Saliterribacillus persicus
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3585 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A368YCH0|A0A368YCH0_9BACI Uncharacterized protein OS=Saliterribacillus persicus OX=930114 GN=DFR57_101241 PE=4 SV=1
MM1 pKa = 7.65 PKK3 pKa = 10.44 VIILFCSMSGNTEE16 pKa = 3.93 EE17 pKa = 5.07 IADD20 pKa = 3.58 IMEE23 pKa = 4.4 TNITNHH29 pKa = 6.11 GLDD32 pKa = 3.45 VEE34 pKa = 4.84 KK35 pKa = 10.7 IQIDD39 pKa = 3.53 MDD41 pKa = 4.13 DD42 pKa = 4.28 FLITDD47 pKa = 4.73 LLNYY51 pKa = 9.39 EE52 pKa = 5.03 AILFGTYY59 pKa = 7.24 TWGDD63 pKa = 2.66 GDD65 pKa = 3.98 IPYY68 pKa = 10.09 EE69 pKa = 4.69 LEE71 pKa = 4.7 DD72 pKa = 5.14 FYY74 pKa = 11.66 DD75 pKa = 5.24 DD76 pKa = 3.92 MDD78 pKa = 5.38 EE79 pKa = 4.55 IDD81 pKa = 3.65 LTGKK85 pKa = 10.04 VVGLFGSCDD94 pKa = 3.18 SMYY97 pKa = 10.22 PDD99 pKa = 3.7 YY100 pKa = 11.38 GGAIDD105 pKa = 4.0 TFAEE109 pKa = 4.21 RR110 pKa = 11.84 FKK112 pKa = 11.32 EE113 pKa = 3.97 RR114 pKa = 11.84 GASNVLYY121 pKa = 10.13 NLKK124 pKa = 10.07 IEE126 pKa = 4.77 LSPDD130 pKa = 3.02 QDD132 pKa = 4.23 DD133 pKa = 4.36 VEE135 pKa = 4.55 RR136 pKa = 11.84 CQKK139 pKa = 10.2 FAEE142 pKa = 4.47 DD143 pKa = 4.05 FVKK146 pKa = 10.41 EE147 pKa = 3.61 LHH149 pKa = 6.89 AYY151 pKa = 10.18 AEE153 pKa = 4.36
Molecular weight: 17.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.808
IPC_protein 3.795
Toseland 3.579
ProMoST 3.935
Dawson 3.783
Bjellqvist 3.973
Wikipedia 3.706
Rodwell 3.63
Grimsley 3.49
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.126
Thurlkill 3.63
EMBOSS 3.719
Sillero 3.923
Patrickios 1.875
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.829
Protein with the highest isoelectric point:
>tr|A0A368XF62|A0A368XF62_9BACI Sulfite reductase [NADPH] hemoprotein beta-component OS=Saliterribacillus persicus OX=930114 GN=cysI PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 KK11 pKa = 9.23 RR12 pKa = 11.84 KK13 pKa = 8.22 KK14 pKa = 8.69 VHH16 pKa = 5.46 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTKK25 pKa = 10.08 NGRR28 pKa = 11.84 NVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 KK36 pKa = 9.36 KK37 pKa = 10.05 GRR39 pKa = 11.84 KK40 pKa = 8.66 VLSAA44 pKa = 4.05
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.735
Grimsley 13.013
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.457
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.137
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3585
0
3585
1071052
29
2398
298.8
33.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.579 ± 0.036
0.62 ± 0.012
5.502 ± 0.03
7.902 ± 0.052
4.69 ± 0.036
6.367 ± 0.035
2.066 ± 0.02
8.363 ± 0.041
7.023 ± 0.039
9.682 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.716 ± 0.02
4.898 ± 0.034
3.392 ± 0.024
3.653 ± 0.023
3.675 ± 0.028
6.0 ± 0.032
5.404 ± 0.025
6.676 ± 0.028
1.084 ± 0.018
3.707 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here