Avian paramyxovirus 14
Average proteome isoelectric point is 5.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L5YIM1|A0A1L5YIM1_9MONO Isoform of A0A1L5YIL7 W protein OS=Avian paramyxovirus 14 OX=1928005 GN=P PE=4 SV=1
MM1 pKa = 7.93 DD2 pKa = 5.21 FSDD5 pKa = 4.51 DD6 pKa = 3.99 NEE8 pKa = 4.13 ITEE11 pKa = 4.89 LLNISLDD18 pKa = 3.99 TINKK22 pKa = 7.87 IQHH25 pKa = 6.47 AEE27 pKa = 4.15 DD28 pKa = 3.71 IKK30 pKa = 10.93 PKK32 pKa = 9.93 TVGKK36 pKa = 10.2 SAIKK40 pKa = 10.05 PGNTKK45 pKa = 10.64 ALTKK49 pKa = 10.38 AWEE52 pKa = 4.64 DD53 pKa = 3.76 DD54 pKa = 4.09 SNTTSDD60 pKa = 3.07 NTQNDD65 pKa = 3.08 VRR67 pKa = 11.84 GNSSAASNAQQSKK80 pKa = 10.21 EE81 pKa = 3.88 EE82 pKa = 4.38 SGPEE86 pKa = 3.96 TRR88 pKa = 11.84 PEE90 pKa = 3.99 TAGVEE95 pKa = 4.53 VPSDD99 pKa = 3.76 PTKK102 pKa = 10.82 SSPDD106 pKa = 3.27 QAEE109 pKa = 4.5 TGWSRR114 pKa = 11.84 GSNLDD119 pKa = 3.18 KK120 pKa = 10.82 TLEE123 pKa = 4.07 NLEE126 pKa = 4.32 KK127 pKa = 10.07 ATSNRR132 pKa = 11.84 VSDD135 pKa = 3.61 QTQKK139 pKa = 10.51 VLKK142 pKa = 8.93 GGPVARR148 pKa = 11.84 DD149 pKa = 3.14 PLLRR153 pKa = 11.84 RR154 pKa = 11.84 PRR156 pKa = 3.81
Molecular weight: 16.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.123
IPC2_protein 5.029
IPC_protein 4.902
Toseland 4.749
ProMoST 5.016
Dawson 4.851
Bjellqvist 5.003
Wikipedia 4.724
Rodwell 4.749
Grimsley 4.66
Solomon 4.851
Lehninger 4.8
Nozaki 4.965
DTASelect 5.13
Thurlkill 4.749
EMBOSS 4.749
Sillero 5.016
Patrickios 4.838
IPC_peptide 4.851
IPC2_peptide 5.003
IPC2.peptide.svr19 5.055
Protein with the highest isoelectric point:
>tr|A0A1L5YIM9|A0A1L5YIM9_9MONO RNA-directed RNA polymerase L OS=Avian paramyxovirus 14 OX=1928005 GN=L PE=3 SV=1
MM1 pKa = 7.63 ASASVNLFVDD11 pKa = 4.87 EE12 pKa = 4.62 SAPSSSLLAFPIVTKK27 pKa = 10.92 DD28 pKa = 3.08 NGSGGKK34 pKa = 9.57 ALQPQIRR41 pKa = 11.84 IAYY44 pKa = 8.16 HH45 pKa = 6.4 GEE47 pKa = 3.93 VQGSKK52 pKa = 9.79 RR53 pKa = 11.84 SVMFVNCYY61 pKa = 10.45 GFIEE65 pKa = 5.17 DD66 pKa = 3.83 YY67 pKa = 10.97 SDD69 pKa = 3.78 RR70 pKa = 11.84 NEE72 pKa = 4.2 GFLNPDD78 pKa = 3.64 EE79 pKa = 4.61 PPKK82 pKa = 10.58 PRR84 pKa = 11.84 TVTAACLTIGSVSGDD99 pKa = 3.39 LDD101 pKa = 3.49 PRR103 pKa = 11.84 EE104 pKa = 4.07 VARR107 pKa = 11.84 ACFDD111 pKa = 4.09 LDD113 pKa = 3.7 VKK115 pKa = 11.06 VKK117 pKa = 10.33 INADD121 pKa = 3.04 SRR123 pKa = 11.84 EE124 pKa = 3.66 RR125 pKa = 11.84 VAFSFRR131 pKa = 11.84 TKK133 pKa = 10.03 PALLISSRR141 pKa = 11.84 VISGGGIVLSAEE153 pKa = 4.33 DD154 pKa = 4.03 NIKK157 pKa = 10.74 CVGKK161 pKa = 9.01 MAVDD165 pKa = 3.03 KK166 pKa = 10.57 DD167 pKa = 3.48 YY168 pKa = 11.22 RR169 pKa = 11.84 LRR171 pKa = 11.84 IMFCSTTFLHH181 pKa = 6.39 QSKK184 pKa = 10.18 LFKK187 pKa = 10.57 AQPAVMNLRR196 pKa = 11.84 SSMLLALQVAVTIKK210 pKa = 10.38 VDD212 pKa = 3.37 IPASHH217 pKa = 7.11 PHH219 pKa = 6.15 AKK221 pKa = 10.07 YY222 pKa = 10.38 LSKK225 pKa = 11.23 EE226 pKa = 3.84 GDD228 pKa = 3.7 DD229 pKa = 4.25 LVAHH233 pKa = 6.07 VWIHH237 pKa = 4.34 VCNFKK242 pKa = 10.04 RR243 pKa = 11.84 TDD245 pKa = 3.1 RR246 pKa = 11.84 RR247 pKa = 11.84 GEE249 pKa = 3.91 TRR251 pKa = 11.84 TVEE254 pKa = 3.92 NLKK257 pKa = 10.57 EE258 pKa = 4.1 KK259 pKa = 9.69 VRR261 pKa = 11.84 RR262 pKa = 11.84 MGLKK266 pKa = 10.33 VSLLDD271 pKa = 3.29 LWGPTIIIEE280 pKa = 4.34 ATGTMTKK287 pKa = 10.22 YY288 pKa = 11.05 ALGFFSDD295 pKa = 4.57 SKK297 pKa = 9.69 TSCHH301 pKa = 6.82 PISKK305 pKa = 10.19 ASPEE309 pKa = 4.15 TAKK312 pKa = 10.94 LLWSATGEE320 pKa = 4.25 VKK322 pKa = 10.57 RR323 pKa = 11.84 AVAVIQSSDD332 pKa = 2.88 KK333 pKa = 10.75 RR334 pKa = 11.84 ALLTEE339 pKa = 5.04 DD340 pKa = 3.92 DD341 pKa = 4.77 LLVKK345 pKa = 10.53 GAMTTQAGGLKK356 pKa = 10.03 EE357 pKa = 3.85 FSLFKK362 pKa = 10.73 KK363 pKa = 10.56
Molecular weight: 39.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.72
IPC2_protein 8.463
IPC_protein 8.346
Toseland 9.209
ProMoST 9.019
Dawson 9.443
Bjellqvist 9.268
Wikipedia 9.545
Rodwell 9.823
Grimsley 9.428
Solomon 9.516
Lehninger 9.502
Nozaki 9.531
DTASelect 9.151
Thurlkill 9.37
EMBOSS 9.648
Sillero 9.502
Patrickios 5.474
IPC_peptide 9.516
IPC2_peptide 7.907
IPC2.peptide.svr19 7.828
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
2
8
5006
156
2243
625.8
69.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.752 ± 0.655
2.058 ± 0.313
5.453 ± 0.382
5.733 ± 0.255
3.256 ± 0.422
6.312 ± 0.294
1.578 ± 0.227
7.231 ± 0.63
5.773 ± 0.351
9.409 ± 0.824
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.277 ± 0.323
4.914 ± 0.449
4.095 ± 0.408
4.055 ± 0.307
4.874 ± 0.409
9.189 ± 0.239
6.792 ± 0.592
6.153 ± 0.385
1.179 ± 0.231
2.917 ± 0.434
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here