Arenibacter aquaticus
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3975 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S0D2L4|A0A3S0D2L4_9FLAO YjgP/YjgQ family permease OS=Arenibacter aquaticus OX=2489054 GN=EHW67_20215 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 9.52 GTVYY6 pKa = 10.2 RR7 pKa = 11.84 FKK9 pKa = 10.33 MLTMASASLCLMASCSSDD27 pKa = 4.51 DD28 pKa = 5.6 DD29 pKa = 5.41 DD30 pKa = 7.2 DD31 pKa = 5.54 EE32 pKa = 6.05 LGNWTDD38 pKa = 3.24 KK39 pKa = 11.3 SVFDD43 pKa = 3.93 GSPRR47 pKa = 11.84 SSSAYY52 pKa = 8.55 FTIGNIGYY60 pKa = 9.93 SGVGYY65 pKa = 10.75 DD66 pKa = 3.83 GDD68 pKa = 5.23 DD69 pKa = 3.56 YY70 pKa = 11.37 LTSFWAYY77 pKa = 11.09 DD78 pKa = 3.24 MDD80 pKa = 5.17 GDD82 pKa = 4.08 YY83 pKa = 10.58 WSQKK87 pKa = 10.74 ADD89 pKa = 3.71 FPGAARR95 pKa = 11.84 NAAVGFAIDD104 pKa = 3.44 NKK106 pKa = 10.56 GYY108 pKa = 10.21 IGSGYY113 pKa = 10.56 DD114 pKa = 3.77 GLDD117 pKa = 3.51 EE118 pKa = 5.9 LSDD121 pKa = 4.12 FYY123 pKa = 11.7 SYY125 pKa = 11.4 DD126 pKa = 3.39 PSSNTWTAIAEE137 pKa = 4.45 FPSTPRR143 pKa = 11.84 RR144 pKa = 11.84 SALAFGINGYY154 pKa = 10.59 GYY156 pKa = 10.45 FGTGFDD162 pKa = 4.34 GDD164 pKa = 3.78 NDD166 pKa = 3.98 RR167 pKa = 11.84 KK168 pKa = 10.91 DD169 pKa = 3.13 FWKK172 pKa = 10.74 YY173 pKa = 11.28 DD174 pKa = 3.77 PNTDD178 pKa = 2.85 SWSEE182 pKa = 3.73 LVGFGGDD189 pKa = 3.25 KK190 pKa = 10.27 RR191 pKa = 11.84 RR192 pKa = 11.84 SATTFTIGDD201 pKa = 3.93 KK202 pKa = 10.92 VYY204 pKa = 10.26 MGTGVSNGIYY214 pKa = 10.36 LDD216 pKa = 4.25 DD217 pKa = 3.93 FWVFDD222 pKa = 4.03 SATEE226 pKa = 3.81 SWTQLLDD233 pKa = 3.75 LDD235 pKa = 4.83 EE236 pKa = 5.2 EE237 pKa = 4.87 DD238 pKa = 4.87 DD239 pKa = 4.03 YY240 pKa = 12.08 SIVRR244 pKa = 11.84 SNAVGFTLNDD254 pKa = 3.15 YY255 pKa = 11.12 GYY257 pKa = 9.93 IATGTSGGTLQSVWEE272 pKa = 4.25 YY273 pKa = 11.53 DD274 pKa = 3.51 PSNDD278 pKa = 2.54 TWEE281 pKa = 4.45 AKK283 pKa = 9.7 TSFEE287 pKa = 3.95 RR288 pKa = 11.84 TSRR291 pKa = 11.84 QDD293 pKa = 3.15 AVVFSNNVRR302 pKa = 11.84 AFIGLGRR309 pKa = 11.84 TGSLYY314 pKa = 10.87 LDD316 pKa = 4.28 DD317 pKa = 5.7 LEE319 pKa = 5.55 EE320 pKa = 4.09 FFPYY324 pKa = 10.34 DD325 pKa = 3.6 EE326 pKa = 5.37 YY327 pKa = 11.74 DD328 pKa = 4.78 DD329 pKa = 4.65 EE330 pKa = 5.01 DD331 pKa = 3.64
Molecular weight: 36.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.673
IPC2_protein 3.757
IPC_protein 3.808
Toseland 3.567
ProMoST 3.961
Dawson 3.808
Bjellqvist 3.986
Wikipedia 3.77
Rodwell 3.617
Grimsley 3.465
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.215
Thurlkill 3.63
EMBOSS 3.783
Sillero 3.923
Patrickios 1.341
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.834
Protein with the highest isoelectric point:
>tr|A0A3S0BVA2|A0A3S0BVA2_9FLAO Response regulator OS=Arenibacter aquaticus OX=2489054 GN=EHW67_19735 PE=4 SV=1
MM1 pKa = 7.79 RR2 pKa = 11.84 SFRR5 pKa = 11.84 FIDD8 pKa = 3.26 QEE10 pKa = 3.98 FRR12 pKa = 11.84 DD13 pKa = 3.94 VSTLLRR19 pKa = 11.84 RR20 pKa = 11.84 YY21 pKa = 8.11 TKK23 pKa = 9.89 IHH25 pKa = 5.95 FKK27 pKa = 10.2 KK28 pKa = 10.42 DD29 pKa = 3.35 YY30 pKa = 8.87 MLLRR34 pKa = 11.84 SIPSIGPIVASGILSEE50 pKa = 5.23 LGDD53 pKa = 3.65 LRR55 pKa = 11.84 RR56 pKa = 11.84 FKK58 pKa = 10.69 NIKK61 pKa = 9.53 HH62 pKa = 5.54 LAGYY66 pKa = 9.8 VGLAPGIYY74 pKa = 9.76 QSGDD78 pKa = 3.37 TVRR81 pKa = 11.84 HH82 pKa = 5.63 TGVSMRR88 pKa = 11.84 AHH90 pKa = 6.49 RR91 pKa = 11.84 LIRR94 pKa = 11.84 SYY96 pKa = 10.66 FIEE99 pKa = 4.74 ASWQAIRR106 pKa = 11.84 TDD108 pKa = 3.41 PVMQAFYY115 pKa = 10.63 RR116 pKa = 11.84 KK117 pKa = 8.99 HH118 pKa = 5.74 HH119 pKa = 5.88 GKK121 pKa = 9.2 NVKK124 pKa = 10.36 SIIVKK129 pKa = 8.57 VARR132 pKa = 11.84 KK133 pKa = 9.33 LLSRR137 pKa = 11.84 TLAVIKK143 pKa = 10.07 TEE145 pKa = 3.95 TPYY148 pKa = 11.19 VIGVLEE154 pKa = 3.97
Molecular weight: 17.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.313
IPC2_protein 9.955
IPC_protein 10.745
Toseland 10.789
ProMoST 10.482
Dawson 10.891
Bjellqvist 10.599
Wikipedia 11.111
Rodwell 11.169
Grimsley 10.95
Solomon 10.994
Lehninger 10.965
Nozaki 10.745
DTASelect 10.599
Thurlkill 10.789
EMBOSS 11.184
Sillero 10.818
Patrickios 10.877
IPC_peptide 10.994
IPC2_peptide 9.414
IPC2.peptide.svr19 8.402
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3975
0
3975
1516357
29
2814
381.5
42.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.539 ± 0.034
0.725 ± 0.012
5.741 ± 0.035
6.528 ± 0.036
4.84 ± 0.025
7.143 ± 0.054
1.878 ± 0.018
7.213 ± 0.035
7.306 ± 0.042
9.371 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.333 ± 0.015
5.867 ± 0.035
3.759 ± 0.022
3.416 ± 0.022
3.667 ± 0.023
6.476 ± 0.028
5.587 ± 0.04
6.234 ± 0.029
1.251 ± 0.017
4.127 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here