Bacteriovorax stolpii
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3721 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K9NV78|A0A2K9NV78_9PROT Uncharacterized protein OS=Bacteriovorax stolpii OX=960 GN=C0V70_15150 PE=3 SV=1
MM1 pKa = 7.39 SNQVRR6 pKa = 11.84 VIDD9 pKa = 4.17 SLSGTCLFEE18 pKa = 3.98 TTIDD22 pKa = 4.32 KK23 pKa = 11.16 INDD26 pKa = 3.12 AYY28 pKa = 10.94 AFATQMEE35 pKa = 4.63 EE36 pKa = 3.62 AGLDD40 pKa = 3.57 IEE42 pKa = 5.03 VVAPGLAEE50 pKa = 3.82 TLIRR54 pKa = 11.84 SLGADD59 pKa = 3.33 DD60 pKa = 4.83 TEE62 pKa = 4.19 IKK64 pKa = 10.31 AYY66 pKa = 9.03 QQSLQDD72 pKa = 4.47 EE73 pKa = 4.53 IDD75 pKa = 3.31 EE76 pKa = 5.4 HH77 pKa = 6.83 EE78 pKa = 4.77 DD79 pKa = 3.09 SDD81 pKa = 4.6 YY82 pKa = 11.78 GCAICPPGPHH92 pKa = 6.98 KK93 pKa = 10.92
Molecular weight: 10.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.767
IPC2_protein 3.935
IPC_protein 3.872
Toseland 3.681
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.592
Solomon 3.846
Lehninger 3.795
Nozaki 3.986
DTASelect 4.177
Thurlkill 3.732
EMBOSS 3.795
Sillero 3.999
Patrickios 1.901
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.886
Protein with the highest isoelectric point:
>tr|A0A2K9NXB6|A0A2K9NXB6_9PROT Transcriptional regulator OS=Bacteriovorax stolpii OX=960 GN=C0V70_04535 PE=4 SV=1
MM1 pKa = 7.83 SKK3 pKa = 9.06 RR4 pKa = 11.84 TWQPKK9 pKa = 7.8 KK10 pKa = 10.19 LKK12 pKa = 9.74 RR13 pKa = 11.84 LRR15 pKa = 11.84 VHH17 pKa = 6.85 GFLKK21 pKa = 10.64 RR22 pKa = 11.84 MASPGGRR29 pKa = 11.84 SVLNARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.04 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LTVSSSTKK50 pKa = 10.39
Molecular weight: 5.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.508
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.676
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.398
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.125
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3721
0
3721
1179481
27
2950
317.0
35.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.996 ± 0.033
0.983 ± 0.016
5.271 ± 0.027
6.788 ± 0.041
5.201 ± 0.035
6.285 ± 0.035
2.0 ± 0.021
6.865 ± 0.035
8.146 ± 0.037
9.881 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.638 ± 0.019
4.948 ± 0.029
3.624 ± 0.024
3.233 ± 0.023
3.898 ± 0.026
6.978 ± 0.036
5.408 ± 0.035
6.401 ± 0.034
0.952 ± 0.013
3.505 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here