Lactococcus phage bIL310

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9AZI9|Q9AZI9_9CAUD Orf20 OS=Lactococcus phage bIL310 OX=151538 GN=orf20 PE=4 SV=1
MM1 pKa = 7.84KK2 pKa = 9.41ITIDD6 pKa = 3.51VLEE9 pKa = 4.58NEE11 pKa = 4.49SNKK14 pKa = 10.85DD15 pKa = 3.29NLEE18 pKa = 3.92YY19 pKa = 10.73LISDD23 pKa = 4.41TSNEE27 pKa = 3.76AMTVLMFTLIGEE39 pKa = 4.39ARR41 pKa = 11.84QRR43 pKa = 11.84ASYY46 pKa = 9.96EE47 pKa = 3.94QFLEE51 pKa = 4.54TITRR55 pKa = 11.84IWGYY59 pKa = 11.07LNEE62 pKa = 4.85DD63 pKa = 3.28NN64 pKa = 5.38

Molecular weight: 7.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9AZK5|Q9AZK5_9CAUD Orf4 OS=Lactococcus phage bIL310 OX=151538 GN=orf4 PE=4 SV=1
MM1 pKa = 7.3FGINRR6 pKa = 11.84LYY8 pKa = 10.8NIFYY12 pKa = 10.46NLYY15 pKa = 9.39IKK17 pKa = 10.69KK18 pKa = 9.22PLQRR22 pKa = 11.84VTLYY26 pKa = 10.58LKK28 pKa = 10.98VMTSQNHH35 pKa = 4.2YY36 pKa = 9.65TFILHH41 pKa = 6.48FFVFGFKK48 pKa = 10.31LRR50 pKa = 11.84NRR52 pKa = 11.84DD53 pKa = 3.38MVVTSFICNYY63 pKa = 9.49LRR65 pKa = 11.84SHH67 pKa = 6.78FTTLANRR74 pKa = 11.84SKK76 pKa = 10.59IPPIKK81 pKa = 10.27LLWVV85 pKa = 3.64

Molecular weight: 10.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

28

0

28

3788

28

542

135.3

15.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.359 ± 0.372

0.818 ± 0.241

6.019 ± 0.433

7.233 ± 0.527

4.567 ± 0.404

4.91 ± 0.358

1.795 ± 0.195

7.973 ± 0.675

9.029 ± 0.692

10.375 ± 0.518

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.27 ± 0.258

5.94 ± 0.344

3.036 ± 0.38

3.511 ± 0.261

5.148 ± 0.41

5.781 ± 0.489

6.257 ± 0.417

5.412 ± 0.624

0.95 ± 0.202

3.617 ± 0.356
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski