Amino acid dipepetide frequency for Arthrobacter phage Kitkat

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.135AlaAla: 12.135 ± 0.894
0.871AlaCys: 0.871 ± 0.21
5.822AlaAsp: 5.822 ± 0.53
6.203AlaGlu: 6.203 ± 0.599
2.068AlaPhe: 2.068 ± 0.329
6.584AlaGly: 6.584 ± 0.845
2.122AlaHis: 2.122 ± 0.392
4.897AlaIle: 4.897 ± 0.763
5.714AlaLys: 5.714 ± 0.732
9.033AlaLeu: 9.033 ± 0.88
2.993AlaMet: 2.993 ± 0.382
3.428AlaAsn: 3.428 ± 0.623
4.843AlaPro: 4.843 ± 0.579
3.7AlaGln: 3.7 ± 0.519
4.734AlaArg: 4.734 ± 0.478
4.571AlaSer: 4.571 ± 0.465
6.149AlaThr: 6.149 ± 0.599
8.543AlaVal: 8.543 ± 0.69
1.959AlaTrp: 1.959 ± 0.312
2.612AlaTyr: 2.612 ± 0.405
0.0AlaXaa: 0.0 ± 0.0
Cys
0.599CysAla: 0.599 ± 0.132
0.272CysCys: 0.272 ± 0.124
0.435CysAsp: 0.435 ± 0.21
0.925CysGlu: 0.925 ± 0.256
0.163CysPhe: 0.163 ± 0.104
0.599CysGly: 0.599 ± 0.184
0.49CysHis: 0.49 ± 0.161
0.435CysIle: 0.435 ± 0.173
0.109CysLys: 0.109 ± 0.07
0.653CysLeu: 0.653 ± 0.185
0.218CysMet: 0.218 ± 0.101
0.326CysAsn: 0.326 ± 0.134
0.653CysPro: 0.653 ± 0.165
0.435CysGln: 0.435 ± 0.158
0.599CysArg: 0.599 ± 0.179
0.544CysSer: 0.544 ± 0.159
0.49CysThr: 0.49 ± 0.154
0.49CysVal: 0.49 ± 0.179
0.163CysTrp: 0.163 ± 0.088
0.218CysTyr: 0.218 ± 0.106
0.0CysXaa: 0.0 ± 0.0
Asp
5.822AspAla: 5.822 ± 0.525
0.871AspCys: 0.871 ± 0.211
4.462AspAsp: 4.462 ± 0.876
4.68AspGlu: 4.68 ± 0.614
2.503AspPhe: 2.503 ± 0.382
7.346AspGly: 7.346 ± 0.853
1.034AspHis: 1.034 ± 0.323
2.34AspIle: 2.34 ± 0.29
2.449AspLys: 2.449 ± 0.336
6.965AspLeu: 6.965 ± 0.616
2.34AspMet: 2.34 ± 0.34
1.741AspAsn: 1.741 ± 0.322
3.537AspPro: 3.537 ± 0.419
2.177AspGln: 2.177 ± 0.355
2.884AspArg: 2.884 ± 0.446
3.428AspSer: 3.428 ± 0.512
3.265AspThr: 3.265 ± 0.426
5.224AspVal: 5.224 ± 0.578
2.013AspTrp: 2.013 ± 0.339
1.687AspTyr: 1.687 ± 0.314
0.0AspXaa: 0.0 ± 0.0
Glu
6.367GluAla: 6.367 ± 0.631
0.544GluCys: 0.544 ± 0.197
4.244GluAsp: 4.244 ± 0.592
5.496GluGlu: 5.496 ± 0.747
2.177GluPhe: 2.177 ± 0.346
4.897GluGly: 4.897 ± 0.521
1.905GluHis: 1.905 ± 0.383
4.571GluIle: 4.571 ± 0.578
3.319GluLys: 3.319 ± 0.55
6.312GluLeu: 6.312 ± 0.747
1.306GluMet: 1.306 ± 0.255
2.285GluAsn: 2.285 ± 0.34
2.503GluPro: 2.503 ± 0.427
3.428GluGln: 3.428 ± 0.468
3.864GluArg: 3.864 ± 0.565
2.83GluSer: 2.83 ± 0.338
3.755GluThr: 3.755 ± 0.47
4.897GluVal: 4.897 ± 0.608
1.578GluTrp: 1.578 ± 0.271
2.013GluTyr: 2.013 ± 0.377
0.0GluXaa: 0.0 ± 0.0
Phe
2.83PheAla: 2.83 ± 0.419
0.0PheCys: 0.0 ± 0.0
1.796PheAsp: 1.796 ± 0.323
2.013PheGlu: 2.013 ± 0.312
0.871PhePhe: 0.871 ± 0.221
2.666PheGly: 2.666 ± 0.456
0.762PheHis: 0.762 ± 0.231
1.524PheIle: 1.524 ± 0.446
1.088PheLys: 1.088 ± 0.257
2.775PheLeu: 2.775 ± 0.302
0.707PheMet: 0.707 ± 0.195
1.632PheAsn: 1.632 ± 0.281
1.252PhePro: 1.252 ± 0.274
0.871PheGln: 0.871 ± 0.197
1.905PheArg: 1.905 ± 0.274
1.85PheSer: 1.85 ± 0.347
2.775PheThr: 2.775 ± 0.457
2.285PheVal: 2.285 ± 0.418
0.925PheTrp: 0.925 ± 0.203
0.49PheTyr: 0.49 ± 0.134
0.0PheXaa: 0.0 ± 0.0
Gly
7.673GlyAla: 7.673 ± 1.196
0.49GlyCys: 0.49 ± 0.169
4.517GlyAsp: 4.517 ± 0.426
4.408GlyGlu: 4.408 ± 0.59
3.265GlyPhe: 3.265 ± 0.668
5.931GlyGly: 5.931 ± 0.678
1.415GlyHis: 1.415 ± 0.284
3.972GlyIle: 3.972 ± 0.495
5.659GlyLys: 5.659 ± 0.558
5.768GlyLeu: 5.768 ± 0.587
2.231GlyMet: 2.231 ± 0.402
3.374GlyAsn: 3.374 ± 0.457
3.102GlyPro: 3.102 ± 0.46
2.938GlyGln: 2.938 ± 0.402
4.517GlyArg: 4.517 ± 0.507
5.278GlySer: 5.278 ± 0.873
5.006GlyThr: 5.006 ± 0.857
6.965GlyVal: 6.965 ± 0.577
1.687GlyTrp: 1.687 ± 0.303
2.34GlyTyr: 2.34 ± 0.391
0.0GlyXaa: 0.0 ± 0.0
His
1.741HisAla: 1.741 ± 0.303
0.272HisCys: 0.272 ± 0.131
1.415HisAsp: 1.415 ± 0.298
1.578HisGlu: 1.578 ± 0.345
0.544HisPhe: 0.544 ± 0.159
1.741HisGly: 1.741 ± 0.319
0.599HisHis: 0.599 ± 0.216
0.925HisIle: 0.925 ± 0.253
0.816HisLys: 0.816 ± 0.169
1.796HisLeu: 1.796 ± 0.317
0.49HisMet: 0.49 ± 0.169
0.381HisAsn: 0.381 ± 0.144
1.143HisPro: 1.143 ± 0.322
0.544HisGln: 0.544 ± 0.172
1.306HisArg: 1.306 ± 0.261
0.925HisSer: 0.925 ± 0.258
1.143HisThr: 1.143 ± 0.227
2.449HisVal: 2.449 ± 0.361
0.762HisTrp: 0.762 ± 0.225
0.544HisTyr: 0.544 ± 0.164
0.0HisXaa: 0.0 ± 0.0
Ile
4.843IleAla: 4.843 ± 0.378
0.49IleCys: 0.49 ± 0.146
3.646IleAsp: 3.646 ± 0.447
3.918IleGlu: 3.918 ± 0.584
1.524IlePhe: 1.524 ± 0.373
3.918IleGly: 3.918 ± 0.566
0.653IleHis: 0.653 ± 0.217
2.285IleIle: 2.285 ± 0.34
2.068IleLys: 2.068 ± 0.305
3.7IleLeu: 3.7 ± 0.47
1.088IleMet: 1.088 ± 0.389
1.959IleAsn: 1.959 ± 0.341
2.122IlePro: 2.122 ± 0.391
2.231IleGln: 2.231 ± 0.307
2.775IleArg: 2.775 ± 0.299
2.775IleSer: 2.775 ± 0.356
3.374IleThr: 3.374 ± 0.409
2.721IleVal: 2.721 ± 0.457
1.36IleTrp: 1.36 ± 0.52
1.197IleTyr: 1.197 ± 0.261
0.0IleXaa: 0.0 ± 0.0
Lys
5.333LysAla: 5.333 ± 0.585
0.109LysCys: 0.109 ± 0.073
3.7LysAsp: 3.7 ± 0.502
3.918LysGlu: 3.918 ± 0.552
1.578LysPhe: 1.578 ± 0.309
2.938LysGly: 2.938 ± 0.43
1.36LysHis: 1.36 ± 0.283
2.449LysIle: 2.449 ± 0.408
3.047LysLys: 3.047 ± 0.359
4.081LysLeu: 4.081 ± 0.458
1.524LysMet: 1.524 ± 0.272
1.687LysAsn: 1.687 ± 0.287
2.993LysPro: 2.993 ± 0.477
2.231LysGln: 2.231 ± 0.42
2.612LysArg: 2.612 ± 0.407
2.231LysSer: 2.231 ± 0.307
2.449LysThr: 2.449 ± 0.316
3.374LysVal: 3.374 ± 0.41
0.707LysTrp: 0.707 ± 0.202
1.524LysTyr: 1.524 ± 0.299
0.0LysXaa: 0.0 ± 0.0
Leu
7.781LeuAla: 7.781 ± 0.525
0.599LeuCys: 0.599 ± 0.163
5.768LeuAsp: 5.768 ± 0.635
4.734LeuGlu: 4.734 ± 0.505
1.469LeuPhe: 1.469 ± 0.249
5.931LeuGly: 5.931 ± 0.855
2.177LeuHis: 2.177 ± 0.329
3.755LeuIle: 3.755 ± 0.316
3.047LeuLys: 3.047 ± 0.435
6.53LeuLeu: 6.53 ± 0.607
2.013LeuMet: 2.013 ± 0.411
3.374LeuAsn: 3.374 ± 0.393
4.897LeuPro: 4.897 ± 0.602
2.938LeuGln: 2.938 ± 0.43
4.517LeuArg: 4.517 ± 0.45
4.136LeuSer: 4.136 ± 0.436
5.986LeuThr: 5.986 ± 0.536
5.333LeuVal: 5.333 ± 0.527
2.013LeuTrp: 2.013 ± 0.306
1.796LeuTyr: 1.796 ± 0.3
0.0LeuXaa: 0.0 ± 0.0
Met
3.864MetAla: 3.864 ± 0.421
0.163MetCys: 0.163 ± 0.103
1.796MetAsp: 1.796 ± 0.259
2.177MetGlu: 2.177 ± 0.334
0.816MetPhe: 0.816 ± 0.229
1.687MetGly: 1.687 ± 0.246
0.707MetHis: 0.707 ± 0.168
1.088MetIle: 1.088 ± 0.246
1.143MetLys: 1.143 ± 0.23
1.524MetLeu: 1.524 ± 0.29
0.653MetMet: 0.653 ± 0.212
1.034MetAsn: 1.034 ± 0.293
0.979MetPro: 0.979 ± 0.209
0.816MetGln: 0.816 ± 0.244
1.524MetArg: 1.524 ± 0.295
1.524MetSer: 1.524 ± 0.264
2.231MetThr: 2.231 ± 0.35
1.578MetVal: 1.578 ± 0.303
0.544MetTrp: 0.544 ± 0.173
0.381MetTyr: 0.381 ± 0.129
0.0MetXaa: 0.0 ± 0.0
Asn
3.809AsnAla: 3.809 ± 0.473
0.109AsnCys: 0.109 ± 0.07
2.122AsnAsp: 2.122 ± 0.34
2.068AsnGlu: 2.068 ± 0.295
1.034AsnPhe: 1.034 ± 0.263
4.299AsnGly: 4.299 ± 0.645
0.762AsnHis: 0.762 ± 0.144
1.524AsnIle: 1.524 ± 0.249
1.306AsnLys: 1.306 ± 0.287
2.177AsnLeu: 2.177 ± 0.396
1.143AsnMet: 1.143 ± 0.223
1.197AsnAsn: 1.197 ± 0.339
2.285AsnPro: 2.285 ± 0.429
1.524AsnGln: 1.524 ± 0.249
1.796AsnArg: 1.796 ± 0.357
2.884AsnSer: 2.884 ± 0.473
1.524AsnThr: 1.524 ± 0.302
2.666AsnVal: 2.666 ± 0.517
1.306AsnTrp: 1.306 ± 0.281
0.925AsnTyr: 0.925 ± 0.18
0.0AsnXaa: 0.0 ± 0.0
Pro
5.006ProAla: 5.006 ± 0.43
0.326ProCys: 0.326 ± 0.123
4.244ProAsp: 4.244 ± 0.504
4.299ProGlu: 4.299 ± 0.571
1.578ProPhe: 1.578 ± 0.286
4.353ProGly: 4.353 ± 0.748
0.762ProHis: 0.762 ± 0.212
1.687ProIle: 1.687 ± 0.351
2.068ProLys: 2.068 ± 0.397
3.428ProLeu: 3.428 ± 0.44
1.034ProMet: 1.034 ± 0.22
1.524ProAsn: 1.524 ± 0.306
2.884ProPro: 2.884 ± 0.48
1.687ProGln: 1.687 ± 0.264
2.884ProArg: 2.884 ± 0.386
2.666ProSer: 2.666 ± 0.425
3.319ProThr: 3.319 ± 0.375
4.136ProVal: 4.136 ± 0.507
1.088ProTrp: 1.088 ± 0.245
1.306ProTyr: 1.306 ± 0.262
0.0ProXaa: 0.0 ± 0.0
Gln
4.027GlnAla: 4.027 ± 0.414
0.381GlnCys: 0.381 ± 0.172
1.85GlnAsp: 1.85 ± 0.345
2.612GlnGlu: 2.612 ± 0.393
1.524GlnPhe: 1.524 ± 0.293
2.449GlnGly: 2.449 ± 0.326
0.925GlnHis: 0.925 ± 0.238
2.231GlnIle: 2.231 ± 0.394
1.905GlnLys: 1.905 ± 0.375
3.755GlnLeu: 3.755 ± 0.488
1.088GlnMet: 1.088 ± 0.226
0.979GlnAsn: 0.979 ± 0.187
1.959GlnPro: 1.959 ± 0.319
1.85GlnGln: 1.85 ± 0.372
2.231GlnArg: 2.231 ± 0.391
1.85GlnSer: 1.85 ± 0.314
1.796GlnThr: 1.796 ± 0.365
2.231GlnVal: 2.231 ± 0.29
0.762GlnTrp: 0.762 ± 0.189
1.088GlnTyr: 1.088 ± 0.292
0.0GlnXaa: 0.0 ± 0.0
Arg
4.408ArgAla: 4.408 ± 0.446
0.653ArgCys: 0.653 ± 0.175
3.646ArgAsp: 3.646 ± 0.408
4.299ArgGlu: 4.299 ± 0.561
2.231ArgPhe: 2.231 ± 0.375
3.864ArgGly: 3.864 ± 0.432
1.252ArgHis: 1.252 ± 0.256
3.211ArgIle: 3.211 ± 0.403
4.462ArgLys: 4.462 ± 0.573
3.374ArgLeu: 3.374 ± 0.396
2.122ArgMet: 2.122 ± 0.435
1.796ArgAsn: 1.796 ± 0.363
2.231ArgPro: 2.231 ± 0.284
2.122ArgGln: 2.122 ± 0.4
3.918ArgArg: 3.918 ± 0.532
2.177ArgSer: 2.177 ± 0.305
3.972ArgThr: 3.972 ± 0.441
2.83ArgVal: 2.83 ± 0.352
1.36ArgTrp: 1.36 ± 0.276
1.632ArgTyr: 1.632 ± 0.317
0.0ArgXaa: 0.0 ± 0.0
Ser
4.462SerAla: 4.462 ± 0.473
0.599SerCys: 0.599 ± 0.183
3.156SerAsp: 3.156 ± 0.463
3.374SerGlu: 3.374 ± 0.361
2.122SerPhe: 2.122 ± 0.311
6.095SerGly: 6.095 ± 0.794
0.925SerHis: 0.925 ± 0.211
2.938SerIle: 2.938 ± 0.531
2.612SerLys: 2.612 ± 0.295
3.591SerLeu: 3.591 ± 0.452
1.578SerMet: 1.578 ± 0.273
2.122SerAsn: 2.122 ± 0.339
2.503SerPro: 2.503 ± 0.354
2.013SerGln: 2.013 ± 0.277
2.612SerArg: 2.612 ± 0.4
3.265SerSer: 3.265 ± 0.457
4.081SerThr: 4.081 ± 0.539
4.081SerVal: 4.081 ± 0.568
1.415SerTrp: 1.415 ± 0.278
1.034SerTyr: 1.034 ± 0.222
0.0SerXaa: 0.0 ± 0.0
Thr
7.618ThrAla: 7.618 ± 0.904
0.544ThrCys: 0.544 ± 0.176
4.353ThrAsp: 4.353 ± 0.575
4.462ThrGlu: 4.462 ± 0.455
2.013ThrPhe: 2.013 ± 0.354
5.605ThrGly: 5.605 ± 0.798
1.034ThrHis: 1.034 ± 0.222
3.047ThrIle: 3.047 ± 0.395
2.394ThrLys: 2.394 ± 0.353
5.115ThrLeu: 5.115 ± 0.512
0.871ThrMet: 0.871 ± 0.22
2.449ThrAsn: 2.449 ± 0.424
3.918ThrPro: 3.918 ± 0.458
1.959ThrGln: 1.959 ± 0.371
2.775ThrArg: 2.775 ± 0.351
3.646ThrSer: 3.646 ± 0.754
5.006ThrThr: 5.006 ± 0.562
4.68ThrVal: 4.68 ± 0.441
0.979ThrTrp: 0.979 ± 0.258
1.796ThrTyr: 1.796 ± 0.297
0.0ThrXaa: 0.0 ± 0.0
Val
6.584ValAla: 6.584 ± 0.67
0.925ValCys: 0.925 ± 0.226
6.367ValAsp: 6.367 ± 0.535
3.864ValGlu: 3.864 ± 0.539
2.068ValPhe: 2.068 ± 0.385
5.333ValGly: 5.333 ± 0.571
1.143ValHis: 1.143 ± 0.279
3.537ValIle: 3.537 ± 0.443
4.571ValLys: 4.571 ± 0.563
5.224ValLeu: 5.224 ± 0.502
1.632ValMet: 1.632 ± 0.296
2.721ValAsn: 2.721 ± 0.467
3.755ValPro: 3.755 ± 0.447
2.884ValGln: 2.884 ± 0.305
5.061ValArg: 5.061 ± 0.454
3.7ValSer: 3.7 ± 0.541
4.789ValThr: 4.789 ± 0.681
4.625ValVal: 4.625 ± 0.454
2.122ValTrp: 2.122 ± 0.509
1.578ValTyr: 1.578 ± 0.288
0.0ValXaa: 0.0 ± 0.0
Trp
1.85TrpAla: 1.85 ± 0.283
0.381TrpCys: 0.381 ± 0.131
1.632TrpAsp: 1.632 ± 0.292
1.85TrpGlu: 1.85 ± 0.314
0.707TrpPhe: 0.707 ± 0.185
1.632TrpGly: 1.632 ± 0.341
0.707TrpHis: 0.707 ± 0.206
1.36TrpIle: 1.36 ± 0.299
1.034TrpLys: 1.034 ± 0.26
1.469TrpLeu: 1.469 ± 0.259
0.544TrpMet: 0.544 ± 0.158
1.632TrpAsn: 1.632 ± 0.574
1.034TrpPro: 1.034 ± 0.244
0.653TrpGln: 0.653 ± 0.162
0.871TrpArg: 0.871 ± 0.214
1.905TrpSer: 1.905 ± 0.413
1.905TrpThr: 1.905 ± 0.31
1.741TrpVal: 1.741 ± 0.283
1.034TrpTrp: 1.034 ± 0.286
0.544TrpTyr: 0.544 ± 0.148
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.231TyrAla: 2.231 ± 0.356
0.218TyrCys: 0.218 ± 0.107
1.796TyrAsp: 1.796 ± 0.262
1.415TyrGlu: 1.415 ± 0.288
0.762TyrPhe: 0.762 ± 0.174
2.666TyrGly: 2.666 ± 0.349
0.381TyrHis: 0.381 ± 0.149
0.871TyrIle: 0.871 ± 0.194
1.034TyrLys: 1.034 ± 0.279
1.524TyrLeu: 1.524 ± 0.277
0.599TyrMet: 0.599 ± 0.186
0.871TyrAsn: 0.871 ± 0.205
1.687TyrPro: 1.687 ± 0.36
0.49TyrGln: 0.49 ± 0.161
2.177TyrArg: 2.177 ± 0.353
2.394TyrSer: 2.394 ± 0.389
1.252TyrThr: 1.252 ± 0.259
1.632TyrVal: 1.632 ± 0.317
0.707TyrTrp: 0.707 ± 0.16
0.653TyrTyr: 0.653 ± 0.169
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 100 proteins (18378 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski