Glaciecola punicea ACAM 611

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Glaciecola; Glaciecola punicea

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2883 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|H5TD77|H5TD77_9ALTE Uncharacterized protein OS=Glaciecola punicea ACAM 611 OX=1121923 GN=GPUN_2139 PE=4 SV=1
MM1 pKa = 7.56TKK3 pKa = 9.83PISIGIFYY11 pKa = 10.69GSTTCYY17 pKa = 10.71TEE19 pKa = 3.84MASEE23 pKa = 5.39KK24 pKa = 10.22IQQQLQALLLQGDD37 pKa = 3.98VVGQVEE43 pKa = 4.74LFDD46 pKa = 4.62IKK48 pKa = 10.55DD49 pKa = 3.22TALAKK54 pKa = 9.82SQNYY58 pKa = 9.68DD59 pKa = 3.36VILYY63 pKa = 9.36GISTWDD69 pKa = 3.39FGEE72 pKa = 4.3LQEE75 pKa = 5.12DD76 pKa = 4.44WEE78 pKa = 4.77STWDD82 pKa = 4.59DD83 pKa = 3.27IATLEE88 pKa = 4.31LSGKK92 pKa = 9.63VVGLFGLGDD101 pKa = 3.54QLGYY105 pKa = 11.22ADD107 pKa = 4.61WFQDD111 pKa = 3.71ALGMLHH117 pKa = 7.55DD118 pKa = 4.59EE119 pKa = 6.1LILLDD124 pKa = 4.83CDD126 pKa = 5.94LIGYY130 pKa = 7.48WPNEE134 pKa = 3.88GYY136 pKa = 10.91EE137 pKa = 4.33FVASKK142 pKa = 11.03GLTPDD147 pKa = 2.99EE148 pKa = 4.11QFFVGLSLDD157 pKa = 4.67DD158 pKa = 3.97EE159 pKa = 4.56NQYY162 pKa = 10.78EE163 pKa = 4.67LSDD166 pKa = 3.53TRR168 pKa = 11.84IKK170 pKa = 10.38LWCKK174 pKa = 9.34QVIDD178 pKa = 5.0QIHH181 pKa = 6.36EE182 pKa = 4.06LNAAA186 pKa = 3.67

Molecular weight:
21.01 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|H5TDZ7|H5TDZ7_9ALTE Protein adenylyltransferase SelO OS=Glaciecola punicea ACAM 611 OX=1121923 GN=selO PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.14RR12 pKa = 11.84KK13 pKa = 9.13RR14 pKa = 11.84SHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38NGRR28 pKa = 11.84KK29 pKa = 9.48VIANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.83GRR39 pKa = 11.84KK40 pKa = 8.87RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight:
5.17 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2883

0

2883

882842

37

1612

306.2

33.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.9 ± 0.043

1.043 ± 0.017

5.475 ± 0.035

5.561 ± 0.042

4.337 ± 0.033

6.691 ± 0.04

2.222 ± 0.023

6.904 ± 0.032

5.564 ± 0.038

10.131 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.59 ± 0.023

4.69 ± 0.035

3.798 ± 0.023

4.459 ± 0.035

4.338 ± 0.035

6.939 ± 0.037

5.36 ± 0.027

6.883 ± 0.038

1.084 ± 0.016

3.031 ± 0.026

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski