Fowl aviadenovirus 2
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A191ULH5|A0A191ULH5_9ADEN ORF1B OS=Fowl aviadenovirus 2 OX=172859 PE=4 SV=1
MM1 pKa = 7.24 VKK3 pKa = 9.61 TGISEE8 pKa = 4.1 EE9 pKa = 4.26 VAKK12 pKa = 10.91 LFATIDD18 pKa = 3.59 DD19 pKa = 4.15 MSIEE23 pKa = 4.12 DD24 pKa = 3.66 WFLVFVVLLAILSFLWGMFLGLLCGPGDD52 pKa = 4.78 LDD54 pKa = 4.07 FAGYY58 pKa = 8.57 WGLVFPCLL66 pKa = 3.97
Molecular weight: 7.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.795
IPC2_protein 3.77
IPC_protein 3.605
Toseland 3.427
ProMoST 3.834
Dawson 3.63
Bjellqvist 3.808
Wikipedia 3.617
Rodwell 3.465
Grimsley 3.35
Solomon 3.567
Lehninger 3.528
Nozaki 3.795
DTASelect 3.961
Thurlkill 3.528
EMBOSS 3.63
Sillero 3.745
Patrickios 1.863
IPC_peptide 3.567
IPC2_peptide 3.706
IPC2.peptide.svr19 3.716
Protein with the highest isoelectric point:
>tr|A0A191ULH1|A0A191ULH1_9ADEN dUTP diphosphatase OS=Fowl aviadenovirus 2 OX=172859 PE=3 SV=1
MM1 pKa = 7.68 SILISPNDD9 pKa = 3.25 NRR11 pKa = 11.84 GWGMRR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 SRR20 pKa = 11.84 SSMRR24 pKa = 11.84 GVGIRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 LTLRR36 pKa = 11.84 TLLGLGTSSRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 SGGRR52 pKa = 11.84 SSRR55 pKa = 11.84 RR56 pKa = 11.84 RR57 pKa = 11.84 SRR59 pKa = 11.84 PASTTTRR66 pKa = 11.84 VMLVRR71 pKa = 11.84 TSRR74 pKa = 11.84 RR75 pKa = 11.84 RR76 pKa = 11.84 RR77 pKa = 11.84 RR78 pKa = 3.32
Molecular weight: 9.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.301
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.574
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.296
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.258
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
36
1
37
12846
52
1306
347.2
39.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.964 ± 0.349
1.861 ± 0.277
5.535 ± 0.254
6.274 ± 0.508
4.188 ± 0.249
6.189 ± 0.299
2.258 ± 0.238
4.056 ± 0.18
3.69 ± 0.322
8.765 ± 0.358
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.359 ± 0.162
4.227 ± 0.403
6.547 ± 0.262
3.596 ± 0.251
7.372 ± 0.519
7.216 ± 0.434
6.056 ± 0.398
6.485 ± 0.301
1.347 ± 0.114
4.017 ± 0.277
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here