Struthio camelus australis
Average proteome isoelectric point is 7.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13815 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A093I8R0|A0A093I8R0_STRCA Uncharacterized protein (Fragment) OS=Struthio camelus australis OX=441894 GN=N308_14746 PE=4 SV=1
SS1 pKa = 6.83 RR2 pKa = 11.84 LSWLDD7 pKa = 3.18 NSGLGFSLDD16 pKa = 3.61 YY17 pKa = 9.22 PTISLHH23 pKa = 6.36 AVSRR27 pKa = 11.84 DD28 pKa = 3.07 LNAYY32 pKa = 7.9 PWEE35 pKa = 4.19 HH36 pKa = 6.86 LYY38 pKa = 11.74 VMVNAKK44 pKa = 10.34 FEE46 pKa = 4.16 EE47 pKa = 4.43 EE48 pKa = 4.19 DD49 pKa = 3.44 AKK51 pKa = 10.22 EE52 pKa = 3.98 APMAEE57 pKa = 4.31 GEE59 pKa = 4.22 EE60 pKa = 4.57 EE61 pKa = 4.26 EE62 pKa = 6.12 DD63 pKa = 3.7 SDD65 pKa = 6.27 DD66 pKa = 4.98 DD67 pKa = 3.91 IEE69 pKa = 5.28 PIAEE73 pKa = 4.0 FRR75 pKa = 11.84 FVPSDD80 pKa = 3.36 KK81 pKa = 10.88 SALEE85 pKa = 3.9 AMFSAMCEE93 pKa = 4.27 CQALHH98 pKa = 7.39 PDD100 pKa = 4.25 PEE102 pKa = 6.28 DD103 pKa = 3.73 EE104 pKa = 6.39 DD105 pKa = 4.23 SDD107 pKa = 3.72 NDD109 pKa = 4.0 YY110 pKa = 11.37 EE111 pKa = 4.59 GEE113 pKa = 4.16 EE114 pKa = 4.41 YY115 pKa = 10.56 DD116 pKa = 4.87 VEE118 pKa = 4.22 AHH120 pKa = 6.63 EE121 pKa = 5.44 LGQGDD126 pKa = 3.65 IPSFYY131 pKa = 9.93 TYY133 pKa = 11.03 EE134 pKa = 4.17 EE135 pKa = 4.58 GLSHH139 pKa = 6.9 LTAEE143 pKa = 4.71 GQATLEE149 pKa = 4.11 RR150 pKa = 11.84 LEE152 pKa = 4.74 GMLAQSVSSQYY163 pKa = 11.66 NMAGVRR169 pKa = 11.84 TEE171 pKa = 3.82 DD172 pKa = 3.14 SVRR175 pKa = 11.84 EE176 pKa = 4.08 FEE178 pKa = 6.01 DD179 pKa = 3.69 GMEE182 pKa = 3.99 VDD184 pKa = 3.99 IAPAVAGQFEE194 pKa = 4.53 DD195 pKa = 5.38 AEE197 pKa = 4.32 VDD199 pKa = 3.59 HH200 pKa = 7.08
Molecular weight: 22.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.783
IPC_protein 3.757
Toseland 3.567
ProMoST 3.884
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.617
Rodwell 3.592
Grimsley 3.478
Solomon 3.719
Lehninger 3.681
Nozaki 3.834
DTASelect 3.999
Thurlkill 3.605
EMBOSS 3.63
Sillero 3.872
Patrickios 1.837
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.793
Protein with the highest isoelectric point:
>tr|A0A093JTT5|A0A093JTT5_STRCA Multiple coagulation factor deficiency protein 2 OS=Struthio camelus australis OX=441894 GN=N308_10602 PE=4 SV=1
PP1 pKa = 7.17 TPRR4 pKa = 11.84 PMPAPTPAPRR14 pKa = 11.84 SAPTPVPISAPTPRR28 pKa = 11.84 SAPTPAPRR36 pKa = 11.84 SAPTPVPISAPTPRR50 pKa = 11.84 SAPTPAPRR58 pKa = 11.84 SAPTPVPISAPTPRR72 pKa = 11.84 SAPTPAPRR80 pKa = 11.84 SAPTPVPISAPIPAPTPAPAPRR102 pKa = 11.84 ATAISAPTPAPILRR116 pKa = 11.84 SAPTPP121 pKa = 3.61
Molecular weight: 11.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.513
IPC2_protein 11.184
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.457
Grimsley 12.998
Solomon 13.451
Lehninger 13.349
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.193
IPC_peptide 13.451
IPC2_peptide 12.442
IPC2.peptide.svr19 9.197
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13815
0
13815
5684286
31
5575
411.5
46.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.242 ± 0.019
2.195 ± 0.018
4.996 ± 0.016
7.231 ± 0.026
3.962 ± 0.016
5.731 ± 0.023
2.603 ± 0.01
5.041 ± 0.017
6.542 ± 0.026
9.859 ± 0.028
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.244 ± 0.01
4.301 ± 0.014
5.172 ± 0.027
4.619 ± 0.019
5.286 ± 0.017
8.139 ± 0.034
5.362 ± 0.015
6.324 ± 0.016
1.193 ± 0.008
2.954 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here