Corallococcus coralloides (strain ATCC 25202 / DSM 2259 / NBRC 100086 / M2) (Myxococcus coralloides)
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8019 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H8N2B4|H8N2B4_CORCM Ribulose-phosphate 3-epimerase OS=Corallococcus coralloides (strain ATCC 25202 / DSM 2259 / NBRC 100086 / M2) OX=1144275 GN=rpe PE=3 SV=1
MM1 pKa = 7.33 QLDD4 pKa = 3.95 DD5 pKa = 3.86 QQTPNLTSTGVNGATGTNGAPGGPGGTGTQGPSGSDD41 pKa = 3.79 GSCGACNSQPGNGGTGGTGITGGDD65 pKa = 3.31 ASAGTTGLDD74 pKa = 3.33 APDD77 pKa = 4.52 VIFQVTNISGNFLVAVGGGDD97 pKa = 3.33 GGAGGTGGAGGQGGAGGPAGNTSNNCTQAANQGAGGKK134 pKa = 9.62 GGNGGKK140 pKa = 10.16 GGNGGNGGNGGTVFIYY156 pKa = 10.7 YY157 pKa = 7.82 QTLTPGSSTQLVRR170 pKa = 11.84 TVGQGGAAGQGGAAGSPGQGNGNSGSGSPGSNGAPGSNGTQGQVQFVQSSNTSLFII226 pKa = 5.31
Molecular weight: 20.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.884
IPC_protein 3.77
Toseland 3.554
ProMoST 4.024
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.872
Rodwell 3.617
Grimsley 3.478
Solomon 3.783
Lehninger 3.745
Nozaki 3.973
DTASelect 4.291
Thurlkill 3.668
EMBOSS 3.859
Sillero 3.91
Patrickios 0.604
IPC_peptide 3.77
IPC2_peptide 3.872
IPC2.peptide.svr19 3.812
Protein with the highest isoelectric point:
>tr|H8MMJ8|H8MMJ8_CORCM Succinyl-diaminopimelate desuccinylase OS=Corallococcus coralloides (strain ATCC 25202 / DSM 2259 / NBRC 100086 / M2) OX=1144275 GN=dapE2 PE=4 SV=1
MM1 pKa = 7.19 HH2 pKa = 7.68 AEE4 pKa = 4.91 SNMQDD9 pKa = 3.57 PNEE12 pKa = 4.35 TPSSNTMLPVGEE24 pKa = 4.27 ATPSRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 SSGARR36 pKa = 11.84 KK37 pKa = 8.99 GARR40 pKa = 11.84 KK41 pKa = 9.65 ASSRR45 pKa = 11.84 RR46 pKa = 11.84 TGAAKK51 pKa = 10.33 KK52 pKa = 8.82 ATSKK56 pKa = 9.95 RR57 pKa = 11.84 TTAKK61 pKa = 9.97 KK62 pKa = 10.19 AAGKK66 pKa = 8.51 RR67 pKa = 11.84 TAKK70 pKa = 10.13 KK71 pKa = 10.03 AAGKK75 pKa = 10.33 RR76 pKa = 11.84 GTAKK80 pKa = 10.42 RR81 pKa = 11.84 GAAKK85 pKa = 10.3 KK86 pKa = 9.06 ATAKK90 pKa = 10.31 RR91 pKa = 11.84 GARR94 pKa = 11.84 KK95 pKa = 9.77 APARR99 pKa = 11.84 RR100 pKa = 11.84 AAAGRR105 pKa = 11.84 TARR108 pKa = 11.84 KK109 pKa = 7.37 TSARR113 pKa = 11.84 KK114 pKa = 9.39 SPARR118 pKa = 11.84 KK119 pKa = 7.25 TSARR123 pKa = 11.84 KK124 pKa = 9.21 SSARR128 pKa = 11.84 RR129 pKa = 11.84 STRR132 pKa = 11.84 RR133 pKa = 3.13
Molecular weight: 14.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.397
IPC2_protein 10.862
IPC_protein 12.427
Toseland 12.588
ProMoST 13.086
Dawson 12.603
Bjellqvist 12.588
Wikipedia 13.071
Rodwell 12.398
Grimsley 12.632
Solomon 13.086
Lehninger 12.983
Nozaki 12.588
DTASelect 12.588
Thurlkill 12.588
EMBOSS 13.086
Sillero 12.588
Patrickios 12.106
IPC_peptide 13.086
IPC2_peptide 12.076
IPC2.peptide.svr19 9.056
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8019
0
8019
3071555
39
12261
383.0
41.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.146 ± 0.041
0.902 ± 0.014
5.275 ± 0.022
6.202 ± 0.032
3.361 ± 0.016
8.725 ± 0.031
2.101 ± 0.012
2.81 ± 0.02
3.063 ± 0.028
10.959 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.933 ± 0.011
2.167 ± 0.02
6.238 ± 0.028
3.509 ± 0.019
7.745 ± 0.03
5.559 ± 0.021
5.644 ± 0.024
8.201 ± 0.03
1.395 ± 0.011
2.064 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here