Arthrobacter phage Kitkat
Average proteome isoelectric point is 5.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140G6P7|A0A140G6P7_9CAUD Uncharacterized protein OS=Arthrobacter phage Kitkat OX=1796996 GN=71 PE=4 SV=1
MM1 pKa = 8.29 DD2 pKa = 4.67 IGGKK6 pKa = 9.6 IKK8 pKa = 9.97 IDD10 pKa = 3.35 GRR12 pKa = 11.84 EE13 pKa = 4.12 TEE15 pKa = 4.84 FLLTDD20 pKa = 3.77 EE21 pKa = 5.02 GSYY24 pKa = 9.86 IQWGGDD30 pKa = 2.89 TSTLGEE36 pKa = 4.12 RR37 pKa = 11.84 TDD39 pKa = 3.98 LLEE42 pKa = 4.57 KK43 pKa = 10.72 LGEE46 pKa = 4.53 AYY48 pKa = 10.68 VEE50 pKa = 4.03 WAQEE54 pKa = 4.12 NLCSEE59 pKa = 4.9 CKK61 pKa = 10.5 DD62 pKa = 3.21 KK63 pKa = 10.64 TLDD66 pKa = 4.41 DD67 pKa = 4.36 GEE69 pKa = 4.7 GFNGRR74 pKa = 11.84 CGNCADD80 pKa = 4.23 RR81 pKa = 11.84 AEE83 pKa = 4.62 KK84 pKa = 10.59 EE85 pKa = 4.22 GSNDD89 pKa = 4.48 DD90 pKa = 3.63 NDD92 pKa = 3.56
Molecular weight: 10.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.244
IPC2_protein 4.037
IPC_protein 3.986
Toseland 3.795
ProMoST 4.075
Dawson 3.961
Bjellqvist 4.151
Wikipedia 3.872
Rodwell 3.821
Grimsley 3.706
Solomon 3.948
Lehninger 3.91
Nozaki 4.075
DTASelect 4.253
Thurlkill 3.834
EMBOSS 3.872
Sillero 4.101
Patrickios 3.592
IPC_peptide 3.948
IPC2_peptide 4.088
IPC2.peptide.svr19 4.032
Protein with the highest isoelectric point:
>tr|A0A140G6N5|A0A140G6N5_9CAUD Uncharacterized protein OS=Arthrobacter phage Kitkat OX=1796996 GN=59 PE=4 SV=1
MM1 pKa = 7.37 RR2 pKa = 11.84 RR3 pKa = 11.84 VGISLVILAGVIFVVCMYY21 pKa = 10.19 LFIMAIWEE29 pKa = 4.22 GDD31 pKa = 3.27 GRR33 pKa = 11.84 LGWIGGLLTISFLISIIAGGAMIDD57 pKa = 3.78 HH58 pKa = 7.18 ASKK61 pKa = 10.76 RR62 pKa = 11.84 IPRR65 pKa = 3.88
Molecular weight: 7.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.277
IPC2_protein 9.282
IPC_protein 10.028
Toseland 10.028
ProMoST 10.73
Dawson 10.248
Bjellqvist 10.028
Wikipedia 10.467
Rodwell 10.321
Grimsley 10.321
Solomon 10.394
Lehninger 10.379
Nozaki 10.131
DTASelect 9.999
Thurlkill 10.101
EMBOSS 10.438
Sillero 10.189
Patrickios 10.423
IPC_peptide 10.394
IPC2_peptide 9.355
IPC2.peptide.svr19 8.551
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
100
0
100
18378
39
1704
183.8
20.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.898 ± 0.306
0.881 ± 0.129
6.676 ± 0.335
6.829 ± 0.488
3.102 ± 0.218
8.053 ± 0.453
2.111 ± 0.216
4.865 ± 0.248
4.984 ± 0.274
7.879 ± 0.296
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.623 ± 0.131
3.651 ± 0.232
5.028 ± 0.245
3.749 ± 0.219
5.556 ± 0.286
5.458 ± 0.334
6.426 ± 0.396
7.248 ± 0.285
2.449 ± 0.208
2.536 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here