Olsenella sp. KH1P3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Coriobacteriales; Atopobiaceae; Olsenella; unclassified Olsenella

Average proteome isoelectric point is 5.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1609 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T4K3V7|A0A1T4K3V7_9ACTN Zinc transporter ZIP family OS=Olsenella sp. KH1P3 OX=1945884 GN=SAMN06298223_0023 PE=4 SV=1
WW1 pKa = 7.87DD2 pKa = 5.0EE3 pKa = 3.97IGGFQVIYY11 pKa = 10.61EE12 pKa = 4.57EE13 pKa = 5.3NNDD16 pKa = 4.06DD17 pKa = 4.88DD18 pKa = 4.33PTGVDD23 pKa = 4.66VDD25 pKa = 4.53DD26 pKa = 5.3LPNSVVTDD34 pKa = 3.93VKK36 pKa = 10.5WSTSGFDD43 pKa = 3.16TTYY46 pKa = 10.45VSGVAGGPTRR56 pKa = 11.84KK57 pKa = 10.34GYY59 pKa = 10.82DD60 pKa = 3.14FAGWFKK66 pKa = 11.06DD67 pKa = 3.32AGLTNPYY74 pKa = 10.58ASAEE78 pKa = 4.1AYY80 pKa = 9.33KK81 pKa = 10.79DD82 pKa = 3.82LVASTSVTSVTLYY95 pKa = 11.01AKK97 pKa = 9.85WDD99 pKa = 3.87VKK101 pKa = 11.04HH102 pKa = 6.76SDD104 pKa = 2.89AGVDD108 pKa = 3.31YY109 pKa = 10.89KK110 pKa = 11.25VVYY113 pKa = 10.16EE114 pKa = 4.52EE115 pKa = 4.94NNNDD119 pKa = 3.7DD120 pKa = 3.93PSGVDD125 pKa = 4.55VDD127 pKa = 5.03DD128 pKa = 5.17LPNSVVTVVDD138 pKa = 3.8WLTSGLDD145 pKa = 3.28TTFVSGVAGGPTRR158 pKa = 11.84QGYY161 pKa = 9.76AFAGWFKK168 pKa = 11.08DD169 pKa = 3.45AGLTQALASSDD180 pKa = 3.9TYY182 pKa = 11.62GGIAGDD188 pKa = 3.88DD189 pKa = 3.6TVTTVTLYY197 pKa = 11.15AKK199 pKa = 9.6WDD201 pKa = 3.64EE202 pKa = 4.08LADD205 pKa = 4.61FIIHH209 pKa = 6.39YY210 pKa = 9.55DD211 pKa = 3.58VNGGNVATTPADD223 pKa = 3.76RR224 pKa = 11.84TGVAWTDD231 pKa = 3.1VDD233 pKa = 5.09LKK235 pKa = 10.76PATAPTFAGRR245 pKa = 11.84HH246 pKa = 4.71LDD248 pKa = 3.78GYY250 pKa = 9.6GWAYY254 pKa = 10.5RR255 pKa = 11.84DD256 pKa = 3.31AAGNLVAISAATAYY270 pKa = 10.61SEE272 pKa = 4.42AWTLGQGAGKK282 pKa = 10.05EE283 pKa = 4.13VTLYY287 pKa = 11.16ALWADD292 pKa = 3.65NVYY295 pKa = 9.05TLHH298 pKa = 6.57YY299 pKa = 9.85DD300 pKa = 3.63SNGGQYY306 pKa = 11.13ADD308 pKa = 4.21GSTDD312 pKa = 3.27VPVVVKK318 pKa = 10.5WSDD321 pKa = 3.33SGFLTAAPAPTRR333 pKa = 11.84TGYY336 pKa = 9.82VLRR339 pKa = 11.84TTGAWNDD346 pKa = 3.54RR347 pKa = 11.84TDD349 pKa = 3.84GLGTALDD356 pKa = 5.16DD357 pKa = 4.04IMDD360 pKa = 4.02YY361 pKa = 11.27AALAGGNDD369 pKa = 3.62TVLDD373 pKa = 4.19VYY375 pKa = 11.14VYY377 pKa = 10.48AQWDD381 pKa = 4.15PITYY385 pKa = 9.26VVKK388 pKa = 10.76FNSNDD393 pKa = 3.19AAYY396 pKa = 6.93PTAEE400 pKa = 4.15AATGAMADD408 pKa = 3.23QTFTYY413 pKa = 10.24DD414 pKa = 2.92VSQALDD420 pKa = 3.49PNAFARR426 pKa = 11.84RR427 pKa = 11.84GYY429 pKa = 10.9SFAGWNTASDD439 pKa = 4.14GTGIAYY445 pKa = 8.75TDD447 pKa = 3.6AQSVLNLSSTDD458 pKa = 3.15GDD460 pKa = 3.88QIEE463 pKa = 5.6LFAIWDD469 pKa = 3.57

Molecular weight: 49.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T4MNZ0|A0A1T4MNZ0_9ACTN Ribonuclease P protein component OS=Olsenella sp. KH1P3 OX=1945884 GN=SAMN06298223_1109 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.62RR10 pKa = 11.84KK11 pKa = 9.56RR12 pKa = 11.84AKK14 pKa = 8.76THH16 pKa = 5.23GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.19AGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1609

0

1609

595354

37

13147

370.0

40.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.666 ± 0.098

1.481 ± 0.046

6.643 ± 0.052

6.382 ± 0.119

3.535 ± 0.037

8.536 ± 0.085

1.925 ± 0.029

4.792 ± 0.06

3.804 ± 0.065

9.025 ± 0.093

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.566 ± 0.036

3.009 ± 0.084

4.156 ± 0.036

2.896 ± 0.041

6.053 ± 0.113

6.631 ± 0.105

5.505 ± 0.176

8.374 ± 0.051

1.116 ± 0.068

2.904 ± 0.071
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski