Novosphingobium kunmingense

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3342 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N0H306|A0A2N0H306_9SPHN NADPH:quinone reductase-like Zn-dependent oxidoreductase OS=Novosphingobium kunmingense OX=1211806 GN=B0I00_3346 PE=4 SV=1
MM1 pKa = 7.16AQFWHH6 pKa = 6.34NFAWWYY12 pKa = 9.77SLPITITDD20 pKa = 3.42TSGNDD25 pKa = 3.76AISGTDD31 pKa = 3.53GADD34 pKa = 3.47HH35 pKa = 7.14ISGNSGDD42 pKa = 4.99DD43 pKa = 3.75EE44 pKa = 4.36IAGLGGDD51 pKa = 4.82DD52 pKa = 3.71YY53 pKa = 11.97LEE55 pKa = 4.7GGNEE59 pKa = 3.6IDD61 pKa = 3.64TLYY64 pKa = 11.27GGAGNDD70 pKa = 3.4MLDD73 pKa = 3.63PGRR76 pKa = 11.84DD77 pKa = 3.0QDD79 pKa = 4.02TVYY82 pKa = 10.96GGPDD86 pKa = 3.24DD87 pKa = 5.7DD88 pKa = 5.18YY89 pKa = 11.31YY90 pKa = 11.48LIYY93 pKa = 10.3GAEE96 pKa = 4.08AAAQDD101 pKa = 4.17TLIEE105 pKa = 4.2AANEE109 pKa = 3.82GFDD112 pKa = 4.37WIRR115 pKa = 11.84SSQDD119 pKa = 2.91GYY121 pKa = 11.48GLPFNFEE128 pKa = 4.12GLFLHH133 pKa = 7.1IDD135 pKa = 3.59GGGGLAYY142 pKa = 10.36DD143 pKa = 4.21GSGNEE148 pKa = 4.1LDD150 pKa = 3.96NVIVGQGLANHH161 pKa = 6.8IFGLGGNDD169 pKa = 3.51LLLGEE174 pKa = 5.4GGNDD178 pKa = 3.2WLDD181 pKa = 3.76GGSGSDD187 pKa = 3.12WLEE190 pKa = 4.07GGLGSDD196 pKa = 3.49WFVGSTGNDD205 pKa = 2.83VMLGDD210 pKa = 4.64GGSDD214 pKa = 3.28TFDD217 pKa = 3.13LRR219 pKa = 11.84AVTFVAGQSVLIDD232 pKa = 3.78GAGSTGDD239 pKa = 3.74PASPDD244 pKa = 2.97VDD246 pKa = 3.64TLVLTGRR253 pKa = 11.84ATDD256 pKa = 3.36WTFTTTFEE264 pKa = 5.92DD265 pKa = 4.8GVQPTRR271 pKa = 11.84TVLTNNLTGTLLYY284 pKa = 9.01TQHH287 pKa = 7.64LEE289 pKa = 4.13ALSFTAPVNNLVNLYY304 pKa = 9.9QGNLVLEE311 pKa = 5.22LIRR314 pKa = 11.84LAGEE318 pKa = 4.13SYY320 pKa = 11.01GALEE324 pKa = 4.36TLDD327 pKa = 4.72HH328 pKa = 6.43LAEE331 pKa = 4.71PLASTGTFAAWAAAGVAAEE350 pKa = 4.23ATQLRR355 pKa = 11.84HH356 pKa = 4.12WHH358 pKa = 6.31ALSALEE364 pKa = 4.3LGIPAAYY371 pKa = 7.7THH373 pKa = 6.81PQTGLQYY380 pKa = 11.25SLTNGHH386 pKa = 5.76YY387 pKa = 9.32QAIYY391 pKa = 8.31PSPVGIVDD399 pKa = 3.57TSEE402 pKa = 4.18ANALVLIGVVNGVTTLALAFRR423 pKa = 11.84GTDD426 pKa = 3.25QFADD430 pKa = 3.78FADD433 pKa = 4.25YY434 pKa = 11.03PDD436 pKa = 3.82FTLDD440 pKa = 3.4HH441 pKa = 5.89YY442 pKa = 11.97AKK444 pKa = 10.46FKK446 pKa = 10.61PLIDD450 pKa = 5.58AIDD453 pKa = 4.37AYY455 pKa = 11.32VADD458 pKa = 4.71PANGIQQVLVSGHH471 pKa = 5.48SLGAGMVQEE480 pKa = 4.88FMSDD484 pKa = 3.07HH485 pKa = 6.67AGSLYY490 pKa = 10.14QAVTIGSPGSDD501 pKa = 3.39NNDD504 pKa = 3.81GIEE507 pKa = 4.33DD508 pKa = 3.84DD509 pKa = 3.76PRR511 pKa = 11.84IVNFAHH517 pKa = 6.43TSDD520 pKa = 4.1AVAMIAPALSQAAPILSAWLLLQGQVTLAAIIGKK554 pKa = 8.46IQPKK558 pKa = 10.34DD559 pKa = 3.47RR560 pKa = 11.84DD561 pKa = 3.63GSDD564 pKa = 3.32VYY566 pKa = 11.54VNSDD570 pKa = 2.55IATFPGITEE579 pKa = 4.33HH580 pKa = 7.2AWTNYY585 pKa = 8.61LASVTTILEE594 pKa = 4.19RR595 pKa = 11.84AVTGPFSLHH604 pKa = 6.3PFAAALRR611 pKa = 11.84AEE613 pKa = 4.48LVYY616 pKa = 11.12SGDD619 pKa = 3.9DD620 pKa = 3.36VQIAIGQPGSSKK632 pKa = 8.56ITSWTGDD639 pKa = 3.3NFVLGSSAADD649 pKa = 3.51SIEE652 pKa = 3.96WSGVTPLGEE661 pKa = 4.07YY662 pKa = 10.06RR663 pKa = 11.84VIDD666 pKa = 3.9AGLGSDD672 pKa = 3.96EE673 pKa = 5.87LILPGLKK680 pKa = 10.43ASWGWDD686 pKa = 3.21NLGGHH691 pKa = 6.11YY692 pKa = 10.65ALSFLGLDD700 pKa = 3.93VGEE703 pKa = 4.28LWNVEE708 pKa = 3.93KK709 pKa = 10.95LVFTFFSDD717 pKa = 4.94EE718 pKa = 4.05EE719 pKa = 4.13LQQAKK724 pKa = 8.18TAFAAAEE731 pKa = 4.15PAPVIPVVYY740 pKa = 10.48LNGQAASVQQAVPGASVLNVDD761 pKa = 3.81PDD763 pKa = 3.27VDD765 pKa = 4.03YY766 pKa = 11.94VEE768 pKa = 5.02TGNSTLMVNGSADD781 pKa = 3.26NDD783 pKa = 3.8IIMLGGGNQTIDD795 pKa = 3.12GGAGNDD801 pKa = 3.29VVNAVNAASGGVFAVTGGGGDD822 pKa = 3.88DD823 pKa = 4.05LVIGSGGARR832 pKa = 11.84MKK834 pKa = 10.84AVFSGNAADD843 pKa = 3.93YY844 pKa = 10.72GADD847 pKa = 3.37EE848 pKa = 5.24RR849 pKa = 11.84GDD851 pKa = 3.5GGIILTDD858 pKa = 4.24LRR860 pKa = 11.84PGSPDD865 pKa = 3.6GEE867 pKa = 4.24DD868 pKa = 2.97TLLQVVTLRR877 pKa = 11.84FADD880 pKa = 3.75GDD882 pKa = 3.47ITAAQFLAEE891 pKa = 4.39RR892 pKa = 11.84VPVLLQGTDD901 pKa = 3.9DD902 pKa = 5.18DD903 pKa = 5.1DD904 pKa = 5.83ALIPARR910 pKa = 11.84DD911 pKa = 3.73GTVIAIGLAGDD922 pKa = 4.27DD923 pKa = 4.06SLEE926 pKa = 4.28GGARR930 pKa = 11.84ADD932 pKa = 4.21LLDD935 pKa = 4.33GGTGADD941 pKa = 3.62HH942 pKa = 7.43LYY944 pKa = 10.92GHH946 pKa = 7.38AGDD949 pKa = 5.92DD950 pKa = 3.71MYY952 pKa = 11.62LVNQLADD959 pKa = 3.56TVFEE963 pKa = 4.58GDD965 pKa = 3.77GEE967 pKa = 4.55GTDD970 pKa = 3.47TVTASGNFYY979 pKa = 10.77LYY981 pKa = 11.23ANIEE985 pKa = 4.22NLVQAAGAA993 pKa = 3.7

Molecular weight: 103.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N0H6X2|A0A2N0H6X2_9SPHN Na+/H+-dicarboxylate symporter OS=Novosphingobium kunmingense OX=1211806 GN=B0I00_2286 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.54GFRR19 pKa = 11.84ARR21 pKa = 11.84TATVGGRR28 pKa = 11.84KK29 pKa = 8.17VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3342

0

3342

1082236

29

3423

323.8

34.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.603 ± 0.07

0.844 ± 0.013

6.071 ± 0.042

5.337 ± 0.039

3.543 ± 0.025

9.034 ± 0.04

1.945 ± 0.021

4.724 ± 0.027

2.969 ± 0.03

10.02 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.366 ± 0.022

2.53 ± 0.038

5.316 ± 0.03

3.114 ± 0.021

7.237 ± 0.051

5.181 ± 0.03

5.245 ± 0.048

7.266 ± 0.033

1.482 ± 0.018

2.174 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski