Glaciecola punicea ACAM 611
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2883 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H5TD77|H5TD77_9ALTE Uncharacterized protein OS=Glaciecola punicea ACAM 611 OX=1121923 GN=GPUN_2139 PE=4 SV=1
MM1 pKa = 7.56 TKK3 pKa = 9.83 PISIGIFYY11 pKa = 10.69 GSTTCYY17 pKa = 10.71 TEE19 pKa = 3.84 MASEE23 pKa = 5.39 KK24 pKa = 10.22 IQQQLQALLLQGDD37 pKa = 3.98 VVGQVEE43 pKa = 4.74 LFDD46 pKa = 4.62 IKK48 pKa = 10.55 DD49 pKa = 3.22 TALAKK54 pKa = 9.82 SQNYY58 pKa = 9.68 DD59 pKa = 3.36 VILYY63 pKa = 9.36 GISTWDD69 pKa = 3.39 FGEE72 pKa = 4.3 LQEE75 pKa = 5.12 DD76 pKa = 4.44 WEE78 pKa = 4.77 STWDD82 pKa = 4.59 DD83 pKa = 3.27 IATLEE88 pKa = 4.31 LSGKK92 pKa = 9.63 VVGLFGLGDD101 pKa = 3.54 QLGYY105 pKa = 11.22 ADD107 pKa = 4.61 WFQDD111 pKa = 3.71 ALGMLHH117 pKa = 7.55 DD118 pKa = 4.59 EE119 pKa = 6.1 LILLDD124 pKa = 4.83 CDD126 pKa = 5.94 LIGYY130 pKa = 7.48 WPNEE134 pKa = 3.88 GYY136 pKa = 10.91 EE137 pKa = 4.33 FVASKK142 pKa = 11.03 GLTPDD147 pKa = 2.99 EE148 pKa = 4.11 QFFVGLSLDD157 pKa = 4.67 DD158 pKa = 3.97 EE159 pKa = 4.56 NQYY162 pKa = 10.78 EE163 pKa = 4.67 LSDD166 pKa = 3.53 TRR168 pKa = 11.84 IKK170 pKa = 10.38 LWCKK174 pKa = 9.34 QVIDD178 pKa = 5.0 QIHH181 pKa = 6.36 EE182 pKa = 4.06 LNAAA186 pKa = 3.67
Molecular weight: 21.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.783
IPC_protein 3.77
Toseland 3.567
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.694
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.706
Nozaki 3.884
DTASelect 4.101
Thurlkill 3.617
EMBOSS 3.706
Sillero 3.897
Patrickios 1.062
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.8
Protein with the highest isoelectric point:
>tr|H5TDZ7|H5TDZ7_9ALTE Protein adenylyltransferase SelO OS=Glaciecola punicea ACAM 611 OX=1121923 GN=selO PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.14 RR12 pKa = 11.84 KK13 pKa = 9.13 RR14 pKa = 11.84 SHH16 pKa = 6.17 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.38 NGRR28 pKa = 11.84 KK29 pKa = 9.48 VIANRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.83 GRR39 pKa = 11.84 KK40 pKa = 8.87 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2883
0
2883
882842
37
1612
306.2
33.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.9 ± 0.043
1.043 ± 0.017
5.475 ± 0.035
5.561 ± 0.042
4.337 ± 0.033
6.691 ± 0.04
2.222 ± 0.023
6.904 ± 0.032
5.564 ± 0.038
10.131 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.59 ± 0.023
4.69 ± 0.035
3.798 ± 0.023
4.459 ± 0.035
4.338 ± 0.035
6.939 ± 0.037
5.36 ± 0.027
6.883 ± 0.038
1.084 ± 0.016
3.031 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here