Avian metaavulavirus 11
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I6UTP1|I6UTP1_9MONO Matrix protein OS=Avian metaavulavirus 11 OX=2560310 PE=4 SV=1
MM1 pKa = 6.99 GTCLNNRR8 pKa = 11.84 LSTIPSIKK16 pKa = 9.42 TVQCILIILSYY27 pKa = 10.14 IIPYY31 pKa = 9.91 SATDD35 pKa = 3.34 NPIADD40 pKa = 4.15 RR41 pKa = 11.84 SLLRR45 pKa = 11.84 AGIVPIYY52 pKa = 10.31 SKK54 pKa = 11.02 SLSVYY59 pKa = 8.18 TNSISGYY66 pKa = 7.89 LTVRR70 pKa = 11.84 MLPPLPKK77 pKa = 10.47 NLTEE81 pKa = 4.47 CSQEE85 pKa = 4.08 VVSNYY90 pKa = 10.36 NKK92 pKa = 9.99 TITRR96 pKa = 11.84 MFQPISDD103 pKa = 3.49 NLMRR107 pKa = 11.84 IQEE110 pKa = 4.36 GTDD113 pKa = 2.97 SGTKK117 pKa = 9.68 RR118 pKa = 11.84 FVGAVIGSVALGVATSAQITAALAMVQAQDD148 pKa = 3.13 NAKK151 pKa = 10.49 AIWKK155 pKa = 9.12 LKK157 pKa = 9.54 EE158 pKa = 4.72 AISSTNQAVLEE169 pKa = 4.25 LKK171 pKa = 10.69 EE172 pKa = 4.24 GVNTLGVAVDD182 pKa = 4.72 KK183 pKa = 11.03 IQGYY187 pKa = 9.6 INNEE191 pKa = 3.71 ILPSLSEE198 pKa = 4.01 LEE200 pKa = 4.38 CRR202 pKa = 11.84 VNANKK207 pKa = 10.11 LASQLNLYY215 pKa = 10.13 LIEE218 pKa = 4.33 LTTIFGDD225 pKa = 4.07 QITNPALTPLSLQALYY241 pKa = 9.87 TLAGDD246 pKa = 3.79 TMGSFLQYY254 pKa = 10.26 IGAQDD259 pKa = 3.69 NEE261 pKa = 4.34 IEE263 pKa = 4.19 SLYY266 pKa = 11.1 DD267 pKa = 3.08 SGLINGQIVSYY278 pKa = 10.07 DD279 pKa = 3.37 ASIQTIIIKK288 pKa = 10.22 VSIPSISSLSRR299 pKa = 11.84 FSIMRR304 pKa = 11.84 LATVSSSVGGFEE316 pKa = 4.04 KK317 pKa = 10.48 TPLVPEE323 pKa = 4.08 YY324 pKa = 11.25 LLISDD329 pKa = 3.47 NHH331 pKa = 7.44 IEE333 pKa = 4.05 EE334 pKa = 4.56 FSIVDD339 pKa = 3.87 CKK341 pKa = 10.73 EE342 pKa = 3.81 SSDD345 pKa = 4.44 IFYY348 pKa = 10.58 CPQILSMPISTATVEE363 pKa = 4.23 CLKK366 pKa = 10.95 GRR368 pKa = 11.84 IDD370 pKa = 3.36 QCIYY374 pKa = 10.36 TSQLTILSHH383 pKa = 7.66 RR384 pKa = 11.84 IVTYY388 pKa = 10.57 NGVVVANCFAEE399 pKa = 4.64 LCRR402 pKa = 11.84 CTNPSYY408 pKa = 10.72 IIRR411 pKa = 11.84 QDD413 pKa = 2.82 RR414 pKa = 11.84 DD415 pKa = 3.47 VAVTVIDD422 pKa = 3.68 KK423 pKa = 10.27 DD424 pKa = 3.48 LCKK427 pKa = 10.02 RR428 pKa = 11.84 VQIGDD433 pKa = 3.39 IEE435 pKa = 5.53 LIVQASIANEE445 pKa = 3.87 YY446 pKa = 9.83 KK447 pKa = 10.9 VNFTVSEE454 pKa = 4.15 DD455 pKa = 3.45 QLAPSTPIDD464 pKa = 3.36 ISNEE468 pKa = 3.91 LNSLNQTLDD477 pKa = 3.18 KK478 pKa = 10.94 VGQLINTSNQILASLNPKK496 pKa = 9.48 LVNNTSIIVLIVMGVVLILWLLALTIYY523 pKa = 10.47 SIYY526 pKa = 10.59 AARR529 pKa = 11.84 NLNSIGRR536 pKa = 11.84 LAKK539 pKa = 9.95 SAYY542 pKa = 9.76 ASYY545 pKa = 11.08 VADD548 pKa = 3.41 KK549 pKa = 10.96 NVYY552 pKa = 9.6 KK553 pKa = 10.93 NEE555 pKa = 4.23 STSSSSII562 pKa = 3.44
Molecular weight: 61.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.958
IPC2_protein 5.181
IPC_protein 5.092
Toseland 4.965
ProMoST 5.207
Dawson 5.054
Bjellqvist 5.194
Wikipedia 4.914
Rodwell 4.952
Grimsley 4.876
Solomon 5.054
Lehninger 5.003
Nozaki 5.156
DTASelect 5.321
Thurlkill 4.965
EMBOSS 4.94
Sillero 5.232
Patrickios 3.427
IPC_peptide 5.054
IPC2_peptide 5.219
IPC2.peptide.svr19 5.24
Protein with the highest isoelectric point:
>tr|I6UTP6|I6UTP6_9MONO Phosphoprotein OS=Avian metaavulavirus 11 OX=2560310 PE=4 SV=1
MM1 pKa = 7.27 SLDD4 pKa = 3.69 HH5 pKa = 6.44 PTEE8 pKa = 4.83 KK9 pKa = 10.39 INLPIPTGHH18 pKa = 7.73 PDD20 pKa = 3.48 LEE22 pKa = 4.35 LLAFPIIVEE31 pKa = 4.56 RR32 pKa = 11.84 GQDD35 pKa = 3.59 GQKK38 pKa = 9.1 TLQRR42 pKa = 11.84 QYY44 pKa = 11.13 RR45 pKa = 11.84 IGSVGDD51 pKa = 3.24 IFGSKK56 pKa = 7.67 EE57 pKa = 3.63 TGLFLTCYY65 pKa = 10.39 GFIEE69 pKa = 4.37 EE70 pKa = 5.54 SYY72 pKa = 10.86 TSTIQGKK79 pKa = 8.43 SRR81 pKa = 11.84 FFSEE85 pKa = 4.42 HH86 pKa = 5.53 QGGHH90 pKa = 5.9 PKK92 pKa = 9.92 IVTAALLPLGCVNPRR107 pKa = 11.84 EE108 pKa = 4.56 DD109 pKa = 3.62 IDD111 pKa = 4.87 RR112 pKa = 11.84 LIEE115 pKa = 4.16 DD116 pKa = 4.07 SYY118 pKa = 9.71 TLKK121 pKa = 11.02 VLISKK126 pKa = 10.25 NVDD129 pKa = 2.97 SKK131 pKa = 11.31 EE132 pKa = 3.8 RR133 pKa = 11.84 VVFKK137 pKa = 9.64 FVNKK141 pKa = 9.24 PAALSANKK149 pKa = 10.1 LLINGGVILDD159 pKa = 3.9 AEE161 pKa = 4.69 AFVKK165 pKa = 10.68 CPSKK169 pKa = 10.37 LTTNMEE175 pKa = 3.68 YY176 pKa = 10.3 SFRR179 pKa = 11.84 VVFVSVTRR187 pKa = 11.84 LDD189 pKa = 3.27 QTKK192 pKa = 10.06 LYY194 pKa = 9.93 RR195 pKa = 11.84 VNEE198 pKa = 4.54 SISKK202 pKa = 9.58 IRR204 pKa = 11.84 NPHH207 pKa = 6.53 LIAVNLEE214 pKa = 4.2 VKK216 pKa = 10.21 ILLHH220 pKa = 6.02 LTPDD224 pKa = 3.42 NPQLKK229 pKa = 9.98 FLIKK233 pKa = 10.05 HH234 pKa = 5.53 EE235 pKa = 4.41 KK236 pKa = 10.34 GGVGSVWIHH245 pKa = 6.17 LCNFAKK251 pKa = 10.64 KK252 pKa = 10.08 NMKK255 pKa = 8.72 GTLRR259 pKa = 11.84 SIQDD263 pKa = 3.05 VTEE266 pKa = 3.97 KK267 pKa = 9.93 VKK269 pKa = 10.9 RR270 pKa = 11.84 MALAVSLEE278 pKa = 4.25 DD279 pKa = 3.1 LWGPTVIVRR288 pKa = 11.84 ANGTMSKK295 pKa = 10.2 YY296 pKa = 10.93 ALGFFSTSKK305 pKa = 9.04 TACHH309 pKa = 7.04 PIYY312 pKa = 10.7 KK313 pKa = 9.94 LAPEE317 pKa = 4.07 IAKK320 pKa = 10.31 IMWSCSSEE328 pKa = 3.77 IIEE331 pKa = 5.13 ANIIVQGSMKK341 pKa = 10.56 NDD343 pKa = 2.97 ILTGSDD349 pKa = 3.74 LEE351 pKa = 4.44 IPSTSKK357 pKa = 10.41 IVNSGHH363 pKa = 5.73 KK364 pKa = 10.13 QFFSFKK370 pKa = 10.27 KK371 pKa = 10.2
Molecular weight: 41.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.698
IPC2_protein 8.492
IPC_protein 8.361
Toseland 9.238
ProMoST 9.033
Dawson 9.472
Bjellqvist 9.253
Wikipedia 9.589
Rodwell 9.867
Grimsley 9.487
Solomon 9.531
Lehninger 9.502
Nozaki 9.487
DTASelect 9.151
Thurlkill 9.37
EMBOSS 9.677
Sillero 9.487
Patrickios 5.258
IPC_peptide 9.531
IPC2_peptide 7.834
IPC2.peptide.svr19 7.797
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
4946
277
2251
706.6
79.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.803 ± 0.787
1.941 ± 0.294
5.156 ± 0.372
5.317 ± 0.324
3.255 ± 0.522
5.216 ± 0.383
2.082 ± 0.36
8.148 ± 0.69
4.873 ± 0.47
10.938 ± 0.949
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.123 ± 0.301
4.933 ± 0.323
4.792 ± 0.443
4.307 ± 0.396
5.176 ± 0.524
9.3 ± 0.597
6.692 ± 0.438
5.621 ± 0.472
1.072 ± 0.159
3.255 ± 0.385
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here