Cronobacter phage Dev-CD-23823
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K8IWK8|A0A0K8IWK8_9CAUD Uncharacterized protein OS=Cronobacter phage Dev-CD-23823 OX=1712539 GN=gp23 PE=4 SV=1
MM1 pKa = 7.96 DD2 pKa = 4.48 MKK4 pKa = 11.08 NVIDD8 pKa = 4.61 ILRR11 pKa = 11.84 EE12 pKa = 3.91 HH13 pKa = 6.41 NPEE16 pKa = 3.68 LKK18 pKa = 10.7 LRR20 pKa = 11.84 VGEE23 pKa = 3.99 LMQDD27 pKa = 3.24 IAEE30 pKa = 4.21 QMCGSVNCVGIHH42 pKa = 7.19 CDD44 pKa = 3.2 DD45 pKa = 5.53 CPFQRR50 pKa = 11.84 QNDD53 pKa = 3.87 PDD55 pKa = 3.98 DD56 pKa = 4.01 AVAVMGSMIEE66 pKa = 3.86 EE67 pKa = 4.57 MYY69 pKa = 11.19
Molecular weight: 7.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.111
IPC2_protein 4.202
IPC_protein 4.101
Toseland 3.91
ProMoST 4.253
Dawson 4.088
Bjellqvist 4.24
Wikipedia 4.012
Rodwell 3.935
Grimsley 3.821
Solomon 4.075
Lehninger 4.024
Nozaki 4.202
DTASelect 4.406
Thurlkill 3.961
EMBOSS 4.024
Sillero 4.228
Patrickios 3.058
IPC_peptide 4.075
IPC2_peptide 4.202
IPC2.peptide.svr19 4.126
Protein with the highest isoelectric point:
>tr|A0A0K8IXB5|A0A0K8IXB5_9CAUD DNA-directed RNA polymerase OS=Cronobacter phage Dev-CD-23823 OX=1712539 GN=gp29 PE=3 SV=1
MM1 pKa = 7.8 RR2 pKa = 11.84 KK3 pKa = 8.32 LTRR6 pKa = 11.84 SQAATLAAKK15 pKa = 8.66 MVRR18 pKa = 11.84 EE19 pKa = 4.13 STGCPLCQRR28 pKa = 11.84 TWAEE32 pKa = 4.02 VQKK35 pKa = 10.88 DD36 pKa = 4.05 AEE38 pKa = 4.57 DD39 pKa = 3.34 KK40 pKa = 11.08 AAAKK44 pKa = 10.02 GKK46 pKa = 10.06 KK47 pKa = 9.07 LRR49 pKa = 11.84 QAPYY53 pKa = 11.05 VLDD56 pKa = 4.21 HH57 pKa = 6.96 DD58 pKa = 5.12 HH59 pKa = 7.36 NSGRR63 pKa = 11.84 CRR65 pKa = 11.84 GVLCRR70 pKa = 11.84 GCNGAEE76 pKa = 3.73 GKK78 pKa = 9.63 VANAVSAWGKK88 pKa = 7.69 TGKK91 pKa = 10.14 EE92 pKa = 3.67 YY93 pKa = 10.96 AAILGWLQRR102 pKa = 11.84 MVAYY106 pKa = 9.67 LQQEE110 pKa = 4.25 PTEE113 pKa = 4.5 YY114 pKa = 9.87 IYY116 pKa = 8.46 PTHH119 pKa = 7.79 VMADD123 pKa = 3.74 EE124 pKa = 4.52 KK125 pKa = 11.02 KK126 pKa = 10.47 KK127 pKa = 10.93 SAGQLRR133 pKa = 11.84 RR134 pKa = 11.84 QAAQAKK140 pKa = 8.47 VRR142 pKa = 11.84 ARR144 pKa = 11.84 RR145 pKa = 11.84 KK146 pKa = 9.78 QIADD150 pKa = 3.3 KK151 pKa = 10.71 RR152 pKa = 11.84 GKK154 pKa = 9.94
Molecular weight: 17.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.212
IPC2_protein 9.414
IPC_protein 9.443
Toseland 10.35
ProMoST 9.94
Dawson 10.467
Bjellqvist 10.101
Wikipedia 10.584
Rodwell 10.994
Grimsley 10.496
Solomon 10.496
Lehninger 10.482
Nozaki 10.35
DTASelect 10.072
Thurlkill 10.35
EMBOSS 10.73
Sillero 10.379
Patrickios 10.701
IPC_peptide 10.496
IPC2_peptide 8.887
IPC2.peptide.svr19 8.367
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
48
0
48
12996
38
1260
270.8
30.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.303 ± 0.493
0.993 ± 0.148
6.24 ± 0.202
6.448 ± 0.339
3.447 ± 0.159
7.579 ± 0.306
1.862 ± 0.159
4.825 ± 0.274
6.179 ± 0.336
8.133 ± 0.359
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.032 ± 0.203
4.209 ± 0.263
3.932 ± 0.198
4.748 ± 0.437
5.586 ± 0.179
5.155 ± 0.219
5.54 ± 0.296
6.679 ± 0.322
1.485 ± 0.103
3.624 ± 0.218
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here