 0.693
IPC_peptide 3.656
IPC2_peptide 3.795
IPC2.peptide.svr19 3.751
Protein with the highest isoelectric point:
>tr|U2HUD9|U2HUD9_9SPHI Uncharacterized protein OS=Sphingobacterium paucimobilis HER1398 OX=1346330 GN=M472_10090 PE=4 SV=1
MM1 pKa = 7.86 CKK3 pKa = 9.52 PDD5 pKa = 3.51 LKK7 pKa = 10.77 RR8 pKa = 11.84 YY9 pKa = 8.09 MFLKK13 pKa = 10.55 ALRR16 pKa = 11.84 NSWQQYY22 pKa = 10.85 NILCLLDD29 pKa = 4.23 RR30 pKa = 11.84 FLLFPTTVPTVSGSKK45 pKa = 10.45 GLSQSITMDD54 pKa = 3.39 TPKK57 pKa = 10.75 ARR59 pKa = 11.84 VKK61 pKa = 10.07 IAFFRR66 pKa = 11.84 RR67 pKa = 11.84 SRR69 pKa = 11.84 YY70 pKa = 9.68 LSLSYY75 pKa = 9.95 WSQFAGGITAEE86 pKa = 3.86 ITGNRR91 pKa = 11.84 EE92 pKa = 3.51 GRR94 pKa = 11.84 SS95 pKa = 3.4
Molecular weight: 10.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.765
IPC_protein 10.379
Toseland 10.511
ProMoST 10.175
Dawson 10.643
Bjellqvist 10.35
Wikipedia 10.833
Rodwell 10.979
Grimsley 10.716
Solomon 10.716
Lehninger 10.687
Nozaki 10.496
DTASelect 10.335
Thurlkill 10.526
EMBOSS 10.906
Sillero 10.57
Patrickios 10.716
IPC_peptide 10.716
IPC2_peptide 9.355
IPC2.peptide.svr19 8.499
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4220
0
4220
1481620
31
4977
351.1
39.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.983 ± 0.04
0.691 ± 0.013
5.636 ± 0.025
6.145 ± 0.033
4.782 ± 0.031
6.75 ± 0.04
1.854 ± 0.019
7.161 ± 0.032
6.772 ± 0.035
9.621 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.296 ± 0.018
5.435 ± 0.035
3.528 ± 0.018
3.846 ± 0.023
4.309 ± 0.029
6.619 ± 0.027
5.58 ± 0.039
6.503 ± 0.036
1.153 ± 0.013
4.337 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here