Amino acid dipepetide frequency for Lactococcus phage bIL310

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.0AlaAla: 0.0 ± 0.0
0.528AlaCys: 0.528 ± 0.362
2.905AlaAsp: 2.905 ± 0.908
6.073AlaGlu: 6.073 ± 1.183
2.905AlaPhe: 2.905 ± 0.927
2.641AlaGly: 2.641 ± 0.665
0.792AlaHis: 0.792 ± 0.466
4.489AlaIle: 4.489 ± 1.052
4.753AlaLys: 4.753 ± 0.871
6.602AlaLeu: 6.602 ± 1.492
2.112AlaMet: 2.112 ± 0.558
2.905AlaAsn: 2.905 ± 0.615
0.528AlaPro: 0.528 ± 0.277
1.056AlaGln: 1.056 ± 0.492
3.169AlaArg: 3.169 ± 0.776
3.169AlaSer: 3.169 ± 1.041
2.905AlaThr: 2.905 ± 0.792
3.433AlaVal: 3.433 ± 1.08
1.056AlaTrp: 1.056 ± 0.355
1.584AlaTyr: 1.584 ± 0.677
0.0AlaXaa: 0.0 ± 0.0
Cys
0.528CysAla: 0.528 ± 0.359
0.264CysCys: 0.264 ± 0.224
1.056CysAsp: 1.056 ± 0.534
0.528CysGlu: 0.528 ± 0.293
0.0CysPhe: 0.0 ± 0.0
0.264CysGly: 0.264 ± 0.216
0.264CysHis: 0.264 ± 0.224
1.32CysIle: 1.32 ± 0.476
0.264CysLys: 0.264 ± 0.238
0.264CysLeu: 0.264 ± 0.218
0.264CysMet: 0.264 ± 0.228
0.528CysAsn: 0.528 ± 0.34
0.528CysPro: 0.528 ± 0.339
0.528CysGln: 0.528 ± 0.29
0.264CysArg: 0.264 ± 0.235
0.264CysSer: 0.264 ± 0.275
0.264CysThr: 0.264 ± 0.291
0.264CysVal: 0.264 ± 0.235
0.264CysTrp: 0.264 ± 0.224
0.264CysTyr: 0.264 ± 0.278
0.0CysXaa: 0.0 ± 0.0
Asp
0.792AspAla: 0.792 ± 0.411
0.264AspCys: 0.264 ± 0.228
4.753AspAsp: 4.753 ± 0.978
5.017AspGlu: 5.017 ± 0.992
3.169AspPhe: 3.169 ± 0.928
3.169AspGly: 3.169 ± 0.843
0.528AspHis: 0.528 ± 0.293
4.489AspIle: 4.489 ± 1.063
4.489AspLys: 4.489 ± 0.91
5.545AspLeu: 5.545 ± 1.436
1.32AspMet: 1.32 ± 0.473
5.281AspAsn: 5.281 ± 0.907
0.528AspPro: 0.528 ± 0.332
1.32AspGln: 1.32 ± 0.689
2.377AspArg: 2.377 ± 0.761
5.017AspSer: 5.017 ± 0.924
3.433AspThr: 3.433 ± 1.091
2.641AspVal: 2.641 ± 0.74
1.848AspTrp: 1.848 ± 0.539
4.225AspTyr: 4.225 ± 0.858
0.0AspXaa: 0.0 ± 0.0
Glu
2.905GluAla: 2.905 ± 0.735
0.792GluCys: 0.792 ± 0.474
5.017GluAsp: 5.017 ± 1.273
3.697GluGlu: 3.697 ± 1.005
1.32GluPhe: 1.32 ± 0.55
2.641GluGly: 2.641 ± 0.907
1.848GluHis: 1.848 ± 0.559
5.017GluIle: 5.017 ± 1.172
7.394GluLys: 7.394 ± 1.172
9.77GluLeu: 9.77 ± 1.565
3.169GluMet: 3.169 ± 0.937
3.961GluAsn: 3.961 ± 1.092
2.377GluPro: 2.377 ± 0.774
3.169GluGln: 3.169 ± 0.812
4.225GluArg: 4.225 ± 0.825
4.489GluSer: 4.489 ± 1.29
3.697GluThr: 3.697 ± 0.85
3.169GluVal: 3.169 ± 0.715
0.0GluTrp: 0.0 ± 0.0
3.697GluTyr: 3.697 ± 0.869
0.0GluXaa: 0.0 ± 0.0
Phe
2.905PheAla: 2.905 ± 0.902
0.528PheCys: 0.528 ± 0.349
3.961PheAsp: 3.961 ± 0.876
5.017PheGlu: 5.017 ± 1.073
1.584PhePhe: 1.584 ± 0.498
1.32PheGly: 1.32 ± 0.755
0.792PheHis: 0.792 ± 0.373
4.489PheIle: 4.489 ± 1.525
4.489PheLys: 4.489 ± 0.835
2.905PheLeu: 2.905 ± 0.83
1.056PheMet: 1.056 ± 0.521
3.433PheAsn: 3.433 ± 0.822
0.528PhePro: 0.528 ± 0.354
0.528PheGln: 0.528 ± 0.31
2.112PheArg: 2.112 ± 0.639
2.112PheSer: 2.112 ± 0.784
3.961PheThr: 3.961 ± 0.95
2.112PheVal: 2.112 ± 0.969
0.0PheTrp: 0.0 ± 0.0
1.584PheTyr: 1.584 ± 0.648
0.0PheXaa: 0.0 ± 0.0
Gly
2.112GlyAla: 2.112 ± 0.648
0.792GlyCys: 0.792 ± 0.392
2.112GlyAsp: 2.112 ± 0.779
3.697GlyGlu: 3.697 ± 0.965
2.377GlyPhe: 2.377 ± 0.505
2.377GlyGly: 2.377 ± 0.763
0.264GlyHis: 0.264 ± 0.235
4.489GlyIle: 4.489 ± 1.185
4.753GlyLys: 4.753 ± 1.333
5.281GlyLeu: 5.281 ± 1.135
1.056GlyMet: 1.056 ± 0.518
3.961GlyAsn: 3.961 ± 0.936
0.0GlyPro: 0.0 ± 0.0
1.056GlyGln: 1.056 ± 0.512
1.848GlyArg: 1.848 ± 0.646
1.32GlySer: 1.32 ± 0.535
2.905GlyThr: 2.905 ± 0.679
3.961GlyVal: 3.961 ± 0.931
0.792GlyTrp: 0.792 ± 0.45
3.961GlyTyr: 3.961 ± 0.971
0.0GlyXaa: 0.0 ± 0.0
His
1.848HisAla: 1.848 ± 0.891
0.0HisCys: 0.0 ± 0.0
0.0HisAsp: 0.0 ± 0.0
1.584HisGlu: 1.584 ± 0.784
1.848HisPhe: 1.848 ± 0.811
0.792HisGly: 0.792 ± 0.407
0.528HisHis: 0.528 ± 0.376
0.792HisIle: 0.792 ± 0.551
0.792HisLys: 0.792 ± 0.428
1.584HisLeu: 1.584 ± 0.582
0.0HisMet: 0.0 ± 0.0
1.32HisAsn: 1.32 ± 0.618
0.528HisPro: 0.528 ± 0.362
0.792HisGln: 0.792 ± 0.348
1.584HisArg: 1.584 ± 0.708
1.056HisSer: 1.056 ± 0.336
1.056HisThr: 1.056 ± 0.418
0.528HisVal: 0.528 ± 0.364
0.264HisTrp: 0.264 ± 0.235
1.056HisTyr: 1.056 ± 0.502
0.0HisXaa: 0.0 ± 0.0
Ile
4.753IleAla: 4.753 ± 0.998
0.792IleCys: 0.792 ± 0.443
7.13IleAsp: 7.13 ± 1.671
3.961IleGlu: 3.961 ± 1.085
3.433IlePhe: 3.433 ± 1.031
4.489IleGly: 4.489 ± 0.787
1.056IleHis: 1.056 ± 0.59
6.073IleIle: 6.073 ± 1.007
10.562IleLys: 10.562 ± 1.281
6.602IleLeu: 6.602 ± 1.46
1.056IleMet: 1.056 ± 0.348
4.489IleAsn: 4.489 ± 0.927
2.905IlePro: 2.905 ± 0.875
2.641IleGln: 2.641 ± 0.679
2.112IleArg: 2.112 ± 0.805
4.753IleSer: 4.753 ± 1.003
5.809IleThr: 5.809 ± 1.539
4.225IleVal: 4.225 ± 1.19
0.528IleTrp: 0.528 ± 0.362
2.377IleTyr: 2.377 ± 0.736
0.0IleXaa: 0.0 ± 0.0
Lys
6.073LysAla: 6.073 ± 1.223
0.0LysCys: 0.0 ± 0.0
6.602LysAsp: 6.602 ± 1.135
6.866LysGlu: 6.866 ± 1.21
2.905LysPhe: 2.905 ± 0.843
5.281LysGly: 5.281 ± 1.012
2.377LysHis: 2.377 ± 0.746
6.073LysIle: 6.073 ± 1.056
7.922LysLys: 7.922 ± 1.691
6.602LysLeu: 6.602 ± 1.356
3.433LysMet: 3.433 ± 0.99
3.433LysAsn: 3.433 ± 1.161
3.697LysPro: 3.697 ± 0.838
5.281LysGln: 5.281 ± 1.171
4.753LysArg: 4.753 ± 1.195
3.961LysSer: 3.961 ± 0.767
6.866LysThr: 6.866 ± 0.998
5.017LysVal: 5.017 ± 1.454
0.264LysTrp: 0.264 ± 0.235
2.905LysTyr: 2.905 ± 0.572
0.0LysXaa: 0.0 ± 0.0
Leu
6.073LeuAla: 6.073 ± 1.294
1.848LeuCys: 1.848 ± 0.653
5.809LeuAsp: 5.809 ± 1.183
6.337LeuGlu: 6.337 ± 1.593
5.017LeuPhe: 5.017 ± 1.356
6.337LeuGly: 6.337 ± 1.345
1.584LeuHis: 1.584 ± 0.607
7.922LeuIle: 7.922 ± 1.11
6.602LeuLys: 6.602 ± 1.206
8.978LeuLeu: 8.978 ± 1.662
2.905LeuMet: 2.905 ± 0.761
6.602LeuAsn: 6.602 ± 1.287
4.225LeuPro: 4.225 ± 1.107
2.905LeuGln: 2.905 ± 0.967
5.545LeuArg: 5.545 ± 0.965
7.922LeuSer: 7.922 ± 1.226
7.922LeuThr: 7.922 ± 2.023
4.225LeuVal: 4.225 ± 0.888
1.32LeuTrp: 1.32 ± 0.413
3.697LeuTyr: 3.697 ± 1.126
0.0LeuXaa: 0.0 ± 0.0
Met
0.528MetAla: 0.528 ± 0.342
0.528MetCys: 0.528 ± 0.341
1.056MetAsp: 1.056 ± 0.467
2.112MetGlu: 2.112 ± 0.853
1.584MetPhe: 1.584 ± 0.627
0.264MetGly: 0.264 ± 0.275
0.264MetHis: 0.264 ± 0.224
2.641MetIle: 2.641 ± 0.97
3.433MetLys: 3.433 ± 1.257
0.792MetLeu: 0.792 ± 0.386
0.0MetMet: 0.0 ± 0.0
2.377MetAsn: 2.377 ± 0.625
0.264MetPro: 0.264 ± 0.264
0.792MetGln: 0.792 ± 0.373
0.792MetArg: 0.792 ± 0.464
1.056MetSer: 1.056 ± 0.427
2.905MetThr: 2.905 ± 0.734
1.056MetVal: 1.056 ± 0.543
0.264MetTrp: 0.264 ± 0.228
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
3.697AsnAla: 3.697 ± 0.929
0.528AsnCys: 0.528 ± 0.381
2.905AsnAsp: 2.905 ± 0.986
5.809AsnGlu: 5.809 ± 1.083
2.377AsnPhe: 2.377 ± 0.927
5.545AsnGly: 5.545 ± 1.14
1.056AsnHis: 1.056 ± 0.494
5.017AsnIle: 5.017 ± 1.11
5.017AsnLys: 5.017 ± 0.961
7.658AsnLeu: 7.658 ± 1.694
0.264AsnMet: 0.264 ± 0.263
1.848AsnAsn: 1.848 ± 0.825
1.848AsnPro: 1.848 ± 0.566
1.848AsnGln: 1.848 ± 0.583
4.225AsnArg: 4.225 ± 1.274
2.905AsnSer: 2.905 ± 0.795
2.112AsnThr: 2.112 ± 0.831
2.905AsnVal: 2.905 ± 1.042
0.264AsnTrp: 0.264 ± 0.238
1.584AsnTyr: 1.584 ± 0.56
0.0AsnXaa: 0.0 ± 0.0
Pro
1.056ProAla: 1.056 ± 0.45
0.264ProCys: 0.264 ± 0.235
1.848ProAsp: 1.848 ± 0.728
0.792ProGlu: 0.792 ± 0.389
2.112ProPhe: 2.112 ± 0.666
0.264ProGly: 0.264 ± 0.263
0.792ProHis: 0.792 ± 0.486
1.848ProIle: 1.848 ± 0.663
4.753ProLys: 4.753 ± 1.454
5.281ProLeu: 5.281 ± 1.27
0.528ProMet: 0.528 ± 0.419
1.848ProAsn: 1.848 ± 0.488
0.528ProPro: 0.528 ± 0.374
1.848ProGln: 1.848 ± 0.565
0.792ProArg: 0.792 ± 0.37
1.056ProSer: 1.056 ± 0.407
2.112ProThr: 2.112 ± 0.561
1.584ProVal: 1.584 ± 0.639
0.0ProTrp: 0.0 ± 0.0
1.056ProTyr: 1.056 ± 0.459
0.0ProXaa: 0.0 ± 0.0
Gln
2.905GlnAla: 2.905 ± 0.699
0.0GlnCys: 0.0 ± 0.0
1.32GlnAsp: 1.32 ± 0.537
3.697GlnGlu: 3.697 ± 0.866
1.848GlnPhe: 1.848 ± 0.641
1.584GlnGly: 1.584 ± 0.595
1.056GlnHis: 1.056 ± 0.447
2.377GlnIle: 2.377 ± 0.658
2.112GlnLys: 2.112 ± 0.928
2.905GlnLeu: 2.905 ± 0.636
0.0GlnMet: 0.0 ± 0.0
1.848GlnAsn: 1.848 ± 0.702
0.792GlnPro: 0.792 ± 0.339
1.32GlnGln: 1.32 ± 0.555
2.641GlnArg: 2.641 ± 0.678
2.377GlnSer: 2.377 ± 0.688
2.112GlnThr: 2.112 ± 0.791
3.433GlnVal: 3.433 ± 0.74
0.528GlnTrp: 0.528 ± 0.31
0.264GlnTyr: 0.264 ± 0.216
0.0GlnXaa: 0.0 ± 0.0
Arg
5.809ArgAla: 5.809 ± 1.083
0.0ArgCys: 0.0 ± 0.0
1.056ArgAsp: 1.056 ± 0.458
2.905ArgGlu: 2.905 ± 0.896
2.377ArgPhe: 2.377 ± 0.649
1.32ArgGly: 1.32 ± 0.611
0.528ArgHis: 0.528 ± 0.391
4.489ArgIle: 4.489 ± 1.082
5.281ArgLys: 5.281 ± 1.147
6.073ArgLeu: 6.073 ± 1.205
0.528ArgMet: 0.528 ± 0.382
3.169ArgAsn: 3.169 ± 0.95
1.32ArgPro: 1.32 ± 0.544
1.32ArgGln: 1.32 ± 0.601
3.169ArgArg: 3.169 ± 0.848
1.848ArgSer: 1.848 ± 0.745
4.225ArgThr: 4.225 ± 1.237
3.697ArgVal: 3.697 ± 1.299
0.264ArgTrp: 0.264 ± 0.253
2.112ArgTyr: 2.112 ± 0.854
0.0ArgXaa: 0.0 ± 0.0
Ser
2.641SerAla: 2.641 ± 0.748
0.528SerCys: 0.528 ± 0.355
3.433SerAsp: 3.433 ± 0.81
5.281SerGlu: 5.281 ± 1.446
2.641SerPhe: 2.641 ± 0.861
2.641SerGly: 2.641 ± 0.671
2.112SerHis: 2.112 ± 0.677
4.225SerIle: 4.225 ± 0.983
3.697SerLys: 3.697 ± 0.673
5.809SerLeu: 5.809 ± 1.137
0.792SerMet: 0.792 ± 0.435
2.905SerAsn: 2.905 ± 0.693
3.169SerPro: 3.169 ± 0.791
2.641SerGln: 2.641 ± 0.661
4.225SerArg: 4.225 ± 0.96
2.112SerSer: 2.112 ± 0.865
3.169SerThr: 3.169 ± 0.821
3.697SerVal: 3.697 ± 0.776
0.528SerTrp: 0.528 ± 0.373
1.584SerTyr: 1.584 ± 0.708
0.0SerXaa: 0.0 ± 0.0
Thr
3.961ThrAla: 3.961 ± 0.918
0.264ThrCys: 0.264 ± 0.218
3.961ThrAsp: 3.961 ± 0.748
4.225ThrGlu: 4.225 ± 1.122
2.377ThrPhe: 2.377 ± 0.671
4.225ThrGly: 4.225 ± 1.033
1.056ThrHis: 1.056 ± 0.464
3.697ThrIle: 3.697 ± 1.129
4.225ThrLys: 4.225 ± 1.002
9.242ThrLeu: 9.242 ± 1.77
1.056ThrMet: 1.056 ± 0.569
3.961ThrAsn: 3.961 ± 0.915
3.697ThrPro: 3.697 ± 0.798
3.961ThrGln: 3.961 ± 1.179
1.584ThrArg: 1.584 ± 0.617
3.961ThrSer: 3.961 ± 1.081
2.377ThrThr: 2.377 ± 0.635
2.905ThrVal: 2.905 ± 0.651
0.264ThrTrp: 0.264 ± 0.285
1.584ThrTyr: 1.584 ± 0.576
0.0ThrXaa: 0.0 ± 0.0
Val
4.753ValAla: 4.753 ± 0.936
0.0ValCys: 0.0 ± 0.0
3.169ValAsp: 3.169 ± 0.602
2.112ValGlu: 2.112 ± 0.914
2.905ValPhe: 2.905 ± 0.824
2.112ValGly: 2.112 ± 0.548
0.0ValHis: 0.0 ± 0.0
5.809ValIle: 5.809 ± 1.09
5.809ValLys: 5.809 ± 1.18
5.545ValLeu: 5.545 ± 1.183
1.32ValMet: 1.32 ± 0.708
2.641ValAsn: 2.641 ± 0.945
1.848ValPro: 1.848 ± 0.687
1.32ValGln: 1.32 ± 0.502
2.905ValArg: 2.905 ± 0.956
5.809ValSer: 5.809 ± 0.671
1.848ValThr: 1.848 ± 0.857
2.641ValVal: 2.641 ± 0.937
0.264ValTrp: 0.264 ± 0.218
1.32ValTyr: 1.32 ± 0.603
0.0ValXaa: 0.0 ± 0.0
Trp
0.0TrpAla: 0.0 ± 0.0
0.264TrpCys: 0.264 ± 0.218
0.792TrpAsp: 0.792 ± 0.384
1.32TrpGlu: 1.32 ± 0.469
1.056TrpPhe: 1.056 ± 0.505
0.264TrpGly: 0.264 ± 0.264
0.0TrpHis: 0.0 ± 0.0
0.528TrpIle: 0.528 ± 0.359
1.056TrpLys: 1.056 ± 0.453
1.848TrpLeu: 1.848 ± 0.823
0.264TrpMet: 0.264 ± 0.235
0.528TrpAsn: 0.528 ± 0.265
0.0TrpPro: 0.0 ± 0.0
0.264TrpGln: 0.264 ± 0.218
0.264TrpArg: 0.264 ± 0.228
0.264TrpSer: 0.264 ± 0.235
0.264TrpThr: 0.264 ± 0.252
0.264TrpVal: 0.264 ± 0.296
0.0TrpTrp: 0.0 ± 0.0
0.264TrpTyr: 0.264 ± 0.285
0.0TrpXaa: 0.0 ± 0.0
Tyr
0.264TyrAla: 0.264 ± 0.236
0.0TyrCys: 0.0 ± 0.0
1.32TyrAsp: 1.32 ± 0.756
1.32TyrGlu: 1.32 ± 0.432
1.848TyrPhe: 1.848 ± 0.567
1.584TyrGly: 1.584 ± 0.524
1.056TyrHis: 1.056 ± 0.49
3.961TyrIle: 3.961 ± 0.966
2.905TyrLys: 2.905 ± 0.872
4.489TyrLeu: 4.489 ± 1.186
1.32TyrMet: 1.32 ± 0.61
2.377TyrAsn: 2.377 ± 1.005
1.056TyrPro: 1.056 ± 0.595
0.528TyrGln: 0.528 ± 0.31
2.905TyrArg: 2.905 ± 0.883
2.377TyrSer: 2.377 ± 0.809
2.641TyrThr: 2.641 ± 0.748
2.377TyrVal: 2.377 ± 0.843
0.792TyrTrp: 0.792 ± 0.429
1.056TyrTyr: 1.056 ± 0.625
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 28 proteins (3788 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski