Amino acid dipepetide frequency for Olsenella sp. KH1P3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.116AlaAla: 12.116 ± 0.245
1.702AlaCys: 1.702 ± 0.077
6.744AlaAsp: 6.744 ± 0.129
6.344AlaGlu: 6.344 ± 0.183
3.705AlaPhe: 3.705 ± 0.094
8.623AlaGly: 8.623 ± 0.166
2.118AlaHis: 2.118 ± 0.058
5.427AlaIle: 5.427 ± 0.127
4.241AlaLys: 4.241 ± 0.12
10.493AlaLeu: 10.493 ± 0.215
2.825AlaMet: 2.825 ± 0.088
3.116AlaAsn: 3.116 ± 0.109
3.946AlaPro: 3.946 ± 0.114
3.559AlaGln: 3.559 ± 0.085
7.024AlaArg: 7.024 ± 0.205
7.076AlaSer: 7.076 ± 0.168
6.067AlaThr: 6.067 ± 0.172
7.585AlaVal: 7.585 ± 0.124
1.137AlaTrp: 1.137 ± 0.044
2.808AlaTyr: 2.808 ± 0.077
0.0AlaXaa: 0.0 ± 0.0
Cys
1.641CysAla: 1.641 ± 0.067
0.281CysCys: 0.281 ± 0.027
1.102CysAsp: 1.102 ± 0.061
1.021CysGlu: 1.021 ± 0.054
0.428CysPhe: 0.428 ± 0.027
1.624CysGly: 1.624 ± 0.076
0.312CysHis: 0.312 ± 0.022
0.625CysIle: 0.625 ± 0.037
0.506CysLys: 0.506 ± 0.036
1.157CysLeu: 1.157 ± 0.055
0.329CysMet: 0.329 ± 0.025
0.378CysAsn: 0.378 ± 0.028
0.82CysPro: 0.82 ± 0.044
0.37CysGln: 0.37 ± 0.028
0.847CysArg: 0.847 ± 0.042
0.82CysSer: 0.82 ± 0.034
0.761CysThr: 0.761 ± 0.041
1.273CysVal: 1.273 ± 0.057
0.149CysTrp: 0.149 ± 0.018
0.368CysTyr: 0.368 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
7.612AspAla: 7.612 ± 0.137
0.88AspCys: 0.88 ± 0.049
4.439AspAsp: 4.439 ± 0.117
5.346AspGlu: 5.346 ± 0.143
2.494AspPhe: 2.494 ± 0.063
6.156AspGly: 6.156 ± 0.258
1.218AspHis: 1.218 ± 0.05
3.097AspIle: 3.097 ± 0.079
2.084AspLys: 2.084 ± 0.075
5.909AspLeu: 5.909 ± 0.119
1.597AspMet: 1.597 ± 0.047
1.653AspAsn: 1.653 ± 0.065
3.326AspPro: 3.326 ± 0.082
1.804AspGln: 1.804 ± 0.063
3.39AspArg: 3.39 ± 0.105
3.717AspSer: 3.717 ± 0.101
2.877AspThr: 2.877 ± 0.121
5.862AspVal: 5.862 ± 0.105
0.816AspTrp: 0.816 ± 0.046
2.153AspTyr: 2.153 ± 0.064
0.0AspXaa: 0.0 ± 0.0
Glu
6.935GluAla: 6.935 ± 0.181
0.803GluCys: 0.803 ± 0.045
3.897GluAsp: 3.897 ± 0.102
4.181GluGlu: 4.181 ± 0.145
1.933GluPhe: 1.933 ± 0.074
5.699GluGly: 5.699 ± 0.105
1.396GluHis: 1.396 ± 0.06
2.701GluIle: 2.701 ± 0.097
2.835GluLys: 2.835 ± 0.088
6.568GluLeu: 6.568 ± 0.193
1.666GluMet: 1.666 ± 0.062
1.76GluAsn: 1.76 ± 0.067
2.531GluPro: 2.531 ± 0.087
2.165GluGln: 2.165 ± 0.081
5.066GluArg: 5.066 ± 0.147
3.541GluSer: 3.541 ± 0.088
2.85GluThr: 2.85 ± 0.074
5.064GluVal: 5.064 ± 0.117
0.643GluTrp: 0.643 ± 0.033
1.581GluTyr: 1.581 ± 0.063
0.0GluXaa: 0.0 ± 0.0
Phe
3.707PheAla: 3.707 ± 0.081
0.642PheCys: 0.642 ± 0.038
2.993PheAsp: 2.993 ± 0.101
2.268PheGlu: 2.268 ± 0.06
1.394PhePhe: 1.394 ± 0.056
3.307PheGly: 3.307 ± 0.084
0.566PheHis: 0.566 ± 0.034
1.392PheIle: 1.392 ± 0.054
1.122PheLys: 1.122 ± 0.041
2.934PheLeu: 2.934 ± 0.09
0.87PheMet: 0.87 ± 0.037
1.075PheAsn: 1.075 ± 0.045
1.25PhePro: 1.25 ± 0.051
0.868PheGln: 0.868 ± 0.037
1.696PheArg: 1.696 ± 0.051
2.758PheSer: 2.758 ± 0.081
1.827PheThr: 1.827 ± 0.082
3.205PheVal: 3.205 ± 0.085
0.383PheTrp: 0.383 ± 0.028
1.088PheTyr: 1.088 ± 0.055
0.0PheXaa: 0.0 ± 0.0
Gly
8.266GlyAla: 8.266 ± 0.155
1.376GlyCys: 1.376 ± 0.051
5.298GlyAsp: 5.298 ± 0.132
5.313GlyGlu: 5.313 ± 0.128
3.265GlyPhe: 3.265 ± 0.078
6.69GlyGly: 6.69 ± 0.146
1.747GlyHis: 1.747 ± 0.065
4.802GlyIle: 4.802 ± 0.104
3.737GlyLys: 3.737 ± 0.095
7.386GlyLeu: 7.386 ± 0.124
2.375GlyMet: 2.375 ± 0.067
2.546GlyAsn: 2.546 ± 0.124
2.323GlyPro: 2.323 ± 0.054
2.394GlyGln: 2.394 ± 0.066
5.182GlyArg: 5.182 ± 0.139
6.134GlySer: 6.134 ± 0.22
5.311GlyThr: 5.311 ± 0.25
7.026GlyVal: 7.026 ± 0.129
1.251GlyTrp: 1.251 ± 0.144
2.939GlyTyr: 2.939 ± 0.14
0.0GlyXaa: 0.0 ± 0.0
His
1.992HisAla: 1.992 ± 0.067
0.292HisCys: 0.292 ± 0.025
1.424HisAsp: 1.424 ± 0.064
1.327HisGlu: 1.327 ± 0.055
0.774HisPhe: 0.774 ± 0.035
1.918HisGly: 1.918 ± 0.061
0.474HisHis: 0.474 ± 0.032
0.843HisIle: 0.843 ± 0.043
0.633HisLys: 0.633 ± 0.033
1.56HisLeu: 1.56 ± 0.055
0.465HisMet: 0.465 ± 0.027
0.543HisAsn: 0.543 ± 0.029
0.996HisPro: 0.996 ± 0.051
0.497HisGln: 0.497 ± 0.026
1.038HisArg: 1.038 ± 0.053
1.021HisSer: 1.021 ± 0.038
0.872HisThr: 0.872 ± 0.044
1.744HisVal: 1.744 ± 0.063
0.217HisTrp: 0.217 ± 0.033
0.623HisTyr: 0.623 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
5.686IleAla: 5.686 ± 0.12
0.922IleCys: 0.922 ± 0.054
3.652IleAsp: 3.652 ± 0.089
3.284IleGlu: 3.284 ± 0.1
1.418IlePhe: 1.418 ± 0.05
3.687IleGly: 3.687 ± 0.096
0.842IleHis: 0.842 ± 0.04
2.283IleIle: 2.283 ± 0.078
1.733IleLys: 1.733 ± 0.063
3.821IleLeu: 3.821 ± 0.111
1.146IleMet: 1.146 ± 0.057
1.562IleAsn: 1.562 ± 0.057
2.283IlePro: 2.283 ± 0.071
1.219IleGln: 1.219 ± 0.048
2.469IleArg: 2.469 ± 0.084
3.188IleSer: 3.188 ± 0.073
2.572IleThr: 2.572 ± 0.103
4.444IleVal: 4.444 ± 0.101
0.438IleTrp: 0.438 ± 0.039
1.273IleTyr: 1.273 ± 0.055
0.0IleXaa: 0.0 ± 0.0
Lys
4.063LysAla: 4.063 ± 0.103
0.452LysCys: 0.452 ± 0.033
2.311LysAsp: 2.311 ± 0.077
2.111LysGlu: 2.111 ± 0.092
1.043LysPhe: 1.043 ± 0.047
3.171LysGly: 3.171 ± 0.079
0.695LysHis: 0.695 ± 0.034
1.938LysIle: 1.938 ± 0.068
2.039LysLys: 2.039 ± 0.094
3.158LysLeu: 3.158 ± 0.079
0.981LysMet: 0.981 ± 0.05
1.308LysAsn: 1.308 ± 0.044
1.698LysPro: 1.698 ± 0.059
1.095LysGln: 1.095 ± 0.057
2.513LysArg: 2.513 ± 0.092
2.37LysSer: 2.37 ± 0.067
2.35LysThr: 2.35 ± 0.068
3.358LysVal: 3.358 ± 0.104
0.368LysTrp: 0.368 ± 0.03
1.021LysTyr: 1.021 ± 0.043
0.0LysXaa: 0.0 ± 0.0
Leu
10.401LeuAla: 10.401 ± 0.212
1.409LeuCys: 1.409 ± 0.062
6.347LeuAsp: 6.347 ± 0.111
5.681LeuGlu: 5.681 ± 0.155
3.087LeuPhe: 3.087 ± 0.091
7.935LeuGly: 7.935 ± 0.127
1.53LeuHis: 1.53 ± 0.055
3.699LeuIle: 3.699 ± 0.104
3.123LeuLys: 3.123 ± 0.097
7.962LeuLeu: 7.962 ± 0.213
2.345LeuMet: 2.345 ± 0.081
2.432LeuAsn: 2.432 ± 0.068
4.053LeuPro: 4.053 ± 0.088
2.133LeuGln: 2.133 ± 0.069
5.496LeuArg: 5.496 ± 0.162
6.756LeuSer: 6.756 ± 0.121
4.94LeuThr: 4.94 ± 0.153
7.8LeuVal: 7.8 ± 0.152
0.784LeuTrp: 0.784 ± 0.035
2.34LeuTyr: 2.34 ± 0.113
0.0LeuXaa: 0.0 ± 0.0
Met
2.993MetAla: 2.993 ± 0.091
0.333MetCys: 0.333 ± 0.027
1.498MetAsp: 1.498 ± 0.052
1.359MetGlu: 1.359 ± 0.055
0.793MetPhe: 0.793 ± 0.06
2.617MetGly: 2.617 ± 0.086
0.465MetHis: 0.465 ± 0.029
1.051MetIle: 1.051 ± 0.048
0.833MetLys: 0.833 ± 0.04
2.336MetLeu: 2.336 ± 0.073
0.642MetMet: 0.642 ± 0.035
0.862MetAsn: 0.862 ± 0.044
1.308MetPro: 1.308 ± 0.047
0.724MetGln: 0.724 ± 0.042
1.775MetArg: 1.775 ± 0.074
1.977MetSer: 1.977 ± 0.057
1.461MetThr: 1.461 ± 0.049
1.906MetVal: 1.906 ± 0.059
0.185MetTrp: 0.185 ± 0.019
0.543MetTyr: 0.543 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
3.159AsnAla: 3.159 ± 0.076
0.396AsnCys: 0.396 ± 0.029
1.999AsnAsp: 1.999 ± 0.088
1.698AsnGlu: 1.698 ± 0.055
0.969AsnPhe: 0.969 ± 0.041
2.617AsnGly: 2.617 ± 0.143
0.596AsnHis: 0.596 ± 0.032
1.468AsnIle: 1.468 ± 0.053
1.088AsnLys: 1.088 ± 0.046
2.706AsnLeu: 2.706 ± 0.11
0.855AsnMet: 0.855 ± 0.046
0.909AsnAsn: 0.909 ± 0.05
1.838AsnPro: 1.838 ± 0.066
0.845AsnGln: 0.845 ± 0.039
1.466AsnArg: 1.466 ± 0.055
1.587AsnSer: 1.587 ± 0.065
1.52AsnThr: 1.52 ± 0.101
2.965AsnVal: 2.965 ± 0.115
0.344AsnTrp: 0.344 ± 0.034
1.062AsnTyr: 1.062 ± 0.049
0.0AsnXaa: 0.0 ± 0.0
Pro
4.27ProAla: 4.27 ± 0.101
0.479ProCys: 0.479 ± 0.031
3.025ProAsp: 3.025 ± 0.071
3.363ProGlu: 3.363 ± 0.111
1.523ProPhe: 1.523 ± 0.053
3.267ProGly: 3.267 ± 0.064
0.826ProHis: 0.826 ± 0.038
1.871ProIle: 1.871 ± 0.061
1.702ProLys: 1.702 ± 0.062
3.374ProLeu: 3.374 ± 0.09
0.929ProMet: 0.929 ± 0.039
1.429ProAsn: 1.429 ± 0.075
0.915ProPro: 0.915 ± 0.039
1.485ProGln: 1.485 ± 0.059
2.132ProArg: 2.132 ± 0.073
2.886ProSer: 2.886 ± 0.082
2.6ProThr: 2.6 ± 0.079
3.65ProVal: 3.65 ± 0.091
0.522ProTrp: 0.522 ± 0.043
1.308ProTyr: 1.308 ± 0.049
0.0ProXaa: 0.0 ± 0.0
Gln
2.978GlnAla: 2.978 ± 0.082
0.275GlnCys: 0.275 ± 0.021
1.602GlnAsp: 1.602 ± 0.052
1.846GlnGlu: 1.846 ± 0.067
0.852GlnPhe: 0.852 ± 0.038
2.459GlnGly: 2.459 ± 0.067
0.484GlnHis: 0.484 ± 0.029
1.629GlnIle: 1.629 ± 0.053
1.386GlnLys: 1.386 ± 0.054
2.647GlnLeu: 2.647 ± 0.068
0.956GlnMet: 0.956 ± 0.039
1.04GlnAsn: 1.04 ± 0.049
1.05GlnPro: 1.05 ± 0.047
1.013GlnGln: 1.013 ± 0.049
1.875GlnArg: 1.875 ± 0.081
1.745GlnSer: 1.745 ± 0.051
1.707GlnThr: 1.707 ± 0.064
2.325GlnVal: 2.325 ± 0.071
0.356GlnTrp: 0.356 ± 0.029
0.732GlnTyr: 0.732 ± 0.039
0.0GlnXaa: 0.0 ± 0.0
Arg
6.013ArgAla: 6.013 ± 0.163
0.784ArgCys: 0.784 ± 0.044
3.803ArgAsp: 3.803 ± 0.109
4.505ArgGlu: 4.505 ± 0.149
2.163ArgPhe: 2.163 ± 0.075
4.606ArgGly: 4.606 ± 0.119
1.268ArgHis: 1.268 ± 0.055
3.126ArgIle: 3.126 ± 0.094
2.479ArgLys: 2.479 ± 0.104
5.724ArgLeu: 5.724 ± 0.161
1.754ArgMet: 1.754 ± 0.069
1.745ArgAsn: 1.745 ± 0.053
2.488ArgPro: 2.488 ± 0.079
1.898ArgGln: 1.898 ± 0.069
4.468ArgArg: 4.468 ± 0.163
3.652ArgSer: 3.652 ± 0.086
2.894ArgThr: 2.894 ± 0.063
4.715ArgVal: 4.715 ± 0.12
0.643ArgTrp: 0.643 ± 0.033
1.806ArgTyr: 1.806 ± 0.049
0.0ArgXaa: 0.0 ± 0.0
Ser
6.705SerAla: 6.705 ± 0.136
0.983SerCys: 0.983 ± 0.051
4.322SerAsp: 4.322 ± 0.1
3.694SerGlu: 3.694 ± 0.087
2.807SerPhe: 2.807 ± 0.079
6.191SerGly: 6.191 ± 0.261
1.322SerHis: 1.322 ± 0.046
3.223SerIle: 3.223 ± 0.082
2.365SerLys: 2.365 ± 0.064
5.966SerLeu: 5.966 ± 0.117
1.612SerMet: 1.612 ± 0.056
2.026SerAsn: 2.026 ± 0.141
2.446SerPro: 2.446 ± 0.06
1.987SerGln: 1.987 ± 0.057
3.83SerArg: 3.83 ± 0.082
4.765SerSer: 4.765 ± 0.185
3.621SerThr: 3.621 ± 0.188
5.351SerVal: 5.351 ± 0.144
0.897SerTrp: 0.897 ± 0.073
2.197SerTyr: 2.197 ± 0.078
0.0SerXaa: 0.0 ± 0.0
Thr
4.817ThrAla: 4.817 ± 0.152
0.845ThrCys: 0.845 ± 0.04
3.213ThrAsp: 3.213 ± 0.108
2.659ThrGlu: 2.659 ± 0.069
2.231ThrPhe: 2.231 ± 0.112
5.12ThrGly: 5.12 ± 0.221
1.171ThrHis: 1.171 ± 0.048
3.19ThrIle: 3.19 ± 0.115
1.906ThrLys: 1.906 ± 0.056
5.524ThrLeu: 5.524 ± 0.183
1.335ThrMet: 1.335 ± 0.062
1.789ThrAsn: 1.789 ± 0.117
2.919ThrPro: 2.919 ± 0.072
1.545ThrGln: 1.545 ± 0.052
2.83ThrArg: 2.83 ± 0.074
3.647ThrSer: 3.647 ± 0.224
3.289ThrThr: 3.289 ± 0.203
4.591ThrVal: 4.591 ± 0.158
0.615ThrTrp: 0.615 ± 0.061
1.816ThrTyr: 1.816 ± 0.133
0.0ThrXaa: 0.0 ± 0.0
Val
9.381ValAla: 9.381 ± 0.166
1.391ValCys: 1.391 ± 0.072
5.731ValAsp: 5.731 ± 0.111
5.314ValGlu: 5.314 ± 0.13
2.869ValPhe: 2.869 ± 0.071
6.295ValGly: 6.295 ± 0.115
1.332ValHis: 1.332 ± 0.054
4.112ValIle: 4.112 ± 0.1
2.788ValLys: 2.788 ± 0.074
7.288ValLeu: 7.288 ± 0.14
2.116ValMet: 2.116 ± 0.077
2.444ValAsn: 2.444 ± 0.063
3.752ValPro: 3.752 ± 0.084
2.032ValGln: 2.032 ± 0.062
4.943ValArg: 4.943 ± 0.122
6.195ValSer: 6.195 ± 0.134
5.261ValThr: 5.261 ± 0.22
7.577ValVal: 7.577 ± 0.166
0.894ValTrp: 0.894 ± 0.099
2.021ValTyr: 2.021 ± 0.059
0.0ValXaa: 0.0 ± 0.0
Trp
0.966TrpAla: 0.966 ± 0.042
0.19TrpCys: 0.19 ± 0.02
0.781TrpAsp: 0.781 ± 0.078
0.685TrpGlu: 0.685 ± 0.038
0.516TrpPhe: 0.516 ± 0.079
0.845TrpGly: 0.845 ± 0.044
0.255TrpHis: 0.255 ± 0.021
0.43TrpIle: 0.43 ± 0.029
0.432TrpLys: 0.432 ± 0.044
1.05TrpLeu: 1.05 ± 0.05
0.302TrpMet: 0.302 ± 0.021
0.487TrpAsn: 0.487 ± 0.065
0.403TrpPro: 0.403 ± 0.029
0.406TrpGln: 0.406 ± 0.029
0.771TrpArg: 0.771 ± 0.042
0.719TrpSer: 0.719 ± 0.064
0.608TrpThr: 0.608 ± 0.083
0.737TrpVal: 0.737 ± 0.054
0.222TrpTrp: 0.222 ± 0.048
0.358TrpTyr: 0.358 ± 0.043
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.956TyrAla: 2.956 ± 0.127
0.378TyrCys: 0.378 ± 0.027
2.251TyrAsp: 2.251 ± 0.087
1.817TyrGlu: 1.817 ± 0.053
1.082TyrPhe: 1.082 ± 0.046
2.533TyrGly: 2.533 ± 0.083
0.637TyrHis: 0.637 ± 0.035
1.013TyrIle: 1.013 ± 0.044
1.011TyrLys: 1.011 ± 0.046
2.687TyrLeu: 2.687 ± 0.078
0.559TyrMet: 0.559 ± 0.032
0.984TyrAsn: 0.984 ± 0.063
1.213TyrPro: 1.213 ± 0.05
0.915TyrGln: 0.915 ± 0.043
1.723TyrArg: 1.723 ± 0.055
1.759TyrSer: 1.759 ± 0.081
1.663TyrThr: 1.663 ± 0.155
2.558TyrVal: 2.558 ± 0.07
0.297TyrTrp: 0.297 ± 0.032
1.006TyrTyr: 1.006 ± 0.064
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1609 proteins (595354 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski