Porphyridium purpureum (Red alga) (Porphyridium cruentum)

Taxonomy: cellular organisms; Eukaryota; Rhodophyta; Bangiophyceae; Porphyridiales; Porphyridiaceae; Porphyridium

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9673 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J4YMY5|A0A5J4YMY5_PORPP Uncharacterized protein OS=Porphyridium purpureum OX=35688 GN=FVE85_8505 PE=4 SV=1
MM1 pKa = 8.07LMFLGCVAAVPWVNQLPLDD20 pKa = 4.04EE21 pKa = 5.29TSTACCVSSFTVDD34 pKa = 3.1AEE36 pKa = 4.41GFGNVEE42 pKa = 4.22LVASVGMTDD51 pKa = 3.52GTAGVPEE58 pKa = 4.23ATNQGGIDD66 pKa = 3.33MVIRR70 pKa = 11.84VMGAPFRR77 pKa = 11.84GDD79 pKa = 3.0GDD81 pKa = 3.51VGGQQTSLGSILIGTDD97 pKa = 3.33GEE99 pKa = 5.03DD100 pKa = 2.8IATNVAFDD108 pKa = 3.6AQANQLVVIGTTTGNLAGGDD128 pKa = 4.06GFPQSQRR135 pKa = 11.84DD136 pKa = 3.56LFMAFIDD143 pKa = 3.83VTVEE147 pKa = 3.79NPNQVTLAVNTVVQCGPGEE166 pKa = 4.26GTNTPFALLRR176 pKa = 11.84LPGSVRR182 pKa = 11.84WLGLANSAATEE193 pKa = 4.01NGPFPTGLGFIRR205 pKa = 11.84SDD207 pKa = 4.06DD208 pKa = 3.71VCNGNNFQQKK218 pKa = 9.88NGNTDD223 pKa = 3.51GYY225 pKa = 10.66FSGYY229 pKa = 9.6AAAVKK234 pKa = 9.71TGEE237 pKa = 4.25DD238 pKa = 3.25VFAYY242 pKa = 10.11VYY244 pKa = 10.39EE245 pKa = 4.46VATNPSGGQVYY256 pKa = 9.63RR257 pKa = 11.84AEE259 pKa = 4.08QYY261 pKa = 10.86VLQDD265 pKa = 2.84GDD267 pKa = 3.84FNEE270 pKa = 4.36EE271 pKa = 3.55FRR273 pKa = 11.84IDD275 pKa = 4.85LEE277 pKa = 4.3FDD279 pKa = 3.51PLGQGGLAFEE289 pKa = 4.96SSSITYY295 pKa = 8.1DD296 pKa = 3.12TDD298 pKa = 3.2NSLFALAGVRR308 pKa = 11.84PGYY311 pKa = 11.01LNTEE315 pKa = 3.68HH316 pKa = 7.56DD317 pKa = 3.73AAEE320 pKa = 4.48TEE322 pKa = 4.31VFTYY326 pKa = 10.4YY327 pKa = 11.18AATFTDD333 pKa = 4.82DD334 pKa = 3.13SDD336 pKa = 5.5LNVEE340 pKa = 4.32VAGDD344 pKa = 3.84STDD347 pKa = 3.17GGTVIPVQPVVISAGGSAYY366 pKa = 10.19AAYY369 pKa = 8.6TKK371 pKa = 10.29SRR373 pKa = 11.84RR374 pKa = 11.84DD375 pKa = 3.41RR376 pKa = 11.84VPWAGRR382 pKa = 11.84IFDD385 pKa = 4.56LPSLSFNEE393 pKa = 4.1GDD395 pKa = 4.15DD396 pKa = 3.83FLSVGNLTDD405 pKa = 5.81DD406 pKa = 3.43IVNQIVGFIAPRR418 pKa = 11.84TSQTSFSGFYY428 pKa = 10.38YY429 pKa = 10.9GMAFTPEE436 pKa = 4.28EE437 pKa = 4.15NPFNCVGGPDD447 pKa = 3.28RR448 pKa = 11.84KK449 pKa = 10.41DD450 pKa = 2.99FAMAGLGFSGDD461 pKa = 3.62NVSEE465 pKa = 4.09SWIFTVPSQCSEE477 pKa = 4.25VAVDD481 pKa = 4.8AVLQASVSSDD491 pKa = 2.86RR492 pKa = 11.84PIFVTGTTTGSIAGTTQPTNGGATTTQWTMRR523 pKa = 11.84IEE525 pKa = 4.65PGTGQLPPLNPLCDD539 pKa = 4.62LPCQSIPPPTPSPTPSPTPSPTLSPSPTPDD569 pKa = 3.27ANQTASPSPSPSPSPTPTPTIGSLPVSDD597 pKa = 4.61SVDD600 pKa = 3.17GGVIAGAVVGAIAGSALIVGIGAWIYY626 pKa = 10.71KK627 pKa = 9.51YY628 pKa = 10.75KK629 pKa = 10.45SDD631 pKa = 3.48QAAEE635 pKa = 4.38GGFGEE640 pKa = 4.61TFEE643 pKa = 4.34

Molecular weight: 66.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J4Z810|A0A5J4Z810_PORPP ATP-dependent RNA helicase eIF4A OS=Porphyridium purpureum OX=35688 GN=FVE85_6825 PE=3 SV=1
MM1 pKa = 7.26FGWVHH6 pKa = 5.93SVPPRR11 pKa = 11.84LQPHH15 pKa = 4.68QTPSFGNVCAGRR27 pKa = 11.84FARR30 pKa = 11.84FDD32 pKa = 3.56VPNGQTRR39 pKa = 11.84ARR41 pKa = 11.84ARR43 pKa = 11.84ARR45 pKa = 11.84TAPEE49 pKa = 3.99LPPRR53 pKa = 11.84WTRR56 pKa = 11.84TTVRR60 pKa = 11.84RR61 pKa = 11.84KK62 pKa = 9.77DD63 pKa = 3.24AAVPRR68 pKa = 11.84TEE70 pKa = 4.11QFPFPVMNEE79 pKa = 3.63ALKK82 pKa = 10.35QVPTAKK88 pKa = 10.59AMAVLLCIDD97 pKa = 4.48QIFKK101 pKa = 10.89NAFARR106 pKa = 11.84YY107 pKa = 7.9NWCFPSALAAMCALLGALLMLDD129 pKa = 3.32RR130 pKa = 11.84VSPDD134 pKa = 2.78SAEE137 pKa = 3.96NVVGFFQPVCAFFSRR152 pKa = 11.84WLAVFFVPVLIALPVSARR170 pKa = 11.84PWPAADD176 pKa = 3.83EE177 pKa = 4.15LAKK180 pKa = 10.44IAVVLVGGLAISLGFTAKK198 pKa = 10.39LCALLRR204 pKa = 11.84AASAGAEE211 pKa = 4.07KK212 pKa = 10.54DD213 pKa = 3.48GQEE216 pKa = 4.13QVSLAGAASVPLSTGAPSASATSFPCANLTRR247 pKa = 11.84FWGGVALLSFCSGVMFPAVQPLRR270 pKa = 11.84LVFTFATTLFGFCMGQRR287 pKa = 11.84AAPSVKK293 pKa = 10.02RR294 pKa = 11.84LVHH297 pKa = 5.92PLVATVLTSFLAFSLFGRR315 pKa = 11.84LSGIGFFNVVGEE327 pKa = 4.19YY328 pKa = 10.12FSKK331 pKa = 10.75GKK333 pKa = 10.55CGACLGGGDD342 pKa = 5.04LLMMILGPAIVSFAFHH358 pKa = 6.53IFNRR362 pKa = 11.84RR363 pKa = 11.84ALIKK367 pKa = 9.47RR368 pKa = 11.84HH369 pKa = 5.31APEE372 pKa = 4.23VVGTTALSSFVSLFATAYY390 pKa = 9.79VGRR393 pKa = 11.84LLNLPANIRR402 pKa = 11.84LSLIPRR408 pKa = 11.84MVTAPLAICIGDD420 pKa = 4.63LLHH423 pKa = 7.49ADD425 pKa = 3.76PALAGALAATTGLLGANFYY444 pKa = 11.85AMVLDD449 pKa = 4.7GLQITDD455 pKa = 4.81PIYY458 pKa = 10.62RR459 pKa = 11.84GISTGSASHH468 pKa = 7.01GIGSAAILKK477 pKa = 9.73EE478 pKa = 3.95GEE480 pKa = 4.19PFAFAAIAMVLCGVFSTVFISIKK503 pKa = 8.98PLRR506 pKa = 11.84AALIARR512 pKa = 11.84RR513 pKa = 11.84MTGHH517 pKa = 7.63PLNSIPRR524 pKa = 11.84LLPRR528 pKa = 11.84SLPGTPASTRR538 pKa = 11.84KK539 pKa = 9.57QSLAIAPSPDD549 pKa = 3.0VRR551 pKa = 11.84CRR553 pKa = 11.84TNNRR557 pKa = 11.84GSGRR561 pKa = 11.84VNEE564 pKa = 4.47VGGAMRR570 pKa = 11.84FRR572 pKa = 11.84CRR574 pKa = 11.84SLVVSPRR581 pKa = 11.84CAYY584 pKa = 9.73IPSNSNFSSS593 pKa = 3.32

Molecular weight: 63.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9673

0

9673

4998014

50

6419

516.7

56.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.5 ± 0.025

1.693 ± 0.009

5.538 ± 0.015

6.402 ± 0.019

3.605 ± 0.015

6.964 ± 0.021

2.537 ± 0.012

3.645 ± 0.013

4.234 ± 0.022

9.368 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.54 ± 0.009

2.927 ± 0.011

4.803 ± 0.016

4.033 ± 0.016

7.393 ± 0.022

8.124 ± 0.025

4.849 ± 0.012

7.356 ± 0.017

1.273 ± 0.008

2.215 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski