Thalassobacillus cyri

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Thalassobacillus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4309 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H3Y562|A0A1H3Y562_9BACI MFS transporter DHA2 family metal-tetracycline-proton antiporter OS=Thalassobacillus cyri OX=571932 GN=SAMN05421743_102348 PE=4 SV=1
MM1 pKa = 7.84DD2 pKa = 5.83LSVNDD7 pKa = 4.52FNACEE12 pKa = 4.56LPFCAQTNDD21 pKa = 3.64TAPSFQAEE29 pKa = 4.49AYY31 pKa = 10.09DD32 pKa = 3.7NTTKK36 pKa = 10.73KK37 pKa = 10.27IIPINLEE44 pKa = 3.7NYY46 pKa = 7.28QGKK49 pKa = 7.66WVVLFFYY56 pKa = 10.76ASDD59 pKa = 3.63FTFVV63 pKa = 3.12

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H3X9S2|A0A1H3X9S2_9BACI Uncharacterized membrane protein YkvI OS=Thalassobacillus cyri OX=571932 GN=SAMN05421743_10290 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.12KK14 pKa = 8.46VHH16 pKa = 5.5GFRR19 pKa = 11.84TRR21 pKa = 11.84MSTKK25 pKa = 10.06NGRR28 pKa = 11.84KK29 pKa = 8.49VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4309

0

4309

1218288

27

1965

282.7

31.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.31 ± 0.038

0.623 ± 0.011

5.401 ± 0.033

7.881 ± 0.053

4.392 ± 0.033

7.256 ± 0.038

2.207 ± 0.02

7.292 ± 0.041

6.511 ± 0.037

9.451 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.983 ± 0.02

4.165 ± 0.024

3.791 ± 0.025

3.87 ± 0.029

4.091 ± 0.026

5.708 ± 0.022

5.393 ± 0.025

7.074 ± 0.029

1.097 ± 0.015

3.506 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski