Luteimicrobium subarcticum
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3150 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2M8W727|A0A2M8W727_9MICO Putative PIG3 family NAD(P)H quinone oxidoreductase OS=Luteimicrobium subarcticum OX=620910 GN=CLV34_2658 PE=4 SV=1
MM1 pKa = 7.12 LQADD5 pKa = 3.69 GVMQEE10 pKa = 3.93 RR11 pKa = 11.84 NAVIVRR17 pKa = 11.84 RR18 pKa = 11.84 ALGLVAVTALVSSALVGCSRR38 pKa = 11.84 GPDD41 pKa = 3.46 AKK43 pKa = 10.08 TLMHH47 pKa = 6.51 NCVAYY52 pKa = 10.08 HH53 pKa = 5.42 YY54 pKa = 9.68 DD55 pKa = 3.08 GWGAEE60 pKa = 4.22 AARR63 pKa = 11.84 TVCQVEE69 pKa = 4.86 FDD71 pKa = 4.14 DD72 pKa = 4.18 QPAYY76 pKa = 10.19 SACKK80 pKa = 9.56 YY81 pKa = 10.81 AEE83 pKa = 4.62 ADD85 pKa = 3.42 PAIVDD90 pKa = 4.2 RR91 pKa = 11.84 EE92 pKa = 4.07 AAANDD97 pKa = 4.42 CDD99 pKa = 4.92 AKK101 pKa = 10.74 WIDD104 pKa = 4.19 GGAATQGSAGGADD117 pKa = 3.66 GASDD121 pKa = 4.39 ADD123 pKa = 4.1 DD124 pKa = 4.6 DD125 pKa = 5.26 ADD127 pKa = 4.18 VAEE130 pKa = 5.19 EE131 pKa = 4.09 PQAPAPAPAASTDD144 pKa = 3.28 TSADD148 pKa = 3.26 AGDD151 pKa = 4.08 APEE154 pKa = 5.06 PPVADD159 pKa = 4.86 DD160 pKa = 4.63 EE161 pKa = 4.89 GDD163 pKa = 3.7 PMAVPAGGGVPTGVEE178 pKa = 4.14 GVVQDD183 pKa = 4.87 FLQATQGADD192 pKa = 2.82 VDD194 pKa = 4.38 LAIDD198 pKa = 3.53 VLGAPGAGLSDD209 pKa = 3.24 MFGTYY214 pKa = 9.91 GGRR217 pKa = 11.84 APYY220 pKa = 9.36 TKK222 pKa = 10.49 SLGCALGPDD231 pKa = 3.62 GRR233 pKa = 11.84 AYY235 pKa = 11.0 LCDD238 pKa = 3.6 VTNGTDD244 pKa = 3.47 PLEE247 pKa = 3.99 LVIASNADD255 pKa = 3.04 HH256 pKa = 7.05 AMWLLDD262 pKa = 3.56 SLYY265 pKa = 11.08 SADD268 pKa = 4.3 PEE270 pKa = 4.22 NN271 pKa = 3.64
Molecular weight: 27.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.695
IPC2_protein 3.656
IPC_protein 3.694
Toseland 3.452
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.363
Solomon 3.694
Lehninger 3.656
Nozaki 3.808
DTASelect 4.101
Thurlkill 3.516
EMBOSS 3.668
Sillero 3.821
Patrickios 0.896
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.746
Protein with the highest isoelectric point:
>tr|A0A2M8WVZ6|A0A2M8WVZ6_9MICO Ribonuclease VapC OS=Luteimicrobium subarcticum OX=620910 GN=vapC PE=3 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.42 KK7 pKa = 8.47 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.22 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.56 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3150
0
3150
1102968
30
3286
350.1
37.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.192 ± 0.054
0.559 ± 0.013
6.878 ± 0.043
4.751 ± 0.049
2.536 ± 0.028
9.479 ± 0.039
2.104 ± 0.021
2.685 ± 0.037
1.695 ± 0.032
9.892 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.421 ± 0.017
1.532 ± 0.023
5.901 ± 0.036
2.587 ± 0.02
7.616 ± 0.066
5.353 ± 0.041
6.889 ± 0.072
10.498 ± 0.054
1.549 ± 0.018
1.883 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here