Mariprofundus erugo
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2574 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5R9H0F2|A0A5R9H0F2_9PROT HDOD domain-containing protein OS=Mariprofundus erugo OX=2528639 GN=FEF65_02090 PE=4 SV=1
MM1 pKa = 7.41 DD2 pKa = 4.09 TEE4 pKa = 4.46 TFRR7 pKa = 11.84 MLAEE11 pKa = 3.82 QAFNEE16 pKa = 4.14 LPEE19 pKa = 4.15 PFRR22 pKa = 11.84 LVMEE26 pKa = 4.2 NVVIVVDD33 pKa = 5.02 DD34 pKa = 3.93 YY35 pKa = 12.37 ADD37 pKa = 4.05 AEE39 pKa = 4.6 VLASMGLTSPLDD51 pKa = 3.56 LLGLYY56 pKa = 9.18 EE57 pKa = 3.97 GHH59 pKa = 7.02 PLEE62 pKa = 4.69 EE63 pKa = 4.38 RR64 pKa = 11.84 TDD66 pKa = 3.6 GGAGMLPDD74 pKa = 4.89 MIHH77 pKa = 7.32 LYY79 pKa = 9.9 RR80 pKa = 11.84 VPILASCHH88 pKa = 4.42 EE89 pKa = 4.53 TGEE92 pKa = 4.29 EE93 pKa = 3.83 PADD96 pKa = 4.3 CIAAVVMHH104 pKa = 6.29 EE105 pKa = 4.03 VGHH108 pKa = 5.62 YY109 pKa = 10.36 FGFSDD114 pKa = 4.89 EE115 pKa = 4.26 EE116 pKa = 4.1 MEE118 pKa = 4.83 EE119 pKa = 3.83 IDD121 pKa = 5.35 IAGEE125 pKa = 3.92 DD126 pKa = 3.77 DD127 pKa = 3.36
Molecular weight: 14.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.846
IPC_protein 3.795
Toseland 3.617
ProMoST 3.897
Dawson 3.77
Bjellqvist 3.961
Wikipedia 3.668
Rodwell 3.63
Grimsley 3.528
Solomon 3.757
Lehninger 3.706
Nozaki 3.884
DTASelect 4.037
Thurlkill 3.656
EMBOSS 3.681
Sillero 3.91
Patrickios 0.985
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.84
Protein with the highest isoelectric point:
>tr|A0A5R9GXZ2|A0A5R9GXZ2_9PROT Chaperone SurA OS=Mariprofundus erugo OX=2528639 GN=surA PE=3 SV=1
MM1 pKa = 7.5 SFTYY5 pKa = 9.9 KK6 pKa = 10.15 PSRR9 pKa = 11.84 IRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.68 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.27 AGRR28 pKa = 11.84 LVLSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.84 GRR39 pKa = 11.84 KK40 pKa = 8.55 RR41 pKa = 11.84 LSVV44 pKa = 3.2
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.463
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.384
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2574
0
2574
852991
37
2427
331.4
36.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.662 ± 0.066
1.158 ± 0.018
5.625 ± 0.033
6.08 ± 0.047
3.561 ± 0.03
7.575 ± 0.044
2.781 ± 0.024
5.894 ± 0.035
3.744 ± 0.037
10.557 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.104 ± 0.023
2.957 ± 0.029
4.295 ± 0.033
4.145 ± 0.039
6.263 ± 0.04
5.967 ± 0.036
4.841 ± 0.04
6.989 ± 0.047
1.291 ± 0.018
2.511 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here