Glaciecola punicea ACAM 611 
Average proteome isoelectric point is 6.53 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 2883 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|H5TD77|H5TD77_9ALTE Uncharacterized protein OS=Glaciecola punicea ACAM 611 OX=1121923 GN=GPUN_2139 PE=4 SV=1MM1 pKa = 7.56  TKK3 pKa = 9.83  PISIGIFYY11 pKa = 10.69  GSTTCYY17 pKa = 10.71  TEE19 pKa = 3.84  MASEE23 pKa = 5.39  KK24 pKa = 10.22  IQQQLQALLLQGDD37 pKa = 3.98  VVGQVEE43 pKa = 4.74  LFDD46 pKa = 4.62  IKK48 pKa = 10.55  DD49 pKa = 3.22  TALAKK54 pKa = 9.82  SQNYY58 pKa = 9.68  DD59 pKa = 3.36  VILYY63 pKa = 9.36  GISTWDD69 pKa = 3.39  FGEE72 pKa = 4.3  LQEE75 pKa = 5.12  DD76 pKa = 4.44  WEE78 pKa = 4.77  STWDD82 pKa = 4.59  DD83 pKa = 3.27  IATLEE88 pKa = 4.31  LSGKK92 pKa = 9.63  VVGLFGLGDD101 pKa = 3.54  QLGYY105 pKa = 11.22  ADD107 pKa = 4.61  WFQDD111 pKa = 3.71  ALGMLHH117 pKa = 7.55  DD118 pKa = 4.59  EE119 pKa = 6.1  LILLDD124 pKa = 4.83  CDD126 pKa = 5.94  LIGYY130 pKa = 7.48  WPNEE134 pKa = 3.88  GYY136 pKa = 10.91  EE137 pKa = 4.33  FVASKK142 pKa = 11.03  GLTPDD147 pKa = 2.99  EE148 pKa = 4.11  QFFVGLSLDD157 pKa = 4.67  DD158 pKa = 3.97  EE159 pKa = 4.56  NQYY162 pKa = 10.78  EE163 pKa = 4.67  LSDD166 pKa = 3.53  TRR168 pKa = 11.84  IKK170 pKa = 10.38  LWCKK174 pKa = 9.34  QVIDD178 pKa = 5.0  QIHH181 pKa = 6.36  EE182 pKa = 4.06  LNAAA186 pKa = 3.67  
 21.01 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.702 
IPC2_protein 3.783 
IPC_protein 3.77 
Toseland    3.567 
ProMoST     3.935 
Dawson      3.77 
Bjellqvist  3.923 
Wikipedia   3.694 
Rodwell     3.605 
Grimsley    3.478 
Solomon     3.757 
Lehninger   3.706 
Nozaki      3.884 
DTASelect   4.101 
Thurlkill   3.617 
EMBOSS      3.706 
Sillero     3.897 
Patrickios  1.062 
IPC_peptide 3.757 
IPC2_peptide  3.872 
IPC2.peptide.svr19  3.8 
 Protein with the highest isoelectric point: 
>tr|H5TDZ7|H5TDZ7_9ALTE Protein adenylyltransferase SelO OS=Glaciecola punicea ACAM 611 OX=1121923 GN=selO PE=3 SV=1MM1 pKa = 7.45  KK2 pKa = 9.61  RR3 pKa = 11.84  TFQPSNLKK11 pKa = 10.14  RR12 pKa = 11.84  KK13 pKa = 9.13  RR14 pKa = 11.84  SHH16 pKa = 6.17  GFRR19 pKa = 11.84  ARR21 pKa = 11.84  MATKK25 pKa = 10.38  NGRR28 pKa = 11.84  KK29 pKa = 9.48  VIANRR34 pKa = 11.84  RR35 pKa = 11.84  AKK37 pKa = 9.83  GRR39 pKa = 11.84  KK40 pKa = 8.87  RR41 pKa = 11.84  LSAA44 pKa = 3.96  
 5.17 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.517 
IPC2_protein 11.213 
IPC_protein 12.793 
Toseland    12.969 
ProMoST     13.451 
Dawson      12.969 
Bjellqvist  12.954 
Wikipedia   13.437 
Rodwell     12.705 
Grimsley    12.998 
Solomon     13.451 
Lehninger   13.364 
Nozaki      12.969 
DTASelect   12.954 
Thurlkill   12.969 
EMBOSS      13.466 
Sillero     12.969 
Patrickios  12.427 
IPC_peptide 13.466 
IPC2_peptide  12.442 
IPC2.peptide.svr19  9.142 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        2883 
0
2883 
882842
37
1612
306.2
33.92
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        8.9 ± 0.043
1.043 ± 0.017
5.475 ± 0.035
5.561 ± 0.042
4.337 ± 0.033
6.691 ± 0.04
2.222 ± 0.023
6.904 ± 0.032
5.564 ± 0.038
10.131 ± 0.054
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.59 ± 0.023
4.69 ± 0.035
3.798 ± 0.023
4.459 ± 0.035
4.338 ± 0.035
6.939 ± 0.037
5.36 ± 0.027
6.883 ± 0.038
1.084 ± 0.016
3.031 ± 0.026
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here