Luteimonas granuli
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2469 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A518N6G5|A0A518N6G5_9GAMM AMP-binding protein OS=Luteimonas granuli OX=1176533 GN=FPZ22_11900 PE=4 SV=1
MM1 pKa = 7.52 NLNKK5 pKa = 10.23 LAIAAALAITATGVQAADD23 pKa = 3.4 TTGTFTVSTNVTASCEE39 pKa = 4.04 VSATDD44 pKa = 5.42 LVFTDD49 pKa = 4.09 VDD51 pKa = 3.86 PLANATTATDD61 pKa = 3.64 GDD63 pKa = 4.09 TTISVTCSNTTPYY76 pKa = 11.03 NVGLSVGSGAGATVAARR93 pKa = 11.84 KK94 pKa = 6.85 MTHH97 pKa = 6.38 TDD99 pKa = 2.95 TTSTLDD105 pKa = 3.3 YY106 pKa = 11.29 ALFSDD111 pKa = 5.41 AGRR114 pKa = 11.84 TTDD117 pKa = 2.75 WGHH120 pKa = 5.65 TVSTDD125 pKa = 3.49 TVPGTGTGVAQSLTVYY141 pKa = 10.21 GQIASGQEE149 pKa = 3.87 TAKK152 pKa = 10.74 VGAYY156 pKa = 8.55 TDD158 pKa = 4.3 TITVTVTYY166 pKa = 10.89
Molecular weight: 16.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.775
IPC2_protein 3.986
IPC_protein 3.948
Toseland 3.719
ProMoST 4.151
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.961
Rodwell 3.77
Grimsley 3.63
Solomon 3.961
Lehninger 3.91
Nozaki 4.101
DTASelect 4.406
Thurlkill 3.795
EMBOSS 3.961
Sillero 4.075
Patrickios 0.896
IPC_peptide 3.948
IPC2_peptide 4.05
IPC2.peptide.svr19 3.962
Protein with the highest isoelectric point:
>tr|A0A518N2X1|A0A518N2X1_9GAMM AsmA family protein OS=Luteimonas granuli OX=1176533 GN=FPZ22_04590 PE=4 SV=1
MM1 pKa = 7.14 ATKK4 pKa = 10.21 KK5 pKa = 10.04 VAKK8 pKa = 9.95 KK9 pKa = 10.48 KK10 pKa = 8.84 PAKK13 pKa = 9.91 KK14 pKa = 9.74 AAKK17 pKa = 8.43 KK18 pKa = 6.97 TAKK21 pKa = 10.13 KK22 pKa = 10.02 AVRR25 pKa = 11.84 KK26 pKa = 8.68 AAPRR30 pKa = 11.84 KK31 pKa = 8.14 VAKK34 pKa = 10.45 KK35 pKa = 10.09 KK36 pKa = 8.97 VAKK39 pKa = 10.07 KK40 pKa = 9.63 AAKK43 pKa = 9.58 KK44 pKa = 8.97 ATRR47 pKa = 11.84 KK48 pKa = 8.43 VAKK51 pKa = 9.83 KK52 pKa = 10.08 AAVKK56 pKa = 10.44 KK57 pKa = 10.12 AVKK60 pKa = 9.97 KK61 pKa = 9.95 AAKK64 pKa = 8.52 KK65 pKa = 7.28 TAKK68 pKa = 9.05 KK69 pKa = 5.34 TTKK72 pKa = 10.33 KK73 pKa = 10.35 AVKK76 pKa = 9.9 KK77 pKa = 10.71 AGAKK81 pKa = 8.28 KK82 pKa = 7.86 TAKK85 pKa = 10.35 KK86 pKa = 8.98 ATKK89 pKa = 9.77 KK90 pKa = 9.51 VAKK93 pKa = 9.84 KK94 pKa = 10.45 RR95 pKa = 11.84 PAKK98 pKa = 10.05 KK99 pKa = 9.97 AAAKK103 pKa = 10.11 KK104 pKa = 8.98 ATAKK108 pKa = 9.12 KK109 pKa = 7.44 TAKK112 pKa = 10.14 KK113 pKa = 10.01 AVKK116 pKa = 9.72 KK117 pKa = 10.86 APAKK121 pKa = 10.15 KK122 pKa = 9.75 AAKK125 pKa = 8.57 KK126 pKa = 7.18 TAKK129 pKa = 10.16 KK130 pKa = 8.93 ATKK133 pKa = 8.78 KK134 pKa = 8.92 TVAKK138 pKa = 8.85 KK139 pKa = 8.17 TAAKK143 pKa = 8.63 KK144 pKa = 8.15 TATKK148 pKa = 8.98 KK149 pKa = 8.71 TATKK153 pKa = 10.1 KK154 pKa = 9.45 AAKK157 pKa = 9.64 KK158 pKa = 9.41 RR159 pKa = 11.84 APRR162 pKa = 11.84 KK163 pKa = 9.16 AKK165 pKa = 10.44 APVVSIPAAPAPLMM179 pKa = 4.49
Molecular weight: 18.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.382
IPC2_protein 11.345
IPC_protein 12.544
Toseland 12.866
ProMoST 13.217
Dawson 12.881
Bjellqvist 12.764
Wikipedia 13.261
Rodwell 13.393
Grimsley 12.91
Solomon 13.261
Lehninger 13.188
Nozaki 12.852
DTASelect 12.764
Thurlkill 12.852
EMBOSS 13.32
Sillero 12.852
Patrickios 13.1
IPC_peptide 13.276
IPC2_peptide 12.193
IPC2.peptide.svr19 8.874
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2469
0
2469
828099
41
2791
335.4
36.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.919 ± 0.079
0.82 ± 0.016
6.023 ± 0.041
5.619 ± 0.039
3.231 ± 0.031
9.285 ± 0.048
2.265 ± 0.023
3.862 ± 0.032
2.1 ± 0.039
10.733 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.216 ± 0.022
2.063 ± 0.03
5.591 ± 0.04
2.996 ± 0.027
8.549 ± 0.052
4.819 ± 0.034
4.584 ± 0.031
7.743 ± 0.04
1.513 ± 0.02
2.068 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here