Salinigranum rubrum
Average proteome isoelectric point is 5.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4473 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I8VKG5|A0A2I8VKG5_9EURY HGPRTase-like protein OS=Salinigranum rubrum OX=755307 GN=C2R22_12830 PE=3 SV=1
MM1 pKa = 7.59 AIDD4 pKa = 5.32 ANFEE8 pKa = 4.32 TNRR11 pKa = 11.84 EE12 pKa = 3.91 RR13 pKa = 11.84 VEE15 pKa = 3.93 VDD17 pKa = 3.22 DD18 pKa = 6.31 DD19 pKa = 4.28 AFADD23 pKa = 4.87 DD24 pKa = 3.78 VAVWGPYY31 pKa = 9.85 DD32 pKa = 3.77 PPEE35 pKa = 4.07 KK36 pKa = 10.31 QGIHH40 pKa = 5.21 GTHH43 pKa = 5.77 VAVDD47 pKa = 3.98 FDD49 pKa = 3.53 ICLADD54 pKa = 4.17 GACLEE59 pKa = 4.7 DD60 pKa = 4.34 CPVDD64 pKa = 3.46 VFTWVDD70 pKa = 3.61 TPGHH74 pKa = 6.6 PEE76 pKa = 3.91 SEE78 pKa = 4.13 IKK80 pKa = 10.77 AEE82 pKa = 4.03 PTHH85 pKa = 6.7 EE86 pKa = 4.32 DD87 pKa = 3.1 QCIDD91 pKa = 3.86 CMLCVDD97 pKa = 4.72 VCPVDD102 pKa = 5.73 AIDD105 pKa = 4.18 VDD107 pKa = 3.82 PGRR110 pKa = 11.84 AGRR113 pKa = 11.84 II114 pKa = 3.62
Molecular weight: 12.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.77
IPC_protein 3.77
Toseland 3.541
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.732
Rodwell 3.592
Grimsley 3.452
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.151
Thurlkill 3.605
EMBOSS 3.732
Sillero 3.897
Patrickios 1.036
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.792
Protein with the highest isoelectric point:
>tr|A0A2I8VPT2|A0A2I8VPT2_9EURY Translation initiation factor IF-2 subunit beta OS=Salinigranum rubrum OX=755307 GN=C2R22_16970 PE=3 SV=1
MM1 pKa = 7.43 RR2 pKa = 11.84 RR3 pKa = 11.84 SRR5 pKa = 11.84 LRR7 pKa = 11.84 PGPVSSTATPRR18 pKa = 11.84 RR19 pKa = 11.84 LRR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 SLFRR27 pKa = 11.84 FRR29 pKa = 11.84 LLPRR33 pKa = 11.84 RR34 pKa = 11.84 LRR36 pKa = 11.84 MLPRR40 pKa = 11.84 PCSRR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 HH49 pKa = 4.65 CRR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 WLRR56 pKa = 11.84 QPRR59 pKa = 11.84 RR60 pKa = 11.84 CQPRR64 pKa = 11.84 SPRR67 pKa = 11.84 RR68 pKa = 11.84 RR69 pKa = 11.84 TVPPRR74 pKa = 11.84 RR75 pKa = 11.84 PPASRR80 pKa = 11.84 RR81 pKa = 3.58
Molecular weight: 10.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.096
IPC_protein 12.72
Toseland 12.881
ProMoST 13.378
Dawson 12.881
Bjellqvist 12.881
Wikipedia 13.349
Rodwell 12.369
Grimsley 12.91
Solomon 13.378
Lehninger 13.276
Nozaki 12.881
DTASelect 12.881
Thurlkill 12.881
EMBOSS 13.378
Sillero 12.881
Patrickios 12.091
IPC_peptide 13.378
IPC2_peptide 12.369
IPC2.peptide.svr19 9.175
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4473
0
4473
1264196
42
2071
282.6
30.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.701 ± 0.047
0.737 ± 0.011
7.932 ± 0.05
8.006 ± 0.057
3.407 ± 0.024
8.66 ± 0.036
2.048 ± 0.019
3.544 ± 0.027
1.749 ± 0.024
9.097 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.715 ± 0.016
2.266 ± 0.02
4.761 ± 0.022
2.369 ± 0.02
7.009 ± 0.042
5.843 ± 0.032
6.629 ± 0.038
9.742 ± 0.045
1.161 ± 0.015
2.625 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here