Amino acid dipepetide frequency for Porphyridium purpureum (Red alga) (Porphyridium cruentum)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.816AlaAla: 12.816 ± 0.09
1.84AlaCys: 1.84 ± 0.024
5.174AlaAsp: 5.174 ± 0.036
6.427AlaGlu: 6.427 ± 0.048
3.737AlaPhe: 3.737 ± 0.027
7.138AlaGly: 7.138 ± 0.05
2.629AlaHis: 2.629 ± 0.023
3.359AlaIle: 3.359 ± 0.025
3.976AlaLys: 3.976 ± 0.038
10.592AlaLeu: 10.592 ± 0.064
2.5AlaMet: 2.5 ± 0.021
2.771AlaAsn: 2.771 ± 0.023
5.235AlaPro: 5.235 ± 0.044
4.482AlaGln: 4.482 ± 0.034
8.047AlaArg: 8.047 ± 0.054
8.958AlaSer: 8.958 ± 0.058
4.749AlaThr: 4.749 ± 0.035
7.13AlaVal: 7.13 ± 0.04
1.285AlaTrp: 1.285 ± 0.017
2.153AlaTyr: 2.153 ± 0.021
0.0AlaXaa: 0.0 ± 0.0
Cys
1.898CysAla: 1.898 ± 0.025
0.349CysCys: 0.349 ± 0.009
0.941CysAsp: 0.941 ± 0.014
1.014CysGlu: 1.014 ± 0.016
0.646CysPhe: 0.646 ± 0.012
1.446CysGly: 1.446 ± 0.02
0.386CysHis: 0.386 ± 0.009
0.699CysIle: 0.699 ± 0.013
0.601CysLys: 0.601 ± 0.012
1.429CysLeu: 1.429 ± 0.021
0.462CysMet: 0.462 ± 0.01
0.414CysAsn: 0.414 ± 0.01
0.685CysPro: 0.685 ± 0.013
0.442CysGln: 0.442 ± 0.01
1.098CysArg: 1.098 ± 0.015
1.347CysSer: 1.347 ± 0.021
0.903CysThr: 0.903 ± 0.013
1.617CysVal: 1.617 ± 0.018
0.219CysTrp: 0.219 ± 0.007
0.337CysTyr: 0.337 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
6.84AspAla: 6.84 ± 0.039
0.746AspCys: 0.746 ± 0.012
3.788AspAsp: 3.788 ± 0.04
4.533AspGlu: 4.533 ± 0.036
2.13AspPhe: 2.13 ± 0.021
4.306AspGly: 4.306 ± 0.035
1.147AspHis: 1.147 ± 0.016
2.159AspIle: 2.159 ± 0.023
1.964AspLys: 1.964 ± 0.022
4.801AspLeu: 4.801 ± 0.035
1.386AspMet: 1.386 ± 0.017
1.259AspAsn: 1.259 ± 0.018
2.525AspPro: 2.525 ± 0.025
1.729AspGln: 1.729 ± 0.022
3.258AspArg: 3.258 ± 0.027
3.978AspSer: 3.978 ± 0.032
2.647AspThr: 2.647 ± 0.025
4.319AspVal: 4.319 ± 0.033
0.767AspTrp: 0.767 ± 0.013
1.102AspTyr: 1.102 ± 0.018
0.0AspXaa: 0.0 ± 0.0
Glu
5.905GluAla: 5.905 ± 0.04
0.969GluCys: 0.969 ± 0.016
3.416GluAsp: 3.416 ± 0.031
4.555GluGlu: 4.555 ± 0.044
2.233GluPhe: 2.233 ± 0.022
3.273GluGly: 3.273 ± 0.026
1.895GluHis: 1.895 ± 0.021
2.469GluIle: 2.469 ± 0.022
3.29GluLys: 3.29 ± 0.034
6.831GluLeu: 6.831 ± 0.05
1.754GluMet: 1.754 ± 0.018
2.239GluAsn: 2.239 ± 0.022
2.78GluPro: 2.78 ± 0.026
3.256GluGln: 3.256 ± 0.033
5.207GluArg: 5.207 ± 0.042
4.722GluSer: 4.722 ± 0.035
3.077GluThr: 3.077 ± 0.026
3.636GluVal: 3.636 ± 0.03
0.859GluTrp: 0.859 ± 0.016
1.652GluTyr: 1.652 ± 0.018
0.0GluXaa: 0.0 ± 0.0
Phe
3.543PheAla: 3.543 ± 0.03
0.727PheCys: 0.727 ± 0.012
2.199PheAsp: 2.199 ± 0.022
2.445PheGlu: 2.445 ± 0.021
1.459PhePhe: 1.459 ± 0.018
2.96PheGly: 2.96 ± 0.034
0.772PheHis: 0.772 ± 0.013
1.281PheIle: 1.281 ± 0.019
1.289PheLys: 1.289 ± 0.017
3.375PheLeu: 3.375 ± 0.028
0.833PheMet: 0.833 ± 0.014
0.986PheAsn: 0.986 ± 0.019
1.481PhePro: 1.481 ± 0.018
1.304PheGln: 1.304 ± 0.02
2.381PheArg: 2.381 ± 0.024
2.797PheSer: 2.797 ± 0.031
1.555PheThr: 1.555 ± 0.017
3.261PheVal: 3.261 ± 0.029
0.514PheTrp: 0.514 ± 0.011
0.887PheTyr: 0.887 ± 0.015
0.0PheXaa: 0.0 ± 0.0
Gly
7.612GlyAla: 7.612 ± 0.044
1.074GlyCys: 1.074 ± 0.015
3.506GlyAsp: 3.506 ± 0.029
3.823GlyGlu: 3.823 ± 0.032
2.501GlyPhe: 2.501 ± 0.025
5.792GlyGly: 5.792 ± 0.06
1.7GlyHis: 1.7 ± 0.024
2.755GlyIle: 2.755 ± 0.026
3.236GlyLys: 3.236 ± 0.028
5.81GlyLeu: 5.81 ± 0.036
1.929GlyMet: 1.929 ± 0.024
2.141GlyAsn: 2.141 ± 0.019
2.838GlyPro: 2.838 ± 0.026
2.311GlyGln: 2.311 ± 0.021
4.703GlyArg: 4.703 ± 0.041
6.439GlySer: 6.439 ± 0.043
3.949GlyThr: 3.949 ± 0.031
5.069GlyVal: 5.069 ± 0.039
0.948GlyTrp: 0.948 ± 0.013
1.504GlyTyr: 1.504 ± 0.022
0.0GlyXaa: 0.0 ± 0.0
His
3.276HisAla: 3.276 ± 0.03
0.439HisCys: 0.439 ± 0.009
1.413HisAsp: 1.413 ± 0.02
1.754HisGlu: 1.754 ± 0.02
0.892HisPhe: 0.892 ± 0.016
2.031HisGly: 2.031 ± 0.021
0.711HisHis: 0.711 ± 0.015
0.897HisIle: 0.897 ± 0.012
0.827HisLys: 0.827 ± 0.014
2.072HisLeu: 2.072 ± 0.017
0.617HisMet: 0.617 ± 0.013
0.589HisAsn: 0.589 ± 0.011
1.254HisPro: 1.254 ± 0.019
0.88HisGln: 0.88 ± 0.015
1.651HisArg: 1.651 ± 0.018
1.772HisSer: 1.772 ± 0.023
1.224HisThr: 1.224 ± 0.019
2.275HisVal: 2.275 ± 0.023
0.334HisTrp: 0.334 ± 0.009
0.466HisTyr: 0.466 ± 0.01
0.0HisXaa: 0.0 ± 0.0
Ile
3.523IleAla: 3.523 ± 0.027
0.714IleCys: 0.714 ± 0.012
2.226IleAsp: 2.226 ± 0.022
2.526IleGlu: 2.526 ± 0.026
1.382IlePhe: 1.382 ± 0.019
2.448IleGly: 2.448 ± 0.027
0.921IleHis: 0.921 ± 0.013
1.313IleIle: 1.313 ± 0.016
1.429IleLys: 1.429 ± 0.019
3.555IleLeu: 3.555 ± 0.03
0.931IleMet: 0.931 ± 0.014
0.949IleAsn: 0.949 ± 0.013
1.958IlePro: 1.958 ± 0.02
1.439IleGln: 1.439 ± 0.016
2.581IleArg: 2.581 ± 0.023
2.796IleSer: 2.796 ± 0.027
1.64IleThr: 1.64 ± 0.022
2.876IleVal: 2.876 ± 0.027
0.461IleTrp: 0.461 ± 0.01
0.787IleTyr: 0.787 ± 0.013
0.0IleXaa: 0.0 ± 0.0
Lys
3.726LysAla: 3.726 ± 0.035
0.655LysCys: 0.655 ± 0.012
2.198LysAsp: 2.198 ± 0.027
2.67LysGlu: 2.67 ± 0.027
1.324LysPhe: 1.324 ± 0.019
2.42LysGly: 2.42 ± 0.028
1.152LysHis: 1.152 ± 0.015
1.585LysIle: 1.585 ± 0.02
2.56LysLys: 2.56 ± 0.04
3.954LysLeu: 3.954 ± 0.035
1.203LysMet: 1.203 ± 0.016
1.398LysAsn: 1.398 ± 0.017
1.993LysPro: 1.993 ± 0.022
1.905LysGln: 1.905 ± 0.024
3.565LysArg: 3.565 ± 0.031
3.207LysSer: 3.207 ± 0.032
2.178LysThr: 2.178 ± 0.024
2.946LysVal: 2.946 ± 0.028
0.52LysTrp: 0.52 ± 0.01
1.176LysTyr: 1.176 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
9.203LeuAla: 9.203 ± 0.044
1.784LeuCys: 1.784 ± 0.021
5.528LeuAsp: 5.528 ± 0.033
6.145LeuGlu: 6.145 ± 0.048
3.442LeuPhe: 3.442 ± 0.029
6.278LeuGly: 6.278 ± 0.041
2.558LeuHis: 2.558 ± 0.025
3.199LeuIle: 3.199 ± 0.029
4.013LeuLys: 4.013 ± 0.039
9.167LeuLeu: 9.167 ± 0.063
2.201LeuMet: 2.201 ± 0.025
2.839LeuAsn: 2.839 ± 0.021
4.565LeuPro: 4.565 ± 0.035
3.936LeuGln: 3.936 ± 0.03
7.366LeuArg: 7.366 ± 0.04
7.233LeuSer: 7.233 ± 0.051
4.058LeuThr: 4.058 ± 0.032
6.983LeuVal: 6.983 ± 0.048
1.098LeuTrp: 1.098 ± 0.016
2.086LeuTyr: 2.086 ± 0.021
0.0LeuXaa: 0.0 ± 0.0
Met
2.468MetAla: 2.468 ± 0.022
0.446MetCys: 0.446 ± 0.009
1.546MetAsp: 1.546 ± 0.017
1.606MetGlu: 1.606 ± 0.022
0.859MetPhe: 0.859 ± 0.018
1.544MetGly: 1.544 ± 0.02
0.7MetHis: 0.7 ± 0.013
0.918MetIle: 0.918 ± 0.014
1.078MetLys: 1.078 ± 0.015
2.428MetLeu: 2.428 ± 0.023
0.727MetMet: 0.727 ± 0.013
0.884MetAsn: 0.884 ± 0.013
1.243MetPro: 1.243 ± 0.019
1.219MetGln: 1.219 ± 0.018
2.021MetArg: 2.021 ± 0.019
1.937MetSer: 1.937 ± 0.022
1.227MetThr: 1.227 ± 0.016
1.566MetVal: 1.566 ± 0.019
0.312MetTrp: 0.312 ± 0.009
0.674MetTyr: 0.674 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
3.409AsnAla: 3.409 ± 0.025
0.461AsnCys: 0.461 ± 0.01
1.609AsnAsp: 1.609 ± 0.02
1.967AsnGlu: 1.967 ± 0.023
0.999AsnPhe: 0.999 ± 0.015
2.319AsnGly: 2.319 ± 0.026
0.665AsnHis: 0.665 ± 0.013
1.161AsnIle: 1.161 ± 0.016
1.108AsnLys: 1.108 ± 0.016
2.543AsnLeu: 2.543 ± 0.024
0.815AsnMet: 0.815 ± 0.013
0.77AsnAsn: 0.77 ± 0.015
1.506AsnPro: 1.506 ± 0.022
1.051AsnGln: 1.051 ± 0.016
1.805AsnArg: 1.805 ± 0.017
2.106AsnSer: 2.106 ± 0.024
1.47AsnThr: 1.47 ± 0.02
2.512AsnVal: 2.512 ± 0.026
0.362AsnTrp: 0.362 ± 0.009
0.636AsnTyr: 0.636 ± 0.012
0.0AsnXaa: 0.0 ± 0.0
Pro
4.872ProAla: 4.872 ± 0.038
0.74ProCys: 0.74 ± 0.014
2.775ProAsp: 2.775 ± 0.024
3.238ProGlu: 3.238 ± 0.027
1.758ProPhe: 1.758 ± 0.023
3.379ProGly: 3.379 ± 0.03
1.176ProHis: 1.176 ± 0.02
1.507ProIle: 1.507 ± 0.019
1.831ProLys: 1.831 ± 0.023
4.07ProLeu: 4.07 ± 0.032
0.985ProMet: 0.985 ± 0.015
1.485ProAsn: 1.485 ± 0.02
2.978ProPro: 2.978 ± 0.036
1.959ProGln: 1.959 ± 0.024
3.418ProArg: 3.418 ± 0.031
4.356ProSer: 4.356 ± 0.042
2.377ProThr: 2.377 ± 0.027
3.503ProVal: 3.503 ± 0.029
0.589ProTrp: 0.589 ± 0.011
1.033ProTyr: 1.033 ± 0.015
0.0ProXaa: 0.0 ± 0.0
Gln
3.816GlnAla: 3.816 ± 0.032
0.604GlnCys: 0.604 ± 0.012
2.105GlnAsp: 2.105 ± 0.023
2.414GlnGlu: 2.414 ± 0.025
1.221GlnPhe: 1.221 ± 0.016
2.37GlnGly: 2.37 ± 0.022
1.206GlnHis: 1.206 ± 0.018
1.53GlnIle: 1.53 ± 0.02
1.82GlnLys: 1.82 ± 0.023
3.804GlnLeu: 3.804 ± 0.033
1.053GlnMet: 1.053 ± 0.014
1.434GlnAsn: 1.434 ± 0.018
1.894GlnPro: 1.894 ± 0.025
2.75GlnGln: 2.75 ± 0.062
3.258GlnArg: 3.258 ± 0.028
2.858GlnSer: 2.858 ± 0.025
2.007GlnThr: 2.007 ± 0.021
2.763GlnVal: 2.763 ± 0.024
0.49GlnTrp: 0.49 ± 0.01
0.934GlnTyr: 0.934 ± 0.016
0.0GlnXaa: 0.0 ± 0.0
Arg
8.011ArgAla: 8.011 ± 0.048
1.183ArgCys: 1.183 ± 0.018
3.893ArgAsp: 3.893 ± 0.037
4.664ArgGlu: 4.664 ± 0.041
2.608ArgPhe: 2.608 ± 0.025
4.777ArgGly: 4.777 ± 0.039
1.806ArgHis: 1.806 ± 0.02
2.946ArgIle: 2.946 ± 0.027
3.718ArgLys: 3.718 ± 0.037
6.712ArgLeu: 6.712 ± 0.036
2.048ArgMet: 2.048 ± 0.021
2.259ArgAsn: 2.259 ± 0.023
3.097ArgPro: 3.097 ± 0.033
2.731ArgGln: 2.731 ± 0.026
6.056ArgArg: 6.056 ± 0.049
5.662ArgSer: 5.662 ± 0.043
3.896ArgThr: 3.896 ± 0.028
5.464ArgVal: 5.464 ± 0.034
0.9ArgTrp: 0.9 ± 0.015
1.502ArgTyr: 1.502 ± 0.021
0.0ArgXaa: 0.0 ± 0.0
Ser
8.892SerAla: 8.892 ± 0.062
1.233SerCys: 1.233 ± 0.018
4.391SerAsp: 4.391 ± 0.038
4.856SerGlu: 4.856 ± 0.036
2.873SerPhe: 2.873 ± 0.027
6.502SerGly: 6.502 ± 0.044
1.825SerHis: 1.825 ± 0.019
2.852SerIle: 2.852 ± 0.026
3.261SerLys: 3.261 ± 0.032
6.78SerLeu: 6.78 ± 0.048
2.088SerMet: 2.088 ± 0.02
2.309SerAsn: 2.309 ± 0.023
3.867SerPro: 3.867 ± 0.044
2.811SerGln: 2.811 ± 0.027
5.755SerArg: 5.755 ± 0.042
8.513SerSer: 8.513 ± 0.076
4.188SerThr: 4.188 ± 0.036
5.874SerVal: 5.874 ± 0.038
0.966SerTrp: 0.966 ± 0.017
1.402SerTyr: 1.402 ± 0.016
0.0SerXaa: 0.0 ± 0.0
Thr
4.872ThrAla: 4.872 ± 0.038
0.901ThrCys: 0.901 ± 0.015
2.521ThrAsp: 2.521 ± 0.023
2.845ThrGlu: 2.845 ± 0.026
1.748ThrPhe: 1.748 ± 0.023
3.693ThrGly: 3.693 ± 0.032
1.262ThrHis: 1.262 ± 0.017
1.756ThrIle: 1.756 ± 0.021
1.952ThrLys: 1.952 ± 0.022
4.654ThrLeu: 4.654 ± 0.032
1.158ThrMet: 1.158 ± 0.018
1.388ThrAsn: 1.388 ± 0.018
2.734ThrPro: 2.734 ± 0.029
1.851ThrGln: 1.851 ± 0.02
3.532ThrArg: 3.532 ± 0.027
4.095ThrSer: 4.095 ± 0.034
2.302ThrThr: 2.302 ± 0.023
3.445ThrVal: 3.445 ± 0.029
0.663ThrTrp: 0.663 ± 0.012
1.122ThrTyr: 1.122 ± 0.016
0.0ThrXaa: 0.0 ± 0.0
Val
7.151ValAla: 7.151 ± 0.042
1.438ValCys: 1.438 ± 0.019
4.158ValAsp: 4.158 ± 0.03
4.467ValGlu: 4.467 ± 0.031
2.772ValPhe: 2.772 ± 0.026
4.613ValGly: 4.613 ± 0.03
2.032ValHis: 2.032 ± 0.023
2.65ValIle: 2.65 ± 0.028
2.83ValLys: 2.83 ± 0.028
7.699ValLeu: 7.699 ± 0.048
1.696ValMet: 1.696 ± 0.018
1.915ValAsn: 1.915 ± 0.019
3.935ValPro: 3.935 ± 0.032
3.08ValGln: 3.08 ± 0.03
5.733ValArg: 5.733 ± 0.038
5.814ValSer: 5.814 ± 0.036
3.241ValThr: 3.241 ± 0.029
5.658ValVal: 5.658 ± 0.044
0.924ValTrp: 0.924 ± 0.014
1.754ValTyr: 1.754 ± 0.019
0.0ValXaa: 0.0 ± 0.0
Trp
0.928TrpAla: 0.928 ± 0.016
0.229TrpCys: 0.229 ± 0.007
0.738TrpAsp: 0.738 ± 0.019
0.622TrpGlu: 0.622 ± 0.013
0.536TrpPhe: 0.536 ± 0.011
0.602TrpGly: 0.602 ± 0.011
0.319TrpHis: 0.319 ± 0.008
0.572TrpIle: 0.572 ± 0.01
0.668TrpLys: 0.668 ± 0.012
1.306TrpLeu: 1.306 ± 0.018
0.397TrpMet: 0.397 ± 0.008
0.567TrpAsn: 0.567 ± 0.011
0.528TrpPro: 0.528 ± 0.01
0.495TrpGln: 0.495 ± 0.01
1.014TrpArg: 1.014 ± 0.016
1.032TrpSer: 1.032 ± 0.014
0.729TrpThr: 0.729 ± 0.012
0.842TrpVal: 0.842 ± 0.014
0.234TrpTrp: 0.234 ± 0.007
0.37TrpTyr: 0.37 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.238TyrAla: 2.238 ± 0.021
0.4TyrCys: 0.4 ± 0.01
1.261TyrAsp: 1.261 ± 0.017
1.45TyrGlu: 1.45 ± 0.018
0.928TyrPhe: 0.928 ± 0.015
1.749TyrGly: 1.749 ± 0.023
0.511TyrHis: 0.511 ± 0.01
0.845TyrIle: 0.845 ± 0.014
0.885TyrLys: 0.885 ± 0.016
2.1TyrLeu: 2.1 ± 0.02
0.618TyrMet: 0.618 ± 0.01
0.676TyrAsn: 0.676 ± 0.012
0.932TyrPro: 0.932 ± 0.014
0.705TyrGln: 0.705 ± 0.01
1.483TyrArg: 1.483 ± 0.019
1.616TyrSer: 1.616 ± 0.019
1.078TyrThr: 1.078 ± 0.016
1.819TyrVal: 1.819 ± 0.018
0.283TyrTrp: 0.283 ± 0.009
0.573TyrTyr: 0.573 ± 0.011
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 9673 proteins (4998014 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski